This is a Validated Antibody Database (VAD) review about dog PRKACA, based on 37 published articles (read how Labome selects the articles), using PRKACA antibody in all methods. It is aimed to help Labome visitors find the most suited PRKACA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PRKACA synonym: cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; protein kinase A alpha; protein kinase, cAMP-dependent, catalytic, alpha

Knockout validation
BD Biosciences
mouse monoclonal (21/PKC)
  • western blot knockout validation; mouse; loading ...; fig s1c
BD Biosciences PRKACA antibody (BD Biosciences, 610085) was used in western blot knockout validation on mouse samples (fig s1c). Cell Rep (2018) ncbi
BD Biosciences
mouse monoclonal (3/PKC)
  • western blot knockout validation; mouse; loading ...; fig s1a
BD Biosciences PRKACA antibody (BD Biosciences, 610107) was used in western blot knockout validation on mouse samples (fig s1a). Cell Rep (2018) ncbi
BD Biosciences
mouse monoclonal (5B)
  • western blot knockout validation; human; 1:1000; loading ...; fig 3b
BD Biosciences PRKACA antibody (BD Biosciences, 5B) was used in western blot knockout validation on human samples at 1:1000 (fig 3b). Science (2017) ncbi
BD Biosciences
mouse monoclonal (23/PKC)
  • immunohistochemistry knockout validation; mouse; fig 2
In order to report a role for aPKClambda in the development of the lens fulcrum, BD Biosciences PRKACA antibody (TDL, 610175) was used in immunohistochemistry knockout validation on mouse samples (fig 2). Dev Biol (2009) ncbi
BD Biosciences
mouse monoclonal (23/PKC)
  • western blot knockout validation; mouse; 1:1000
In order to examine the role of atypical protein kinase C lambda in mouse neocortical neurogenesis, BD Biosciences PRKACA antibody (BD, 23) was used in western blot knockout validation on mouse samples at 1:1000. Development (2006) ncbi
Abcam
rabbit polyclonal
  • immunohistochemistry - frozen section; rat; 1:50; fig 5f
  • western blot; rat; 1:1000; fig 5a
In order to determine the role of spinal GABABRs in cancer-induced bone pain, Abcam PRKACA antibody (Abcam, ab26322) was used in immunohistochemistry - frozen section on rat samples at 1:50 (fig 5f) and in western blot on rat samples at 1:1000 (fig 5a). J Pain (2017) ncbi
rabbit polyclonal
  • western blot; mouse; 1:10,000; fig 1
In order to elucidate regulation of brown and beige adipocyte function via ASK1 signaling, Abcam PRKACA antibody (Abcam, ab26322) was used in western blot on mouse samples at 1:10,000 (fig 1). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; 1:1000; fig 2b
Abcam PRKACA antibody (Abcam, ab26322) was used in immunocytochemistry on human samples at 1:1000 (fig 2b). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 7
Abcam PRKACA antibody (abcam, ab26322) was used in immunocytochemistry on human samples (fig 7). Sci Rep (2015) ncbi
rabbit polyclonal
  • western blot; pig
Abcam PRKACA antibody (Abcam, ab26322) was used in western blot on pig samples . PLoS ONE (2015) ncbi
BD Biosciences
mouse monoclonal (3/PKC)
  • western blot knockout validation; mouse; loading ...; fig s1a
BD Biosciences PRKACA antibody (BD Biosciences, 610107) was used in western blot knockout validation on mouse samples (fig s1a). Cell Rep (2018) ncbi
mouse monoclonal (21/PKC)
  • western blot knockout validation; mouse; loading ...; fig s1c
BD Biosciences PRKACA antibody (BD Biosciences, 610085) was used in western blot knockout validation on mouse samples (fig s1c). Cell Rep (2018) ncbi
mouse monoclonal (5B)
  • western blot; human; 0.5 ug/ml; loading ...; fig 1d
BD Biosciences PRKACA antibody (BD Biosciences, 610981) was used in western blot on human samples at 0.5 ug/ml (fig 1d). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (5B)
  • western blot knockout validation; human; 1:1000; loading ...; fig 3b
BD Biosciences PRKACA antibody (BD Biosciences, 5B) was used in western blot knockout validation on human samples at 1:1000 (fig 3b). Science (2017) ncbi
mouse monoclonal (21/PKC)
