This is a Validated Antibody Database (VAD) review about dog ANXA2, based on 18 published articles (read how Labome selects the articles), using ANXA2 antibody in all methods. It is aimed to help Labome visitors find the most suited ANXA2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ANXA2 synonym: annexin A2; annexin 2

Knockout validation
BD Biosciences
mouse monoclonal (5/Annexin II)
  • immunocytochemistry knockout validation; mouse; fig 1
  • immunohistochemistry knockout validation; mouse; fig 1
BD Biosciences ANXA2 antibody (BD Pharmingen), 610069) was used in immunocytochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry knockout validation on mouse samples (fig 1). Mol Cancer Res (2015) ncbi
Invitrogen
mouse monoclonal (Z014)
  • immunohistochemistry - frozen section; mouse; loading ...
  • flow cytometry; mouse; 1:50; loading ...; fig 6a
In order to identify annexin A2 as a binding partner of factor H, Invitrogen ANXA2 antibody (Invitrogen, Z014) was used in immunohistochemistry - frozen section on mouse samples and in flow cytometry on mouse samples at 1:50 (fig 6a). J Immunol (2016) ncbi
mouse monoclonal (Z014)
  • blocking or activating experiments; human; 25 ug/ml
In order to examine the effect of interferon-gamma treatment on cell surface protein expression using prostate cancer cells, Invitrogen ANXA2 antibody (Zymed Laboratories, 034400) was used in blocking or activating experiments on human samples at 25 ug/ml. J Biol Chem (2008) ncbi
mouse monoclonal (Z014)
  • blocking or activating experiments; human; fig 1
In order to show that the alpha(M)beta(2) regulates neutrophil-dependent fibrinolysis, Invitrogen ANXA2 antibody (Zymed, Z014) was used in blocking or activating experiments on human samples (fig 1). J Biol Chem (2004) ncbi
mouse monoclonal (Z014)
  • western blot; mouse; 0.125 ug/ml; fig 6
  • western blot; human; 0.125 ug/ml
In order to assess mediation of plasminogen-dependent matrix invasion by human monocytes by annexin II as enhanced expression by macrophages, Invitrogen ANXA2 antibody (Zymed, noca) was used in western blot on mouse samples at 0.125 ug/ml (fig 6) and in western blot on human samples at 0.125 ug/ml. Blood (2004) ncbi
mouse monoclonal (Z014)
  • western blot; cow
In order to examine the effect of nitric oxide donors on the activities of annexin II tetramer, Invitrogen ANXA2 antibody (Zymed, 034400) was used in western blot on cow samples . Eur J Biochem (2002) ncbi
mouse monoclonal (Z014)
  • western blot; human; fig 1
In order to test if annexin II is released from syncytiotrophoblast microvillous plasma membranes and if it has any immunosuppressive effect, Invitrogen ANXA2 antibody (Zymed Laboratories, 03-4400) was used in western blot on human samples (fig 1). Am J Reprod Immunol (1997) ncbi
Cell Signaling Technology
rabbit monoclonal (D11G2)
  • immunoprecipitation; mouse; loading ...; fig s5a
  • western blot; mouse; loading ...; fig s5a
Cell Signaling Technology ANXA2 antibody (Cell Signaling, 8235) was used in immunoprecipitation on mouse samples (fig s5a) and in western blot on mouse samples (fig s5a). Sci Rep (2018) ncbi
rabbit monoclonal (D11G2)
  • immunocytochemistry; human
In order to study retinal degeneration caused by disruption of the microtubule-binding and membrane-binding domains of CEP290, Cell Signaling Technology ANXA2 antibody (Cell Signaling, 8235) was used in immunocytochemistry on human samples . J Clin Invest (2013) ncbi
