This is a Validated Antibody Database (VAD) review about dog EPS8, based on 16 published articles (read how Labome selects the articles), using EPS8 antibody in all methods. It is aimed to help Labome visitors find the most suited EPS8 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EPS8 synonym: epidermal growth factor receptor kinase substrate 8; LOW QUALITY PROTEIN: epidermal growth factor receptor kinase substrate 8

Knockout validation
BD Biosciences
mouse monoclonal (15/Eps8)
  • immunocytochemistry knockout validation; mouse; fig 1
  • western blot knockout validation; mouse; fig 2
  • immunoprecipitation; mouse; fig 6
BD Biosciences EPS8 antibody (BD, 610144) was used in immunocytochemistry knockout validation on mouse samples (fig 1), in western blot knockout validation on mouse samples (fig 2) and in immunoprecipitation on mouse samples (fig 6). J Cell Biol (2015) ncbi
BD Biosciences
mouse monoclonal (15/Eps8)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 1i
BD Biosciences EPS8 antibody (BD Transduction, 610143) was used in immunohistochemistry on mouse samples at 1:200 (fig 1i). Nat Commun (2017) ncbi
mouse monoclonal (15/Eps8)
  • immunocytochemistry; rat; 1:50; loading ...; fig 4a
  • western blot; rat; 1:5000; loading ...; fig 3a
In order to explore how laminin2 in the basement membrane modulates the blood testis barrier dynamics during spermatogenesis, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunocytochemistry on rat samples at 1:50 (fig 4a) and in western blot on rat samples at 1:5000 (fig 3a). FASEB J (2017) ncbi
mouse monoclonal (15/Eps8)
  • immunocytochemistry; rat; 1:100; loading ...; tbl 1
  • western blot; rat; 1:500; loading ...; tbl 1
In order to examine the organization of actin microfilaments in Sertoli cell at the blood testis barrier, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunocytochemistry on rat samples at 1:100 (tbl 1) and in western blot on rat samples at 1:500 (tbl 1). Spermatogenesis (2016) ncbi
mouse monoclonal (15/Eps8)
  • immunocytochemistry; rat; 1:100; fig 5
  • western blot; rat; 1:3000; fig 1
In order to characterize overexpression of gap junction protein connexin 43 by rescue of perfluorooctanesulfonate (PFOS)-mediated sertoli cell injury, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunocytochemistry on rat samples at 1:100 (fig 5) and in western blot on rat samples at 1:3000 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (15/Eps8)
  • immunohistochemistry - frozen section; rat; 1:50; fig 2
  • immunoprecipitation; rat; fig 4
  • immunocytochemistry; rat; 1:50; fig 6
  • western blot; rat; 1:5000; fig 5
In order to report that crumbs homolog 3 is an actin microfilament regulator, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunohistochemistry - frozen section on rat samples at 1:50 (fig 2), in immunoprecipitation on rat samples (fig 4), in immunocytochemistry on rat samples at 1:50 (fig 6) and in western blot on rat samples at 1:5000 (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (15/Eps8)
  • immunoprecipitation; rat; 1:30; fig 2
  • immunocytochemistry; rat; 1:50; fig 8
  • immunohistochemistry; rat; 1:100; fig 2
  • western blot; rat; 1:5000; fig 2
In order to determine regulation of ectoplasmic specialization dynamics via its effects on actin microfilaments in the testes of male rats by planar cell polarity (PCP) protein Vangl2, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunoprecipitation on rat samples at 1:30 (fig 2), in immunocytochemistry on rat samples at 1:50 (fig 8), in immunohistochemistry on rat samples at 1:100 (fig 2) and in western blot on rat samples at 1:5000 (fig 2). Endocrinology (2016) ncbi
mouse monoclonal (15/Eps8)
  • immunohistochemistry - frozen section; rat; 1:100; fig 3
In order to study transport of spermatids and residual bodies/phagosomes during spermatogenesis by coordination of microtuble-and actin-based cytoskeletons in rat testis, BD Biosciences EPS8 antibody (BD Biosciences, 610 143) was used in immunohistochemistry - frozen section on rat samples at 1:100 (fig 3). Endocrinology (2016) ncbi
mouse monoclonal (15/Eps8)
  • immunocytochemistry; rat; 1:100; fig 5
In order to characterize disruption of the sertolic cell cytoskeleton by interleukin 1alpha and its affect on gap junctional communication, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunocytochemistry on rat samples at 1:100 (fig 5). Cell Signal (2016) ncbi
mouse monoclonal (15/Eps8)
  • other; rat; 1:50; tbl 2
  • immunohistochemistry - frozen section; rat; 1:50; tbl 2
In order to test if overexpression of Connexin 43 in adjudin-treated rats reseals the disrupted blood-testis barrier and reinitiates spermatogenesis, BD Biosciences EPS8 antibody (BD Biosciences, 610144) was used in other on rat samples at 1:50 (tbl 2) and in immunohistochemistry - frozen section on rat samples at 1:50 (tbl 2). FASEB J (2016) ncbi
mouse monoclonal (15/Eps8)
  • immunocytochemistry knockout validation; mouse; fig 1
  • western blot knockout validation; mouse; fig 2
  • immunoprecipitation; mouse; fig 6
