This is a Validated Antibody Database (VAD) review about dog HSPB1, based on 22 published articles (read how Labome selects the articles), using HSPB1 antibody in all methods. It is aimed to help Labome visitors find the most suited HSPB1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HSPB1 synonym: HSP27; heat shock protein beta-1; heat shock protein 27 kDa beta-1

Enzo Life Sciences
rabbit polyclonal
  • immunohistochemistry; rat; 1:500; loading ...; fig 1c
  • western blot; rat; 1:500; loading ...; fig 4d
In order to examine if heat shock protein 25 induces astroglial apoptosis or clasmatodendrosis in status epilepticus-induced rat hippocampi, Enzo Life Sciences HSPB1 antibody (Enzo, ADI-SPA-801) was used in immunohistochemistry on rat samples at 1:500 (fig 1c) and in western blot on rat samples at 1:500 (fig 4d). Front Cell Neurosci (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:500; loading ...; fig 1c
  • western blot; rat; 1:500; loading ...; fig 4d
In order to examine if heat shock protein 25 induces astroglial apoptosis or clasmatodendrosis in status epilepticus-induced rat hippocampi, Enzo Life Sciences HSPB1 antibody (Enzo, ADI-SPA-801) was used in immunohistochemistry on rat samples at 1:500 (fig 1c) and in western blot on rat samples at 1:500 (fig 4d). Front Cell Neurosci (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; rat; 1:500; loading ...; fig 1c
  • western blot; rat; 1:500; loading ...; fig 4d
In order to examine if heat shock protein 25 induces astroglial apoptosis or clasmatodendrosis in status epilepticus-induced rat hippocampi, Enzo Life Sciences HSPB1 antibody (Enzo, ADI-SPA-801) was used in immunohistochemistry on rat samples at 1:500 (fig 1c) and in western blot on rat samples at 1:500 (fig 4d). Front Cell Neurosci (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 1e
  • western blot; human; loading ...; fig 1e
Enzo Life Sciences HSPB1 antibody (Enzo, ADI-SPA-801-F) was used in western blot on mouse samples (fig 1e) and in western blot on human samples (fig 1e). Nat Commun (2017) ncbi
rabbit polyclonal
  • western blot; mouse; loading ...; fig 6g
In order to research the roles of HSF1 and mTORC1 in proteotoxic stress and cell growth, Enzo Life Sciences HSPB1 antibody (Enzo, ADI-SPA-801) was used in western blot on mouse samples (fig 6g). Nat Cell Biol (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Enzo Life Sciences HSPB1 antibody (Enzo life sciences, ADI-SPA-801) was used in western blot on mouse samples (fig 5). Mol Metab (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Enzo Life Sciences HSPB1 antibody (Enzo life sciences, ADI-SPA-801) was used in western blot on mouse samples (fig 5). Mol Metab (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Enzo Life Sciences HSPB1 antibody (Enzo life sciences, ADI-SPA-801) was used in western blot on mouse samples (fig 5). Mol Metab (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Enzo Life Sciences HSPB1 antibody (Enzo life sciences, ADI-SPA-801) was used in western blot on mouse samples (fig 5). Mol Metab (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Enzo Life Sciences HSPB1 antibody (Enzo life sciences, ADI-SPA-801) was used in western blot on mouse samples (fig 5). Mol Metab (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Enzo Life Sciences HSPB1 antibody (Enzo life sciences, ADI-SPA-801) was used in western blot on mouse samples (fig 5). Mol Metab (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
Enzo Life Sciences HSPB1 antibody (Enzo life sciences, ADI-SPA-801) was used in western blot on mouse samples (fig 5). Mol Metab (2016) ncbi
rabbit polyclonal
  • western blot; human; fig s1
Enzo Life Sciences HSPB1 antibody (Enzo, ADI-SPA-525) was used in western blot on human samples (fig s1). Cell Death Dis (2015) ncbi
rabbit polyclonal
