This is a Validated Antibody Database (VAD) review about dog DAG1, based on 29 published articles (read how Labome selects the articles), using DAG1 antibody in all methods. It is aimed to help Labome visitors find the most suited DAG1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DAG1 synonym: dystroglycan; dystrophin-associated glycoprotein 1; glycine cleavage system T-protein

Santa Cruz Biotechnology
mouse monoclonal (IIH6)
  • immunohistochemistry; rabbit; loading ...; fig 2??
Santa Cruz Biotechnology DAG1 antibody (Santa Cruz, sc-53987) was used in immunohistochemistry on rabbit samples (fig 2??). Dis Model Mech (2018) ncbi
mouse monoclonal (IIH6)
  • western blot; mouse; 1:500; fig 5
Santa Cruz Biotechnology DAG1 antibody (Santa Cruz, sc-53987) was used in western blot on mouse samples at 1:500 (fig 5). J Am Heart Assoc (2015) ncbi
mouse monoclonal (IIH6)
  • western blot; mouse; 1:500; fig 4
Santa Cruz Biotechnology DAG1 antibody (Santa Cruz, sc-53987) was used in western blot on mouse samples at 1:500 (fig 4). Hum Mol Genet (2015) ncbi
EMD Millipore
mouse monoclonal (IIH6C4)
  • immunohistochemistry - frozen section; mouse; 1:100; fig s3c
EMD Millipore DAG1 antibody (Millipore, 05-593) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig s3c). J Clin Invest (2017) ncbi
mouse monoclonal (IIH6C4)
  • western blot; mouse; loading ...; fig 3
In order to propose that postnatal gene therapy improves spatial learning even in the presence of neuronal ectopia, EMD Millipore DAG1 antibody (Millipore, 05-593) was used in western blot on mouse samples (fig 3). Genes (Basel) (2016) ncbi
mouse monoclonal (IIH6C4)
  • immunohistochemistry - paraffin section; mouse; 1:500; loading ...; fig 3 a","b
  • western blot; mouse; 1:2000; loading ...; fig s1 B",:A
In order to study diaphragm and cardiac function in mice with a mutated fukutin-related protein gene, EMD Millipore DAG1 antibody (Millipore, 05-593) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 3 a","b) and in western blot on mouse samples at 1:2000 (fig s1 B",:A). PLoS ONE (2016) ncbi
mouse monoclonal (IIH6C4)
  • western blot; mouse; 1:500; fig 6
In order to report the mechanism by which thrombospondin-4 regulates skeletal muscle integrity, EMD Millipore DAG1 antibody (Millipore, 05-593) was used in western blot on mouse samples at 1:500 (fig 6). elife (2016) ncbi
mouse monoclonal (IIH6C4)
  • flow cytometry; human; 1:100; fig s4
EMD Millipore DAG1 antibody (Merck Millipore, 05-593) was used in flow cytometry on human samples at 1:100 (fig s4). Nat Commun (2016) ncbi
mouse monoclonal (IIH6C4)
  • immunohistochemistry - frozen section; human; loading ...; fig 7fs2h
EMD Millipore DAG1 antibody (Millipore, IIH6) was used in immunohistochemistry - frozen section on human samples (fig 7fs2h). elife (2016) ncbi
mouse monoclonal (IIH6C4)
  • blocking or activating experiments; human; 10 ug/ml; loading ...; fig 4e
  • blocking or activating experiments; mouse; 10 ug/ml; fig 4a
In order to discover that agrin is a regulator of Eph receptor signaling and elucidate its mechanism of controlling cell-cell adhesion and red cell development, EMD Millipore DAG1 antibody (Millipore, IIH6C4) was used in blocking or activating experiments on human samples at 10 ug/ml (fig 4e) and in blocking or activating experiments on mouse samples at 10 ug/ml (fig 4a). Cell Death Differ (2016) ncbi
mouse monoclonal (IIH6C4)
  • immunohistochemistry; mouse; 1:50; fig 5
  • western blot; mouse; 1:500; fig 5, 6
