This is a Validated Antibody Database (VAD) review about dog PRKCA, based on 28 published articles (read how Labome selects the articles), using PRKCA antibody in all methods. It is aimed to help Labome visitors find the most suited PRKCA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PRKCA synonym: protein kinase C alpha type

Knockout validation
Cell Signaling Technology
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 6a
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot knockout validation on mouse samples (fig 6a) and in western blot on human samples (fig 2a). PLoS Pathog (2017) ncbi
Novus Biologicals
mouse monoclonal (MC5)
  • immunohistochemistry - frozen section; mouse; 1:1000
Novus Biologicals PRKCA antibody (Novus, NB600-201) was used in immunohistochemistry - frozen section on mouse samples at 1:1000. J Comp Neurol (2007) ncbi
Invitrogen
mouse monoclonal (MC5)
  • immunohistochemistry; rabbit; 1:100; fig 2
  • immunohistochemistry; mouse; 1:100; fig 2
  • immunohistochemistry; dog; 1:100; fig 2
  • immunohistochemistry; hamsters; 1:100; fig 2
In order to test if the alpha1c subunit of L-type voltage-gated calcium channel colocalizes with protein kinase C alpha in the rod bipolar cells in mouse, hamster, rabbit, and dog retinas, Invitrogen PRKCA antibody (Thermo Fisher, MA1-157) was used in immunohistochemistry on rabbit samples at 1:100 (fig 2), in immunohistochemistry on mouse samples at 1:100 (fig 2), in immunohistochemistry on dog samples at 1:100 (fig 2) and in immunohistochemistry on hamsters samples at 1:100 (fig 2). Acta Histochem Cytochem (2015) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; loading ...; fig 3e
Cell Signaling Technology PRKCA antibody (Cell Signaling Technology, 2056) was used in western blot on human samples (fig 3e). Cell Metab (2019) ncbi
rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 3b
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples at 1:1000 (fig 3b). Cancer Res (2018) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 3
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on mouse samples at 1:1000 (fig 3). Exp Neurol (2018) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 1b
In order to study the effect of GNAQ mutations and GNA11/MAPK pathway activation in uveal melanoma, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples (fig 1b). Cancer Cell (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...
Cell Signaling Technology PRKCA antibody (Cell Signaling Technology, 2056) was used in western blot on human samples . Neoplasia (2017) ncbi
rabbit polyclonal
  • western blot knockout validation; mouse; loading ...; fig 6a
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot knockout validation on mouse samples (fig 6a) and in western blot on human samples (fig 2a). PLoS Pathog (2017) ncbi
rabbit polyclonal
  • western blot; human; loading ...; fig 2b
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples (fig 2b). Oncotarget (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:500; fig s3
In order to study the contribution of TRPM2 in hyperglycemia-induced oxidative stress, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056 S) was used in western blot on human samples at 1:500 (fig s3). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 3
In order to investigate the contribution of DNA methyltransferase 1 to the epithelial-mesenchymal transition and cancer stem cells, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples at 1:1000 (fig 3). Neoplasia (2016) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 7a
In order to demonstrate that Siglec-E is required for Escherichia coli-induced endocytosis of TLR4, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on mouse samples (fig 7a). J Immunol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5e
In order to ascertain the mechanism of action of the Rb9 peptide, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on mouse samples (fig 5e). Peptides (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig st2
Cell Signaling Technology PRKCA antibody (Cell signaling, 2056) was used in western blot on mouse samples at 1:1000 (fig st2). PLoS ONE (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5