  • western blot; mouse
In order to determine if HSPs ameliorate ischemic renal injury in vivo using HSF-1 KO mice, Enzo Life Sciences HSPB1 antibody (Stressgen, SPA 801) was used in western blot on mouse samples . Kidney Int (2014) ncbi
rabbit polyclonal
  • western blot; mouse
In order to determine if HSPs ameliorate ischemic renal injury in vivo using HSF-1 KO mice, Enzo Life Sciences HSPB1 antibody (Stressgen, SPA 801) was used in western blot on mouse samples . Kidney Int (2014) ncbi
rabbit polyclonal
  • western blot; mouse
In order to determine if HSPs ameliorate ischemic renal injury in vivo using HSF-1 KO mice, Enzo Life Sciences HSPB1 antibody (Stressgen, SPA 801) was used in western blot on mouse samples . Kidney Int (2014) ncbi
rabbit polyclonal
  • western blot; mouse
Enzo Life Sciences HSPB1 antibody (Enzo Life Sciences, ADI-SPA-801) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse
Enzo Life Sciences HSPB1 antibody (Enzo Life Sciences, ADI-SPA-801) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse
Enzo Life Sciences HSPB1 antibody (Enzo Life Sciences, ADI-SPA-801) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 8
In order to demonstrate that HACE1 protects the heart under pressure stress by regulating protein degradation, Enzo Life Sciences HSPB1 antibody (Stressgen, SPA801) was used in western blot on mouse samples at 1:1000 (fig 8). Nat Commun (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 8
In order to demonstrate that HACE1 protects the heart under pressure stress by regulating protein degradation, Enzo Life Sciences HSPB1 antibody (Stressgen, SPA801) was used in western blot on mouse samples at 1:1000 (fig 8). Nat Commun (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 8
In order to demonstrate that HACE1 protects the heart under pressure stress by regulating protein degradation, Enzo Life Sciences HSPB1 antibody (Stressgen, SPA801) was used in western blot on mouse samples at 1:1000 (fig 8). Nat Commun (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000
Enzo Life Sciences HSPB1 antibody (Stressgen, ADI-SPA 801) was used in western blot on rat samples at 1:2000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000
Enzo Life Sciences HSPB1 antibody (Stressgen, ADI-SPA 801) was used in western blot on rat samples at 1:2000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; rat; 1:2000
Enzo Life Sciences HSPB1 antibody (Stressgen, ADI-SPA 801) was used in western blot on rat samples at 1:2000. PLoS ONE (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1, 2
In order to demonstrate that p38MAPK activation elevates mitochondrial ROS levels, Enzo Life Sciences HSPB1 antibody (Enzo Life Sciences, SPA-801) was used in western blot on mouse samples (fig 1, 2). Cell Commun Signal (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1, 2
In order to demonstrate that p38MAPK activation elevates mitochondrial ROS levels, Enzo Life Sciences HSPB1 antibody (Enzo Life Sciences, SPA-801) was used in western blot on mouse samples (fig 1, 2). Cell Commun Signal (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig 1, 2
In order to demonstrate that p38MAPK activation elevates mitochondrial ROS levels, Enzo Life Sciences HSPB1 antibody (Enzo Life Sciences, SPA-801) was used in western blot on mouse samples (fig 1, 2). Cell Commun Signal (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4
Enzo Life Sciences HSPB1 antibody (Enzo, SPA-801) was used in western blot on mouse samples at 1:1000 (fig 4). Cell Stress Chaperones (2012) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4
Enzo Life Sciences HSPB1 antibody (Enzo, SPA-801) was used in western blot on mouse samples at 1:1000 (fig 4). Cell Stress Chaperones (2012) ncbi
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 4