  • western blot; human; loading ...; fig 1b
In order to study the effect of GNAQ mutations and GNA11/MAPK pathway activation in uveal melanoma, BD Biosciences PRKACA antibody (BD Bioscience, 610085) was used in western blot on human samples (fig 1b). Cancer Cell (2017) ncbi
mouse monoclonal (5B)
  • immunohistochemistry - paraffin section; human; 1:1000; fig 1a
  • western blot; human; 1:1000; fig 2a
BD Biosciences PRKACA antibody (BD, 610980) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 1a) and in western blot on human samples at 1:1000 (fig 2a). Sci Rep (2017) ncbi
mouse monoclonal (41/PKC)
  • immunocytochemistry; human; 1:100; loading ...; fig 3b
In order to show that the absence of EpCAM in enterocytes results in an aberrant apical domain, BD Biosciences PRKACA antibody (BD Biosciences, 610207) was used in immunocytochemistry on human samples at 1:100 (fig 3b). Nat Commun (2017) ncbi
mouse monoclonal (5B)
  • immunocytochemistry; human; 1:200; loading ...; fig 8g
  • western blot; human; 1:1000; loading ...; fig 4c
  • immunocytochemistry; dog; 1:200; loading ...; fig 4a
In order to elucidate the mechanisms by which ARHGAP36 blocks protein kinase A signaling, BD Biosciences PRKACA antibody (BD, 610981) was used in immunocytochemistry on human samples at 1:200 (fig 8g), in western blot on human samples at 1:1000 (fig 4c) and in immunocytochemistry on dog samples at 1:200 (fig 4a). Nat Commun (2016) ncbi
mouse monoclonal (5B)
  • western blot; mouse; 1:5000; loading ...; fig 1j
In order to examine the role of AKAP220 in actin dynamics and AQP2 trafficking, BD Biosciences PRKACA antibody (BD Biosciences, 610981) was used in western blot on mouse samples at 1:5000 (fig 1j). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (5B)
  • western blot; human; fig 1
BD Biosciences PRKACA antibody (BD Bioscience, 610981) was used in western blot on human samples (fig 1). Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (23/PKC)
  • immunohistochemistry - paraffin section; human; loading ...; fig 6c
  • western blot; human; 1:1000; fig 2a
BD Biosciences PRKACA antibody (BD Transduction Lab, 60175) was used in immunohistochemistry - paraffin section on human samples (fig 6c) and in western blot on human samples at 1:1000 (fig 2a). Int J Cancer (2016) ncbi
mouse monoclonal (5B)
  • immunohistochemistry; human; fig 6
BD Biosciences PRKACA antibody (BD Biosciences, 610981) was used in immunohistochemistry on human samples (fig 6). Pediatr Res (2016) ncbi
mouse monoclonal (3/PKC)
  • western blot; mouse; 1:1000; fig 7
  • western blot; human; 1:1000; fig 8
In order to analyze mediation of delayed myogenesis in Duchenne muscular dystrophy fetal muscle by inositol 1,4,5-triphosphate (IP3)-dependent Ca2+ signaling, BD Biosciences PRKACA antibody (BD Biosciences, 610107) was used in western blot on mouse samples at 1:1000 (fig 7) and in western blot on human samples at 1:1000 (fig 8). Development (2016) ncbi
mouse monoclonal (5B)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences PRKACA antibody (BD, 5B) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (21/PKC)
  • other; human; loading ...; fig st1
  • western blot; human; loading ...; fig st4
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences PRKACA antibody (BD, 21) was used in other on human samples (fig st1) and in western blot on human samples (fig st4). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (23/PKC)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, BD Biosciences PRKACA antibody (BD, 23) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (5B)
  • western blot; human; fig 3
BD Biosciences PRKACA antibody (BD Biosciences, 610980) was used in western blot on human samples (fig 3). Front Pharmacol (2015) ncbi
mouse monoclonal (5B)
  • western blot; mouse; 1:1000; fig 4e
BD Biosciences PRKACA antibody (BD Transduction Laboratories, 610980) was used in western blot on mouse samples at 1:1000 (fig 4e). Mol Metab (2015) ncbi
mouse monoclonal (41/PKC)
  • immunohistochemistry; mouse; 1:200; fig 4
