This is a Validated Antibody Database (VAD) review about cow POLR2A, based on 38 published articles (read how Labome selects the articles), using POLR2A antibody in all methods. It is aimed to help Labome visitors find the most suited POLR2A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
POLR2A synonym: DNA-directed RNA polymerase II subunit RPB1; polymerase (RNA) II (DNA directed) polypeptide A (220kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa

Abcam
mouse monoclonal (8WG16)
  • ChIP-Seq; human; fig 3a
Abcam POLR2A antibody (Abcam, ab817) was used in ChIP-Seq on human samples (fig 3a). Cell (2018) ncbi
mouse monoclonal (8WG16)
  • immunocytochemistry; mouse; 1:200; loading ...; fig 1b
Abcam POLR2A antibody (Abcam, 8WG16) was used in immunocytochemistry on mouse samples at 1:200 (fig 1b). Methods Mol Biol (2017) ncbi
mouse monoclonal (8WG16)
  • western blot; human; loading ...; fig 4b
Abcam POLR2A antibody (Abcam, ab817) was used in western blot on human samples (fig 4b). Nature (2017) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human; fig s5d
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples (fig s5d). PLoS Genet (2017) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; mouse; fig 4e
In order to characterize a int-1-GATA knockout mouse, Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on mouse samples (fig 4e). Nucleic Acids Res (2017) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human; fig 6
Abcam POLR2A antibody (Abcam, Ab-817) was used in chromatin immunoprecipitation on human samples (fig 6). Nat Commun (2016) ncbi
mouse monoclonal (8WG16)
  • western blot; human; loading ...; fig 2d
Abcam POLR2A antibody (Abcam, ab817) was used in western blot on human samples (fig 2d). Biochemistry (2016) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human; fig 3
In order to characterize regulation of PD-L1 in melanoma by HDAC6, Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples (fig 3). Mol Oncol (2016) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human; fig 4
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples (fig 4). Mol Cancer (2015) ncbi
mouse monoclonal (8WG16)
  • ChIP-Seq; thale cress; fig s4a
Abcam POLR2A antibody (Abcam, ab817) was used in ChIP-Seq on thale cress samples (fig s4a). Cell (2015) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human; fig 3d
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples (fig 3d). Nature (2015) ncbi
mouse monoclonal (8WG16)
  • ChIP-Seq; human; fig 6
  • ChIP-Seq; fission yeast; fig 2
  • western blot; fission yeast; 1:2000; fig s5
Abcam POLR2A antibody (abcam, 8WG16) was used in ChIP-Seq on human samples (fig 6), in ChIP-Seq on fission yeast samples (fig 2) and in western blot on fission yeast samples at 1:2000 (fig s5). Nat Commun (2015) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human; fig 6
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples (fig 6). Mol Cell Biol (2015) ncbi
goat polyclonal
  • western blot; human; fig s3d
Abcam POLR2A antibody (Abcam, ab140509) was used in western blot on human samples (fig s3d). Nature (2015) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human; loading ...; fig 4c
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples (fig 4c). Virol J (2015) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples . Oncogene (2015) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; fruit fly; loading ...; fig 6c
  • western blot; fruit fly; 1:500; loading ...; fig 6b
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on fruit fly samples (fig 6c) and in western blot on fruit fly samples at 1:500 (fig 6b). PLoS Genet (2015) ncbi
mouse monoclonal (8WG16)
Abcam POLR2A antibody (abcam, ab817) was used . Cell Cycle (2016) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples . Nucleic Acids Res (2014) ncbi
mouse monoclonal (8WG16)
  • ChIP-Seq; human
Abcam POLR2A antibody (Abcam, ab817) was used in ChIP-Seq on human samples . Nucleic Acids Res (2014) ncbi
mouse monoclonal (8WG16)
  • western blot; budding yeasts