Enzo Life Sciences HSPB1 antibody (Enzo, SPA-801) was used in western blot on mouse samples at 1:1000 (fig 4). Cell Stress Chaperones (2012) ncbi
Abcam
mouse monoclonal (G3.1)
  • western blot; mouse; 1:5000; loading ...; fig 5b
Abcam HSPB1 antibody (Abcam, ab2790) was used in western blot on mouse samples at 1:5000 (fig 5b). Exp Neurol (2017) ncbi
mouse monoclonal (G3.1)
  • western blot; human; loading ...; fig 6b
Abcam HSPB1 antibody (Abcam, ab2790) was used in western blot on human samples (fig 6b). Sci Rep (2016) ncbi
mouse monoclonal (G3.1)
  • western blot; rat; fig 4
Abcam HSPB1 antibody (Abcam, ab2790) was used in western blot on rat samples (fig 4). Mol Brain (2016) ncbi
mouse monoclonal (G3.1)
  • western blot; pig; 1:1000; loading ...; fig 5b
Abcam HSPB1 antibody (Abcam, AB2790) was used in western blot on pig samples at 1:1000 (fig 5b). J Mol Endocrinol (2015) ncbi
mouse monoclonal (G3.1)
  • western blot; human; 1:2000; fig 3
Abcam HSPB1 antibody (Abcam, ab2790) was used in western blot on human samples at 1:2000 (fig 3). Aging Cell (2015) ncbi
mouse monoclonal (G3.1)
  • western blot; human; fig 1
In order to identify molecules that block Hsp70 activity on keratin filaments during inflammation, Abcam HSPB1 antibody (Abcam, ab2790) was used in western blot on human samples (fig 1). J Cell Sci (2014) ncbi
Invitrogen
mouse monoclonal (G3.1)
  • western blot; human; 1:1000
In order to determine if the type of contraction affects the localization or phosphorylation of sHSPs, HSP27, and alphaB-crystallin in human muscle, Invitrogen HSPB1 antibody (Thermo Scientific, MA3-015) was used in western blot on human samples at 1:1000. J Appl Physiol (1985) (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (D1H2)
  • immunohistochemistry; rat; 1:100; fig 6
In order to investigate VEGF-A antagonism due to distal retinal ganglion cell axon transport loss and activation of p38 MAPK stress pathway, Cell Signaling Technology HSPB1 antibody (Cell Signaling, 9709S) was used in immunohistochemistry on rat samples at 1:100 (fig 6). Cell Death Dis (2016) ncbi
rabbit monoclonal (D1H2)
  • western blot; human; 1:2500; fig 3
Cell Signaling Technology HSPB1 antibody (Cell Signaling, 9709) was used in western blot on human samples at 1:2500 (fig 3). Nat Commun (2015) ncbi
rabbit monoclonal (D1H2)
  • reverse phase protein lysate microarray; human; tbl s2
  • immunocytochemistry; human; 1:800
Cell Signaling Technology HSPB1 antibody (Cell Signaling Technology, 9709P) was used in reverse phase protein lysate microarray on human samples (tbl s2) and in immunocytochemistry on human samples at 1:800. Mol Syst Biol (2015) ncbi
rabbit monoclonal (D1H2)
  • western blot; human; fig s1
Cell Signaling Technology HSPB1 antibody (Cell Signaling, D1H2) was used in western blot on human samples (fig s1). Cell Death Dis (2015) ncbi
rabbit monoclonal (D1H2)
  • western blot; human; loading ...; fig 8a
Cell Signaling Technology HSPB1 antibody (Cell signaling, D1H2) was used in western blot on human samples (fig 8a). Eur J Pharmacol (2015) ncbi
Articles Reviewed
  1. Heilman P, Song S, Miranda C, Meyer K, Srivastava A, Knapp A, et al. HSPB1 mutations causing hereditary neuropathy in humans disrupt non-cell autonomous protection of motor neurons. Exp Neurol. 2017;297:101-109 pubmed publisher
  2. Kim J, Hyun H, Min S, Kang T. Sustained HSP25 Expression Induces Clasmatodendrosis via ER Stress in the Rat Hippocampus. Front Cell Neurosci. 2017;11:47 pubmed publisher
  3. Gómez Pastor R, Burchfiel E, Neef D, Jaeger A, Cabiscol E, McKinstry S, et al. Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease. Nat Commun. 2017;8:14405 pubmed publisher
  4. Hadadi E, Zhang B, Baidžajevas K, Yusof N, Puan K, Ong S, et al. Differential IL-1? secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability. Sci Rep. 2016;6:39035 pubmed publisher
