This is a Validated Antibody Database (VAD) review about dog HSPA8, based on 26 published articles (read how Labome selects the articles), using HSPA8 antibody in all methods. It is aimed to help Labome visitors find the most suited HSPA8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HSPA8 synonym: heat shock cognate 71 kDa protein

Enzo Life Sciences
rat monoclonal (1B5)
  • western blot; mouse; loading ...; fig 1d
In order to study the role of hypercholesterolemia in T cell receptor signaling and regulatory T cell population, Enzo Life Sciences HSPA8 antibody (Assay designs, 1B5) was used in western blot on mouse samples (fig 1d). Sci Rep (2017) ncbi
rabbit polyclonal
  • western blot; dog; 1:1000; loading ...; fig 3
Enzo Life Sciences HSPA8 antibody (Enzo Life Sciences, ADI-SPA-757) was used in western blot on dog samples at 1:1000 (fig 3). Int J Hyperthermia (2016) ncbi
rat monoclonal (1B5)
  • western blot; human; fig 1
Enzo Life Sciences HSPA8 antibody (Enzo life science, ADI-SPA-815) was used in western blot on human samples (fig 1). PLoS ONE (2016) ncbi
rat monoclonal (1B5)
  • western blot; mouse; 1:5000; fig 1
In order to investigate breast tumorigenesis due to loss of Panx1 which impairs mammary gland development at lactation, Enzo Life Sciences HSPA8 antibody (Stressgen Bioreagents, SPA-815) was used in western blot on mouse samples at 1:5000 (fig 1). PLoS ONE (2016) ncbi
rat monoclonal (1B5)
  • immunohistochemistry - paraffin section; human; 1:300; fig 2
Enzo Life Sciences HSPA8 antibody (Stressgene, SPA815) was used in immunohistochemistry - paraffin section on human samples at 1:300 (fig 2). Oncol Lett (2016) ncbi
rat monoclonal (1B5)
  • western blot; human; fig s3
Enzo Life Sciences HSPA8 antibody (Enzo Life Sciences, 1B5) was used in western blot on human samples (fig s3). Proc Natl Acad Sci U S A (2016) ncbi
rat monoclonal (1B5)
  • western blot; mouse; fig 3
Enzo Life Sciences HSPA8 antibody (Enzo Life Sciences, ADI-SPA-815D) was used in western blot on mouse samples (fig 3). J Cell Biol (2015) ncbi
rat monoclonal (1B5)
  • western blot; mouse; 1:2000; fig 4
In order to report that K63 ubiquitination is required for chaperone-mediated autophagy degradation of HIF1A in vitro and in vivo, Enzo Life Sciences HSPA8 antibody (Stressgen, ADI-SPA-815) was used in western blot on mouse samples at 1:2000 (fig 4). Sci Rep (2015) ncbi
rat monoclonal (1B5)
  • western blot; human; 1:1000; fig 7
Enzo Life Sciences HSPA8 antibody (Stressgen, ADI-SPA-815) was used in western blot on human samples at 1:1000 (fig 7). PLoS Pathog (2015) ncbi
rat monoclonal (1B5)
  • western blot; Garra rufa
In order to use Garra rufa to study heat shock proteins in different oxidative stress and thermal conditions, Enzo Life Sciences HSPA8 antibody (StressGen, SPA-815) was used in western blot on Garra rufa samples . Redox Biol (2014) ncbi
rat monoclonal (1B5)
  • western blot; human
Enzo Life Sciences HSPA8 antibody (Stressgen Bioreagents, SPA-815) was used in western blot on human samples . J Biol Chem (2014) ncbi
rat monoclonal (1B5)
  • immunohistochemistry - paraffin section; human; 1:100
In order to investigate the association of eosinophilic neuronal cytoplasmic inclusions with stress granules and autophagy in two clinical cases, Enzo Life Sciences HSPA8 antibody (Stressgen BIOTECHNOLOGIES, 1B5) was used in immunohistochemistry - paraffin section on human samples at 1:100. Neuropathology (2014) ncbi
rat monoclonal (1B5)
  • immunocytochemistry; human
In order to study the biophysical properties of amyotrophic lateral sclerosis-linked mutations of Cu/Zn superoxide dismutase 1 in human cells, Enzo Life Sciences HSPA8 antibody (Stressgen, SPA815) was used in immunocytochemistry on human samples . Genes Cells (2014) ncbi
rat monoclonal (1B5)
  • western blot; mouse; 1:2500; fig 4
Enzo Life Sciences HSPA8 antibody (Enzo, SPA-815) was used in western blot on mouse samples at 1:2500 (fig 4). Cell Stress Chaperones (2012) ncbi
