This is a Validated Antibody Database (VAD) review about cow PPP2CA, based on 28 published articles (read how Labome selects the articles), using PPP2CA antibody in all methods. It is aimed to help Labome visitors find the most suited PPP2CA antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PPP2CA synonym: serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform; PP2A-alpha; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform

EMD Millipore
mouse monoclonal (1D6)
  • western blot; human; loading ...; fig 2d
EMD Millipore PPP2CA antibody (merck, 05-421) was used in western blot on human samples (fig 2d). Nat Cell Biol (2017) ncbi
mouse monoclonal (1D6)
  • western blot; mouse; fig s3
In order to explore the role of Fancd2 in normal development and tumorigenesis, EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on mouse samples (fig s3). Sci Rep (2017) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; human
  • western blot; human; loading ...; fig 2l
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in immunoprecipitation on human samples and in western blot on human samples (fig 2l). Oncotarget (2016) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; rat; loading ...; fig 2a
  • immunocytochemistry; rat; 1:200; loading ...; fig 4a
  • western blot; rat; 1:2000; loading ...; fig 2a
EMD Millipore PPP2CA antibody (Millipore, 1D6) was used in immunoprecipitation on rat samples (fig 2a), in immunocytochemistry on rat samples at 1:200 (fig 4a) and in western blot on rat samples at 1:2000 (fig 2a). J Neurochem (2016) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; human; fig 4
  • western blot; human; fig 4
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in immunoprecipitation on human samples (fig 4) and in western blot on human samples (fig 4). Sci Rep (2016) ncbi
mouse monoclonal (1D6)
  • western blot; rat; 1:2000; fig s1
In order to investigate left ventricular cardiomyocytes from post-infaction rats with and without heart failure after beta-adrenoceptor stimulation to revela Ca2+ waves and sarcoplasmic reticulum Ca2+ depletion, EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on rat samples at 1:2000 (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; human; fig s3
  • western blot; human; fig s3
  • western blot; mouse; fig 3
In order to study the requisite for the function of regulatory T cells known as phosphatase PP2A, EMD Millipore PPP2CA antibody (Millipore, 1D6) was used in immunoprecipitation on human samples (fig s3), in western blot on human samples (fig s3) and in western blot on mouse samples (fig 3). Nat Immunol (2016) ncbi
mouse monoclonal (1D6)
  • western blot; human; 1:2500; fig 2
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on human samples at 1:2500 (fig 2). Nat Commun (2016) ncbi
mouse monoclonal (1D6)
  • western blot; human; fig 5
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on human samples (fig 5). Cell Mol Life Sci (2016) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 2). Cell Cycle (2016) ncbi
mouse monoclonal (1D6)
  • western blot; human; fig 6
EMD Millipore PPP2CA antibody (Merck Millipore, 1D6) was used in western blot on human samples (fig 6). Sci Rep (2016) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; mouse; fig s6
EMD Millipore PPP2CA antibody (Merck Millipore, 05-421) was used in immunoprecipitation on mouse samples (fig s6). PLoS ONE (2015) ncbi
rabbit polyclonal
  • western blot; mouse; fig 5
EMD Millipore PPP2CA antibody (Merck Millipore, 07-324) was used in western blot on mouse samples (fig 5). PLoS ONE (2015) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; mouse; fig s9
EMD Millipore PPP2CA antibody (Upstate Biotechnology, 05-421) was used in immunoprecipitation on mouse samples (fig s9). Sci Rep (2015) ncbi
mouse monoclonal (1D6)
  • western blot; human
In order to study the effect of MASTL on cyclin B1 degradation and its mechanism, EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on human samples . Biol Open (2015) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; human; fig 1
  • western blot; human; fig 2
EMD Millipore PPP2CA antibody (Millipore, 1D6) was used in immunoprecipitation on human samples (fig 1) and in western blot on human samples (fig 2). J Virol (2015) ncbi
mouse monoclonal (1D6)
  • western blot; mouse; fig 3
