This is a Validated Antibody Database (VAD) review about cow NOTCH1, based on 17 published articles (read how Labome selects the articles), using NOTCH1 antibody in all methods. It is aimed to help Labome visitors find the most suited NOTCH1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NOTCH1 synonym: neurogenic locus notch homolog protein 1; Notch gene homolog 1; Notch homolog 1, translocation-associated

Knockout validation
Abcam
rabbit monoclonal (EP1238Y)
  • immunohistochemistry knockout validation; mouse; fig 4
  • immunohistochemistry - paraffin section; mouse; fig 4
In order to assess the effect of Notch1 inactivation in oroesophageal epithelium, Abcam NOTCH1 antibody (Abcam, EP1238Y) was used in immunohistochemistry knockout validation on mouse samples (fig 4) and in immunohistochemistry - paraffin section on mouse samples (fig 4). Biochem Biophys Res Commun (2016) ncbi
Abcam
rabbit monoclonal (EP1238Y)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 3a
Abcam NOTCH1 antibody (Abcam, ab52627) was used in immunohistochemistry knockout validation on mouse samples (fig 3a). PLoS ONE (2015) ncbi
Abcam
rabbit monoclonal (EP1238Y)
  • western blot; mouse; 1:1000; loading ...; fig 5o
Abcam NOTCH1 antibody (Abcam, ab52627) was used in western blot on mouse samples at 1:1000 (fig 5o). Science (2018) ncbi
rabbit monoclonal (EP1238Y)
  • western blot; mouse; 1:200; loading ...; fig 3c
Abcam NOTCH1 antibody (Abcam, EP1238Y) was used in western blot on mouse samples at 1:200 (fig 3c). J Clin Invest (2017) ncbi
rabbit monoclonal (EP1238Y)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 6b
  • immunocytochemistry; human; fig 8a
In order to use knockout mice to determine the role of a2V in mammary gland development, Abcam NOTCH1 antibody (Abcam, ab52627) was used in immunohistochemistry - frozen section on mouse samples (fig 6b) and in immunocytochemistry on human samples (fig 8a). Cell Death Dis (2016) ncbi
rabbit monoclonal (EP1238Y)
  • western blot; human; loading ...; fig s2
Abcam NOTCH1 antibody (Abcam, ab52627) was used in western blot on human samples (fig s2). Proc Natl Acad Sci U S A (2016) ncbi
rabbit monoclonal (EP1238Y)
  • western blot; mouse; fig 6
  • western blot; human; fig 4
In order to analyze mediation of pathological retinal neovascularization via modulating DLL4-NOTCH1 signaling due to the cyclic AMP response element binding protein, Abcam NOTCH1 antibody (Abcam, ab52627) was used in western blot on mouse samples (fig 6) and in western blot on human samples (fig 4). EBioMedicine (2015) ncbi
rabbit monoclonal (EP1238Y)
  • immunohistochemistry knockout validation; mouse; fig 4
  • immunohistochemistry - paraffin section; mouse; fig 4
In order to assess the effect of Notch1 inactivation in oroesophageal epithelium, Abcam NOTCH1 antibody (Abcam, EP1238Y) was used in immunohistochemistry knockout validation on mouse samples (fig 4) and in immunohistochemistry - paraffin section on mouse samples (fig 4). Biochem Biophys Res Commun (2016) ncbi
rabbit monoclonal (EP1238Y)
  • western blot; human; 1:1000; loading ...; fig 2b
Abcam NOTCH1 antibody (Abcam, ab52627) was used in western blot on human samples at 1:1000 (fig 2b). Mol Med Rep (2016) ncbi
rabbit monoclonal (EP1238Y)
  • immunohistochemistry - paraffin section; human; loading ...; fig 3a
Abcam NOTCH1 antibody (Abcam, ab52627) was used in immunohistochemistry - paraffin section on human samples (fig 3a). Oncotarget (2016) ncbi
rabbit monoclonal (EP1238Y)
  • immunohistochemistry knockout validation; mouse; loading ...; fig 3a
Abcam NOTCH1 antibody (Abcam, ab52627) was used in immunohistochemistry knockout validation on mouse samples (fig 3a). PLoS ONE (2015) ncbi
rabbit monoclonal (EP1238Y)
  • immunocytochemistry; human; 5.0 ug/ml
In order to investigate the role of Notch signaling in human basal cell differentiation, Abcam NOTCH1 antibody (Abcam, ab52627) was used in immunocytochemistry on human samples at 5.0 ug/ml. PLoS ONE (2015) ncbi
rabbit monoclonal (EP1238Y)
  • western blot; human; 1:5000
In order to examine Notch localization and activation in non-small cell lung cancer, Abcam NOTCH1 antibody (Abcam, EP1238Y) was used in western blot on human samples at 1:5000. J Hematol Oncol (2015) ncbi
rabbit monoclonal (EP1238Y)
  • western blot; human; loading ...; fig 6a
Abcam NOTCH1 antibody (Abcam, ab52627) was used in western blot on human samples (fig 6a). Int J Mol Med (2015) ncbi
rabbit monoclonal (EP1238Y)
  • western blot; mouse
Abcam NOTCH1 antibody (Abcam, ab52627) was used in western blot on mouse samples . PLoS ONE (2014) ncbi
rabbit monoclonal (EP1238Y)
