This is a Validated Antibody Database (VAD) review about cow SMAD1, based on 14 published articles (read how Labome selects the articles), using SMAD1 antibody in all methods. It is aimed to help Labome visitors find the most suited SMAD1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SMAD1 synonym: mothers against decapentaplegic homolog 1; MAD homolog 1; SMAD 1; SMAD, mothers against DPP homolog 1; Sma- and Mad-related protein 1; mad-related protein 1; mothers against DPP homolog 1

Abcam
rabbit polyclonal
  • western blot; mouse; fig 1
Abcam SMAD1 antibody (Abcam, ab131371) was used in western blot on mouse samples (fig 1). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; rat; 1:400
Abcam SMAD1 antibody (Abcam, ab46688) was used in western blot on rat samples at 1:400. Mol Med Rep (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D59D7)
  • western blot; human; loading ...; fig 6d
Cell Signaling Technology SMAD1 antibody (Cell signaling, 6944) was used in western blot on human samples (fig 6d). Oncotarget (2017) ncbi
rabbit monoclonal (D59D7)
  • western blot; human; 1:20,000; loading ...; fig S1i
Cell Signaling Technology SMAD1 antibody (Cell signaling, 6944) was used in western blot on human samples at 1:20,000 (fig S1i). Nat Commun (2016) ncbi
rabbit monoclonal (D59D7)
  • western blot; human; 1:1000; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Cell Signaling Technology SMAD1 antibody (Cell Signaling, 6944) was used in western blot on human samples at 1:1000 (fig st1). Nat Commun (2016) ncbi
rabbit monoclonal (D59D7)
  • western blot; mouse; 1:1000; fig 3
Cell Signaling Technology SMAD1 antibody (Cell Signaling, 6944) was used in western blot on mouse samples at 1:1000 (fig 3). PLoS ONE (2016) ncbi
rabbit monoclonal (D59D7)
  • immunocytochemistry; mouse; loading ...; fig 1c
  • western blot; mouse; loading ...; fig 4c
In order to study transcriptional regulation of SMAD1 and 5 in endothelial cells, Cell Signaling Technology SMAD1 antibody (Cell Signaling, 6944) was used in immunocytochemistry on mouse samples (fig 1c) and in western blot on mouse samples (fig 4c). Mol Cell Biol (2015) ncbi
rabbit monoclonal (D59D7)
  • western blot; human
In order to report that L3MBTL1 represses the ability of stem cells to drive hematopoietic-specific transcriptional programs via SMAD5 and impairing its recruitment to target regulatory regions, Cell Signaling Technology SMAD1 antibody (Cell Signaling, 6944P) was used in western blot on human samples . Stem Cell Reports (2015) ncbi
rabbit monoclonal (D59D7)
  • western blot; mouse; 1:1000
In order to study heart valve development and cardiac function in Galnt1 KO mice, Cell Signaling Technology SMAD1 antibody (Cell Signaling, 6944) was used in western blot on mouse samples at 1:1000. PLoS ONE (2015) ncbi
rabbit monoclonal (D59D7)
  • western blot; human; loading ...; fig s3g
In order to investigate the role of Jmjd3 in T-cell differentiation, Cell Signaling Technology SMAD1 antibody (CST, 6944) was used in western blot on human samples (fig s3g). Nat Commun (2014) ncbi
rabbit monoclonal (D59D7)
  • chromatin immunoprecipitation; mouse; fig 4
In order to study the role of local BMP-SMAD1 signaling as it increases LIF receptor-dependent STAT3 responsiveness and pluripotent stem cell conversion frequencey in primed-to-naive mouse, Cell Signaling Technology SMAD1 antibody (Cell Signaling Technology, 6944) was used in chromatin immunoprecipitation on mouse samples (fig 4). Stem Cell Reports (2014) ncbi
