This is a Validated Antibody Database (VAD) review about dog PARD3, based on 25 published articles (read how Labome selects the articles), using PARD3 antibody in all methods. It is aimed to help Labome visitors find the most suited PARD3 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PARD3 synonym: PAR-3; partitioning defective 3 homolog; par-3 partitioning defective 3 homolog

EMD Millipore
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 4a
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in western blot on human samples at 1:1000 (fig 4a). elife (2019) ncbi
rabbit polyclonal
  • western blot; human; fig 2a
EMD Millipore PARD3 antibody (Millipore, 07330) was used in western blot on human samples (fig 2a). Proc Natl Acad Sci U S A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig 1i
  • western blot; mouse; loading ...; fig 1e
In order to describe how epithelial polarity proteins suppress malignant outgrowth, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry on mouse samples (fig 1i) and in western blot on mouse samples (fig 1e). J Exp Med (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:400; loading ...; fig 5d
In order to determine that Light Intermediate Chain 2-dynein is a major mediator of proper spindle orientation and uncover its underlying molecular mechanism, EMD Millipore PARD3 antibody (Milllipore, 07-330) was used in western blot on human samples at 1:400 (fig 5d). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...
In order to show that Endoglycan is a pre-luminal marker, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Dev Biol (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; loading ...; fig s1f
In order to use transgenic mice to clarify the role of LKB1 in the maintenance of functional tight junction, EMD Millipore PARD3 antibody (Milipore, 07-330) was used in immunohistochemistry on mouse samples (fig s1f). Hepatology (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; fig s2d
In order to explore how the interaction between beta1-integrin and Fgf2 contributes to the satellite cell niche, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunocytochemistry on mouse samples (fig s2d). Nat Med (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:500; fig 5
In order to report that crumbs homolog 3 is an actin microfilament regulator, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in western blot on rat samples at 1:500 (fig 5). Sci Rep (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:50; fig 7
  • western blot; mouse; 1:500; fig 6
In order to analyze preservation of epithelial polarity and epithelial barrier by alix-mediated assembly of the actomyosin-tight junction polarity complex, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig 7) and in western blot on mouse samples at 1:500 (fig 6). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; dog; fig s5
In order to analyze the relationship between Rab35 GTPase cell division and initiation of epithelial apico-basal polarity and lumen opening, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunocytochemistry on dog samples (fig s5). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry; chicken; 1:500; fig 4
In order to determine regulation of neural tube closure by modulating tight junctions by cell-cycle-dependent TGFbeta-BMP antagonism, EMD Millipore PARD3 antibody (Upstate, 07-330) was used in immunohistochemistry on chicken samples at 1:500 (fig 4). J Cell Sci (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:250; fig 5
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry on mouse samples at 1:250 (fig 5). J Cell Sci (2016) ncbi
rabbit polyclonal
EMD Millipore PARD3 antibody (Millipore, 07330) was used . J Biol Chem (2016) ncbi
rabbit polyclonal
  • proximity ligation assay; mouse; fig 3
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in proximity ligation assay on mouse samples (fig 3). Nat Med (2015) ncbi
rabbit polyclonal
  • western blot; rat; 1:500; fig 2g
In order to identify novel molecules in peripheral myelination by spatial mapping of juxtacrine axo-glial interactions, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in western blot on rat samples at 1:500 (fig 2g). Nat Commun (2015) ncbi
rabbit polyclonal
  • western blot; mouse
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in western blot on mouse samples . Cell Cycle (2014) ncbi
rabbit polyclonal
  • western blot; human
In order to investigate the localization of POF1B in desmosomes and its role in human intestinal and keratinocyte cell lines, EMD Millipore PARD3 antibody (Upstate, 07-330) was used in western blot on human samples . J Invest Dermatol (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:200
In order to investigate the relationships among abnormal spindle orientations, ectopic progenitors, and severe heterotopia in mouse and human, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry - frozen section on mouse samples at 1:200. Nat Neurosci (2014) ncbi
rabbit polyclonal
  • immunocytochemistry; human
  • western blot; human
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunocytochemistry on human samples and in western blot on human samples . Mol Oncol (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig 2
EMD Millipore PARD3 antibody (Upstate, 07-330) was used in western blot on mouse samples (fig 2). PLoS ONE (2013) ncbi
rabbit polyclonal
  • immunocytochemistry; dog; 1:200
In order to study the role of Kif3a in microtubular dynamics, migration and lumen formation of MDCK cells, EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunocytochemistry on dog samples at 1:200. PLoS ONE (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; fig s5
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry - paraffin section on mouse samples (fig s5). PLoS Genet (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:100