Cell Signaling Technology PRKCA antibody (Cell Signaling Technology, 2056) was used in western blot on mouse samples at 1:1000 (fig 5). Cell Rep (2016) ncbi
rabbit polyclonal
  • western blot; human; fig s15
Cell Signaling Technology PRKCA antibody (Cell Signaling Tech, 2056P) was used in western blot on human samples (fig s15). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; 1:50; fig 6
In order to study syndecan-4-mediated podocyte cell-matrix interactions that require N-sulfation of heparan sulfate, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in immunocytochemistry on mouse samples at 1:50 (fig 6). Am J Physiol Renal Physiol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 6e
Cell Signaling Technology PRKCA antibody (Cell Signaling, cs-2056) was used in western blot on mouse samples at 1:1000 (fig 6e). EMBO Mol Med (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples (fig 5). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 6c
Cell Signaling Technology PRKCA antibody (Cell Signaling Technology, 2056) was used in western blot on mouse samples at 1:1000 (fig 6c). Endocrinology (2015) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse
In order to study NGF in E-Reeler retinogenesis, Cell Signaling Technology PRKCA antibody (Cell Signalling Technology, 2056S) was used in immunocytochemistry on mouse samples . Neuromolecular Med (2015) ncbi
rabbit polyclonal
  • western blot; mouse; fig 4
Cell Signaling Technology PRKCA antibody (Cell Signaling Technology, 2056) was used in western blot on mouse samples (fig 4). Kidney Int (2015) ncbi
rabbit polyclonal
  • western blot; human
In order to identify small-molecules that inhibit the interaction between CDK5 and p25, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples . Chem Biol (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig s1e
In order to elucidate the effect of RAB39B on synaptic activity, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on mouse samples at 1:1000 (fig s1e). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 9
  • western blot; mouse; fig 2
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples (fig 9) and in western blot on mouse samples (fig 2). Mol Cell Biol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200; loading ...; fig 3l
Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056S) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig 3l). Dev Dyn (2015) ncbi
rabbit polyclonal
  • western blot; human
In order to study how phosphorylation alters COP1-binding sites of oncoproteins, Cell Signaling Technology PRKCA antibody (Cell Signaling, 2056) was used in western blot on human samples . Cancer Cell (2014) ncbi
Articles Reviewed
  1. Son S, Park S, Lee H, Siddiqi F, Lee J, Menzies F, et al. Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A. Cell Metab. 2019;29:192-201.e7 pubmed publisher
  2. Ng P, Li J, Jeong K, Shao S, Chen H, Tsang Y, et al. Systematic Functional Annotation of Somatic Mutations in Cancer. Cancer Cell. 2018;33:450-462.e10 pubmed publisher
  3. Jung Y, Cackowski F, Yumoto K, Decker A, Wang J, Kim J, et al. CXCL12γ Promotes Metastatic Castration-Resistant Prostate Cancer by Inducing Cancer Stem Cell and Neuroendocrine Phenotypes. Cancer Res. 2018;78:2026-2039 pubmed publisher
  4. Lu F, Shao G, Wang Y, Guan S, Burlingame A, Liu X, et al. Hypoxia-ischemia modifies postsynaptic GluN2B-containing NMDA receptor complexes in the neonatal mouse brain. Exp Neurol. 2018;299:65-74 pubmed publisher
  5. Chen X, Wu Q, Depeille P, Chen P, Thornton S, Kalirai H, et al. RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma. Cancer Cell. 2017;31:685-696.e6 pubmed publisher
  6. Zhai S, Liu C, Zhang L, Zhu J, Guo J, Zhang J, et al. PLCE1 Promotes Esophageal Cancer Cell Progression by Maintaining the Transcriptional Activity of Snail. Neoplasia. 2017;19:154-164 pubmed publisher
  7. Koh H, Kim Y, Kim J, Yun J, Jang K, Yang C. Toxoplasma gondii GRA7-Targeted ASC and PLD1 Promote Antibacterial Host Defense via PKCα. PLoS Pathog. 2017;13:e1006126 pubmed publisher
