This is a Validated Antibody Database (VAD) review about cow MFN2, based on 21 published articles (read how Labome selects the articles), using MFN2 antibody in all methods. It is aimed to help Labome visitors find the most suited MFN2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
MFN2 synonym: mitofusin-2

Knockout validation
Abcam
mouse monoclonal
  • western blot knockout validation; human; 1:500; fig s1b
In order to investigate PINK1-Mfn2-Parkin-mediated mitophagy in mouse hearts, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on human samples at 1:500 (fig s1b). Science (2015) ncbi
Abcam
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig s4
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig s4). J Cell Biol (2015) ncbi
Abcam
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Cell Metab (2015) ncbi
Abcam
mouse monoclonal
  • western blot; mouse; loading ...; fig 4a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig 4a). Cell Rep (2018) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig s1c
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig s1c). Cell Rep (2018) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 3c
Abcam MFN2 antibody (abcam, ab56889) was used in western blot on mouse samples (fig 3c). J Mol Histol (2018) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...; fig 2a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples at 1:1000 (fig 2a). Endocrinology (2018) ncbi
mouse monoclonal
  • immunohistochemistry; mouse; loading ...; fig 5e
  • western blot; mouse; loading ...; fig 2a
  • western blot; human; loading ...; fig 5c
Abcam MFN2 antibody (Abcam, 56889) was used in immunohistochemistry on mouse samples (fig 5e), in western blot on mouse samples (fig 2a) and in western blot on human samples (fig 5c). Autophagy (2017) ncbi
mouse monoclonal
  • western blot; human; 1:5000; loading ...; fig 2a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples at 1:5000 (fig 2a). Brain (2017) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 4b
In order to evaluate an approach for correcting mitochondrial fusion, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig 4b). Nature (2016) ncbi
mouse monoclonal
  • western blot; mouse; 1:2000; fig 6
In order to study affects of acute minimization of brown adipose tissue function by increased reliance on muscle based thermogenesis, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples at 1:2000 (fig 6). J Biol Chem (2016) ncbi
mouse monoclonal
  • western blot; mouse; fig s4
In order to study inhibition of mitochondrial fragmentation and attenuation of kainic acid-induced hippocampal cell death by a mitochondrial division inhibitor, Mdivi-1, Abcam MFN2 antibody (Abcam, 56889) was used in western blot on mouse samples (fig s4). BMC Neurosci (2016) ncbi
mouse monoclonal
  • western blot; human; fig s1
Abcam MFN2 antibody (Abcam, 56889) was used in western blot on human samples (fig s1). J Immunol (2016) ncbi
mouse monoclonal
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 4
Abcam MFN2 antibody (Abcam, ab56889) was used in immunocytochemistry on mouse samples (fig 2) and in western blot on mouse samples (fig 4). Nature (2016) ncbi
mouse monoclonal
  • western blot knockout validation; human; 1:500; fig s1b
In order to investigate PINK1-Mfn2-Parkin-mediated mitophagy in mouse hearts, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on human samples at 1:500 (fig s1b). Science (2015) ncbi
mouse monoclonal
  • flow cytometry; mouse; fig 1h
In order to develop the MitoTimer protein to study individual mitochondria, Abcam MFN2 antibody (Abcam, ab56889) was used in flow cytometry on mouse samples (fig 1h). J Mol Cell Cardiol (2016) ncbi
mouse monoclonal
  • other; mouse; 1:500; fig s4
Abcam MFN2 antibody (Abcam, ab56889) was used in other on mouse samples at 1:500 (fig s4). Nat Commun (2015) ncbi
mouse monoclonal
  • western blot; rat; fig 6d
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on rat samples (fig 6d). Cell Res (2015) ncbi
mouse monoclonal
  • western blot; human; fig s1
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples (fig s1). Nat Neurosci (2015) ncbi
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig s4
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig s4). J Cell Biol (2015) ncbi
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Cell Metab (2015) ncbi
mouse monoclonal
  • western blot; mouse; fig 3