In order to determine the precise role of actin in the neural epithelium, BD Biosciences PRKACA antibody (BD Biosciences, 610208) was used in immunohistochemistry on mouse samples at 1:200 (fig 4). Development (2015) ncbi
mouse monoclonal (5B)
  • western blot; human
In order to study PDE3 in human cardiac myocytes, BD Biosciences PRKACA antibody (BD Biosciences, 610981) was used in western blot on human samples . J Biol Chem (2015) ncbi
mouse monoclonal (5B)
  • western blot; human; 1:4000
In order to study two mutants of protein kinase A with enhanced catalytic activity, BD Biosciences PRKACA antibody (BD Transduction Laboratories, 610980) was used in western blot on human samples at 1:4000. Nat Commun (2014) ncbi
mouse monoclonal (3/PKC)
  • immunohistochemistry - free floating section; mouse; 1:250
BD Biosciences PRKACA antibody (BD Transduction Lab, 610107) was used in immunohistochemistry - free floating section on mouse samples at 1:250. Vision Res (2014) ncbi
mouse monoclonal (23/PKC)
  • immunohistochemistry - paraffin section; human; 1:100
BD Biosciences PRKACA antibody (BD Biosciences, 610176) was used in immunohistochemistry - paraffin section on human samples at 1:100. Anticancer Res (2014) ncbi
mouse monoclonal (3/PKC)
  • immunohistochemistry - free floating section; mouse; 1:250
BD Biosciences PRKACA antibody (BD Transduction Lab, 610107) was used in immunohistochemistry - free floating section on mouse samples at 1:250. Vision Res (2014) ncbi
mouse monoclonal (5B)
  • immunohistochemistry - paraffin section; human; 1:1000; fig 3
  • western blot; human; fig 2
BD Biosciences PRKACA antibody (BD Transduction Lab, 610980) was used in immunohistochemistry - paraffin section on human samples at 1:1000 (fig 3) and in western blot on human samples (fig 2). Oncogene (2015) ncbi
mouse monoclonal (23/PKC)
  • immunohistochemistry; human; 1:100
BD Biosciences PRKACA antibody (BD Biosciences, 610176) was used in immunohistochemistry on human samples at 1:100. Anticancer Res (2014) ncbi
mouse monoclonal (23/PKC)
  • western blot; mouse; fig 2
BD Biosciences PRKACA antibody (BD Transduction Laboratories, 610175) was used in western blot on mouse samples (fig 2). PLoS ONE (2013) ncbi
mouse monoclonal (41/PKC)
  • western blot; human
In order to determine the contribution of protein kinase C iota to normal-polarized mammalian morphogenesis, BD Biosciences PRKACA antibody (BD Biosciences, 610208) was used in western blot on human samples . Carcinogenesis (2014) ncbi
mouse monoclonal (5B)
  • immunohistochemistry - paraffin section; mouse
  • western blot; mouse; fig 1
BD Biosciences PRKACA antibody (BD Biosciences, 610980) was used in immunohistochemistry - paraffin section on mouse samples and in western blot on mouse samples (fig 1). PLoS ONE (2012) ncbi
mouse monoclonal (3/PKC)
  • immunohistochemistry - frozen section; mouse; 1:1000
BD Biosciences PRKACA antibody (Transduction Laboratories, 610107) was used in immunohistochemistry - frozen section on mouse samples at 1:1000. J Comp Neurol (2012) ncbi
mouse monoclonal (3/PKC)
  • immunohistochemistry - frozen section; mouse
BD Biosciences PRKACA antibody (BD Transduction Laboratories, 610108) was used in immunohistochemistry - frozen section on mouse samples . J Comp Neurol (2010) ncbi
mouse monoclonal (3/PKC)
  • immunohistochemistry; rat; 1:100
BD Biosciences PRKACA antibody (BD Transduction Laboratories, 610107) was used in immunohistochemistry on rat samples at 1:100. J Comp Neurol (2010) ncbi
mouse monoclonal (23/PKC)
  • immunohistochemistry knockout validation; mouse; fig 2
In order to report a role for aPKClambda in the development of the lens fulcrum, BD Biosciences PRKACA antibody (TDL, 610175) was used in immunohistochemistry knockout validation on mouse samples (fig 2). Dev Biol (2009) ncbi
mouse monoclonal (23/PKC)
  • western blot; mouse; fig 1
In order to investigate the role of aPKC kinase activity on actomyosin contraction, BD Biosciences PRKACA antibody (Becton Dickinson, 610176) was used in western blot on mouse samples (fig 1). J Cell Sci (2008) ncbi