Abcam POLR2A antibody (Abcam, 8WG16) was used in western blot on budding yeasts samples . J Biol Chem (2014) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; mouse
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on mouse samples . PLoS ONE (2014) ncbi
mouse monoclonal (8WG16)
  • immunohistochemistry; thale cress; 1:100
In order to discuss histone H3 variants in plants and animals, Abcam POLR2A antibody (Abcam, ab817) was used in immunohistochemistry on thale cress samples at 1:100. Genome Biol (2014) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples . Physiol Genomics (2013) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human
In order to report a tumor-suppressive role of NOV in prostate cancer, Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on human samples . Oncogene (2014) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; mouse
  • chromatin immunoprecipitation; human
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on mouse samples and in chromatin immunoprecipitation on human samples . Mol Biol Cell (2013) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; mouse
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on mouse samples . J Immunol (2011) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; Caenorhabditis elegans; 2-5 ug
Abcam POLR2A antibody (Abcam, ab817) was used in chromatin immunoprecipitation on Caenorhabditis elegans samples at 2-5 ug. PLoS Genet (2010) ncbi
Santa Cruz Biotechnology
mouse monoclonal (8WG16)
  • immunoprecipitation; human; loading ...; fig 3c
  • western blot; human; loading ...; fig 3a
Santa Cruz Biotechnology POLR2A antibody (Santa Cruz Biotechnology, sc-56767) was used in immunoprecipitation on human samples (fig 3c) and in western blot on human samples (fig 3a). Nucleic Acids Res (2019) ncbi
mouse monoclonal (8WG16)
  • immunoprecipitation; human; loading ...; fig 4h
Santa Cruz Biotechnology POLR2A antibody (Santa Cruz Biotechnology, sc-56767) was used in immunoprecipitation on human samples (fig 4h). Nucleic Acids Res (2017) ncbi
mouse monoclonal (8WG16)
  • immunohistochemistry; mouse; loading ...; fig s2a
In order to demonstrate that some mitochondrial enzymes associated with the tricarboxylic acid cycle are essential for epigenetic remodeling and transiently localize to the nucleus, Santa Cruz Biotechnology POLR2A antibody (Santa Cruz, 8WG16) was used in immunohistochemistry on mouse samples (fig s2a). Cell (2017) ncbi
mouse monoclonal (8WG16)
  • western blot; mouse; fig 7
In order to characterize the involvement of concerted non-productive splicing of post-transcriptional regulators that involves the alternative splicing program of differentiatied smooth muscle cells, Santa Cruz Biotechnology POLR2A antibody (Santa Cruz Biotechnology, sc-56767) was used in western blot on mouse samples (fig 7). Nucleic Acids Res (2016) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; mouse
In order to demonstrate that miR-200 suppresses TGF-beta/BMP signaling, promotes epithelial gene expression, and suppresses cell invasion by regulating a network of genes, Santa Cruz Biotechnology POLR2A antibody (Santa Cruz, sc-56767) was used in chromatin immunoprecipitation on mouse samples . Oncogene (2016) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; human
Santa Cruz Biotechnology POLR2A antibody (Santa Cruz, sc-56767) was used in chromatin immunoprecipitation on human samples . Breast Cancer Res Treat (2015) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; mouse
Santa Cruz Biotechnology POLR2A antibody (Santa Cruz Biotechnologies, sc-56767) was used in chromatin immunoprecipitation on mouse samples . PLoS ONE (2014) ncbi
mouse monoclonal (8WG16)
  • immunoprecipitation; human
  • western blot; human
Santa Cruz Biotechnology POLR2A antibody (Santa Cruz Biotech, sc-56767) was used in immunoprecipitation on human samples and in western blot on human samples . Nucleic Acids Res (2014) ncbi
Invitrogen
mouse monoclonal (8WG16)
  • western blot; fission yeast; loading ...; fig 3b
In order to discover a role for RNA-DNA hybrids and RNase H enzymes in DNA repair, Invitrogen POLR2A antibody (Thermo Fischer, MA1-26249) was used in western blot on fission yeast samples (fig 3b). Cell (2016) ncbi