rabbit monoclonal (D59D7)
  • western blot; human; 1:1000
Cell Signaling Technology SMAD1 antibody (Cell Signaling, 6944) was used in western blot on human samples at 1:1000. Nucleic Acids Res (2013) ncbi
rabbit monoclonal (D59D7)
  • western blot; human; 1:2000
Cell Signaling Technology SMAD1 antibody (Cell Signaling Technologies, 6944p) was used in western blot on human samples at 1:2000. PLoS ONE (2013) ncbi
rabbit monoclonal (D59D7)
  • western blot; common platanna
Cell Signaling Technology SMAD1 antibody (Cell Signaling, 6944) was used in western blot on common platanna samples . Development (2013) ncbi
Articles Reviewed
  1. Sun J, Liu X, Gao H, Zhang L, Ji Q, Wang Z, et al. Overexpression of colorectal cancer oncogene CHRDL2 predicts a poor prognosis. Oncotarget. 2017;8:11489-11506 pubmed publisher
  2. Mouillesseaux K, Wiley D, Saunders L, Wylie L, Kushner E, Chong D, et al. Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Nat Commun. 2016;7:13247 pubmed publisher
  3. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  4. Garrel G, Racine C, L hôte D, Denoyelle C, Guigon C, di Clemente N, et al. Anti-Müllerian hormone: a new actor of sexual dimorphism in pituitary gonadotrope activity before puberty. Sci Rep. 2016;6:23790 pubmed publisher
  5. West J, Carrier E, Bloodworth N, Schroer A, Chen P, Ryzhova L, et al. Serotonin 2B Receptor Antagonism Prevents Heritable Pulmonary Arterial Hypertension. PLoS ONE. 2016;11:e0148657 pubmed publisher
  6. Marks Bluth J, Khanna A, Chandrakanthan V, Thoms J, Bee T, Eich C, et al. SMAD1 and SMAD5 Expression Is Coordinately Regulated by FLI1 and GATA2 during Endothelial Development. Mol Cell Biol. 2015;35:2165-72 pubmed publisher
  7. Perna F, Vu L, Themeli M, Kriks S, Hoya Arias R, Khanin R, et al. The polycomb group protein L3MBTL1 represses a SMAD5-mediated hematopoietic transcriptional program in human pluripotent stem cells. Stem Cell Reports. 2015;4:658-69 pubmed publisher
  8. Tian E, Stevens S, Guan Y, Springer D, Anderson S, Starost M, et al. Galnt1 is required for normal heart valve development and cardiac function. PLoS ONE. 2015;10:e0115861 pubmed publisher
  9. Li Q, Zou J, Wang M, Ding X, Chepelev I, Zhou X, et al. Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T-cell differentiation. Nat Commun. 2014;5:5780 pubmed publisher
  10. Lu H, Lian L, Shi D, Zhao H, Dai Y. Hepcidin promotes osteogenic differentiation through the bone morphogenetic protein 2/small mothers against decapentaplegic and mitogen-activated protein kinase/P38 signaling pathways in mesenchymal stem cells. Mol Med Rep. 2015;11:143-50 pubmed publisher
  11. Onishi K, Tonge P, Nagy A, Zandstra P. Local BMP-SMAD1 signaling increases LIF receptor-dependent STAT3 responsiveness and primed-to-naive mouse pluripotent stem cell conversion frequency. Stem Cell Reports. 2014;3:156-68 pubmed publisher
  12. Hou P, Chuang C, Kao C, Chou S, Stone L, Ho H, et al. LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1. Nucleic Acids Res. 2013;41:7753-70 pubmed publisher
  13. Ren G, Beech C, Smas C. The immunoglobulin superfamily protein differentiation of embryonic stem cells 1 (dies1) has a regulatory role in preadipocyte to adipocyte conversion. PLoS ONE. 2013;8:e65531 pubmed publisher
  14. Lim C, Reversade B, Knowles B, Solter D. Optimal histone H3 to linker histone H1 chromatin ratio is vital for mesodermal competence in Xenopus. Development. 2013;140:853-60 pubmed publisher