In order to analyze how Serpina3n overexpression halts muscular dystrophy in mice, EMD Millipore DAG1 antibody (Millipore, 05-593) was used in immunohistochemistry on mouse samples at 1:50 (fig 5) and in western blot on mouse samples at 1:500 (fig 5, 6). Hum Mol Genet (2016) ncbi
mouse monoclonal (IIH6C4)
  • immunocytochemistry; rat; 1:100; fig 2
  • western blot; rat; 1:1500; fig s2
EMD Millipore DAG1 antibody (Millipore, 05-593) was used in immunocytochemistry on rat samples at 1:100 (fig 2) and in western blot on rat samples at 1:1500 (fig s2). Front Cell Neurosci (2015) ncbi
mouse monoclonal (IIH6C4)
  • other; rat; loading ...; fig 4a
  • immunocytochemistry; rat; loading ...; fig 1e
  • western blot; rat; loading ...; fig 1j
EMD Millipore DAG1 antibody (EMD Millipore, 05-593) was used in other on rat samples (fig 4a), in immunocytochemistry on rat samples (fig 1e) and in western blot on rat samples (fig 1j). J Cell Biol (2015) ncbi
mouse monoclonal (IIH6C4)
  • western blot; rat
EMD Millipore DAG1 antibody (Millipore, 05-593) was used in western blot on rat samples . Physiol Rep (2014) ncbi
mouse monoclonal (IIH6C4)
  • immunohistochemistry - frozen section; mouse
EMD Millipore DAG1 antibody (EMD Millipore, IIH6C4) was used in immunohistochemistry - frozen section on mouse samples . Hum Gene Ther Methods (2014) ncbi
mouse monoclonal (IIH6C4)
  • immunohistochemistry - frozen section; human
EMD Millipore DAG1 antibody (Millipore, IIH6C4) was used in immunohistochemistry - frozen section on human samples . J Med Genet (2014) ncbi
mouse monoclonal (IIH6C4)
  • immunohistochemistry - frozen section; mouse; 1:100
  • western blot; mouse; 1:1000
EMD Millipore DAG1 antibody (Millipore, IIH6C4) was used in immunohistochemistry - frozen section on mouse samples at 1:100 and in western blot on mouse samples at 1:1000. J Muscle Res Cell Motil (2013) ncbi
mouse monoclonal (IIH6C4)
  • western blot; mouse; 1:5000
EMD Millipore DAG1 antibody (Millipore, IIH6C4) was used in western blot on mouse samples at 1:5000. Brain Res (2013) ncbi
mouse monoclonal (IIH6C4)
  • western blot; human
EMD Millipore DAG1 antibody (Merck Millipore, 05-593) was used in western blot on human samples . Brain (2013) ncbi
Leica Biosystems
mouse monoclonal (43DAG/8D5)
  • immunohistochemistry; dog; 1:20; loading ...; fig s15
Leica Biosystems DAG1 antibody (Novocastra, 43DAG/8D5) was used in immunohistochemistry on dog samples at 1:20 (fig s15). Science (2018) ncbi
mouse monoclonal (43DAG/8D5)
  • western blot; mouse; loading ...; fig 1a
Leica Biosystems DAG1 antibody (Leica Biosystems, 43DAG1/8D5) was used in western blot on mouse samples (fig 1a). Biochem Biophys Res Commun (2017) ncbi
mouse monoclonal (43DAG/8D5)
  • western blot; rat; 1:500; fig 5
Leica Biosystems DAG1 antibody (Novocastra, NCL-b-DG) was used in western blot on rat samples at 1:500 (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (43DAG/8D5)
  • immunohistochemistry - frozen section; human; 1:50; fig 4
Leica Biosystems DAG1 antibody (Novocastra, NCL-b-DG) was used in immunohistochemistry - frozen section on human samples at 1:50 (fig 4). PLoS ONE (2016) ncbi
mouse monoclonal (43DAG/8D5)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig s7
In order to study in vivo gene editing in dystrophic mouse muscle and muscle stem cells, Leica Biosystems DAG1 antibody (Novocatra, NCL-b-DG) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig s7). Science (2016) ncbi
mouse monoclonal (43DAG/8D5)
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig s8
