This is a Validated Antibody Database (VAD) review about cow PXN, based on 35 published articles (read how Labome selects the articles), using PXN antibody in all methods. It is aimed to help Labome visitors find the most suited PXN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PXN synonym: paxillin

Abcam
rabbit monoclonal (Y113)
  • immunohistochemistry; mouse; 1:200; loading ...; fig s1a
Abcam PXN antibody (Abcam, Y113) was used in immunohistochemistry on mouse samples at 1:200 (fig s1a). Development (2017) ncbi
rabbit monoclonal (Y113)
  • western blot; human; 1:1000; loading ...; fig 3i, 6c
Abcam PXN antibody (Abcam, ab32084) was used in western blot on human samples at 1:1000 (fig 3i, 6c). Sci Signal (2017) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:200; loading ...; fig 7A
In order to establish that Smurf1 controls Kindlin-2 protein levels in cells and hinders integrin activation, Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:200 (fig 7A). J Cell Biol (2017) ncbi
rabbit monoclonal (Y113)
  • immunohistochemistry; human; loading ...; fig 8d
  • western blot; human; loading ...; fig s8a
In order to study factors that allow tumor cells to metastasize, Abcam PXN antibody (Abcam, 32084) was used in immunohistochemistry on human samples (fig 8d) and in western blot on human samples (fig s8a). Mol Biol Cell (2017) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; mouse; loading ...; fig 3e
  • western blot; mouse; 1:300; loading ...; fig 3a
Abcam PXN antibody (Abcam, Y118) was used in immunocytochemistry on mouse samples (fig 3e) and in western blot on mouse samples at 1:300 (fig 3a). Cell Commun Signal (2017) ncbi
rabbit monoclonal (Y113)
  • western blot; human; loading ...; fig 3c
In order to assess the effects of thrombomodulin on monocyte differentiation, Abcam PXN antibody (Abcam, ab32084) was used in western blot on human samples (fig 3c). Sci Rep (2016) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human; loading ...; fig s6b
  • western blot; human; loading ...; fig s7b
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples (fig s6b) and in western blot on human samples (fig s7b). Oncotarget (2016) ncbi
rabbit monoclonal (Y113)
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Abcam PXN antibody (abcam (Epitomics), ab32084 (1500-1)) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:100; loading ...; fig 6d
In order to test if prolactin phosphorylates NEK3 to promote migration of breast cancer cells, Abcam PXN antibody (Abcam, 32084) was used in immunocytochemistry on human samples at 1:100 (fig 6d). J Biol Chem (2016) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human; fig 3a
In order to propose that ColXVII-actinin4 complexes at the lamella of a moving keratinocyte regulate actin dynamics and regulate the direction of cell movement, Abcam PXN antibody (Abcam, Y113) was used in immunocytochemistry on human samples (fig 3a). FASEB J (2016) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:200; fig 3
In order to study regulation of adhesion and cell migration by recruitment to the leading edge by human phosphatase CDC14A, Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:200 (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:2000; loading ...; fig 1b, 5
  • western blot; human; 1:5000; loading ...; fig 1d
  • immunocytochemistry; mouse; loading ...; fig 5
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:2000 (fig 1b, 5), in western blot on human samples at 1:5000 (fig 1d) and in immunocytochemistry on mouse samples (fig 5). Biophys J (2015) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:2000
  • immunocytochemistry; mouse; 1:2000
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:2000 and in immunocytochemistry on mouse samples at 1:2000. PLoS ONE (2014) ncbi
rabbit monoclonal (Y113)
  • immunocytochemistry; human
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples . Stem Cells (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (177)
  • western blot; mouse; 1:200; fig 3
Santa Cruz Biotechnology PXN antibody (santa Cruz, sc-136297) was used in western blot on mouse samples at 1:200 (fig 3). Mol Cancer (2015) ncbi
mouse monoclonal (177)
  • western blot; human; 1:1000; fig 4