mouse monoclonal (8WG16)
  • chromatin immunoprecipitation; rat
Invitrogen POLR2A antibody (Pierce, 8WG16) was used in chromatin immunoprecipitation on rat samples . Am J Physiol Regul Integr Comp Physiol (2015) ncbi
Articles Reviewed
  1. Yu D, Cattoglio C, Xue Y, Zhou Q. A complex between DYRK1A and DCAF7 phosphorylates the C-terminal domain of RNA polymerase II to promote myogenesis. Nucleic Acids Res. 2019;47:4462-4475 pubmed publisher
  2. Liu X, Wu H, Krzisch M, Wu X, Graef J, Muffat J, et al. Rescue of Fragile X Syndrome Neurons by DNA Methylation Editing of the FMR1 Gene. Cell. 2018;172:979-992.e6 pubmed publisher
  3. Bogolyubova I, Bogolyubov D. Detection of RNA Polymerase II in Mouse Embryos During Zygotic Genome Activation Using Immunocytochemistry. Methods Mol Biol. 2017;1605:147-159 pubmed publisher
  4. Blank M, Chen S, Poetz F, Schnolzer M, Voit R, Grummt I. SIRT7-dependent deacetylation of CDK9 activates RNA polymerase II transcription. Nucleic Acids Res. 2017;45:2675-2686 pubmed publisher
  5. Aktas T, Avşar Ilık İ, Maticzka D, Bhardwaj V, Pessoa Rodrigues C, Mittler G, et al. DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome. Nature. 2017;544:115-119 pubmed publisher
  6. Shadle S, Zhong J, Campbell A, Conerly M, Jagannathan S, Wong C, et al. DUX4-induced dsRNA and MYC mRNA stabilization activate apoptotic pathways in human cell models of facioscapulohumeral dystrophy. PLoS Genet. 2017;13:e1006658 pubmed publisher
  7. Zhang Y, Zhang J, An W, Wan Y, Ma S, Yin J, et al. Intron 1 GATA site enhances ALAS2 expression indispensably during erythroid differentiation. Nucleic Acids Res. 2017;45:657-671 pubmed publisher
  8. Nagaraj R, Sharpley M, Chi F, Braas D, Zhou Y, Kim R, et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell. 2017;168:210-223.e11 pubmed publisher
  9. Ohle C, Tesorero R, Schermann G, Dobrev N, Sinning I, Fischer T. Transient RNA-DNA Hybrids Are Required for Efficient Double-Strand Break Repair. Cell. 2016;167:1001-1013.e7 pubmed publisher
  10. Llorian M, Gooding C, Bellora N, Hallegger M, Buckroyd A, Wang X, et al. The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators. Nucleic Acids Res. 2016;44:8933-8950 pubmed
  11. Diamant G, Bahat A, Dikstein R. The elongation factor Spt5 facilitates transcription initiation for rapid induction of inflammatory-response genes. Nat Commun. 2016;7:11547 pubmed publisher
  12. Lu L, Fan D, Hu C, Worth M, Ma Z, Jiang J. Distributive O-GlcNAcylation on the Highly Repetitive C-Terminal Domain of RNA Polymerase II. Biochemistry. 2016;55:1149-58 pubmed publisher
  13. M L, P P, T K, M P, E S, J P, et al. Essential role of HDAC6 in the regulation of PD-L1 in melanoma. Mol Oncol. 2016;10:735-750 pubmed publisher
  14. Kreuz S, Holmes K, Tooze R, Lefevre P. Loss of PIM2 enhances the anti-proliferative effect of the pan-PIM kinase inhibitor AZD1208 in non-Hodgkin lymphomas. Mol Cancer. 2015;14:205 pubmed publisher
  15. Zhai J, Bischof S, Wang H, Feng S, Lee T, Teng C, et al. A One Precursor One siRNA Model for Pol IV-Dependent siRNA Biogenesis. Cell. 2015;163:445-55 pubmed publisher
  16. Zhu J, Sammons M, Donahue G, Dou Z, Vedadi M, Getlik M, et al. Gain-of-function p53 mutants co-opt chromatin pathways to drive cancer growth. Nature. 2015;525:206-11 pubmed publisher
  17. Sutani T, Sakata T, Nakato R, Masuda K, Ishibashi M, Yamashita D, et al. Condensin targets and reduces unwound DNA structures associated with transcription in mitotic chromosome condensation. Nat Commun. 2015;6:7815 pubmed publisher
  18. Eid R, Demattei M, Episkopou H, Augé Gouillou C, Decottignies A, Grandin N, et al. Genetic Inactivation of ATRX Leads to a Decrease in the Amount of Telomeric Cohesin and Level of Telomere Transcription in Human Glioma Cells. Mol Cell Biol. 2015;35:2818-30 pubmed publisher
  19. Liu Y, Zhang X, Han C, Wan G, Huang X, Ivan C, et al. TP53 loss creates therapeutic vulnerability in colorectal cancer. Nature. 2015;520:697-701 pubmed publisher