Abcam
rat monoclonal (1B5)
  • western blot; human; 1:1000; loading ...; fig 4d
Abcam HSPA8 antibody (Abcam, ab19136) was used in western blot on human samples at 1:1000 (fig 4d). J Cell Biol (2017) ncbi
rat monoclonal (1B5)
  • western blot; human; 1:1000; loading ...; fig 7B
In order to identify cellular processes that regulate HIF1alpha, Abcam HSPA8 antibody (Abcam, ab19136) was used in western blot on human samples at 1:1000 (fig 7B). elife (2017) ncbi
rat monoclonal (1B5)
  • western blot; human; loading ...; fig 3b
In order to explore the role of chaperone-mediated autophagy in non-small-cell lung cancer, Abcam HSPA8 antibody (Abcam, ab19136) was used in western blot on human samples (fig 3b). Biochem Biophys Res Commun (2017) ncbi
rat monoclonal (1B5)
  • western blot; human; fig s1
Abcam HSPA8 antibody (Abcam, ab19136) was used in western blot on human samples (fig s1). Nucleic Acids Res (2015) ncbi
rat monoclonal (1B5)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
In order to characterize prevention of apoptosis by degrading BBC3/PUMA due to chaperone-mediated autophagy, Abcam HSPA8 antibody (abcam, ab19136) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 1). Autophagy (2015) ncbi
rat monoclonal (1B5)
  • western blot; mouse; loading ...; fig s1a
Abcam HSPA8 antibody (Abcam, ab19136) was used in western blot on mouse samples (fig s1a). Nat Commun (2015) ncbi
rat monoclonal (1B5)
  • western blot; rat; fig 3
Abcam HSPA8 antibody (AbCam, ab19136) was used in western blot on rat samples (fig 3). J Neurochem (2015) ncbi
rat monoclonal (1B5)
  • western blot; rat; 1:10000
Abcam HSPA8 antibody (Abcam, ab19136) was used in western blot on rat samples at 1:10000. J Biol Chem (2014) ncbi
rat monoclonal (1B5)
  • immunocytochemistry; human
Abcam HSPA8 antibody (Abcam, ab 19136) was used in immunocytochemistry on human samples . Mol Oncol (2013) ncbi
Cell Signaling Technology
rabbit monoclonal (D12F2)
  • western blot; mouse; loading ...; fig s3b
Cell Signaling Technology HSPA8 antibody (Cell Signaling, 8444) was used in western blot on mouse samples (fig s3b). Cell Death Differ (2016) ncbi
rabbit monoclonal (D12F2)
  • western blot; mouse; 1:1000; fig 6a
In order to uncover a role for ATG12-ATG3 in late endosome function that is distinct from autophagy, Cell Signaling Technology HSPA8 antibody (Cell Signaling, 8444) was used in western blot on mouse samples at 1:1000 (fig 6a). Nat Cell Biol (2015) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; human
In order to study the roles of prostate tumor suppressor NKX3.1 in DNA repair and luminal cell differentiation, Sigma-Aldrich HSPA8 antibody (Sigma-Aldrich, SAB2101098) was used in western blot on human samples . F1000Res (2014) ncbi
Articles Reviewed
  1. Mailer R, Gisterå A, Polyzos K, Ketelhuth D, Hansson G. Hypercholesterolemia Enhances T Cell Receptor Signaling and Increases the Regulatory T Cell Population. Sci Rep. 2017;7:15655 pubmed publisher
  2. Ganguly A, Han X, Das U, Wang L, Loi J, Sun J, et al. Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin. J Cell Biol. 2017;216:2059-2074 pubmed publisher
  3. Miles A, Burr S, Grice G, Nathan J. The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1? prolyl hydroxylation by regulating cellular iron levels. elife. 2017;6: pubmed publisher
  4. Suzuki J, Nakajima W, Suzuki H, Asano Y, Tanaka N. Chaperone-mediated autophagy promotes lung cancer cell survival through selective stabilization of the pro-survival protein, MCL1. Biochem Biophys Res Commun. 2017;482:1334-1340 pubmed publisher
  5. Graner A, Hellwinkel J, Lencioni A, Madsen H, Harland T, Marchando P, et al. HSP90 inhibitors in the context of heat shock and the unfolded protein response: effects on a primary canine pulmonary adenocarcinoma cell line. Int J Hyperthermia. 2016;:1-15 pubmed publisher
