This is a Validated Antibody Database (VAD) review about cow HSP90B1, based on 24 published articles (read how Labome selects the articles), using HSP90B1 antibody in all methods. It is aimed to help Labome visitors find the most suited HSP90B1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HSP90B1 synonym: TRA1; endoplasmin; 94 kDa glucose-regulated protein; GRP-94; GRP94; heat shock protein 90 kDa beta member 1; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta, member 1; tumor rejection antigen (gp96) 1

Knockout validation
Enzo Life Sciences
rat monoclonal (9G10)
  • immunohistochemistry knockout validation; mouse; fig 2
Enzo Life Sciences HSP90B1 antibody (Stressgen, 9G10) was used in immunohistochemistry knockout validation on mouse samples (fig 2). J Hepatol (2015) ncbi
Enzo Life Sciences
rat monoclonal (9G10)
  • western blot; mouse; loading ...; fig 3c
Enzo Life Sciences HSP90B1 antibody (Enzo Life Science, ADI-SPA-850) was used in western blot on mouse samples (fig 3c). Biochem Biophys Res Commun (2019) ncbi
rat monoclonal (9G10)
  • western blot; human; 1:1000; loading ...; fig 5a
Enzo Life Sciences HSP90B1 antibody (Enzo, ADI-SPA-850) was used in western blot on human samples at 1:1000 (fig 5a). Front Immunol (2018) ncbi
rat monoclonal (9G10)
  • ELISA; mouse; loading ...; fig s4c
Enzo Life Sciences HSP90B1 antibody (Enzo, ADI-SPA-850) was used in ELISA on mouse samples (fig s4c). Nat Cell Biol (2019) ncbi
rat monoclonal (9G10)
  • western blot; human; 1:1000; loading ...; fig 2b
Enzo Life Sciences HSP90B1 antibody (Enzo, ADI-SPA-850) was used in western blot on human samples at 1:1000 (fig 2b). J Exp Med (2018) ncbi
rat monoclonal (9G10)
  • western blot; mouse; loading ...; fig 1a
In order to investigate the involvement of Sec22b in antigen cross-presentation and antitumor immunity, Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, spa850) was used in western blot on mouse samples (fig 1a). J Exp Med (2017) ncbi
rat monoclonal (9G10)
  • immunohistochemistry; mouse; fig 2
Enzo Life Sciences HSP90B1 antibody (Enzo, SPA-850) was used in immunohistochemistry on mouse samples (fig 2). Sci Rep (2016) ncbi
rat monoclonal (9G10)
  • western blot; human; fig 1
Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, ADI-SPA-850-F) was used in western blot on human samples (fig 1). J Biol Chem (2016) ncbi
rat monoclonal (9G10)
  • western blot; human; fig s3
Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, 9G10) was used in western blot on human samples (fig s3). Proc Natl Acad Sci U S A (2016) ncbi
rabbit polyclonal
  • western blot; mouse; fig 3b
In order to test if 17-allylamino-demethoxygeldanamycin improves intestinal barrier function using two mouse graft versus host disease models, Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, ADI-SPA-851) was used in western blot on mouse samples (fig 3b). Oncogene (2016) ncbi
rat monoclonal (9G10)
  • western blot; human; fig 2
Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, 9G10) was used in western blot on human samples (fig 2). J Hematol Oncol (2015) ncbi
rat monoclonal (9G10)
  • western blot; human; 1:1000; fig 2
Enzo Life Sciences HSP90B1 antibody (Enzo, ADI-SPA-850) was used in western blot on human samples at 1:1000 (fig 2). Am J Physiol Gastrointest Liver Physiol (2015) ncbi
rat monoclonal (9G10)
  • flow cytometry; mouse; fig 7
In order to report that GP96 is required for T regulatory cell maintenance and function, Enzo Life Sciences HSP90B1 antibody (Stressgen, 9G10) was used in flow cytometry on mouse samples (fig 7). J Clin Invest (2015) ncbi
rat monoclonal (9G10)
  • immunohistochemistry knockout validation; mouse; fig 2
Enzo Life Sciences HSP90B1 antibody (Stressgen, 9G10) was used in immunohistochemistry knockout validation on mouse samples (fig 2). J Hepatol (2015) ncbi
rat monoclonal (9G10)
  • western blot; mouse; 1:15000
In order to study the regulation of autophagosome biogenesis by connexins, Enzo Life Sciences HSP90B1 antibody (Assay Designs, SPA-850) was used in western blot on mouse samples at 1:15000. Nat Cell Biol (2014) ncbi
rat monoclonal (9G10)
  • western blot; mouse
Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, 9G10) was used in western blot on mouse samples . J Biol Chem (2013) ncbi
rat monoclonal (9G10)
  • western blot; mouse
In order to study the role of local IGF production in skeletal muscle and body growth using GRP94 knockout mice, Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, 9G10) was used in western blot on mouse samples . FASEB J (2012) ncbi
rat monoclonal (9G10)
  • flow cytometry; mouse