In order to report that Koelreuteria formosana ethanolic extract inhibits the invasion and migration of renal cell carcinoma cells, Santa Cruz Biotechnology PXN antibody (Santa Cruz Biotechnology, sc-136297) was used in western blot on human samples at 1:1000 (fig 4). Mol Med Rep (2014) ncbi
MilliporeSigma
mouse monoclonal (PXC-10)
  • immunohistochemistry; human; loading ...; fig 8d
  • western blot; human; loading ...; fig s8a
In order to study factors that allow tumor cells to metastasize, MilliporeSigma PXN antibody (Sigma-Aldrich, P1093) was used in immunohistochemistry on human samples (fig 8d) and in western blot on human samples (fig s8a). Mol Biol Cell (2017) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig s9b
In order to examine whether p140Cap protein levels in ERBB2-amplified breast cancer patients correlate with survival, Cell Signaling Technology PXN antibody (Cell Signaling, 2542) was used in western blot on human samples at 1:1000 (fig s9b). Nat Commun (2017) ncbi
rabbit polyclonal
  • western blot; human; 1:1000; fig 6
In order to study regulation of myofiber stretch and integrin-mediated adhesion due to localized LoxL3-dependent fibronectin oxidation, Cell Signaling Technology PXN antibody (Cell Signaling, 2542) was used in western blot on human samples at 1:1000 (fig 6). Dev Cell (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 5a
In order to investigate mechanisms of tamoxifen resistance, Cell Signaling Technology PXN antibody (Cell Signaling Technology, 2542S) was used in western blot on human samples (fig 5a). Mol Cell Proteomics (2015) ncbi
rabbit polyclonal
  • western blot; human; fig 1
Cell Signaling Technology PXN antibody (Cell Signaling Technology, 2542) was used in western blot on human samples (fig 1). Nano Lett (2014) ncbi
EMD Millipore
mouse monoclonal (5H11)
  • immunocytochemistry; human; loading ...; fig s4b
EMD Millipore PXN antibody (Millipore, 05-417) was used in immunocytochemistry on human samples (fig s4b). Mol Biol Cell (2017) ncbi
mouse monoclonal (5H11)
  • immunohistochemistry; human; loading ...; fig 8d
  • western blot; human; loading ...; fig s8a
In order to study factors that allow tumor cells to metastasize, EMD Millipore PXN antibody (Millipore, 5H11) was used in immunohistochemistry on human samples (fig 8d) and in western blot on human samples (fig s8a). Mol Biol Cell (2017) ncbi
mouse monoclonal (5H11)
  • western blot; human; 1:1000; loading ...; fig 1b, 1d
In order to report that cool-associated tyrosine phosphorylated protein-1 is required for anchorage-independent growth of cervical carcinoma cells, EMD Millipore PXN antibody (Millipore, 05-417) was used in western blot on human samples at 1:1000 (fig 1b, 1d). J Biol Chem (2017) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; mouse; loading ...; fig 8
EMD Millipore PXN antibody (Millipore, 05-417) was used in immunocytochemistry on mouse samples (fig 8). J Mol Cell Cardiol (2016) ncbi
mouse monoclonal (5H11)
  • western blot; human; 1:1000; loading ...
In order to test if prolactin phosphorylates NEK3 to promote migration of breast cancer cells, EMD Millipore PXN antibody (Millipore, 05-417) was used in western blot on human samples at 1:1000. J Biol Chem (2016) ncbi
mouse monoclonal (5H11)
  • western blot; Domestic guinea pig; 1:500; fig 7
In order to assess the independence of secretion of beta-1 integrins in airway wall and focal adhesions proteins down regulation from airway hyperresponsiveness in asthma model, EMD Millipore PXN antibody (Millipore, 05-417) was used in western blot on Domestic guinea pig samples at 1:500 (fig 7). J Cell Biochem (2016) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 6c
In order to suggest that locally synthesized C1q promotes tumor growth, EMD Millipore PXN antibody (Millipore, 5H11) was used in immunocytochemistry on mouse samples at 1:100 (fig 6c). Nat Commun (2016) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; human; loading ...; fig 1h
EMD Millipore PXN antibody (Millipore, 05-417) was used in immunocytochemistry on human samples (fig 1h). Am J Physiol Lung Cell Mol Physiol (2016) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; mouse; 1:200; fig 3
  • western blot; rat; 1:1000; fig 2