  6. Dhamad A, Zhou Z, Zhou J, Du Y. Systematic Proteomic Identification of the Heat Shock Proteins (Hsp) that Interact with Estrogen Receptor Alpha (ER?) and Biochemical Characterization of the ER?-Hsp70 Interaction. PLoS ONE. 2016;11:e0160312 pubmed publisher
  7. Stewart M, Plante I, Penuela S, Laird D. Loss of Panx1 Impairs Mammary Gland Development at Lactation: Implications for Breast Tumorigenesis. PLoS ONE. 2016;11:e0154162 pubmed publisher
  8. Trieb K, Sulzbacher I, Kubista B. Recurrence rate and progression of chondrosarcoma is correlated with heat shock protein expression. Oncol Lett. 2016;11:521-524 pubmed
  9. Kowal J, Arras G, Colombo M, Jouve M, Morath J, Primdal Bengtson B, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016;113:E968-77 pubmed publisher
  10. Lisak D, Schacht T, Gawlitza A, Albrecht P, Aktas O, Koop B, et al. BAX inhibitor-1 is a Ca(2+) channel critically important for immune cell function and survival. Cell Death Differ. 2016;23:358-68 pubmed publisher
  11. Hyenne V, Apaydin A, Rodriguez D, Spiegelhalter C, Hoff Yoessle S, Diem M, et al. RAL-1 controls multivesicular body biogenesis and exosome secretion. J Cell Biol. 2015;211:27-37 pubmed publisher
  12. Rivera C, Saavedra F, Alvarez F, Díaz Celis C, Ugalde V, Li J, et al. Methylation of histone H3 lysine 9 occurs during translation. Nucleic Acids Res. 2015;43:9097-106 pubmed publisher
  13. Xie W, Zhang L, Jiao H, Guan L, Zha J, Li X, et al. Chaperone-mediated autophagy prevents apoptosis by degrading BBC3/PUMA. Autophagy. 2015;11:1623-35 pubmed publisher
  14. Ferreira J, Soares A, Ramalho J, Pereira P, Girao H. K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy. Sci Rep. 2015;5:10210 pubmed publisher
  15. Merkestein M, Laber S, McMurray F, Andrew D, Sachse G, Sanderson J, et al. FTO influences adipogenesis by regulating mitotic clonal expansion. Nat Commun. 2015;6:6792 pubmed publisher
  16. Tome M, Schaefer C, Jacobs L, Zhang Y, Herndon J, Matty F, et al. Identification of P-glycoprotein co-fractionating proteins and specific binding partners in rat brain microvessels. J Neurochem. 2015;134:200-10 pubmed publisher
  17. Honegger A, Schilling D, Bastian S, Sponagel J, Kuryshev V, Sultmann H, et al. Dependence of intracellular and exosomal microRNAs on viral E6/E7 oncogene expression in HPV-positive tumor cells. PLoS Pathog. 2015;11:e1004712 pubmed publisher
  18. Murrow L, Malhotra R, Debnath J. ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function. Nat Cell Biol. 2015;17:300-10 pubmed publisher
  19. Oksala N, Ekmekçi F, Ozsoy E, Kirankaya S, Kokkola T, Emecen G, et al. Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress. Redox Biol. 2014;3:25-8 pubmed publisher
  20. Yang C, Chung A, Ku C, Brill L, Williams R, Wolf D. Systems analysis of the prostate tumor suppressor NKX3.1 supports roles in DNA repair and luminal cell differentiation. F1000Res. 2014;3:115 pubmed publisher
  21. Suico M, Fukuda R, Miyakita R, Koyama K, Taura M, Shuto T, et al. The transcription factor MEF/Elf4 is dually modulated by p53-MDM2 axis and MEF-MDM2 autoregulatory mechanism. J Biol Chem. 2014;289:26143-54 pubmed publisher
  22. Mori F, Watanabe Y, Miki Y, Tanji K, Odagiri S, Eto K, et al. Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. Neuropathology. 2014;34:140-7 pubmed
  23. Kitamura A, Inada N, Kubota H, Matsumoto G, Kinjo M, Morimoto R, et al. Dysregulation of the proteasome increases the toxicity of ALS-linked mutant SOD1. Genes Cells. 2014;19:209-24 pubmed publisher
  24. Lai A, Lan C, Hasan S, Brown M, McLaurin J. scyllo-Inositol promotes robust mutant Huntingtin protein degradation. J Biol Chem. 2014;289:3666-76 pubmed publisher
  25. Li L, Yang G, Ren C, Tanimoto R, Hirayama T, Wang J, et al. Glioma pathogenesis-related protein 1 induces prostate cancer cell death through Hsc70-mediated suppression of AURKA and TPX2. Mol Oncol. 2013;7:484-96 pubmed publisher
  26. Reina C, Nabet B, Young P, Pittman R. Basal and stress-induced Hsp70 are modulated by ataxin-3. Cell Stress Chaperones. 2012;17:729-42 pubmed publisher