In order to study how obesity alters mitochondria-associated ER membranes, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig 3). Nat Med (2014) ncbi
mouse monoclonal
  • western blot; human; 1:500
In order to report and characterize the interaction between BECN1 and PARK2, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples at 1:500. Autophagy (2014) ncbi
Sigma-Aldrich
rabbit polyclonal
  • western blot; mouse; 1:1000; fig 5b
Sigma-Aldrich MFN2 antibody (Sigma-Aldrich, AV42420) was used in western blot on mouse samples at 1:1000 (fig 5b). Nat Commun (2016) ncbi
Articles Reviewed
  1. D Eletto M, Rossin F, Occhigrossi L, Farrace M, Faccenda D, Desai R, et al. Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75. Cell Rep. 2018;25:3573-3581.e4 pubmed publisher
  2. Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
  3. Zhao H, Pan W, Chen L, Luo Y, Xu R. Nur77 promotes cerebral ischemia-reperfusion injury via activating INF2-mediated mitochondrial fragmentation. J Mol Histol. 2018;49:599-613 pubmed publisher
  4. Viana Huete V, Guillen C, García G, Fernandez S, García Aguilar A, Kahn C, et al. Male Brown Fat-Specific Double Knockout of IGFIR/IR: Atrophy, Mitochondrial Fission Failure, Impaired Thermogenesis, and Obesity. Endocrinology. 2018;159:323-340 pubmed publisher
  5. Wang Q, Wu S, Zhu H, Ding Y, Dai X, Ouyang C, et al. Deletion of PRKAA triggers mitochondrial fission by inhibiting the autophagy-dependent degradation of DNM1L. Autophagy. 2017;13:404-422 pubmed publisher
  6. Puschmann A, Fiesel F, Caulfield T, Hudec R, Ando M, Truban D, et al. Heterozygous PINK1 p.G411S increases risk of Parkinson's disease via a dominant-negative mechanism. Brain. 2017;140:98-117 pubmed publisher
  7. Franco A, Kitsis R, Fleischer J, Gavathiotis E, Kornfeld O, Gong G, et al. Correcting mitochondrial fusion by manipulating mitofusin conformations. Nature. 2016;540:74-79 pubmed publisher
  8. Bal N, Maurya S, Singh S, Wehrens X, Periasamy M. Increased Reliance on Muscle-based Thermogenesis upon Acute Minimization of Brown Adipose Tissue Function. J Biol Chem. 2016;291:17247-57 pubmed publisher
  9. Kim H, Lee J, Park K, Kim W, Roh G. A mitochondrial division inhibitor, Mdivi-1, inhibits mitochondrial fragmentation and attenuates kainic acid-induced hippocampal cell death. BMC Neurosci. 2016;17:33 pubmed publisher
  10. Kobayashi K, Araya J, Minagawa S, Hara H, Saito N, Kadota T, et al. Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis. J Immunol. 2016;197:504-16 pubmed publisher
  11. Prieto J, León M, Ponsoda X, Sendra R, Bort R, Ferrer Lorente R, et al. Early ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming. Nat Commun. 2016;7:11124 pubmed publisher
  12. Luchsinger L, de Almeida M, Corrigan D, Mumau M, Snoeck H. Mitofusin 2 maintains haematopoietic stem cells with extensive lymphoid potential. Nature. 2016;529:528-31 pubmed publisher
  13. Gong G, Song M, Csordás G, Kelly D, Matkovich S, Dorn G. Parkin-mediated mitophagy directs perinatal cardiac metabolic maturation in mice. Science. 2015;350:aad2459 pubmed publisher
  14. Stotland A, Gottlieb R. α-MHC MitoTimer mouse: In vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart. J Mol Cell Cardiol. 2016;90:53-8 pubmed publisher
  15. Wu S, Kao C, Wang L, Creighton C, Yang J, Donti T, et al. Increased COUP-TFII expression in adult hearts induces mitochondrial dysfunction resulting in heart failure. Nat Commun. 2015;6:8245 pubmed publisher
  16. Wang C, Du W, Su Q, Zhu M, Feng P, Li Y, et al. Dynamic tubulation of mitochondria drives mitochondrial network formation. Cell Res. 2015;25:1108-20 pubmed publisher
  17. Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, et al. Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci. 2015;18:501-10 pubmed publisher
  18. Mourier A, Motori E, Brandt T, Lagouge M, Atanassov I, Galinier A, et al. Mitofusin 2 is required to maintain mitochondrial coenzyme Q levels. J Cell Biol. 2015;208:429-42 pubmed publisher
  19. Song M, Mihara K, Chen Y, Scorrano L, Dorn G. Mitochondrial fission and fusion factors reciprocally orchestrate mitophagic culling in mouse hearts and cultured fibroblasts. Cell Metab. 2015;21:273-85 pubmed publisher
  20. Arruda A, Pers B, Parlakgül G, Güney E, Inouye K, Hotamisligil G. Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity. Nat Med. 2014;20:1427-35 pubmed publisher
  21. Choubey V, Cagalinec M, Liiv J, Safiulina D, Hickey M, Kuum M, et al. BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy. 2014;10:1105-19 pubmed publisher