In order to report that Thy-1 is a regulator of fibroblast rigidity sensing, EMD Millipore PXN antibody (Millipore, 5H11) was used in immunocytochemistry on mouse samples at 1:200 (fig 3) and in western blot on rat samples at 1:1000 (fig 2). J Cell Biol (2015) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; mouse; fig 2
In order to study stretch-activated channels to set cytosolic calcium levels controlled by transmembrane proteoglycans, EMD Millipore PXN antibody (Millipore, 5H11) was used in immunocytochemistry on mouse samples (fig 2). J Cell Biol (2015) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; human; 1:100; fig 8
In order to study the roll of cell surface heparan sulfate proteoglycans in relation to cell adhesion, EMD Millipore PXN antibody (Millipore, 05-417) was used in immunocytochemistry on human samples at 1:100 (fig 8). Mol Cancer (2015) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; mouse
EMD Millipore PXN antibody (Millipore, 05-417) was used in immunocytochemistry on mouse samples . Cell Cycle (2014) ncbi
mouse monoclonal (5H11)
  • immunohistochemistry; human; 1:500; loading ...; fig 1b
EMD Millipore PXN antibody (Millipore, 05-417) was used in immunohistochemistry on human samples at 1:500 (fig 1b). Nat Commun (2014) ncbi
mouse monoclonal (5H11)
  • western blot; human
EMD Millipore PXN antibody (Millipore, 05-417) was used in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; mouse; 5-10 mg/ml
EMD Millipore PXN antibody (Millipore, 5H11) was used in immunocytochemistry on mouse samples at 5-10 mg/ml. FASEB J (2014) ncbi
mouse monoclonal (5H11)
  • immunocytochemistry; human
EMD Millipore PXN antibody (Millipore, 05-417) was used in immunocytochemistry on human samples . PLoS ONE (2013) ncbi
mouse monoclonal (5H11)
  • western blot; human
EMD Millipore PXN antibody (Millipore, 5H11) was used in western blot on human samples . Brain (2013) ncbi
Articles Reviewed
  1. Rashid M, Belmont J, Carpenter D, Turner C, Olson E. Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation. Development. 2017;144:4002-4014 pubmed publisher
  2. Amin E, Liu Y, Deng S, Tan K, Chudgar N, Mayo M, et al. The RNA-editing enzyme ADAR promotes lung adenocarcinoma migration and invasion by stabilizing FAK. Sci Signal. 2017;10: pubmed publisher
  3. Owen L, Adhikari A, Patel M, Grimmer P, Leijnse N, Kim M, et al. A cytoskeletal clutch mediates cellular force transmission in a soft, three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1959-1974 pubmed publisher
  4. Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
  5. Mekhdjian A, Kai F, Rubashkin M, Prahl L, Przybyla L, McGregor A, et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1467-1488 pubmed publisher
  6. Grasso S, Chapelle J, Salemme V, Aramu S, Russo I, Vitale N, et al. The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries. Nat Commun. 2017;8:14797 pubmed publisher
  7. Yoo S, Latifkar A, Cerione R, Antonyak M. Cool-associated Tyrosine-phosphorylated Protein 1 Is Required for the Anchorage-independent Growth of Cervical Carcinoma Cells by Binding Paxillin and Promoting AKT Activation. J Biol Chem. 2017;292:3947-3957 pubmed publisher
  8. Shen X, Jia Z, D Alonzo D, Wang X, Bruder E, Emch F, et al. HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures. Cell Commun Signal. 2017;15:2 pubmed publisher
  9. Tsai C, Lin Y, Huang C, Shih C, Tsai Y, Tsao N, et al. Thrombomodulin regulates monocye differentiation via PKC? and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep. 2016;6:38421 pubmed publisher
  10. Ross Adams H, Ball S, Lawrenson K, Halim S, Russell R, Wells C, et al. HNF1B variants associate with promoter methylation and regulate gene networks activated in prostate and ovarian cancer. Oncotarget. 2016;7:74734-74746 pubmed publisher
  11. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  12. Liu R, Jin J. Deletion of calponin 2 in macrophages alters cytoskeleton-based functions and attenuates the development of atherosclerosis. J Mol Cell Cardiol. 2016;99:87-99 pubmed publisher
  13. Harrington K, Clevenger C. Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration. J Biol Chem. 2016;291:21388-21406 pubmed
