This is a Validated Antibody Database (VAD) review about cow NPM1, based on 15 published articles (read how Labome selects the articles), using NPM1 antibody in all methods. It is aimed to help Labome visitors find the most suited NPM1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NPM1 synonym: nucleophosmin; NPM; nucleophosmin (nucleolar phosphoprotein B23, numatrin)

Abcam
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:100; loading ...; fig 1f
In order to show that macroH2A variants aid in maintaining nuclear organization and heterochromatin architecture, Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on human samples at 1:100 (fig 1f). J Cell Sci (2017) ncbi
mouse monoclonal (FC82291)
  • western blot; human; loading ...; fig 1a
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on human samples (fig 1a). Oncotarget (2016) ncbi
mouse monoclonal (FC82291)
  • western blot; human; fig s1
Abcam NPM1 antibody (Abcam, Ab10530) was used in western blot on human samples (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (FC82291)
  • immunohistochemistry - paraffin section; human; 1:5000; loading ...; fig 6a
  • immunocytochemistry; human; 1:500; loading ...; fig 1a
  • western blot; human; loading ...; fig 1c
Abcam NPM1 antibody (Abcam, ab10530) was used in immunohistochemistry - paraffin section on human samples at 1:5000 (fig 6a), in immunocytochemistry on human samples at 1:500 (fig 1a) and in western blot on human samples (fig 1c). Oncotarget (2016) ncbi
mouse monoclonal (FC82291)
  • western blot; mouse; fig 4
  • immunocytochemistry; human; 1:200; fig s2
  • western blot; human; fig 5
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on mouse samples (fig 4), in immunocytochemistry on human samples at 1:200 (fig s2) and in western blot on human samples (fig 5). BMC Cell Biol (2015) ncbi
mouse monoclonal (FC82291)
  • western blot; mouse
Abcam NPM1 antibody (Abcam, FC82291) was used in western blot on mouse samples . Breast Cancer Res (2015) ncbi
mouse monoclonal (FC82291)
  • other; human; fig 2
In order to characterize capabilities and potentials of extracellular vesicle (EV) array, Abcam NPM1 antibody (Abcam, ab10530) was used in other on human samples (fig 2). J Extracell Vesicles (2015) ncbi
mouse monoclonal (FC82291)
  • immunoprecipitation; human; loading ...; fig 1a
  • immunocytochemistry; human; loading ...; fig s1
  • western blot; human; loading ...; fig 6a
Abcam NPM1 antibody (Abcam, ab10530) was used in immunoprecipitation on human samples (fig 1a), in immunocytochemistry on human samples (fig s1) and in western blot on human samples (fig 6a). Sci Rep (2015) ncbi
mouse monoclonal (FC82291)
  • immunoprecipitation; human; fig 6c
  • western blot; human; 1:3000; fig 7g
Abcam NPM1 antibody (Abcam, ab10530) was used in immunoprecipitation on human samples (fig 6c) and in western blot on human samples at 1:3000 (fig 7g). Nat Commun (2015) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human
Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on human samples . J Neurochem (2014) ncbi
mouse monoclonal (FC82291)
  • western blot; human; fig 2
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on human samples (fig 2). Oncotarget (2014) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; mouse
Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on mouse samples . Stem Cells Dev (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (FC82291)
  • immunocytochemistry; mouse; 1:200; fig s1
Santa Cruz Biotechnology NPM1 antibody (Santa Cruz, SC56622) was used in immunocytochemistry on mouse samples at 1:200 (fig s1). EMBO J (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D19C1)
  • western blot; human; 1:1000; loading ...; fig 4d
Cell Signaling Technology NPM1 antibody (Cell Signalling Technology, 3520) was used in western blot on human samples at 1:1000 (fig 4d). EMBO Mol Med (2019) ncbi
rabbit monoclonal (D19C1)
  • western blot; human; loading ...; fig 3
In order to report that PPM1D modulates nucleolar formation by regulating NPM phosphorylation, Cell Signaling Technology NPM1 antibody (Cell Signaling, D19C1) was used in western blot on human samples (fig 3). Sci Rep (2016) ncbi
Articles Reviewed
  1. Singh R, Peng S, Viswanath P, Sambandam V, Shen L, Rao X, et al. Non-canonical cMet regulation by vimentin mediates Plk1 inhibitor-induced apoptosis. EMBO Mol Med. 2019;: pubmed publisher
  2. Douet J, Corujo D, Malinverni R, Renauld J, Sansoni V, Posavec Marjanović M, et al. MacroH2A histone variants maintain nuclear organization and heterochromatin architecture. J Cell Sci. 2017;130:1570-1582 pubmed publisher
  3. Huang M, Garcia J, Thomas D, Zhu L, Nguyen L, Chan S, et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. Oncotarget. 2016;7:74917-74930 pubmed publisher
  4. Kozakai Y, Kamada R, Furuta J, Kiyota Y, Chuman Y, Sakaguchi K. PPM1D controls nucleolar formation by up-regulating phosphorylation of nucleophosmin. Sci Rep. 2016;6:33272 pubmed publisher
  5. Vickers T, Crooke S. Development of a Quantitative BRET Affinity Assay for Nucleic Acid-Protein Interactions. PLoS ONE. 2016;11:e0161930 pubmed publisher
  6. Marzahn M, Marada S, Lee J, Nourse A, Kenrick S, Zhao H, et al. Higher-order oligomerization promotes localization of SPOP to liquid nuclear speckles. EMBO J. 2016;35:1254-75 pubmed publisher
  7. Destouches D, Sader M, Terry S, Marchand C, Maillé P, Soyeux P, et al. Implication of NPM1 phosphorylation and preclinical evaluation of the nucleoprotein antagonist N6L in prostate cancer. Oncotarget. 2016;7:69397-69411 pubmed publisher
  8. Ponti D, Bastianelli D, Rosa P, Pacini L, Ibrahim M, Rendina E, et al. The expression of B23 and EGR1 proteins is functionally linked in tumor cells under stress conditions. BMC Cell Biol. 2015;16:27 pubmed publisher
  9. Tancioni I, Miller N, Uryu S, Lawson C, Jean C, Chen X, et al. FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth. Breast Cancer Res. 2015;17:47 pubmed publisher
  10. Jørgensen M, Bæk R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048 pubmed publisher
  11. Arnoldo L, Sgarra R, Chiefari E, Iiritano S, Arcidiacono B, Pegoraro S, et al. A novel mechanism of post-translational modulation of HMGA functions by the histone chaperone nucleophosmin. Sci Rep. 2015;5:8552 pubmed publisher
  12. Wang L, Liu R, Ye P, Wong C, Chen G, Zhou P, et al. Intracellular CD24 disrupts the ARF-NPM interaction and enables mutational and viral oncogene-mediated p53 inactivation. Nat Commun. 2015;6:5909 pubmed publisher
  13. Khalouei S, Chow A, Brown I. Localization of heat shock protein HSPA6 (HSP70B') to sites of transcription in cultured differentiated human neuronal cells following thermal stress. J Neurochem. 2014;131:743-54 pubmed publisher
  14. Hamilton G, Abraham A, Morton J, Sampson O, Pefani D, Khoronenkova S, et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget. 2014;5:6142-67 pubmed
  15. Lührig S, Siamishi I, Tesmer Wolf M, Zechner U, Engel W, Nolte J. Lrrc34, a novel nucleolar protein, interacts with npm1 and ncl and has an impact on pluripotent stem cells. Stem Cells Dev. 2014;23:2862-74 pubmed publisher