In order to present the role of in vivo genome editing in muscle function in a mouse model of Duchenne muscular dystrophy, Leica Biosystems DAG1 antibody (Novocastra, 43DAG1/8D5) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig s8). Science (2016) ncbi
mouse monoclonal (43DAG/8D5)
  • immunohistochemistry; human; 1:500; loading ...; tbl 1
Leica Biosystems DAG1 antibody (Novocastra Laboratories, NCL-b-DG) was used in immunohistochemistry on human samples at 1:500 (tbl 1). J Anat (2016) ncbi
mouse monoclonal (43DAG/8D5)
  • western blot; rat; 1:40; fig s2d
In order to identify novel molecules in peripheral myelination by spatial mapping of juxtacrine axo-glial interactions, Leica Biosystems DAG1 antibody (Novocastra, Ncl-b-DG) was used in western blot on rat samples at 1:40 (fig s2d). Nat Commun (2015) ncbi
mouse monoclonal (43DAG/8D5)
  • western blot; mouse
Leica Biosystems DAG1 antibody (Novocastra, NCL-b-DG) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
mouse monoclonal (43DAG/8D5)
  • immunohistochemistry - frozen section; Rhesus monkey; 1:100
Leica Biosystems DAG1 antibody (Novocastra, NCL-b-DG 8) was used in immunohistochemistry - frozen section on Rhesus monkey samples at 1:100. J Comp Neurol (2011) ncbi
Articles Reviewed
  1. Amoasii L, Hildyard J, Li H, Sanchez Ortiz E, Mireault A, Caballero D, et al. Gene editing restores dystrophin expression in a canine model of Duchenne muscular dystrophy. Science. 2018;362:86-91 pubmed publisher
  2. Sui T, Lau Y, Liu D, Liu T, Xu L, Gao Y, et al. A novel rabbit model of Duchenne muscular dystrophy generated by CRISPR/Cas9. Dis Model Mech. 2018;11: pubmed publisher
  3. Fukai Y, Ohsawa Y, Ohtsubo H, Nishimatsu S, Hagiwara H, Noda M, et al. Cleavage of ?-dystroglycan occurs in sarcoglycan-deficient skeletal muscle without MMP-2 and MMP-9. Biochem Biophys Res Commun. 2017;492:199-205 pubmed publisher
  4. McKee K, Crosson S, Meinen S, Reinhard J, Ruegg M, Yurchenco P. Chimeric protein repair of laminin polymerization ameliorates muscular dystrophy phenotype. J Clin Invest. 2017;127:1075-1089 pubmed publisher
  5. Hu H, Liu Y, Bampoe K, He Y, Yu M. Postnatal Gene Therapy Improves Spatial Learning Despite the Presence of Neuronal Ectopia in a Model of Neuronal Migration Disorder. Genes (Basel). 2016;7: pubmed
  6. Blaeser A, Awano H, Wu B, Lu Q. Progressive Dystrophic Pathology in Diaphragm and Impairment of Cardiac Function in FKRP P448L Mutant Mice. PLoS ONE. 2016;11:e0164187 pubmed publisher
  7. Vanhoutte D, Schips T, Kwong J, Davis J, Tjondrokoesoemo A, Brody M, et al. Thrombospondin expression in myofibers stabilizes muscle membranes. elife. 2016;5: pubmed publisher
  8. Magnowska M, Gorkiewicz T, Suska A, Wawrzyniak M, Rutkowska Wlodarczyk I, Kaczmarek L, et al. Transient ECM protease activity promotes synaptic plasticity. Sci Rep. 2016;6:27757 pubmed publisher
  9. Gerin I, Ury B, Breloy I, Bouchet Seraphin C, Bolsée J, Halbout M, et al. ISPD produces CDP-ribitol used by FKTN and FKRP to transfer ribitol phosphate onto ?-dystroglycan. Nat Commun. 2016;7:11534 pubmed publisher
  10. Praissman J, Willer T, Sheikh M, Toi A, Chitayat D, Lin Y, et al. The functional O-mannose glycan on ?-dystroglycan contains a phospho-ribitol primed for matriglycan addition. elife. 2016;5: pubmed publisher
  11. Anselmo A, Lauranzano E, Soldani C, Ploia C, Angioni R, D Amico G, et al. Identification of a novel agrin-dependent pathway in cell signaling and adhesion within the erythroid niche. Cell Death Differ. 2016;23:1322-30 pubmed publisher
  12. Janghra N, Morgan J, Sewry C, Wilson F, Davies K, Muntoni F, et al. Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies. PLoS ONE. 2016;11:e0150818 pubmed publisher
