This is a Validated Antibody Database (VAD) review about cow HSPD1, based on 30 published articles (read how Labome selects the articles), using HSPD1 antibody in all methods. It is aimed to help Labome visitors find the most suited HSPD1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HSPD1 synonym: HSP60; 60 kDa heat shock protein, mitochondrial; 60 kDa chaperonin; CPN60; HSP-60; chaperonin 60; heat shock 60kDa protein 1 (chaperonin); heat shock protein 60; mitochondrial matrix protein P1

Enzo Life Sciences
mouse monoclonal (LK-1)
  • western blot; mouse; loading ...; fig 1c
Enzo Life Sciences HSPD1 antibody (Enzo Life Sciences, ADI-SPA-806) was used in western blot on mouse samples (fig 1c). Cell Rep (2018) ncbi
mouse monoclonal (LK-1)
  • western blot; fruit fly; 1:1000; loading ...; fig 1b
Enzo Life Sciences HSPD1 antibody (Enzo Life Sciences, ADI-SPA 806-D) was used in western blot on fruit fly samples at 1:1000 (fig 1b). Am J Cancer Res (2017) ncbi
mouse monoclonal (LK-2)
  • immunocytochemistry; human; 1:5000; loading ...; fig 1b
  • immunocytochemistry; African green monkey; 1:5000; loading ...; fig 1d
  • immunocytochemistry; mouse; 1:5000; loading ...; fig 2b
In order to provide evidence for two ARL2-sensitive pathways in mitochondria, Enzo Life Sciences HSPD1 antibody (Enzo Life Sciences, ADI-SPA-807) was used in immunocytochemistry on human samples at 1:5000 (fig 1b), in immunocytochemistry on African green monkey samples at 1:5000 (fig 1d) and in immunocytochemistry on mouse samples at 1:5000 (fig 2b). PLoS ONE (2017) ncbi
mouse monoclonal (LK-2)
  • immunocytochemistry; human; 1:5000; loading ...; fig 2
In order to develop plasmids to study mitochondrial proteins, Enzo Life Sciences HSPD1 antibody (Enzo Life Sciences, ADI-SPA-807) was used in immunocytochemistry on human samples at 1:5000 (fig 2). Cell Logist (2016) ncbi
mouse monoclonal (LK-1)
  • western blot; mouse; loading ...; fig 3b
In order to use a mitochondrial single-channel patch clamp and cyclophilin D-deficient mice with streptozotocin-induced diabetes to study mitochondrial dysfunction, Enzo Life Sciences HSPD1 antibody (Enzo, ADISPA806D) was used in western blot on mouse samples (fig 3b). Diabetes (2016) ncbi
rabbit polyclonal
  • western blot; scFv; fig 7
In order to determine the affect of cellular bioenergetics in marine molluscs and functional reorganization of mitochondria by intermittent hypoxia, Enzo Life Sciences HSPD1 antibody (Stressgen Bioreagents, SPA-805) was used in western blot on scFv samples (fig 7). J Exp Biol (2016) ncbi
mouse monoclonal (LK-1)
  • western blot; mouse; 1:2000; fig 1
Enzo Life Sciences HSPD1 antibody (Enzo, ADI-SPA-806) was used in western blot on mouse samples at 1:2000 (fig 1). Redox Biol (2016) ncbi
mouse monoclonal (LK-2)
  • western blot; human; 1:4000; loading ...; fig 1
In order to study the effect of strength training on mitochondrial proteins and indicators of muscle cellular stress in patients with prostrate cancer receiving androgen deprivation therapy, Enzo Life Sciences HSPD1 antibody (Enzo Life, SPA-807-E) was used in western blot on human samples at 1:4000 (fig 1). Endocr Connect (2016) ncbi
mouse monoclonal (LK-1)
  • western blot; mouse; loading ...; fig 3a
Enzo Life Sciences HSPD1 antibody (Enzo Life Sciences, ADI-SPA-806) was used in western blot on mouse samples (fig 3a). J Gerontol A Biol Sci Med Sci (2017) ncbi
mouse monoclonal (Mab-11-13)
  • western blot; human; 1:5000; fig 1a
Enzo Life Sciences HSPD1 antibody (Stressgen Bioreagents, SPA-829) was used in western blot on human samples at 1:5000 (fig 1a). PLoS ONE (2016) ncbi
mouse monoclonal (Mab-11-13)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Enzo Life Sciences HSPD1 antibody (ENZO, Mab-11-13) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (LK-2)