  14. Álvarez Santos M, Carbajal V, Tellez Jiménez O, Martínez Cordero E, Ruiz V, Hernández Pando R, et al. Airway Hyperresponsiveness in Asthma Model Occurs Independently of Secretion of β1 Integrins in Airway Wall and Focal Adhesions Proteins Down Regulation. J Cell Biochem. 2016;117:2385-96 pubmed publisher
  15. Kraft Sheleg O, Zaffryar Eilot S, Genin O, Yaseen W, Soueid Baumgarten S, Kessler O, et al. Localized LoxL3-Dependent Fibronectin Oxidation Regulates Myofiber Stretch and Integrin-Mediated Adhesion. Dev Cell. 2016;36:550-61 pubmed publisher
  16. Hiroyasu S, Colburn Z, Jones J. A hemidesmosomal protein regulates actin dynamics and traction forces in motile keratinocytes. FASEB J. 2016;30:2298-310 pubmed publisher
  17. Bulla R, Tripodo C, Rami D, Ling G, Agostinis C, Guarnotta C, et al. C1q acts in the tumour microenvironment as a cancer-promoting factor independently of complement activation. Nat Commun. 2016;7:10346 pubmed publisher
  18. Chen N, Uddin B, Voit R, Schiebel E. Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion. Proc Natl Acad Sci U S A. 2016;113:990-5 pubmed publisher
  19. Awad K, Elinoff J, Wang S, Gairhe S, Ferreyra G, Cai R, et al. Raf/ERK drives the proliferative and invasive phenotype of BMPR2-silenced pulmonary artery endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2016;310:L187-201 pubmed publisher
  20. Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
  21. Gopal S, Søgaard P, Multhaupt H, Pataki C, Okina E, Xian X, et al. Transmembrane proteoglycans control stretch-activated channels to set cytosolic calcium levels. J Cell Biol. 2015;210:1199-211 pubmed publisher
  22. Darr J, Klochendler A, Isaac S, Geiger T, Geiger T, Eden A. Phosphoproteomic analysis reveals Smarcb1 dependent EGFR signaling in Malignant Rhabdoid tumor cells. Mol Cancer. 2015;14:167 pubmed publisher
  23. Wu X, Zahari M, Renuse S, Nirujogi R, Kim M, Manda S, et al. Phosphoproteomic Analysis Identifies Focal Adhesion Kinase 2 (FAK2) as a Potential Therapeutic Target for Tamoxifen Resistance in Breast Cancer. Mol Cell Proteomics. 2015;14:2887-900 pubmed publisher
  24. Fuhrmann A, Engler A. The cytoskeleton regulates cell attachment strength. Biophys J. 2015;109:57-65 pubmed publisher
  25. Lim H, Multhaupt H, Couchman J. Cell surface heparan sulfate proteoglycans control adhesion and invasion of breast carcinoma cells. Mol Cancer. 2015;14:15 pubmed publisher
  26. Hoon J, Li H, Koh C. POPX2 phosphatase regulates cell polarity and centrosome placement. Cell Cycle. 2014;13:2459-68 pubmed publisher
  27. Lin C, Chen P, Hsu L, Kuo D, Chu S, Hsieh Y. Inhibition of the invasion and migration of renal carcinoma 786‑o‑si3 cells in vitro and in vivo by Koelreuteria formosana extract. Mol Med Rep. 2014;10:3334-42 pubmed publisher
  28. Fuhrmann A, Li J, Chien S, Engler A. Cation type specific cell remodeling regulates attachment strength. PLoS ONE. 2014;9:e102424 pubmed publisher
  29. Majeed S, Vasudevan L, Chen C, Luo Y, Torres J, Evans T, et al. Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. Nat Commun. 2014;5:3891 pubmed publisher
  30. Chen Q, Xu R, Zeng C, Lu Q, Huang D, Shi C, et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ?4-mediated FAK signaling. PLoS ONE. 2014;9:e88386 pubmed publisher
  31. Potthoff E, Franco D, D Alessandro V, Starck C, Falk V, Zambelli T, et al. Toward a rational design of surface textures promoting endothelialization. Nano Lett. 2014;14:1069-79 pubmed publisher
  32. Wu T, Kooi C, Shah P, Charnigo R, Huang C, Smyth S, et al. Integrin-mediated cell surface recruitment of autotaxin promotes persistent directional cell migration. FASEB J. 2014;28:861-70 pubmed publisher
  33. Holle A, Tang X, Vijayraghavan D, Vincent L, Fuhrmann A, Choi Y, et al. In situ mechanotransduction via vinculin regulates stem cell differentiation. Stem Cells. 2013;31:2467-77 pubmed publisher
  34. Au S, Wong C, Lee J, Wong C, Ng I. EZH2-Mediated H3K27me3 Is Involved in Epigenetic Repression of Deleted in Liver Cancer 1 in Human Cancers. PLoS ONE. 2013;8:e68226 pubmed publisher
  35. Doddrell R, Dun X, Shivane A, Feltri M, Wrabetz L, Wegner M, et al. Loss of SOX10 function contributes to the phenotype of human Merlin-null schwannoma cells. Brain. 2013;136:549-63 pubmed publisher