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on mouse samples (fig 3). Front Aging Neurosci (2014) ncbi
mouse monoclonal (1D6)
  • western blot; mouse; 1:2000
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on mouse samples at 1:2000. BMC Neurosci (2014) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; mouse; 4 ug
EMD Millipore PPP2CA antibody (Upstate, 1D6) was used in immunoprecipitation on mouse samples at 4 ug. Mol Cell Biol (2014) ncbi
rabbit polyclonal
  • western blot; dog
EMD Millipore PPP2CA antibody (Millipore, 07-324) was used in western blot on dog samples . J Vet Med Sci (2014) ncbi
mouse monoclonal (1D6)
  • western blot; common platanna; 0.2 ug/ml
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on common platanna samples at 0.2 ug/ml. J Biol Chem (2014) ncbi
mouse monoclonal (1D6)
  • western blot; human
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on human samples . Br J Pharmacol (2014) ncbi
mouse monoclonal (1D6)
  • western blot; green monkey; 1:1000
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on green monkey samples at 1:1000. J Virol (2014) ncbi
mouse monoclonal (1D6)
  • western blot; mouse; 1:800
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on mouse samples at 1:800. PLoS ONE (2014) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; rat
  • western blot; rat
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in immunoprecipitation on rat samples and in western blot on rat samples . PLoS ONE (2014) ncbi
mouse monoclonal (1D6)
  • western blot; mouse; 1:500
In order to study the interaction between F-box protein FBXL16 and PP2A-B55alpha and its effect on the differentiation of embryonic stem cells, EMD Millipore PPP2CA antibody (Millipore, 05?C421) was used in western blot on mouse samples at 1:500. Mol Cell Proteomics (2014) ncbi
mouse monoclonal (1D6)
  • immunoprecipitation; human
  • western blot; human
EMD Millipore PPP2CA antibody (Millipore, 05421) was used in immunoprecipitation on human samples and in western blot on human samples . Mol Cancer Ther (2013) ncbi
mouse monoclonal (1D6)
  • western blot; human
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on human samples . Nucleic Acids Res (2013) ncbi
mouse monoclonal (1D6)
  • western blot; rat; 1:500
  • immunocytochemistry; mouse
  • western blot; mouse; 1:500
  • western blot; dog; 1:500
  • western blot; human; 1:500
EMD Millipore PPP2CA antibody (Millipore, 05-421) was used in western blot on rat samples at 1:500, in immunocytochemistry on mouse samples , in western blot on mouse samples at 1:500, in western blot on dog samples at 1:500 and in western blot on human samples at 1:500. J Biol Chem (2013) ncbi
Articles Reviewed
  1. Hein J, Hertz E, Garvanska D, Kruse T, Nilsson J. Distinct kinetics of serine and threonine dephosphorylation are essential for mitosis. Nat Cell Biol. 2017;19:1433-1440 pubmed publisher
  2. Zhang T, Du W, Wilson A, Namekawa S, Andreassen P, Meetei A, et al. Fancd2 in vivo interaction network reveals a non-canonical role in mitochondrial function. Sci Rep. 2017;7:45626 pubmed publisher
  3. Fan L, Liu M, Guo M, Hu C, Yan Z, Chen J, et al. FAM122A, a new endogenous inhibitor of protein phosphatase 2A. Oncotarget. 2016;7:63887-63900 pubmed publisher
  4. Juraszek B, Nałęcz K. Protein phosphatase PP2A - a novel interacting partner of carnitine transporter OCTN2 (SLC22A5) in rat astrocytes. J Neurochem. 2016;139:537-551 pubmed publisher
  5. Jeong A, Han S, Lee S, Su Park J, Lu Y, Yu S, et al. Patient derived mutation W257G of PPP2R1A enhances cancer cell migration through SRC-JNK-c-Jun pathway. Sci Rep. 2016;6:27391 pubmed publisher
  6. Sadredini M, Danielsen T, Aronsen J, Manotheepan R, Hougen K, Sjaastad I, et al. Beta-Adrenoceptor Stimulation Reveals Ca2+ Waves and Sarcoplasmic Reticulum Ca2+ Depletion in Left Ventricular Cardiomyocytes from Post-Infarction Rats with and without Heart Failure. PLoS ONE. 2016;11:e0153887 pubmed publisher
  7. Apostolidis S, Rodríguez Rodríguez N, Suárez Fueyo A, Dioufa N, Ozcan E, Crispín J, et al. Phosphatase PP2A is requisite for the function of regulatory T cells. Nat Immunol. 2016;17:556-64 pubmed publisher
  8. Hein J, Nilsson J. Interphase APC/C-Cdc20 inhibition by cyclin A2-Cdk2 ensures efficient mitotic entry. Nat Commun. 2016;7:10975 pubmed publisher