mouse monoclonal (23/PKC)
  • western blot knockout validation; mouse; 1:1000
In order to examine the role of atypical protein kinase C lambda in mouse neocortical neurogenesis, BD Biosciences PRKACA antibody (BD, 23) was used in western blot knockout validation on mouse samples at 1:1000. Development (2006) ncbi
Articles Reviewed
  1. Schaffer T, Smith J, Cook E, Phan T, Margolis S. PKCε Inhibits Neuronal Dendritic Spine Development through Dual Phosphorylation of Ephexin5. Cell Rep. 2018;25:2470-2483.e8 pubmed publisher
  2. Walker Gray R, Stengel F, Gold M. Mechanisms for restraining cAMP-dependent protein kinase revealed by subunit quantitation and cross-linking approaches. Proc Natl Acad Sci U S A. 2017;114:10414-10419 pubmed publisher
  3. Smith F, Esseltine J, Nygren P, Veesler D, Byrne D, Vonderach M, et al. Local protein kinase A action proceeds through intact holoenzymes. Science. 2017;356:1288-1293 pubmed publisher
  4. Chen X, Wu Q, Depeille P, Chen P, Thornton S, Kalirai H, et al. RasGRP3 Mediates MAPK Pathway Activation in GNAQ Mutant Uveal Melanoma. Cancer Cell. 2017;31:685-696.e6 pubmed publisher
  5. Zhou Y, Chen S, Liu D, Manyande A, Zhang W, Yang S, et al. The Role of Spinal GABAB Receptors in Cancer-Induced Bone Pain in Rats. J Pain. 2017;18:933-946 pubmed publisher
  6. Weigand I, Ronchi C, Rizk Rabin M, Dalmazi G, Wild V, Bathon K, et al. Differential expression of the protein kinase A subunits in normal adrenal glands and adrenocortical adenomas. Sci Rep. 2017;7:49 pubmed publisher
  7. Salomon J, Gaston C, Magescas J, Duvauchelle B, Canioni D, Sengmanivong L, et al. Contractile forces at tricellular contacts modulate epithelial organization and monolayer integrity. Nat Commun. 2017;8:13998 pubmed publisher
  8. Eccles R, Czajkowski M, Barth C, Müller P, McShane E, Grunwald S, et al. Bimodal antagonism of PKA signalling by ARHGAP36. Nat Commun. 2016;7:12963 pubmed publisher
  9. Whiting J, Ogier L, Forbush K, Bucko P, Gopalan J, Seternes O, et al. AKAP220 manages apical actin networks that coordinate aquaporin-2 location and renal water reabsorption. Proc Natl Acad Sci U S A. 2016;113:E4328-37 pubmed publisher
  10. Bachmann V, Mayrhofer J, Ilouz R, Tschaikner P, Raffeiner P, Röck R, et al. Gpr161 anchoring of PKA consolidates GPCR and cAMP signaling. Proc Natl Acad Sci U S A. 2016;113:7786-91 pubmed publisher
  11. Phillips E, Lang V, Bohlen J, Bethke F, Puccio L, Tichy D, et al. Targeting atypical protein kinase C iota reduces viability in glioblastoma stem-like cells via a notch signaling mechanism. Int J Cancer. 2016;139:1776-87 pubmed publisher
  12. Hattori K, Naguro I, Okabe K, Funatsu T, Furutani S, Takeda K, et al. ASK1 signalling regulates brown and beige adipocyte function. Nat Commun. 2016;7:11158 pubmed publisher
  13. Riggle K, Riehle K, Kenerson H, Turnham R, Homma M, Kazami M, et al. Enhanced cAMP-stimulated protein kinase A activity in human fibrolamellar hepatocellular carcinoma. Pediatr Res. 2016;80:110-8 pubmed publisher
  14. Kamiyama D, Sekine S, Barsi Rhyne B, Hu J, Chen B, Gilbert L, et al. Versatile protein tagging in cells with split fluorescent protein. Nat Commun. 2016;7:11046 pubmed publisher
  15. Farini A, Sitzia C, Cassinelli L, Colleoni F, Parolini D, Giovanella U, et al. Inositol 1,4,5-trisphosphate (IP3)-dependent Ca2+ signaling mediates delayed myogenesis in Duchenne muscular dystrophy fetal muscle. Development. 2016;143:658-69 pubmed publisher
  16. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  17. Gerbaud P, Taskén K, Pidoux G. Spatiotemporal regulation of cAMP signaling controls the human trophoblast fusion. Front Pharmacol. 2015;6:202 pubmed publisher
  18. House C, Wang B, Ceniccola K, Williams R, Simaan M, Olender J, et al. Voltage-gated Na+ Channel Activity Increases Colon Cancer Transcriptional Activity and Invasion Via Persistent MAPK Signaling. Sci Rep. 2015;5:11541 pubmed publisher