  • immunohistochemistry - paraffin section; human; 1:200; loading ...; fig 3c
Abcam NOTCH1 antibody (Abcam, EP1238Y) was used in immunohistochemistry - paraffin section on human samples at 1:200 (fig 3c). J Neurooncol (2014) ncbi
rabbit monoclonal (EP1238Y)
  • immunohistochemistry - paraffin section; mouse; 1:50
Abcam NOTCH1 antibody (Abcam, Ab52627) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. PLoS ONE (2013) ncbi
Invitrogen
mouse monoclonal (mN1A)
  • immunoprecipitation; human
In order to elucidate how Notch signaling contributes to T cell acute lymphoblastic leukemia using mice, Invitrogen NOTCH1 antibody (eBioscience, mN1A) was used in immunoprecipitation on human samples . PLoS ONE (2013) ncbi
Cell Signaling Technology
rat monoclonal (5B5)
  • western blot; human
In order to examine the effects of kinase inhibtors that target the epidermal growth factor receptor in lung cancer, Cell Signaling Technology NOTCH1 antibody (Cell Signaling, 5B5) was used in western blot on human samples . Cancer Res (2014) ncbi
Articles Reviewed
  1. Chakrabarti R, Celià Terrassa T, Kumar S, Hang X, Wei Y, Choudhury A, et al. Notch ligand Dll1 mediates cross-talk between mammary stem cells and the macrophageal niche. Science. 2018;360: pubmed publisher
  2. Hirai M, Arita Y, McGlade C, Lee K, Chen J, Evans S. Adaptor proteins NUMB and NUMBL promote cell cycle withdrawal by targeting ERBB2 for degradation. J Clin Invest. 2017;127:569-582 pubmed publisher
  3. Pamarthy S, Mao L, Katara G, Fleetwood S, Kulshreshta A, Gilman Sachs A, et al. The V-ATPase a2 isoform controls mammary gland development through Notch and TGF-β signaling. Cell Death Dis. 2016;7:e2443 pubmed publisher
  4. Stoyanova T, Riedinger M, Lin S, Faltermeier C, Smith B, Zhang K, et al. Activation of Notch1 synergizes with multiple pathways in promoting castration-resistant prostate cancer. Proc Natl Acad Sci U S A. 2016;113:E6457-E6466 pubmed
  5. Singh N, Kotla S, Kumar R, Rao G. Cyclic AMP Response Element Binding Protein Mediates Pathological Retinal Neovascularization via Modulating DLL4-NOTCH1 Signaling. EBioMedicine. 2015;2:1767-84 pubmed publisher
  6. Mandasari M, Sawangarun W, Katsube K, Kayamori K, Yamaguchi A, Sakamoto K. A facile one-step strategy for the generation of conditional knockout mice to explore the role of Notch1 in oroesophageal tumorigenesis. Biochem Biophys Res Commun. 2016;469:761-7 pubmed publisher
  7. Chen X, Dong X, Gao H, Jiang Y, Jin Y, Chang Y, et al. Suppression of HSP27 increases the anti‑tumor effects of quercetin in human leukemia U937 cells. Mol Med Rep. 2016;13:689-96 pubmed publisher
  8. Denicolaï E, Tabouret E, Colin C, Metellus P, Nanni I, Boucard C, et al. Molecular heterogeneity of glioblastomas: does location matter?. Oncotarget. 2016;7:902-13 pubmed publisher
  9. Shao H, Kong R, Ferrari M, Radtke F, Capobianco A, Liu Z. Notch1 Pathway Activity Determines the Regulatory Role of Cancer-Associated Fibroblasts in Melanoma Growth and Invasion. PLoS ONE. 2015;10:e0142815 pubmed publisher
  10. Gomi K, Arbelaez V, Crystal R, Walters M. Activation of NOTCH1 or NOTCH3 signaling skews human airway basal cell differentiation toward a secretory pathway. PLoS ONE. 2015;10:e0116507 pubmed publisher
  11. Nguyen D, Rubinstein L, Takebe N, Miele L, Tomaszewski J, Ivy P, et al. Notch1 phenotype and clinical stage progression in non-small cell lung cancer. J Hematol Oncol. 2015;8:9 pubmed publisher
  12. Xie F, Cai W, Liu Y, Li Y, Du B, Feng L, et al. Vaccarin attenuates the human EA.hy926 endothelial cell oxidative stress injury through inhibition of Notch signaling. Int J Mol Med. 2015;35:135-42 pubmed publisher
  13. Arasada R, Amann J, Rahman M, Huppert S, Carbone D. EGFR blockade enriches for lung cancer stem-like cells through Notch3-dependent signaling. Cancer Res. 2014;74:5572-84 pubmed publisher
  14. Redmond E, Liu W, Hamm K, Hatch E, Cahill P, Morrow D. Perivascular delivery of Notch 1 siRNA inhibits injury-induced arterial remodeling. PLoS ONE. 2014;9:e84122 pubmed publisher
  15. Xiong H, Maraver A, Latkowski J, Henderson T, Schlessinger K, Ding Y, et al. Characterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells. PLoS ONE. 2013;8:e84841 pubmed publisher
  16. Gupta R, Sharma M, Suri V, Kakkar A, Singh M, Sarkar C. Study of chromosome 9q gain, Notch pathway regulators and Tenascin-C in ependymomas. J Neurooncol. 2014;116:267-74 pubmed publisher
  17. Murta D, Batista M, Silva E, Trindade A, Henrique D, Duarte A, et al. Dynamics of Notch pathway expression during mouse testis post-natal development and along the spermatogenic cycle. PLoS ONE. 2013;8:e72767 pubmed publisher