BD Biosciences EPS8 antibody (BD, 610144) was used in immunocytochemistry knockout validation on mouse samples (fig 1), in western blot knockout validation on mouse samples (fig 2) and in immunoprecipitation on mouse samples (fig 6). J Cell Biol (2015) ncbi
mouse monoclonal (15/Eps8)
  • western blot; rat; fig 3
BD Biosciences EPS8 antibody (BD, 610144) was used in western blot on rat samples (fig 3). Front Cell Neurosci (2015) ncbi
mouse monoclonal (15/Eps8)
  • immunohistochemistry; mouse; loading ...; fig 4f
BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunohistochemistry on mouse samples (fig 4f). elife (2015) ncbi
mouse monoclonal (15/Eps8)
  • immunocytochemistry; human; fig 1
BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunocytochemistry on human samples (fig 1). elife (2015) ncbi
mouse monoclonal (15/Eps8)
  • immunohistochemistry; rat; 1:50
  • western blot; rat; 1:5000
In order to study the role of plastin 3 in ectoplasmic specialization dynamics during spermatogenesis in the rat testis, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunohistochemistry on rat samples at 1:50 and in western blot on rat samples at 1:5000. FASEB J (2015) ncbi
mouse monoclonal (15/Eps8)
  • immunohistochemistry - frozen section; rat; 1:100
  • western blot; rat; 1:5000
In order to study the role of EB1 in tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier, BD Biosciences EPS8 antibody (BD Biosciences, 610143) was used in immunohistochemistry - frozen section on rat samples at 1:100 and in western blot on rat samples at 1:5000. Endocrinology (2015) ncbi
mouse monoclonal (15/Eps8)
  • western blot; rat; 1:1000
In order to investigate their role of angiopoietin 1 in regulating the blood-spinal cord barrier, BD Biosciences EPS8 antibody (BD, 610,143) was used in western blot on rat samples at 1:1000. Exp Cell Res (2014) ncbi
Articles Reviewed
  1. Mauriac S, Hien Y, Bird J, Carvalho S, Peyroutou R, Lee S, et al. Defective Gpsm2/G?i3 signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome. Nat Commun. 2017;8:14907 pubmed publisher
  2. Gao Y, Mruk D, Chen H, Lui W, Lee W, Cheng C. Regulation of the blood-testis barrier by a local axis in the testis: role of laminin ?2 in the basement membrane. FASEB J. 2017;31:584-597 pubmed publisher
  3. Li N, Lee W, Cheng C. Overexpression of plastin 3 in Sertoli cells disrupts actin microfilament bundle homeostasis and perturbs the tight junction barrier. Spermatogenesis. 2016;6:e1206353 pubmed publisher
  4. Li N, Mruk D, Chen H, Wong C, Lee W, Cheng C. Rescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43. Sci Rep. 2016;6:29667 pubmed publisher
  5. Gao Y, Lui W, Lee W, Cheng C. Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells. Sci Rep. 2016;6:28589 pubmed publisher
  6. Chen H, Mruk D, Lee W, Cheng C. Planar Cell Polarity (PCP) Protein Vangl2 Regulates Ectoplasmic Specialization Dynamics via Its Effects on Actin Microfilaments in the Testes of Male Rats. Endocrinology. 2016;157:2140-59 pubmed publisher
  7. Tang E, Lee W, Cheng C. Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis. Endocrinology. 2016;157:1644-59 pubmed publisher
  8. Chojnacka K, Bilinska B, Mruk D. Interleukin 1alpha-induced disruption of the Sertoli cell cytoskeleton affects gap junctional communication. Cell Signal. 2016;28:469-480 pubmed publisher
  9. Li N, Mruk D, Mok K, Li M, Wong C, Lee W, et al. Connexin 43 reboots meiosis and reseals blood-testis barrier following toxicant-mediated aspermatogenesis and barrier disruption. FASEB J. 2016;30:1436-52 pubmed publisher
  10. Giampietro C, Disanza A, Bravi L, Barrios Rodiles M, Corada M, Frittoli E, et al. The actin-binding protein EPS8 binds VE-cadherin and modulates YAP localization and signaling. J Cell Biol. 2015;211:1177-92 pubmed publisher
  11. Garcia Manteiga J, Bonfiglio S, Folladori L, Malosio M, Lazarevic D, Stupka E, et al. REST-Governed Gene Expression Profiling in a Neuronal Cell Model Reveals Novel Direct and Indirect Processes of Repression and Up-Regulation. Front Cell Neurosci. 2015;9:438 pubmed publisher
  12. Fang Q, Indzhykulian A, Mustapha M, Riordan G, Dolan D, Friedman T, et al. The 133-kDa N-terminal domain enables myosin 15 to maintain mechanotransducing stereocilia and is essential for hearing. elife. 2015;4: pubmed publisher
  13. Logue J, Cartagena Rivera A, Baird M, Davidson M, Chadwick R, Waterman C. Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration. elife. 2015;4:e08314 pubmed publisher
  14. Li N, Mruk D, Wong C, Lee W, Han D, Cheng C. Actin-bundling protein plastin 3 is a regulator of ectoplasmic specialization dynamics during spermatogenesis in the rat testis. FASEB J. 2015;29:3788-805 pubmed publisher
  15. Tang E, Mok K, Lee W, Cheng C. EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study. Endocrinology. 2015;156:680-93 pubmed publisher
  16. Liu X, Zhou X, Yuan W. The angiopoietin1-Akt pathway regulates barrier function of the cultured spinal cord microvascular endothelial cells through Eps8. Exp Cell Res. 2014;328:118-31 pubmed publisher