  5. Zhai W, Chen D, Shen H, Chen Z, Li H, Yu Z, et al. A1 adenosine receptor attenuates intracerebral hemorrhage-induced secondary brain injury in rats by activating the P38-MAPKAP2-Hsp27 pathway. Mol Brain. 2016;9:66 pubmed publisher
  6. Foxton R, Osborne A, Martin K, Ng Y, Shima D. Distal retinal ganglion cell axon transport loss and activation of p38 MAPK stress pathway following VEGF-A antagonism. Cell Death Dis. 2016;7:e2212 pubmed publisher
  7. Su K, Cao J, Tang Z, Dai S, He Y, Sampson S, et al. HSF1 critically attunes proteotoxic stress sensing by mTORC1 to combat stress and promote growth. Nat Cell Biol. 2016;18:527-39 pubmed publisher
  8. Ost M, Coleman V, Voigt A, van Schothorst E, Keipert S, van der Stelt I, et al. Muscle mitochondrial stress adaptation operates independently of endogenous FGF21 action. Mol Metab. 2016;5:79-90 pubmed publisher
  9. Tollenaere M, Villumsen B, Blasius M, Nielsen J, Wagner S, Bartek J, et al. p38- and MK2-dependent signalling promotes stress-induced centriolar satellite remodelling via 14-3-3-dependent sequestration of CEP131/AZI1. Nat Commun. 2015;6:10075 pubmed publisher
  10. Cui Y, Gu X. Proteomic changes of the porcine small intestine in response to chronic heat stress. J Mol Endocrinol. 2015;55:277-93 pubmed publisher
  11. Fallahi Sichani M, Moerke N, Niepel M, Zhang T, Gray N, Sorger P. Systematic analysis of BRAF(V600E) melanomas reveals a role for JNK/c-Jun pathway in adaptive resistance to drug-induced apoptosis. Mol Syst Biol. 2015;11:797 pubmed publisher
  12. Morlé A, Garrido C, Micheau O. Hyperthermia restores apoptosis induced by death receptors through aggregation-induced c-FLIP cytosolic depletion. Cell Death Dis. 2015;6:e1633 pubmed publisher
  13. Nishi H, Maeda N, Izumi S, Higa Nakamine S, Toku S, Kakinohana M, et al. Differential regulation of epidermal growth factor receptor by hydrogen peroxide and flagellin in cultured lung alveolar epithelial cells. Eur J Pharmacol. 2015;748:133-42 pubmed publisher
  14. Gabriel D, Roedl D, Gordon L, Djabali K. Sulforaphane enhances progerin clearance in Hutchinson-Gilford progeria fibroblasts. Aging Cell. 2015;14:78-91 pubmed publisher
  15. Mashukova A, Kozhekbaeva Z, Forteza R, Dulam V, Figueroa Y, Warren R, et al. The BAG-1 isoform BAG-1M regulates keratin-associated Hsp70 chaperoning of aPKC in intestinal cells during activation of inflammatory signaling. J Cell Sci. 2014;127:3568-77 pubmed publisher
  16. Sreedharan R, Chen S, Miller M, Haribhai D, Williams C, Van Why S. Mice with an absent stress response are protected against ischemic renal injury. Kidney Int. 2014;86:515-24 pubmed publisher
  17. Ost M, Werner F, Dokas J, Klaus S, Voigt A. Activation of AMPK?2 is not crucial for mitochondrial uncoupling-induced metabolic effects but required to maintain skeletal muscle integrity. PLoS ONE. 2014;9:e94689 pubmed publisher
  18. Frankenberg N, Lamb G, Overgaard K, Murphy R, Vissing K. Small heat shock proteins translocate to the cytoskeleton in human skeletal muscle following eccentric exercise independently of phosphorylation. J Appl Physiol (1985). 2014;116:1463-72 pubmed publisher
  19. Zhang L, Chen X, Sharma P, Moon M, Sheftel A, Dawood F, et al. HACE1-dependent protein degradation provides cardiac protection in response to haemodynamic stress. Nat Commun. 2014;5:3430 pubmed publisher
  20. Moura C, Lollo P, Morato P, Nisishima L, Carneiro E, Amaya Farfan J. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS ONE. 2014;9:e83437 pubmed publisher
  21. Ashraf M, Ebner M, Wallner C, Haller M, Khalid S, Schwelberger H, et al. A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury. Cell Commun Signal. 2014;12:6 pubmed publisher
  22. Reina C, Nabet B, Young P, Pittman R. Basal and stress-induced Hsp70 are modulated by ataxin-3. Cell Stress Chaperones. 2012;17:729-42 pubmed publisher