BD Biosciences
mouse monoclonal (5/Annexin II)
  • western blot; mouse; fig 4a
In order to describe extracellular vesicle subpopulations secreted by fat cells, BD Biosciences ANXA2 antibody (BD Biosciences, 610068) was used in western blot on mouse samples (fig 4a). J Extracell Vesicles (2017) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; rat; 1:1000; loading ...; fig 4b
In order to investigate the role of Ser25 phosphorylation in subcellular localization of Annexin A2 and its interaction with mRNP complexes, BD Biosciences ANXA2 antibody (BD Biosciences, 610069) was used in western blot on rat samples at 1:1000 (fig 4b). FEBS Open Bio (2017) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; human; loading ...; fig 1a
BD Biosciences ANXA2 antibody (BD Transduction Laboratories, 610068) was used in western blot on human samples (fig 1a). PLoS ONE (2016) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; human; loading ...; fig 4
BD Biosciences ANXA2 antibody (BD Transduction lab, 610069) was used in western blot on human samples (fig 4). Oncotarget (2016) ncbi
mouse monoclonal (5/Annexin II)
  • western blot; rat; 1:1000; fig 6
In order to study how Tyr23 phosphorylation regulates AnxA2 function, BD Biosciences ANXA2 antibody (BD Biosciences, 610069) was used in western blot on rat samples at 1:1000 (fig 6). J Cell Sci (2016) ncbi
mouse monoclonal (5/Annexin II)
  • immunocytochemistry; human; fig 3
  • immunohistochemistry; human; fig 5
  • western blot; human; fig 4
In order to demonstrate the transcriptional repression of ANXA2 by ERG in prostate epithelial cells and discuss how this interaction relates to prostate cancer, BD Biosciences ANXA2 antibody (BD Biosciences, 610068) was used in immunocytochemistry on human samples (fig 3), in immunohistochemistry on human samples (fig 5) and in western blot on human samples (fig 4). Mol Cancer Res (2015) ncbi
mouse monoclonal (5/Annexin II)
  • immunocytochemistry knockout validation; mouse; fig 1
  • immunohistochemistry knockout validation; mouse; fig 1
BD Biosciences ANXA2 antibody (BD Pharmingen), 610069) was used in immunocytochemistry knockout validation on mouse samples (fig 1) and in immunohistochemistry knockout validation on mouse samples (fig 1). Mol Cancer Res (2015) ncbi
mouse monoclonal (5/Annexin II)
  • immunohistochemistry - paraffin section; human; 1:1,000
  • western blot; human; 1:5,000
BD Biosciences ANXA2 antibody (BD Biosciences, 610069) was used in immunohistochemistry - paraffin section on human samples at 1:1,000 and in western blot on human samples at 1:5,000. Biomed Res (2014) ncbi
mouse monoclonal (5/Annexin II)
  • immunocytochemistry; human
In order to demonstrate that Listeria monocytogenes uses efferocytosis to promote cell-to-cell spread during infection, BD Biosciences ANXA2 antibody (BD Biosciences, 610068) was used in immunocytochemistry on human samples . Nature (2014) ncbi
mouse monoclonal (6/p36/MAT1)
  • western blot; human; 1:4000
BD Biosciences ANXA2 antibody (BD Biosciences, 610532) was used in western blot on human samples at 1:4000. Clin Exp Immunol (2014) ncbi
Articles Reviewed
  1. Hakuno D, Kimura M, Ito S, Satoh J, Nakashima Y, Horie T, et al. Hepatokine α1-Microglobulin Signaling Exacerbates Inflammation and Disturbs Fibrotic Repair in Mouse Myocardial Infarction. Sci Rep. 2018;8:16749 pubmed publisher