  • western blot; Podospora anserina; 0.25 ug/ml; fig 2
Enzo Life Sciences HSPD1 antibody (Stressgen, SPA-807) was used in western blot on Podospora anserina samples at 0.25 ug/ml (fig 2). Sci Rep (2015) ncbi
mouse monoclonal (LK-2)
  • blocking or activating experiments; mouse; fig 3
Enzo Life Sciences HSPD1 antibody (Enzo Life Sciences, ADI-SPA-807E) was used in blocking or activating experiments on mouse samples (fig 3). Cell Stress Chaperones (2015) ncbi
mouse monoclonal (LK-1)
  • western blot; rat; 1:1200
Enzo Life Sciences HSPD1 antibody (Stressgen, SPA-806) was used in western blot on rat samples at 1:1200. Neurobiol Dis (2015) ncbi
mouse monoclonal (Mab-11-13)
  • western blot; human; fig 3
Enzo Life Sciences HSPD1 antibody (Enzo Life Sciences, SPA-829) was used in western blot on human samples (fig 3). J Biol Chem (2015) ncbi
mouse monoclonal (LK-1)
  • western blot; Garra rufa
In order to use Garra rufa to study heat shock proteins in different oxidative stress and thermal conditions, Enzo Life Sciences HSPD1 antibody (StressGen, SPA-806) was used in western blot on Garra rufa samples . Redox Biol (2014) ncbi
mouse monoclonal (LK-1)
  • western blot; rat; 1:2000
Enzo Life Sciences HSPD1 antibody (Stressgen, ADI-SPA 806) was used in western blot on rat samples at 1:2000. PLoS ONE (2014) ncbi
Abcam
mouse monoclonal (Mab11-13)
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 5d
  • western blot; mouse; 1:1000; loading ...; fig 5f
In order to characterize an apoptotic marker heat shock protein 60-based apoptosis imaging probe, Abcam HSPD1 antibody (Abcam, ab13532) was used in immunocytochemistry on mouse samples at 1:1000 (fig 5d) and in western blot on mouse samples at 1:1000 (fig 5f). Oncogenesis (2016) ncbi
mouse monoclonal (Mab11-13)
  • western blot; mouse; 1:1000; fig 1
Abcam HSPD1 antibody (Abcam, ab13532) was used in western blot on mouse samples at 1:1000 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (Mab11-13)
  • western blot; human; fig 2
In order to elucidate the role of EMRE as a matrix Ca(2+) sensor that controls gatekeeping of the mitochondrial Ca(2+) uniporter, Abcam HSPD1 antibody (abcam, ab13532) was used in western blot on human samples (fig 2). Cell Rep (2016) ncbi
mouse monoclonal (Mab11-13)
  • immunohistochemistry - paraffin section; human; 1:100; fig 4
In order to identify genes important for human spermatogenesis, Abcam HSPD1 antibody (Abcam, ab13532) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 4). Sci Rep (2016) ncbi
rabbit polyclonal
  • western blot; cow; 1:8000
In order to determine the role of endogenous IGFBP-3 in anisomycin-induced apoptosis, Abcam HSPD1 antibody (Abcam, ab31115) was used in western blot on cow samples at 1:8000. Endocrinology (2015) ncbi
Santa Cruz Biotechnology
mouse monoclonal (LK1)
  • western blot; human; loading ...; fig 6a
Santa Cruz Biotechnology HSPD1 antibody (Santa Cruz, sc-59567) was used in western blot on human samples (fig 6a). Cell Death Differ (2018) ncbi
mouse monoclonal (SPM253)
  • western blot; human; fig 5
Santa Cruz Biotechnology HSPD1 antibody (Santa Cruz, sc-65568) was used in western blot on human samples (fig 5). Cell Death Dis (2015) ncbi
Cell Signaling Technology
rabbit monoclonal (D6F1)
  • immunocytochemistry; African green monkey; 1:2000; loading ...; fig 3b
  • western blot; human; 1:2500; loading ...; fig 4a
In order to research the role of mutations of the aminoacyl-tRNA-synthetases SARS and WARS2 in the pathogenesis of autosomal recessive intellectual disability, Cell Signaling Technology HSPD1 antibody (Cell Signaling, 12165) was used in immunocytochemistry on African green monkey samples at 1:2000 (fig 3b) and in western blot on human samples at 1:2500 (fig 4a). Hum Mutat (2017) ncbi
