This is a Validated Antibody Database (VAD) review about cow RPE65, based on 6 published articles (read how Labome selects the articles), using RPE65 antibody in all methods. It is aimed to help Labome visitors find the most suited RPE65 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RPE65 synonym: retinoid isomerohydrolase; all-trans-retinyl-palmitate hydrolase; meso-zeaxanthin isomerase; retinal pigment epithelium-specific 65 kDa protein; retinal pigment epithelium-specific protein (65kD); retinal pigment epithelium-specific protein 65kDa; retinol isomerase

Abcam
rabbit polyclonal
  • immunocytochemistry; human; fig 3d
  • western blot; human; loading ...; fig 3a
In order to characterize the differentiation of RPE cells, Abcam RPE65 antibody (Abcam, ab67042) was used in immunocytochemistry on human samples (fig 3d) and in western blot on human samples (fig 3a). Stem Cell Res Ther (2017) ncbi
mouse monoclonal (401.8B11.3D9)
  • western blot; mouse; loading ...; fig 4b
In order to assess the effects of N-retinyl-N-retinylidene ethanolamine on mitogen-activated protein kinase, Abcam RPE65 antibody (Abcam, ab13826) was used in western blot on mouse samples (fig 4b). Front Aging Neurosci (2017) ncbi
Novus Biologicals
mouse monoclonal (401.8B11.3D9)
  • immunohistochemistry; human; 1:200; fig 4
In order to establish an application to generate retinal pigmented epithelium from induced pluripotent stem cells, Novus Biologicals RPE65 antibody (Novus, NB100-355) was used in immunohistochemistry on human samples at 1:200 (fig 4). PLoS ONE (2017) ncbi
mouse monoclonal (401.8B11.3D9)
  • immunocytochemistry; pig; 1:50; fig 3
In order to characterize coculture model of retinal pigment epithelium cells and porcine central neuroretina explants, Novus Biologicals RPE65 antibody (Novus Biologicals, NB100-355) was used in immunocytochemistry on pig samples at 1:50 (fig 3). Mol Vis (2016) ncbi
mouse monoclonal (401.8B11.3D9)
  • immunohistochemistry - paraffin section; mouse
  • immunohistochemistry; mouse; 1:100
Novus Biologicals RPE65 antibody (Novus Biologicals, NB100-355) was used in immunohistochemistry - paraffin section on mouse samples and in immunohistochemistry on mouse samples at 1:100. Mol Vis (2014) ncbi
Invitrogen
mouse monoclonal (401.8B11.3D9)
  • immunocytochemistry; human; 1:100
In order to examine the in vitro differentiation capacity of human bone marrow-derived stem cells to develop into retinal lineages, Invitrogen RPE65 antibody (ThermoScientific, MA1-16578) was used in immunocytochemistry on human samples at 1:100. Exp Cell Res (2015) ncbi
Articles Reviewed
  1. Hazim R, Karumbayaram S, Jiang M, Dimashkie A, Lopes V, Li D, et al. Differentiation of RPE cells from integration-free iPS cells and their cell biological characterization. Stem Cell Res Ther. 2017;8:217 pubmed publisher
  2. Balmer D, Bapst Wicht L, Pyakurel A, Emery M, Nanchen N, Bochet C, et al. Bis-Retinoid A2E Induces an Increase of Basic Fibroblast Growth Factor via Inhibition of Extracellular Signal-Regulated Kinases 1/2 Pathway in Retinal Pigment Epithelium Cells and Facilitates Phagocytosis. Front Aging Neurosci. 2017;9:43 pubmed publisher
  3. Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher
  4. Di Lauro S, Rodriguez Crespo D, Gayoso M, Garcia Gutierrez M, Pastor J, Srivastava G, et al. A novel coculture model of porcine central neuroretina explants and retinal pigment epithelium cells. Mol Vis. 2016;22:243-53 pubmed
  5. Mathivanan I, Trepp C, Brunold C, Baerlocher G, Enzmann V. Retinal differentiation of human bone marrow-derived stem cells by co-culture with retinal pigment epithelium in vitro. Exp Cell Res. 2015;333:11-20 pubmed publisher
  6. Prasad T, Verma A, Li Q. Expression and cellular localization of the Mas receptor in the adult and developing mouse retina. Mol Vis. 2014;20:1443-55 pubmed