This is a Validated Antibody Database (VAD) review about cow NEFM, based on 20 published articles (read how Labome selects the articles), using NEFM antibody in all methods. It is aimed to help Labome visitors find the most suited NEFM antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NEFM synonym: NEF3; NF-M; neurofilament medium polypeptide; 160 kDa neurofilament protein; major neuronal intermediate filament subunit; neurofilament 3; neurofilament M subunit; neurofilament triplet M protein

Abcam
rabbit polyclonal
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 1
Abcam NEFM antibody (Abcam, ab9034) was used in immunohistochemistry on mouse samples at 1:1000 (fig 1). J Comp Neurol (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 7
Abcam NEFM antibody (Abcam, ab9034) was used in immunocytochemistry on human samples (fig 7). Stem Cell Reports (2016) ncbi
rabbit polyclonal
  • western blot; mouse; 1:5000; fig 1
Abcam NEFM antibody (Abcam, ab9034) was used in western blot on mouse samples at 1:5000 (fig 1). Sci Rep (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; chicken; 1:500
In order to study the maturation of neurogenic placodes, Abcam NEFM antibody (Abcam, ab9034) was used in immunohistochemistry on chicken samples at 1:500. J Anat (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse
Abcam NEFM antibody (Abcam, ab9034) was used in immunohistochemistry - frozen section on mouse samples . PLoS ONE (2013) ncbi
EnCor Biotechnology
mouse monoclonal
  • immunohistochemistry - paraffin section; mouse; 1:2000; loading ...
EnCor Biotechnology NEFM antibody (Encor Biotechnology, MCA-3H11) was used in immunohistochemistry - paraffin section on mouse samples at 1:2000. Int J Mol Sci (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:750
In order to investigate the effect of hematogenous macrophage depletion on fibrotic scar and axonal growth after spinal cord injury, EnCor Biotechnology NEFM antibody (EnCor, RPCA-NFM) was used in immunohistochemistry on mouse samples at 1:750. Neurobiol Dis (2015) ncbi
Novus Biologicals
chicken polyclonal
  • immunocytochemistry; mouse; 1:500; loading ...; fig 2a
Novus Biologicals NEFM antibody (Novus, NB300-222) was used in immunocytochemistry on mouse samples at 1:500 (fig 2a). Sci Rep (2017) ncbi
EMD Millipore
rabbit polyclonal
  • immunocytochemistry; rat; loading ...; fig 7
In order to generate an atoxic derivative of Botulism neurotoxin/C1 and assess whether it retains neuron-specific targeting without concomitant toxic host responses, EMD Millipore NEFM antibody (Millipore, ab1987) was used in immunocytochemistry on rat samples (fig 7). Sci Rep (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; fig 2c
  • western blot; mouse; loading ...; fig 5f
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry on mouse samples at 1:200 (fig 2c) and in western blot on mouse samples (fig 5f). Nat Commun (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig 2a
  • western blot; mouse; loading ...; fig 2d
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunocytochemistry on mouse samples (fig 2a) and in western blot on mouse samples (fig 2d). J Cell Biol (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000; fig s3
In order to learn about induction of selective motor neuron degeneration through toxic gain of function due to ALS-associated mutant FUS, EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig s3). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - frozen section on mouse samples . Science (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:200; loading ...; fig s1d
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry on human samples at 1:200 (fig s1d). Stem Cells (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 6
EMD Millipore NEFM antibody (EMD Millipore, AB1987) was used in western blot on mouse samples (fig 6). Nat Neurosci (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig s4
In order to elucidate the molecular mechanisms underlying paranodal junction assembly, EMD Millipore NEFM antibody (Millipore, AB1987) was used in western blot on mouse samples (fig s4). Nat Neurosci (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; fig 5
EMD Millipore NEFM antibody (Chemicon, AB1987) was used in immunohistochemistry on mouse samples (fig 5). Biol Open (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - paraffin section on mouse samples . J Neurosci (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; chicken