rabbit monoclonal (D6F1)
  • western blot; human; loading ...; fig 2g
Cell Signaling Technology HSPD1 antibody (Cell Signaling, 12165) was used in western blot on human samples (fig 2g). Oncotarget (2017) ncbi
rabbit monoclonal (D6F1)
  • western blot; human; fig 7
In order to describe the effects of long- and short-term exposure to erythropoietin on white adipose tissue, Cell Signaling Technology HSPD1 antibody (Cell signaling, 12165) was used in western blot on human samples (fig 7). Lipids Health Dis (2016) ncbi
rabbit monoclonal (D6F1)
  • western blot; human; loading ...; fig 3a
Cell Signaling Technology HSPD1 antibody (cell signalling, 12165) was used in western blot on human samples (fig 3a). Oncotarget (2016) ncbi
rabbit monoclonal (D6F1)
  • western blot; human; 1:1000; fig 4
Cell Signaling Technology HSPD1 antibody (Cell Signaling Tech, 12165S) was used in western blot on human samples at 1:1000 (fig 4). Sci Rep (2015) ncbi
rabbit monoclonal (D6F1)
  • flow cytometry; mouse; 1:100
Cell Signaling Technology HSPD1 antibody (Cell Signaling, 12165) was used in flow cytometry on mouse samples at 1:100. J Cell Physiol (2015) ncbi
Articles Reviewed
  1. D Eletto M, Rossin F, Occhigrossi L, Farrace M, Faccenda D, Desai R, et al. Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75. Cell Rep. 2018;25:3573-3581.e4 pubmed publisher
  2. Goiran T, Duplan E, Rouland L, El Manaa W, Lauritzen I, Dunys J, et al. Nuclear p53-mediated repression of autophagy involves PINK1 transcriptional down-regulation. Cell Death Differ. 2018;25:873-884 pubmed publisher
  3. Umegawachi T, Yoshida H, Koshida H, Yamada M, Ohkawa Y, Sato T, et al. Control of tissue size and development by a regulatory element in the yorkie 3'UTR. Am J Cancer Res. 2017;7:673-687 pubmed
  4. Newman L, Schiavon C, Zhou C, Kahn R. The abundance of the ARL2 GTPase and its GAP, ELMOD2, at mitochondria are modulated by the fusogenic activity of mitofusins and stressors. PLoS ONE. 2017;12:e0175164 pubmed publisher
  5. Musante L, Püttmann L, Kahrizi K, Garshasbi M, Hu H, Stehr H, et al. Mutations of the aminoacyl-tRNA-synthetases SARS and WARS2 are implicated in the etiology of autosomal recessive intellectual disability. Hum Mutat. 2017;38:621-636 pubmed publisher
  6. Li G, Fu R, Shen H, Zhou J, Hu X, Liu Y, et al. Polyphyllin I induces mitophagic and apoptotic cell death in human breast cancer cells by increasing mitochondrial PINK1 levels. Oncotarget. 2017;8:10359-10374 pubmed publisher
  7. Newman L, Schiavon C, Kahn R. Plasmids for variable expression of proteins targeted to the mitochondrial matrix or intermembrane space. Cell Logist. 2016;6:e1247939 pubmed publisher
  8. Christensen B, Nellemann B, Jørgensen J, Pedersen S, Jessen N. Erythropoietin does not activate erythropoietin receptor signaling or lipolytic pathways in human subcutaneous white adipose tissue in vivo. Lipids Health Dis. 2016;15:160 pubmed publisher
  9. Lee J, Lee W, Seol M, Lee S, Kim D, Kim H, et al. Coupling of LETM1 up-regulation with oxidative phosphorylation and platelet-derived growth factor receptor signaling via YAP1 transactivation. Oncotarget. 2016;7:66728-66739 pubmed publisher
  10. Yan S, Du F, Wu L, Zhang Z, Zhong C, Yu Q, et al. F1F0 ATP Synthase-Cyclophilin D Interaction Contributes to Diabetes-Induced Synaptic Dysfunction and Cognitive Decline. Diabetes. 2016;65:3482-3494 pubmed
  11. Ivanina A, Nesmelova I, Leamy L, Sokolov E, Sokolova I. Intermittent hypoxia leads to functional reorganization of mitochondria and affects cellular bioenergetics in marine molluscs. J Exp Biol. 2016;219:1659-74 pubmed publisher