  2. Durcin M, Fleury A, Taillebois E, Hilairet G, Krupova Z, Henry C, et al. Characterisation of adipocyte-derived extracellular vesicle subtypes identifies distinct protein and lipid signatures for large and small extracellular vesicles. J Extracell Vesicles. 2017;6:1305677 pubmed publisher
  3. Aukrust I, Rosenberg L, Ankerud M, Bertelsen V, Hollås H, Saraste J, et al. Post-translational modifications of Annexin A2 are linked to its association with perinuclear nonpolysomal mRNP complexes. FEBS Open Bio. 2017;7:160-173 pubmed publisher
  4. Dismuke W, Klingeborn M, Stamer W. Mechanism of Fibronectin Binding to Human Trabecular Meshwork Exosomes and Its Modulation by Dexamethasone. PLoS ONE. 2016;11:e0165326 pubmed publisher
  5. Madureira P, Bharadwaj A, Bydoun M, Garant K, O Connell P, Lee P, et al. Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10. Oncotarget. 2016;7:47720-47737 pubmed publisher
  6. Renner B, Tong H, Laskowski J, Jonscher K, Goetz L, Woolaver R, et al. Annexin A2 Enhances Complement Activation by Inhibiting Factor H. J Immunol. 2016;196:1355-65 pubmed publisher
  7. Grindheim A, Hollås H, Raddum A, Saraste J, Vedeler A. Reactive oxygen species exert opposite effects on Tyr23 phosphorylation of the nuclear and cortical pools of annexin A2. J Cell Sci. 2016;129:314-28 pubmed publisher
  8. Griner N, Young D, Chaudhary P, Mohamed A, Huang W, Chen Y, et al. ERG oncoprotein inhibits ANXA2 expression and function in prostate cancer. Mol Cancer Res. 2015;13:368-79 pubmed publisher
  9. Jung Y, Wang J, Lee E, McGee S, Berry J, Yumoto K, et al. Annexin 2-CXCL12 interactions regulate metastatic cell targeting and growth in the bone marrow. Mol Cancer Res. 2015;13:197-207 pubmed publisher
  10. Kobayashi M, Nagashio R, Ryuge S, Murakami Y, Yanagita K, Nakashima H, et al. Acquisition of useful sero-diagnostic autoantibodies using the same patients'sera and tumor tissues. Biomed Res. 2014;35:133-43 pubmed
  11. Czuczman M, Fattouh R, van Rijn J, Canadien V, Osborne S, Muise A, et al. Listeria monocytogenes exploits efferocytosis to promote cell-to-cell spread. Nature. 2014;509:230-4 pubmed publisher
  12. Zouk H, d Hennezel E, Du X, Ounissi Benkalha H, Piccirillo C, Polychronakos C. Functional evaluation of the role of C-type lectin domain family 16A at the chromosome 16p13 locus. Clin Exp Immunol. 2014;175:485-97 pubmed publisher
  13. Drivas T, Holzbaur E, Bennett J. Disruption of CEP290 microtubule/membrane-binding domains causes retinal degeneration. J Clin Invest. 2013;123:4525-39 pubmed publisher
  14. Hastie C, Masters J, Moss S, Naaby Hansen S. Interferon-gamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem. 2008;283:12595-603 pubmed publisher
  15. Pluskota E, Soloviev D, Bdeir K, Cines D, Plow E. Integrin alphaMbeta2 orchestrates and accelerates plasminogen activation and fibrinolysis by neutrophils. J Biol Chem. 2004;279:18063-72 pubmed
  16. Brownstein C, Deora A, Jacovina A, Weintraub R, Gertler M, Khan K, et al. Annexin II mediates plasminogen-dependent matrix invasion by human monocytes: enhanced expression by macrophages. Blood. 2004;103:317-24 pubmed
  17. Liu L, Enright E, Sun P, Tsai S, Mehta P, Beckman D, et al. Inactivation of annexin II tetramer by S-nitrosoglutathione. Eur J Biochem. 2002;269:4277-86 pubmed
  18. Aarli A, Kristoffersen E, Jensen T, Ulvestad E, Matre R. Suppressive effect on lymphoproliferation in vitro by soluble annexin II released from isolated placental membranes. Am J Reprod Immunol. 1997;38:313-9 pubmed