This is a Validated Antibody Database (VAD) review about cow SIRT1, based on 12 published articles (read how Labome selects the articles), using SIRT1 antibody in all methods. It is aimed to help Labome visitors find the most suited SIRT1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SIRT1 synonym: NAD-dependent protein deacetylase sirtuin-1

Cell Signaling Technology
rabbit monoclonal (D1D7)
  • western blot; rat; 1:1000; loading ...; fig 4a
In order to measure brain insulin signaling in response to dexamethasone treatment in female Charles Foster rats, Cell Signaling Technology SIRT1 antibody (Cell Signaling, 9475) was used in western blot on rat samples at 1:1000 (fig 4a). Mol Neurobiol (2017) ncbi
rabbit monoclonal (D1D7)
  • immunoprecipitation; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 5i
Cell Signaling Technology SIRT1 antibody (Cell Signaling, D1D7) was used in immunoprecipitation on human samples (fig 2a) and in western blot on human samples (fig 5i). Oncogene (2017) ncbi
rabbit monoclonal (D1D7)
  • western blot; rat; 1:2000; loading ...; fig 5
Cell Signaling Technology SIRT1 antibody (Cell Signaling, 9475) was used in western blot on rat samples at 1:2000 (fig 5). Exp Ther Med (2016) ncbi
rabbit monoclonal (D1D7)
  • western blot; mouse; 1:1000; fig 2
Cell Signaling Technology SIRT1 antibody (Cell Signaling, 9475) was used in western blot on mouse samples at 1:1000 (fig 2). Sci Rep (2016) ncbi
rabbit monoclonal (D1D7)
  • western blot; mouse; fig 2a
In order to research the effects of environmental tobacco smoke on autophagy and longevity, Cell Signaling Technology SIRT1 antibody (Cell Signaling, 9475) was used in western blot on mouse samples (fig 2a). Oncotarget (2016) ncbi
rabbit monoclonal (D1D7)
  • western blot; mouse; 1:100; tbl 1
In order to elucidate a deletion of AMP-activated protein kinase in mouse Sertoli cells that modify germ cell quality, Cell Signaling Technology SIRT1 antibody (Cell Signalling, 9475) was used in western blot on mouse samples at 1:100 (tbl 1). Mol Cell Endocrinol (2016) ncbi
rabbit monoclonal (D1D7)
  • immunohistochemistry - paraffin section; mouse; 1:100
  • western blot; mouse; 1:1000
In order to describe a SUMO-specific protease-based methodology suitable for mass spectroscopy, Cell Signaling Technology SIRT1 antibody (Cell Signaling Technology, 9475P) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 and in western blot on mouse samples at 1:1000. Nat Commun (2015) ncbi
rabbit monoclonal (D1D7)
  • immunohistochemistry - paraffin section; mouse; 1:100
  • western blot; mouse; 1:1000
Cell Signaling Technology SIRT1 antibody (Cell Signaling Technology, 9475) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 and in western blot on mouse samples at 1:1000. Eur J Pharmacol (2015) ncbi
rabbit monoclonal (D1D7)
  • immunohistochemistry - paraffin section; rat; fig 7
  • immunocytochemistry; rat; fig 7
  • western blot; rat; 1:1000; fig 6
Cell Signaling Technology SIRT1 antibody (Cell Signaling, 9475) was used in immunohistochemistry - paraffin section on rat samples (fig 7), in immunocytochemistry on rat samples (fig 7) and in western blot on rat samples at 1:1000 (fig 6). PLoS ONE (2015) ncbi
rabbit monoclonal (D1D7)
  • western blot; human
Cell Signaling Technology SIRT1 antibody (Cell Signaling, 9475s) was used in western blot on human samples . J Diabetes (2016) ncbi
rabbit monoclonal (D1D7)
  • western blot; human
Cell Signaling Technology SIRT1 antibody (Cell Signaling, 9475) was used in western blot on human samples . Biomaterials (2015) ncbi
rabbit monoclonal (D1D7)
  • western blot; mouse; fig 1d
  • western blot; human; fig 1c
Cell Signaling Technology SIRT1 antibody (Cell signalling, 9475) was used in western blot on mouse samples (fig 1d) and in western blot on human samples (fig 1c). Am J Cancer Res (2014) ncbi
Articles Reviewed
  1. Chruvattil R, Banerjee S, Nath S, Machhi J, Kharkwal G, Yadav M, et al. Dexamethasone Alters the Appetite Regulation via Induction of Hypothalamic Insulin Resistance in Rat Brain. Mol Neurobiol. 2017;54:7483-7496 pubmed publisher
  2. Li Z, Bridges B, Olson J, Weinman S. The interaction between acetylation and serine-574 phosphorylation regulates the apoptotic function of FOXO3. Oncogene. 2017;36:1887-1898 pubmed publisher
  3. Liu M, Shan J, Li J, Zhang Y, Lin X. Resveratrol inhibits doxorubicin-induced cardiotoxicity via sirtuin 1 activation in H9c2 cardiomyocytes. Exp Ther Med. 2016;12:1113-1118 pubmed
  4. Wang K, Cao P, Wang H, Tang Z, Wang N, Wang J, et al. Chronic administration of Angelica sinensis polysaccharide effectively improves fatty liver and glucose homeostasis in high-fat diet-fed mice. Sci Rep. 2016;6:26229 pubmed publisher
  5. Ting W, Yang J, Kuo C, Xiao Z, Lu X, Yeh Y, et al. Environmental tobacco smoke increases autophagic effects but decreases longevity associated with Sirt-1 protein expression in young C57BL mice hearts. Oncotarget. 2016;7:39017-39025 pubmed publisher
  6. Bertoldo M, Guibert E, Faure M, Guillou F, Ramé C, Nadal Desbarats L, et al. Specific deletion of AMP-activated protein kinase (α1AMPK) in mouse Sertoli cells modifies germ cell quality. Mol Cell Endocrinol. 2016;423:96-112 pubmed publisher
  7. Hendriks I, D Souza R, Chang J, Mann M, Vertegaal A. System-wide identification of wild-type SUMO-2 conjugation sites. Nat Commun. 2015;6:7289 pubmed publisher
  8. Huang Z, Han Z, Ye B, Dai Z, Shan P, Lu Z, et al. Berberine alleviates cardiac ischemia/reperfusion injury by inhibiting excessive autophagy in cardiomyocytes. Eur J Pharmacol. 2015;762:1-10 pubmed publisher
  9. Zou D, Liu P, Chen K, Xie Q, Liang X, Bai Q, et al. Protective effects of myricetin on acute hypoxia-induced exercise intolerance and mitochondrial impairments in rats. PLoS ONE. 2015;10:e0124727 pubmed publisher
  10. Liu Z, Jiang C, Zhang J, Liu B, Du Q. Resveratrol inhibits inflammation and ameliorates insulin resistant endothelial dysfunction via regulation of AMP-activated protein kinase and sirtuin 1 activities. J Diabetes. 2016;8:324-35 pubmed publisher
  11. Mulens Arias V, Rojas J, Pérez Yagüe S, Morales M, Barber D. Polyethylenimine-coated SPIONs trigger macrophage activation through TLR-4 signaling and ROS production and modulate podosome dynamics. Biomaterials. 2015;52:494-506 pubmed publisher
  12. Lau A, Liu P, Inuzuka H, Gao D. SIRT1 phosphorylation by AMP-activated protein kinase regulates p53 acetylation. Am J Cancer Res. 2014;4:245-55 pubmed