In order to localize and characterize cholesterol-rich membrane microdomains in the inner ear, EMD Millipore NEFM antibody (Chemicon, AB1987) was used in immunohistochemistry - frozen section on chicken samples . J Proteomics (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse
In order to study the role of neuregulin-1/glial growth factor in Schwann cell migration and its mechanism, EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - frozen section on mouse samples . Genes Cells (2014) ncbi
Articles Reviewed
  1. Getz A, Xu F, Visser F, Persson R, Syed N. Tumor suppressor menin is required for subunit-specific nAChR α5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons. Sci Rep. 2017;7:1768 pubmed publisher
  2. Vazquez Cintron E, Beske P, Tenezaca L, Tran B, Oyler J, Glotfelty E, et al. Engineering Botulinum Neurotoxin C1 as a Molecular Vehicle for Intra-Neuronal Drug Delivery. Sci Rep. 2017;7:42923 pubmed publisher
  3. Brügger V, Duman M, Bochud M, Münger E, Heller M, Ruff S, et al. Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Nat Commun. 2017;8:14272 pubmed publisher
  4. Maggio D, Singh A, Iorgulescu J, Bleicher D, Ghosh M, Lopez M, et al. Identifying the Long-Term Role of Inducible Nitric Oxide Synthase after Contusive Spinal Cord Injury Using a Transgenic Mouse Model. Int J Mol Sci. 2017;18: pubmed publisher
  5. Wakatsuki S, Tokunaga S, Shibata M, Araki T. GSK3B-mediated phosphorylation of MCL1 regulates axonal autophagy to promote Wallerian degeneration. J Cell Biol. 2017;216:477-493 pubmed publisher
  6. Wang Q, Ng L, Harris J, Feng D, Li Y, Royall J, et al. Organization of the connections between claustrum and cortex in the mouse. J Comp Neurol. 2017;525:1317-1346 pubmed publisher
  7. Kushwaha R, Jagadish N, Kustagi M, Mendiratta G, Seandel M, Soni R, et al. Mechanism and Role of SOX2 Repression in Seminoma: Relevance to Human Germline Specification. Stem Cell Reports. 2016;6:772-783 pubmed publisher
  8. Sharma A, Lyashchenko A, Lu L, Nasrabady S, Elmaleh M, Mendelsohn M, et al. ALS-associated mutant FUS induces selective motor neuron degeneration through toxic gain of function. Nat Commun. 2016;7:10465 pubmed publisher
  9. Amin N, Bai G, Klug J, Bonanomi D, Pankratz M, Gifford W, et al. Loss of motoneuron-specific microRNA-218 causes systemic neuromuscular failure. Science. 2015;350:1525-9 pubmed publisher
  10. Wang H, Hsieh P, Huang D, Chin P, Chou C, Tung C, et al. RBFOX3/NeuN is Required for Hippocampal Circuit Balance and Function. Sci Rep. 2015;5:17383 pubmed publisher
  11. Uchida H, Morita T, Niizuma K, Kushida Y, Kuroda Y, Wakao S, et al. Transplantation of Unique Subpopulation of Fibroblasts, Muse Cells, Ameliorates Experimental Stroke Possibly via Robust Neuronal Differentiation. Stem Cells. 2016;34:160-73 pubmed publisher
  12. Smith A, Fleenor S, Begbie J. Changes in gene expression and cell shape characterise stages of epibranchial placode-derived neuron maturation in the chick. J Anat. 2015;227:89-102 pubmed publisher
  13. Zhu Y, Soderblom C, Krishnan V, Ashbaugh J, Bethea J, Lee J. Hematogenous macrophage depletion reduces the fibrotic scar and increases axonal growth after spinal cord injury. Neurobiol Dis. 2015;74:114-25 pubmed publisher
  14. Ho T, Zollinger D, Chang K, Xu M, Cooper E, Stankewich M, et al. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier. Nat Neurosci. 2014;17:1664-72 pubmed publisher
  15. Chang K, Zollinger D, Susuki K, Sherman D, Makara M, Brophy P, et al. Glial ankyrins facilitate paranodal axoglial junction assembly. Nat Neurosci. 2014;17:1673-81 pubmed publisher
  16. Liu C, Lin C, Gao C, May Simera H, Swaroop A, Li T. Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a. Biol Open. 2014;3:861-70 pubmed publisher
  17. Bull S, Bin J, Beaumont E, Boutet A, Krimpenfort P, Sadikot A, et al. Progressive disorganization of paranodal junctions and compact myelin due to loss of DCC expression by oligodendrocytes. J Neurosci. 2014;34:9768-78 pubmed publisher
  18. Thomas P, Cheng A, Colby C, Liu L, Patel C, Josephs L, et al. Localization and proteomic characterization of cholesterol-rich membrane microdomains in the inner ear. J Proteomics. 2014;103:178-93 pubmed publisher
  19. Wakatsuki S, Araki T, Sehara Fujisawa A. Neuregulin-1/glial growth factor stimulates Schwann cell migration by inducing ?5 ?1 integrin-ErbB2-focal adhesion kinase complex formation. Genes Cells. 2014;19:66-77 pubmed publisher
  20. Stegeman S, Jolly L, Premarathne S, Gecz J, Richards L, Mackay Sim A, et al. Loss of Usp9x disrupts cortical architecture, hippocampal development and TGF?-mediated axonogenesis. PLoS ONE. 2013;8:e68287 pubmed publisher