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry - paraffin section on mouse samples at 1:100. Development (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:100
EMD Millipore PARD3 antibody (Millipore, 07-330) was used in immunohistochemistry - paraffin section on human samples at 1:100. Int J Mol Sci (2013) ncbi
rabbit polyclonal
  • immunoprecipitation; mouse; 1:500; loading ...; fig 4c
  • immunocytochemistry; mouse; 1:100; fig s5
  • western blot; mouse; 1:500; loading ...; fig 4c
In order to investigate the role of Merlin in adheren junction establishment, EMD Millipore PARD3 antibody (Upstate, 07-330) was used in immunoprecipitation on mouse samples at 1:500 (fig 4c), in immunocytochemistry on mouse samples at 1:100 (fig s5) and in western blot on mouse samples at 1:500 (fig 4c). Dev Cell (2010) ncbi
Articles Reviewed
  1. Fearnley G, Young K, Edgar J, Antrobus R, Hay I, Liang W, et al. The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion. elife. 2019;8: pubmed publisher
  2. Hu L, Liang S, Chen H, Lv T, Wu J, Chen D, et al. ΔNp63α is a common inhibitory target in oncogenic PI3K/Ras/Her2-induced cell motility and tumor metastasis. Proc Natl Acad Sci U S A. 2017;114:E3964-E3973 pubmed publisher
  3. Mescher M, Jeong P, Knapp S, Rübsam M, Saynisch M, Kranen M, et al. The epidermal polarity protein Par3 is a non-cell autonomous suppressor of malignant melanoma. J Exp Med. 2017;214:339-358 pubmed publisher
  4. Mahale S, Kumar M, Sharma A, Babu A, Ranjan S, Sachidanandan C, et al. The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation. Sci Rep. 2016;6:22 pubmed publisher
  5. Yang Z, Zimmerman S, Tsunezumi J, Braitsch C, Trent C, Bryant D, et al. Role of CD34 family members in lumen formation in the developing kidney. Dev Biol. 2016;418:66-74 pubmed publisher
  6. Porat Shliom N, Tietgens A, Van Itallie C, Vitale Cross L, Jarnik M, Harding O, et al. Liver kinase B1 regulates hepatocellular tight junction distribution and function in vivo. Hepatology. 2016;64:1317-29 pubmed publisher
  7. Rozo M, Li L, Fan C. Targeting ?1-integrin signaling enhances regeneration in aged and dystrophic muscle in mice. Nat Med. 2016;22:889-96 pubmed publisher
  8. Gao Y, Lui W, Lee W, Cheng C. Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells. Sci Rep. 2016;6:28589 pubmed publisher
  9. Campos Y, Qiu X, Gomero E, Wakefield R, Horner L, Brutkowski W, et al. Alix-mediated assembly of the actomyosin-tight junction polarity complex preserves epithelial polarity and epithelial barrier. Nat Commun. 2016;7:11876 pubmed publisher
  10. Klinkert K, Rocancourt M, Houdusse A, Echard A. Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening. Nat Commun. 2016;7:11166 pubmed publisher
  11. Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
  12. Jimeno D, Gómez C, Calzada N, de la Villa P, Lillo C, Santos E. RASGRF2 controls nuclear migration in postnatal retinal cone photoreceptors. J Cell Sci. 2016;129:729-42 pubmed publisher
  13. Chow C, Ebine K, Knab L, Bentrem D, Kumar K, Munshi H. Cancer Cell Invasion in Three-dimensional Collagen Is Regulated Differentially by Gα13 Protein and Discoidin Domain Receptor 1-Par3 Protein Signaling. J Biol Chem. 2016;291:1605-18 pubmed publisher
  14. Dumont N, Wang Y, von Maltzahn J, Pasut A, Bentzinger C, Brun C, et al. Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division. Nat Med. 2015;21:1455-63 pubmed publisher
  15. Poitelon Y, Bogni S, Matafora V, Della Flora Nunes G, Hurley E, Ghidinelli M, et al. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun. 2015;6:8303 pubmed publisher
  16. Hoon J, Li H, Koh C. POPX2 phosphatase regulates cell polarity and centrosome placement. Cell Cycle. 2014;13:2459-68 pubmed publisher
  17. Crespi A, Bertoni A, Ferrari I, Padovano V, Della Mina P, Berti E, et al. POF1B localizes to desmosomes and regulates cell adhesion in human intestinal and keratinocyte cell lines. J Invest Dermatol. 2015;135:192-201 pubmed publisher
  18. Kielar M, Tuy F, Bizzotto S, Lebrand C, de Juan Romero C, Poirier K, et al. Mutations in Eml1 lead to ectopic progenitors and neuronal heterotopia in mouse and human. Nat Neurosci. 2014;17:923-33 pubmed publisher
  19. Facciuto F, Bugnon Valdano M, Marziali F, Massimi P, Banks L, Cavatorta A, et al. Human papillomavirus (HPV)-18 E6 oncoprotein interferes with the epithelial cell polarity Par3 protein. Mol Oncol. 2014;8:533-43 pubmed publisher
  20. Yamanaka T, Tosaki A, Kurosawa M, Akimoto K, Hirose T, Ohno S, et al. Loss of aPKC? in differentiated neurons disrupts the polarity complex but does not induce obvious neuronal loss or disorientation in mouse brains. PLoS ONE. 2013;8:e84036 pubmed publisher
  21. Boehlke C, Kotsis F, Buchholz B, Powelske C, Eckardt K, Walz G, et al. Kif3a guides microtubular dynamics, migration and lumen formation of MDCK cells. PLoS ONE. 2013;8:e62165 pubmed publisher
  22. Reginensi A, Scott R, Gregorieff A, Bagherie Lachidan M, Chung C, Lim D, et al. Yap- and Cdc42-dependent nephrogenesis and morphogenesis during mouse kidney development. PLoS Genet. 2013;9:e1003380 pubmed publisher
  23. Yang Z, Zimmerman S, BRAKEMAN P, Beaudoin G, Reichardt L, MARCIANO D. De novo lumen formation and elongation in the developing nephron: a central role for afadin in apical polarity. Development. 2013;140:1774-84 pubmed publisher
  24. Jan Y, Ko B, Liu T, Wu Y, Liang S, Chen S, et al. Expression of partitioning defective 3 (Par-3) for predicting extrahepatic metastasis and survival with hepatocellular carcinoma. Int J Mol Sci. 2013;14:1684-97 pubmed publisher
  25. Gladden A, Hebert A, Schneeberger E, McClatchey A. The NF2 tumor suppressor, Merlin, regulates epidermal development through the establishment of a junctional polarity complex. Dev Cell. 2010;19:727-39 pubmed publisher