  13. Tjondrokoesoemo A, Schips T, Kanisicak O, Sargent M, Molkentin J. Genetic overexpression of Serpina3n attenuates muscular dystrophy in mice. Hum Mol Genet. 2016;25:1192-202 pubmed publisher
  14. Tabebordbar M, Zhu K, Cheng J, Chew W, Widrick J, Yan W, et al. In vivo gene editing in dystrophic mouse muscle and muscle stem cells. Science. 2016;351:407-411 pubmed publisher
  15. Nelson C, Hakim C, Ousterout D, Thakore P, Moreb E, Castellanos Rivera R, et al. In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy. Science. 2016;351:403-7 pubmed publisher
  16. Parvatiyar M, Marshall J, Nguyen R, Jordan M, Richardson V, Roos K, et al. Sarcospan Regulates Cardiac Isoproterenol Response and Prevents Duchenne Muscular Dystrophy-Associated Cardiomyopathy. J Am Heart Assoc. 2015;4: pubmed publisher
  17. Shah F, Berggren D, Holmlund T, Levring Jäghagen E, Stål P. Unique expression of cytoskeletal proteins in human soft palate muscles. J Anat. 2016;228:487-94 pubmed publisher
  18. Poitelon Y, Bogni S, Matafora V, Della Flora Nunes G, Hurley E, Ghidinelli M, et al. Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination. Nat Commun. 2015;6:8303 pubmed publisher
  19. Bijata M, Wlodarczyk J, Figiel I. Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro. Front Cell Neurosci. 2015;9:199 pubmed publisher
  20. Colombelli C, Palmisano M, Eshed Eisenbach Y, Zambroni D, Pavoni E, Ferri C, et al. Perlecan is recruited by dystroglycan to nodes of Ranvier and binds the clustering molecule gliomedin. J Cell Biol. 2015;208:313-29 pubmed publisher
  21. Marshall J, Oh J, Chou E, Lee J, Holmberg J, Burkin D, et al. Sarcospan integration into laminin-binding adhesion complexes that ameliorate muscular dystrophy requires utrophin and α7 integrin. Hum Mol Genet. 2015;24:2011-22 pubmed publisher
  22. Ogasawara R, Nakazato K, Sato K, Boppart M, Fujita S. Resistance exercise increases active MMP and β1-integrin protein expression in skeletal muscle. Physiol Rep. 2014;2: pubmed publisher
  23. Sharma P, Basu S, Mitchell R, Stelmack G, Anderson J, Halayko A. Role of dystrophin in airway smooth muscle phenotype, contraction and lung function. PLoS ONE. 2014;9:e102737 pubmed publisher
  24. Vannoy C, Xu L, Keramaris E, Lu P, Xiao X, Lu Q. Adeno-associated virus-mediated overexpression of LARGE rescues ?-dystroglycan function in dystrophic mice with mutations in the fukutin-related protein. Hum Gene Ther Methods. 2014;25:187-96 pubmed publisher
  25. von Renesse A, Petkova M, Lützkendorf S, Heinemeyer J, Gill E, Hübner C, et al. POMK mutation in a family with congenital muscular dystrophy with merosin deficiency, hypomyelination, mild hearing deficit and intellectual disability. J Med Genet. 2014;51:275-82 pubmed publisher
  26. Sharpe K, Premsukh M, Townsend D. Alterations of dystrophin-associated glycoproteins in the heart lacking dystrophin or dystrophin and utrophin. J Muscle Res Cell Motil. 2013;34:395-405 pubmed publisher
  27. Hawkins B, Gu Y, Izawa Y, del Zoppo G. Disruption of dystroglycan-laminin interactions modulates water uptake by astrocytes. Brain Res. 2013;1503:89-96 pubmed publisher
  28. Cirak S, Foley A, Herrmann R, Willer T, Yau S, Stevens E, et al. ISPD gene mutations are a common cause of congenital and limb-girdle muscular dystrophies. Brain. 2013;136:269-81 pubmed publisher
  29. Puthussery T, Gayet Primo J, Taylor W, Haverkamp S. Immunohistochemical identification and synaptic inputs to the diffuse bipolar cell type DB1 in macaque retina. J Comp Neurol. 2011;519:3640-56 pubmed publisher