  8. Sakre N, Wildey G, Behtaj M, Kresak A, Yang M, Fu P, et al. RICTOR amplification identifies a subgroup in small cell lung cancer and predicts response to drugs targeting mTOR. Oncotarget. 2017;8:5992-6002 pubmed publisher
  9. Tseng H, Vong C, Kwan Y, Lee S, Hoi M. TRPM2 regulates TXNIP-mediated NLRP3 inflammasome activation via interaction with p47 phox under high glucose in human monocytic cells. Sci Rep. 2016;6:35016 pubmed publisher
  10. Lee E, Wang J, Yumoto K, Jung Y, Cackowski F, Decker A, et al. DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis. Neoplasia. 2016;18:553-66 pubmed publisher
  11. Wu Y, Ren D, Chen G. Siglec-E Negatively Regulates the Activation of TLR4 by Controlling Its Endocytosis. J Immunol. 2016;197:3336-3347 pubmed
  12. Girola N, Matsuo A, Figueiredo C, Massaoka M, Farias C, Arruda D, et al. The Ig VH complementarity-determining region 3-containing Rb9 peptide, inhibits melanoma cells migration and invasion by interactions with Hsp90 and an adhesion G-protein coupled receptor. Peptides. 2016;85:1-15 pubmed publisher
  13. Gharib M, Tao H, Fungwe T, Hajri T. Cluster Differentiating 36 (CD36) Deficiency Attenuates Obesity-Associated Oxidative Stress in the Heart. PLoS ONE. 2016;11:e0155611 pubmed publisher
  14. Najibi M, Labed S, Visvikis O, IRAZOQUI J. An Evolutionarily Conserved PLC-PKD-TFEB Pathway for Host Defense. Cell Rep. 2016;15:1728-42 pubmed publisher
  15. Weigel C, Veldwijk M, Oakes C, Seibold P, Slynko A, Liesenfeld D, et al. Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis. Nat Commun. 2016;7:10893 pubmed publisher
  16. Sugar T, Wassenhove McCarthy D, Orr A, Green J, van Kuppevelt T, McCarthy K. N-sulfation of heparan sulfate is critical for syndecan-4-mediated podocyte cell-matrix interactions. Am J Physiol Renal Physiol. 2016;310:F1123-35 pubmed publisher
  17. Albert V, Svensson K, Shimobayashi M, Colombi M, Munoz S, Jimenez V, et al. mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue. EMBO Mol Med. 2016;8:232-46 pubmed publisher
  18. Yasuda K, Takahashi M, Mori N. Mdm20 Modulates Actin Remodeling through the mTORC2 Pathway via Its Effect on Rictor Expression. PLoS ONE. 2015;10:e0142943 pubmed publisher
  19. Dong H, Chen Z, Wang C, Xiong Z, Zhao W, Jia C, et al. Rictor Regulates Spermatogenesis by Controlling Sertoli Cell Cytoskeletal Organization and Cell Polarity in the Mouse Testis. Endocrinology. 2015;156:4244-56 pubmed publisher
  20. Balzamino B, Esposito G, Marino R, Keller F, Micera A. NGF Expression in Reelin-Deprived Retinal Cells: A Potential Neuroprotective Effect. Neuromolecular Med. 2015;17:314-25 pubmed publisher
  21. Huh Y, Choi J, Jeon C. Localization of Rod Bipolar Cells in the Mammalian Retina Using an Antibody Against the α1c L-type Ca(2+) Channel. Acta Histochem Cytochem. 2015;48:47-52 pubmed publisher
  22. Li J, Ren J, Liu X, Jiang L, He W, Yuan W, et al. Rictor/mTORC2 signaling mediates TGFβ1-induced fibroblast activation and kidney fibrosis. Kidney Int. 2015;88:515-27 pubmed publisher
  23. Corbel C, Zhang B, Le Parc A, Baratte B, Colas P, Couturier C, et al. Tamoxifen inhibits CDK5 kinase activity by interacting with p35/p25 and modulates the pattern of tau phosphorylation. Chem Biol. 2015;22:472-482 pubmed publisher
  24. Mignogna M, Giannandrea M, Gurgone A, Fanelli F, Raimondi F, Mapelli L, et al. The intellectual disability protein RAB39B selectively regulates GluA2 trafficking to determine synaptic AMPAR composition. Nat Commun. 2015;6:6504 pubmed publisher
  25. Wang S, Amato K, Song W, Youngblood V, Lee K, Boothby M, et al. Regulation of endothelial cell proliferation and vascular assembly through distinct mTORC2 signaling pathways. Mol Cell Biol. 2015;35:1299-313 pubmed publisher
  26. Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher
  27. Lu G, Zhang Q, Huang Y, Song J, Tomaino R, Ehrenberger T, et al. Phosphorylation of ETS1 by Src family kinases prevents its recognition by the COP1 tumor suppressor. Cancer Cell. 2014;26:222-34 pubmed publisher
  28. Bayley P, Morgans C. Rod bipolar cells and horizontal cells form displaced synaptic contacts with rods in the outer nuclear layer of the nob2 retina. J Comp Neurol. 2007;500:286-98 pubmed