This is a Validated Antibody Database (VAD) review about dog PPP1R12A, based on 18 published articles (read how Labome selects the articles), using PPP1R12A antibody in all methods. It is aimed to help Labome visitors find the most suited PPP1R12A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PPP1R12A synonym: protein phosphatase 1 regulatory subunit 12A; protein phosphatase 1, regulatory (inhibitor) subunit 12A

Cell Signaling Technology
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 7a
Cell Signaling Technology PPP1R12A antibody (Cell Signaling, 2634S) was used in western blot on human samples at 1:1000 (fig 7a). PLoS ONE (2018) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig s7a
In order to elucidate the role of IP3R in regulating blood pressure in vivo, Cell Signaling Technology PPP1R12A antibody (Cell signaling, 2634P) was used in western blot on mouse samples (fig s7a). JCI Insight (2016) ncbi
rabbit polyclonal
  • western blot; human; loading ...
In order to report the O-linked N-acetylglucosamine glycoproteome observed in human T cells, Cell Signaling Technology PPP1R12A antibody (Cell Signaling, 2634) was used in western blot on human samples . J Immunol (2016) ncbi
rabbit polyclonal
  • western blot; human
In order to report that inhibition of eIF5A reduces pancreatic ductal adenocarcinoma cell migration, invasion, and metastasis in vitro and in vivo, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, #2634) was used in western blot on human samples . J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; human
In order to report that inhibition of eIF5A reduces pancreatic ductal adenocarcinoma cell migration, invasion, and metastasis in vitro and in vivo, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, #4563) was used in western blot on human samples . J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig 5f
Cell Signaling Technology PPP1R12A antibody (Cell Signalling, 4563) was used in western blot on human samples at 1:500 (fig 5f). Nat Commun (2015) ncbi
rabbit monoclonal (D6C1)
  • western blot; human; 1:1000; loading ...; fig 5f
Cell Signaling Technology PPP1R12A antibody (Cell Signalling, 8574) was used in western blot on human samples at 1:1000 (fig 5f). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 2634) was used in western blot on human samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human
Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 4563) was used in western blot on human samples . PLoS ONE (2015) ncbi
rabbit monoclonal (D6C1)
  • western blot; human
In order to evaluate the effect of tumor necrosis factor on claudin-5 endothelial tight junction barriers, Cell Signaling Technology PPP1R12A antibody (Cell Signaling, 8574) was used in western blot on human samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 5
Cell Signaling Technology PPP1R12A antibody (Cell Signaling, 4563) was used in western blot on human samples (fig 5). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to study the effects of compressive stress on cellular functions, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 2634) was used in western blot on mouse samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to study the effects of compressive stress on cellular functions, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 4563) was used in western blot on mouse samples . PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; human
In order to explore the role of Rnd3 in non-small cell lung cancer, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 4563 S) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; human
In order to explore the role of Rnd3 in non-small cell lung cancer, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 2634 S) was used in western blot on human samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse
In order to study heart failure in Rnd3(+/-) haploinsufficient mice, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 2634S) was used in western blot on mouse samples . Cell Death Dis (2014) ncbi
rabbit polyclonal
  • western blot; mouse
In order to study heart failure in Rnd3(+/-) haploinsufficient mice, Cell Signaling Technology PPP1R12A antibody (Cell Signaling Technology, 4563S) was used in western blot on mouse samples . Cell Death Dis (2014) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 2
Cell Signaling Technology PPP1R12A antibody (Cell Signaling, 2634) was used in western blot on human samples at 1:1000 (fig 2). Nat Commun (2013) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; human; fig s3a
In order to find compounds that are synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma, EMD Millipore PPP1R12A antibody (Millipore, ABS45) was used in western blot on human samples (fig s3a). Oncogene (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; fig 2