  9. Lee S, Jeong A, Park J, Han S, Jang C, Kim K, et al. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cell Mol Life Sci. 2016;73:3375-86 pubmed publisher
  10. Katoh I, Fukunishi N, Fujimuro M, Kasai H, Moriishi K, Hata R, et al. Repression of Wnt/β-catenin response elements by p63 (TP63). Cell Cycle. 2016;15:699-710 pubmed publisher
  11. Asano Y, Kawase T, Okabe A, Tsutsumi S, Ichikawa H, Tatebe S, et al. IER5 generates a novel hypo-phosphorylated active form of HSF1 and contributes to tumorigenesis. Sci Rep. 2016;6:19174 pubmed publisher
  12. Yabe R, Miura A, Usui T, Mudrak I, Ogris E, Ohama T, et al. Protein Phosphatase Methyl-Esterase PME-1 Protects Protein Phosphatase 2A from Ubiquitin/Proteasome Degradation. PLoS ONE. 2015;10:e0145226 pubmed publisher
  13. Hu X, Garcia C, Fazli L, Gleave M, Vitek M, Jansen M, et al. Inhibition of Pten deficient Castration Resistant Prostate Cancer by Targeting of the SET - PP2A Signaling axis. Sci Rep. 2015;5:15182 pubmed publisher
  14. Voets E, Wolthuis R. MASTL promotes cyclin B1 destruction by enforcing Cdc20-independent binding of cyclin B1 to the APC/C. Biol Open. 2015;4:484-95 pubmed publisher
  15. Kwun H, Shuda M, Camacho C, Gamper A, Thant M, Chang Y, et al. Restricted protein phosphatase 2A targeting by Merkel cell polyomavirus small T antigen. J Virol. 2015;89:4191-200 pubmed publisher
  16. Sontag J, Wasek B, Taleski G, Smith J, Arning E, Sontag E, et al. Altered protein phosphatase 2A methylation and Tau phosphorylation in the young and aged brain of methylenetetrahydrofolate reductase (MTHFR) deficient mice. Front Aging Neurosci. 2014;6:214 pubmed publisher
  17. Chasseigneaux S, Clamagirand C, Huguet L, Gorisse Hussonnois L, Rose C, Allinquant B. Cytoplasmic SET induces tau hyperphosphorylation through a decrease of methylated phosphatase 2A. BMC Neurosci. 2014;15:82 pubmed publisher
  18. Mamouni K, Cristini A, Guirouilh Barbat J, Monferran S, Lemarie A, Faye J, et al. RhoB promotes ?H2AX dephosphorylation and DNA double-strand break repair. Mol Cell Biol. 2014;34:3144-55 pubmed publisher
  19. Yabe R, Fujiwara N, Mizuno T, Usui T, Ohama T, Sato K. Characterization of SET/I2PP2A isoforms in dogs. J Vet Med Sci. 2014;76:1235-40 pubmed
  20. Kang Q, Srividhya J, Ipe J, Pomerening J. Evidence toward a dual phosphatase mechanism that restricts Aurora A (Thr-295) phosphorylation during the early embryonic cell cycle. J Biol Chem. 2014;289:17480-96 pubmed publisher
  21. Dou X, Xia Y, Chen J, Qian Y, Li S, Zhang X, et al. Rectification of impaired adipose tissue methylation status and lipolytic response contributes to hepatoprotective effect of betaine in a mouse model of alcoholic liver disease. Br J Pharmacol. 2014;171:4073-86 pubmed publisher
  22. Jamin A, Wicklund A, Wiebe M. Cell- and virus-mediated regulation of the barrier-to-autointegration factor's phosphorylation state controls its DNA binding, dimerization, subcellular localization, and antipoxviral activity. J Virol. 2014;88:5342-55 pubmed publisher
  23. Perera N, Sheean R, Scott J, Kemp B, Horne M, Turner B. Mutant TDP-43 deregulates AMPK activation by PP2A in ALS models. PLoS ONE. 2014;9:e90449 pubmed publisher
  24. Longman M, Ranieri A, Avkiran M, Snabaitis A. Regulation of PP2AC carboxylmethylation and cellular localisation by inhibitory class G-protein coupled receptors in cardiomyocytes. PLoS ONE. 2014;9:e86234 pubmed publisher
  25. Honarpour N, Rose C, Brumbaugh J, Anderson J, Graham R, Sweredoski M, et al. F-box protein FBXL16 binds PP2A-B55? and regulates differentiation of embryonic stem cells along the FLK1+ lineage. Mol Cell Proteomics. 2014;13:780-91 pubmed publisher
  26. Sreevalsan S, Safe S. The cannabinoid WIN 55,212-2 decreases specificity protein transcription factors and the oncogenic cap protein eIF4E in colon cancer cells. Mol Cancer Ther. 2013;12:2483-93 pubmed publisher
  27. Arampatzi P, Gialitakis M, Makatounakis T, Papamatheakis J. Gene-specific factors determine mitotic expression and bookmarking via alternate regulatory elements. Nucleic Acids Res. 2013;41:2202-15 pubmed publisher
  28. Degrande S, Little S, Nixon D, Wright P, Snyder J, Dun W, et al. Molecular mechanisms underlying cardiac protein phosphatase 2A regulation in heart. J Biol Chem. 2013;288:1032-46 pubmed publisher