  12. Pharaoh G, Pulliam D, Hill S, Sataranatarajan K, Van Remmen H. Ablation of the mitochondrial complex IV assembly protein Surf1 leads to increased expression of the UPR(MT) and increased resistance to oxidative stress in primary cultures of fibroblasts. Redox Biol. 2016;8:430-8 pubmed publisher
  13. Nilsen T, Thorsen L, Kirkegaard C, Ugelstad I, Fossa S, Raastad T. The effect of strength training on muscle cellular stress in prostate cancer patients on ADT. Endocr Connect. 2016;5:74-82 pubmed publisher
  14. Tamura Y, Matsunaga Y, Kitaoka Y, Hatta H. Effects of Heat Stress Treatment on Age-dependent Unfolded Protein Response in Different Types of Skeletal Muscle. J Gerontol A Biol Sci Med Sci. 2017;72:299-308 pubmed publisher
  15. Martínez Pizarro A, Desviat L, Ugarte M, Perez B, Richard E. Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects. PLoS ONE. 2016;11:e0150357 pubmed publisher
  16. Yang S, Meng J, Yang Y, Liu H, Liu J, Zhang Y, et al. A HSP60-targeting peptide for cell apoptosis imaging. Oncogenesis. 2016;5:e201 pubmed publisher
  17. Barone R, Macaluso F, Sangiorgi C, Campanella C, Marino Gammazza A, Moresi V, et al. Skeletal muscle Heat shock protein 60 increases after endurance training and induces peroxisome proliferator-activated receptor gamma coactivator 1 α1 expression. Sci Rep. 2016;6:19781 pubmed publisher
  18. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  19. Vais H, Mallilankaraman K, Mak D, Hoff H, Payne R, Tanis J, et al. EMRE Is a Matrix Ca(2+) Sensor that Governs Gatekeeping of the Mitochondrial Ca(2+) Uniporter. Cell Rep. 2016;14:403-410 pubmed publisher
  20. Zhu Z, Li C, Yang S, Tian R, Wang J, Yuan Q, et al. Dynamics of the Transcriptome during Human Spermatogenesis: Predicting the Potential Key Genes Regulating Male Gametes Generation. Sci Rep. 2016;6:19069 pubmed publisher
  21. Agostini Dreyer A, Jetzt A, Stires H, Cohick W. Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export. Endocrinology. 2015;156:4141-51 pubmed publisher
  22. Fischer F, Filippis C, Osiewacz H. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep. 2015;5:12697 pubmed publisher
  23. Sargsyan A, Cai J, Fandino L, Labasky M, Forostyan T, Colosimo L, et al. Rapid parallel measurements of macroautophagy and mitophagy in mammalian cells using a single fluorescent biosensor. Sci Rep. 2015;5:12397 pubmed publisher
  24. Heiserman J, Chen L, Kim B, Kim S, Tran A, Siebenborn N, et al. TLR4 mutation and HSP60-induced cell death in adult mouse cardiac myocytes. Cell Stress Chaperones. 2015;20:527-35 pubmed publisher
  25. Guan J, Zhang X, Sun W, Qi L, Wu J, Qin Z. DRAM1 regulates apoptosis through increasing protein levels and lysosomal localization of BAX. Cell Death Dis. 2015;6:e1624 pubmed publisher
  26. Van Laar V, Roy N, Liu A, Rajprohat S, Arnold B, Dukes A, et al. Glutamate excitotoxicity in neurons triggers mitochondrial and endoplasmic reticulum accumulation of Parkin, and, in the presence of N-acetyl cysteine, mitophagy. Neurobiol Dis. 2015;74:180-93 pubmed publisher
  27. Davis A, Qiao S, Lesson J, Rojo de la Vega M, Park S, Seanez C, et al. The quinone methide aurin is a heat shock response inducer that causes proteotoxic stress and Noxa-dependent apoptosis in malignant melanoma cells. J Biol Chem. 2015;290:1623-38 pubmed publisher
  28. Oksala N, Ekmekçi F, Ozsoy E, Kirankaya S, Kokkola T, Emecen G, et al. Natural thermal adaptation increases heat shock protein levels and decreases oxidative stress. Redox Biol. 2014;3:25-8 pubmed publisher
  29. Yang H, La T, Gurenko Z, Steenhuis P, Liu W, Isseroff R. Recovery and Cultivation of Keratinocytes From Shipped Mouse Skin. J Cell Physiol. 2015;230:242-5 pubmed publisher
  30. Moura C, Lollo P, Morato P, Nisishima L, Carneiro E, Amaya Farfan J. Whey protein hydrolysate enhances HSP90 but does not alter HSP60 and HSP25 in skeletal muscle of rats. PLoS ONE. 2014;9:e83437 pubmed publisher