  20. Guendel I, Meltzer B, Baer A, Dever S, Valerie K, Guo J, et al. BRCA1 functions as a novel transcriptional cofactor in HIV-1 infection. Virol J. 2015;12:40 pubmed publisher
  21. Perdigão Henriques R, Petrocca F, Altschuler G, Thomas M, Le M, Tan S, et al. miR-200 promotes the mesenchymal to epithelial transition by suppressing multiple members of the Zeb2 and Snail1 transcriptional repressor complexes. Oncogene. 2016;35:158-72 pubmed publisher
  22. Jiang Y, Yan B, Lai W, Shi Y, Xiao D, Jia J, et al. Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene. 2015;34:6079-91 pubmed publisher
  23. Sengupta S, Biarnes M, Clarke R, Jordan V. Inhibition of BET proteins impairs estrogen-mediated growth and transcription in breast cancers by pausing RNA polymerase advancement. Breast Cancer Res Treat. 2015;150:265-78 pubmed publisher
  24. Dahlberg O, Shilkova O, Tang M, Holmqvist P, Mannervik M. P-TEFb, the super elongation complex and mediator regulate a subset of non-paused genes during early Drosophila embryo development. PLoS Genet. 2015;11:e1004971 pubmed publisher
  25. Tran P, Kennedy B, Lien Y, Simmons R, Georgieff M. Fetal iron deficiency induces chromatin remodeling at the Bdnf locus in adult rat hippocampus. Am J Physiol Regul Integr Comp Physiol. 2015;308:R276-82 pubmed publisher
  26. Agarwal P, Enroth S, Teichmann M, Jernberg Wiklund H, Smit A, Westermark B, et al. Growth signals employ CGGBP1 to suppress transcription of Alu-SINEs. Cell Cycle. 2016;15:1558-71 pubmed publisher
  27. Suzuki A, Makinoshima H, Wakaguri H, Esumi H, Sugano S, Kohno T, et al. Aberrant transcriptional regulations in cancers: genome, transcriptome and epigenome analysis of lung adenocarcinoma cell lines. Nucleic Acids Res. 2014;42:13557-72 pubmed publisher
  28. Matsumoto K, Suzuki A, Wakaguri H, Sugano S, Suzuki Y. Construction of mate pair full-length cDNAs libraries and characterization of transcriptional start sites and termination sites. Nucleic Acids Res. 2014;42:e125 pubmed publisher
  29. Schulz D, Pirkl N, Lehmann E, Cramer P. Rpb4 subunit functions mainly in mRNA synthesis by RNA polymerase II. J Biol Chem. 2014;289:17446-52 pubmed publisher
  30. Seki M, Masaki H, Arauchi T, Nakauchi H, Sugano S, Suzuki Y. A comparison of the rest complex binding patterns in embryonic stem cells and epiblast stem cells. PLoS ONE. 2014;9:e95374 pubmed publisher
  31. Shu H, Nakamura M, Siretskiy A, Borghi L, Moraes I, Wildhaber T, et al. Arabidopsis replacement histone variant H3.3 occupies promoters of regulated genes. Genome Biol. 2014;15:R62 pubmed publisher
  32. Gjidoda A, Tagore M, McAndrew M, Woods A, Floer M. Nucleosomes are stably evicted from enhancers but not promoters upon induction of certain pro-inflammatory genes in mouse macrophages. PLoS ONE. 2014;9:e93971 pubmed publisher
  33. Morales J, Richard P, Rommel A, Fattah F, Motea E, Patidar P, et al. Kub5-Hera, the human Rtt103 homolog, plays dual functional roles in transcription termination and DNA repair. Nucleic Acids Res. 2014;42:4996-5006 pubmed publisher
  34. Miao F, Chen Z, Zhang L, Wang J, Gao H, Wu X, et al. RNA-sequencing analysis of high glucose-treated monocytes reveals novel transcriptome signatures and associated epigenetic profiles. Physiol Genomics. 2013;45:287-99 pubmed publisher
  35. Wu L, Runkle C, Jin H, Li J, Yang X, Kuzel T, et al. CCN3/NOV gene expression in human prostate cancer is directly suppressed by the androgen receptor. Oncogene. 2014;33:504-13 pubmed publisher
  36. Estarás C, Fueyo R, Akizu N, Beltran S, Martínez Balbás M. RNA polymerase II progression through H3K27me3-enriched gene bodies requires JMJD3 histone demethylase. Mol Biol Cell. 2013;24:351-60 pubmed publisher
  37. Zediak V, Johnnidis J, Wherry E, Berger S. Cutting edge: persistently open chromatin at effector gene loci in resting memory CD8+ T cells independent of transcriptional status. J Immunol. 2011;186:2705-9 pubmed publisher
  38. Rechtsteiner A, Ercan S, Takasaki T, Phippen T, Egelhofer T, Wang W, et al. The histone H3K36 methyltransferase MES-4 acts epigenetically to transmit the memory of germline gene expression to progeny. PLoS Genet. 2010;6:e1001091 pubmed publisher