  19. Rajan S, Dickson L, Mathew E, Orr C, Ellenbroek J, Philipson L, et al. Chronic hyperglycemia downregulates GLP-1 receptor signaling in pancreatic β-cells via protein kinase A. Mol Metab. 2015;4:265-76 pubmed publisher
  20. Lugenbiel P, Wenz F, Govorov K, Schweizer P, Katus H, Thomas D. Atrial fibrillation complicated by heart failure induces distinct remodeling of calcium cycling proteins. PLoS ONE. 2015;10:e0116395 pubmed publisher
  21. Grego Bessa J, Hildebrand J, Anderson K. Morphogenesis of the mouse neural plate depends on distinct roles of cofilin 1 in apical and basal epithelial domains. Development. 2015;142:1305-14 pubmed publisher
  22. Ahmad F, Shen W, Vandeput F, Szabo Fresnais N, Krall J, Degerman E, et al. Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2. J Biol Chem. 2015;290:6763-76 pubmed publisher
  23. Calebiro D, Hannawacker A, Lyga S, Bathon K, Zabel U, Ronchi C, et al. PKA catalytic subunit mutations in adrenocortical Cushing's adenoma impair association with the regulatory subunit. Nat Commun. 2014;5:5680 pubmed publisher
  24. Tse D, Chung I, Wu S. Pharmacological inhibitions of glutamate transporters EAAT1 and EAAT2 compromise glutamate transport in photoreceptor to ON-bipolar cell synapses. Vision Res. 2014;103:49-62 pubmed publisher
  25. Batsaikhan B, Yoshikawa K, Kurita N, Iwata T, Takasu C, Kashihara H, et al. Expression of Stathmin1 in gastric adenocarcinoma. Anticancer Res. 2014;34:4217-21 pubmed
  26. Tse D, Chung I, Wu S. Possible roles of glutamate transporter EAAT5 in mouse cone depolarizing bipolar cell light responses. Vision Res. 2014;103:63-74 pubmed publisher
  27. Moody S, Schinzel A, Singh S, Izzo F, Strickland M, Luo L, et al. PRKACA mediates resistance to HER2-targeted therapy in breast cancer cells and restores anti-apoptotic signaling. Oncogene. 2015;34:2061-71 pubmed publisher
  28. Batsaikhan B, Kurita N, Iwata T, Sato H, Yoshikawa K, Takasu C, et al. The role of activation-induced cytidine deaminase expression in gastric adenocarcinoma. Anticancer Res. 2014;34:995-1000 pubmed
  29. Yamanaka T, Tosaki A, Kurosawa M, Akimoto K, Hirose T, Ohno S, et al. Loss of aPKC? in differentiated neurons disrupts the polarity complex but does not induce obvious neuronal loss or disorientation in mouse brains. PLoS ONE. 2013;8:e84036 pubmed publisher
  30. Linch M, Sanz Garcia M, Rosse C, Riou P, Peel N, Madsen C, et al. Regulation of polarized morphogenesis by protein kinase C iota in oncogenic epithelial spheroids. Carcinogenesis. 2014;35:396-406 pubmed publisher
  31. Martin S, Fernandez Rojo M, Stanley A, Bastiani M, Okano S, Nixon S, et al. Caveolin-1 deficiency leads to increased susceptibility to cell death and fibrosis in white adipose tissue: characterization of a lipodystrophic model. PLoS ONE. 2012;7:e46242 pubmed publisher
  32. Blom J, Giove T, Deshpande M, Eldred W. Characterization of nitric oxide signaling pathways in the mouse retina. J Comp Neurol. 2012;520:4204-17 pubmed publisher
  33. Phillips M, Otteson D, Sherry D. Progression of neuronal and synaptic remodeling in the rd10 mouse model of retinitis pigmentosa. J Comp Neurol. 2010;518:2071-89 pubmed publisher
  34. Besalduch N, Tomas M, Santafe M, Garcia N, Tomas J, Lanuza M. Synaptic activity-related classical protein kinase C isoform localization in the adult rat neuromuscular synapse. J Comp Neurol. 2010;518:211-28 pubmed publisher
  35. Sugiyama Y, Akimoto K, Robinson M, Ohno S, Quinlan R. A cell polarity protein aPKClambda is required for eye lens formation and growth. Dev Biol. 2009;336:246-56 pubmed publisher
  36. Kishikawa M, Suzuki A, Ohno S. aPKC enables development of zonula adherens by antagonizing centripetal contraction of the circumferential actomyosin cables. J Cell Sci. 2008;121:2481-92 pubmed publisher
  37. Imai F, Hirai S, Akimoto K, Koyama H, Miyata T, Ogawa M, et al. Inactivation of aPKClambda results in the loss of adherens junctions in neuroepithelial cells without affecting neurogenesis in mouse neocortex. Development. 2006;133:1735-44 pubmed