In order to propose that epithelial tension and matricellular fibrosis contribute to the aggressiveness of SMAD4 mutant pancreatic tumors, EMD Millipore PPP1R12A antibody (Millipore, ABS45) was used in immunohistochemistry on mouse samples at 1:200 (fig 2). Nat Med (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:3500
EMD Millipore PPP1R12A antibody (Millipore, ABS45) was used in western blot on rat samples at 1:3500. J Biol Chem (2015) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4
In order to study phosphorylation events at sites of adhesion, EMD Millipore PPP1R12A antibody (Millipore, ABS45) was used in western blot on mouse samples at 1:1000 (fig 4). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to investigate the role of ROCK1 and ROCK2 in cell detachment, EMD Millipore PPP1R12A antibody (Millipore, ABS45) was used in western blot on mouse samples . Cell Death Dis (2013) ncbi
rabbit polyclonal
  • western blot; human
EMD Millipore PPP1R12A antibody (Millipore, ABS45) was used in western blot on human samples . Invest Ophthalmol Vis Sci (2013) ncbi
Articles Reviewed
  1. Yasuda Yamahara M, Rogg M, Yamahara K, Maier J, Huber T, Schell C. AIF1L regulates actomyosin contractility and filopodial extensions in human podocytes. PLoS ONE. 2018;13:e0200487 pubmed publisher
  2. Thompson J, Nguyen Q, Singh M, Pavesic M, Nesterenko I, Nelson L, et al. Rho-associated kinase 1 inhibition is synthetically lethal with von Hippel-Lindau deficiency in clear cell renal cell carcinoma. Oncogene. 2017;36:1080-1089 pubmed publisher
  3. Lin Q, Zhao G, Fang X, Peng X, Tang H, Wang H, et al. IP3 receptors regulate vascular smooth muscle contractility and hypertension. JCI Insight. 2016;1:e89402 pubmed
  4. Lund P, Elias J, Davis M. Global Analysis of O-GlcNAc Glycoproteins in Activated Human T Cells. J Immunol. 2016;197:3086-3098 pubmed
  5. Laklai H, Miroshnikova Y, Pickup M, Collisson E, Kim G, Barrett A, et al. Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression. Nat Med. 2016;22:497-505 pubmed publisher
  6. Fujimura K, Choi S, Wyse M, Strnadel J, Wright T, Klemke R. Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels. J Biol Chem. 2015;290:29907-19 pubmed publisher
  7. Caporali A, Meloni M, Nailor A, Mitić T, Shantikumar S, Riu F, et al. p75(NTR)-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia. Nat Commun. 2015;6:8024 pubmed publisher
  8. Yamana S, Tokiyama A, Mizutani K, Hirata K, Takai Y, Rikitake Y. The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation. PLoS ONE. 2015;10:e0124259 pubmed publisher
  9. Clark P, Kim R, Pober J, Kluger M. Tumor necrosis factor disrupts claudin-5 endothelial tight junction barriers in two distinct NF-κB-dependent phases. PLoS ONE. 2015;10:e0120075 pubmed publisher
  10. Giehl K, Keller C, Muehlich S, Goppelt Struebe M. Actin-mediated gene expression depends on RhoA and Rac1 signaling in proximal tubular epithelial cells. PLoS ONE. 2015;10:e0121589 pubmed publisher
  11. Takemoto K, Ishihara S, Mizutani T, Kawabata K, Haga H. Compressive stress induces dephosphorylation of the myosin regulatory light chain via RhoA phosphorylation by the adenylyl cyclase/protein kinase A signaling pathway. PLoS ONE. 2015;10:e0117937 pubmed publisher
  12. Mills R, Mita M, Nakagawa J, Shoji M, Sutherland C, Walsh M. A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle. J Biol Chem. 2015;290:8677-92 pubmed publisher
  13. Robertson J, Jacquemet G, Byron A, Jones M, Warwood S, Selley J, et al. Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun. 2015;6:6265 pubmed publisher
  14. Tang Y, Hu C, Yang H, Cao L, Li Y, Deng P, et al. Rnd3 regulates lung cancer cell proliferation through notch signaling. PLoS ONE. 2014;9:e111897 pubmed publisher
  15. Yue X, Yang X, Lin X, Yang T, Yi X, Dai Y, et al. Rnd3 haploinsufficient mice are predisposed to hemodynamic stress and develop apoptotic cardiomyopathy with heart failure. Cell Death Dis. 2014;5:e1284 pubmed publisher
  16. Gurkar A, Chu K, Raj L, Bouley R, Lee S, Kim Y, et al. Identification of ROCK1 kinase as a critical regulator of Beclin1-mediated autophagy during metabolic stress. Nat Commun. 2013;4:2189 pubmed publisher
  17. Shi J, Wu X, Surma M, Vemula S, Zhang L, Yang Y, et al. Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment. Cell Death Dis. 2013;4:e483 pubmed publisher
  18. Pattabiraman P, Pecen P, Rao P. MRP4-mediated regulation of intracellular cAMP and cGMP levels in trabecular meshwork cells and homeostasis of intraocular pressure. Invest Ophthalmol Vis Sci. 2013;54:1636-49 pubmed publisher