Enzo Life Sciences HSP90B1 antibody (Enzo Life Sciences, 9G10) was used in flow cytometry on mouse samples . J Biol Chem (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (AC88)
  • western blot; mouse; fig 1
Santa Cruz Biotechnology HSP90B1 antibody (santa Cruz, sc-59577) was used in western blot on mouse samples (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (AC88)
  • western blot; human; fig 7
Santa Cruz Biotechnology HSP90B1 antibody (Santa Cruz Biotechnology, sc-59577) was used in western blot on human samples (fig 7). Oncogene (2016) ncbi
mouse monoclonal (AC88)
  • flow cytometry; human; fig 1e
In order to characterize immunogenic PEL cell death, revertion of PEL-induced immune suppression, and stimulation of DCs all triggered by capsaicin, Santa Cruz Biotechnology HSP90B1 antibody (Santa Cruz Biotechnology, sc-59577) was used in flow cytometry on human samples (fig 1e). Oncotarget (2015) ncbi
mouse monoclonal (S88)
  • western blot; mouse; fig 1
Santa Cruz Biotechnology HSP90B1 antibody (Santa Cruz, sc-59578) was used in western blot on mouse samples (fig 1). J Virol (2015) ncbi
mouse monoclonal (AC88)
  • western blot; human; fig 3
Santa Cruz Biotechnology HSP90B1 antibody (Santa Cruz Biotechnology, sc-59577) was used in western blot on human samples (fig 3). Nucleic Acids Res (2014) ncbi
mouse monoclonal (S88)
  • western blot; rat
In order to test if alpha-synuclein modulates neurotransmitter release, Santa Cruz Biotechnology HSP90B1 antibody (Santa Cruz Biotechnology, sc-59578) was used in western blot on rat samples . J Neurosci (2014) ncbi
Novus Biologicals
rat monoclonal (9G10)
  • immunohistochemistry - paraffin section; mouse; 1:100; loading ...; fig 1b
Novus Biologicals HSP90B1 antibody (Novus Biologicals, NB300-619) was used in immunohistochemistry - paraffin section on mouse samples at 1:100 (fig 1b). Mol Metab (2018) ncbi
Articles Reviewed
  1. Fu Y, Zhang B, Mu T. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochem Biophys Res Commun. 2019;511:356-362 pubmed publisher
  2. Gómez Fernández P, Urtasun A, Paton A, Paton J, Borrego F, Dersh D, et al. Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response. Front Immunol. 2018;9:2934 pubmed publisher
  3. Keklikoglou I, Cianciaruso C, Güç E, Squadrito M, Spring L, Tazzyman S, et al. Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. Nat Cell Biol. 2019;21:190-202 pubmed publisher
  4. Carpier J, Zucchetti A, Bataille L, Dogniaux S, Shafaq Zadah M, Bardin S, et al. Rab6-dependent retrograde traffic of LAT controls immune synapse formation and T cell activation. J Exp Med. 2018;215:1245-1265 pubmed publisher
  5. Vierra N, Dickerson M, Jordan K, Dadi P, Katdare K, Altman M, et al. TALK-1 reduces delta-cell endoplasmic reticulum and cytoplasmic calcium levels limiting somatostatin secretion. Mol Metab. 2018;9:84-97 pubmed publisher
  6. Alloatti A, Rookhuizen D, Joannas L, Carpier J, Iborra S, Magalhaes J, et al. Critical role for Sec22b-dependent antigen cross-presentation in antitumor immunity. J Exp Med. 2017;214:2231-2241 pubmed publisher
  7. Yamanaka T, Tosaki A, Miyazaki H, Kurosawa M, Koike M, Uchiyama Y, et al. Differential roles of NF-Y transcription factor in ER chaperone expression and neuronal maintenance in the CNS. Sci Rep. 2016;6:34575 pubmed publisher
  8. Pourcet B, Gage M, León T, Waddington K, Pello O, Steffensen K, et al. The nuclear receptor LXR modulates interleukin-18 levels in macrophages through multiple mechanisms. Sci Rep. 2016;6:25481 pubmed publisher
  9. Di X, Wang Y, Han D, Fu Y, Duerfeldt A, Blagg B, et al. Grp94 Protein Delivers γ-Aminobutyric Acid Type A (GABAA) Receptors to Hrd1 Protein-mediated Endoplasmic Reticulum-associated Degradation. J Biol Chem. 2016;291:9526-39 pubmed publisher
  10. Kowal J, Arras G, Colombo M, Jouve M, Morath J, Primdal Bengtson B, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016;113:E968-77 pubmed publisher
  11. Debruyne D, Bhatnagar N, Sharma B, Luther W, Moore N, Cheung N, et al. ALK inhibitor resistance in ALK(F1174L)-driven neuroblastoma is associated with AXL activation and induction of EMT. Oncogene. 2016;35:3681-91 pubmed publisher
  12. Joly A, Deepti A, Seignez A, Goloudina A, Hebrard S, Schmitt E, et al. The HSP90 inhibitor, 17AAG, protects the intestinal stem cell niche and inhibits graft versus host disease development. Oncogene. 2016;35:2842-51 pubmed publisher
  13. Granato M, Gilardini Montani M, Filardi M, Faggioni A, Cirone M. Capsaicin triggers immunogenic PEL cell death, stimulates DCs and reverts PEL-induced immune suppression. Oncotarget. 2015;6:29543-54 pubmed publisher
  14. Chhabra S, Jain S, Wallace C, Hong F, Liu B. High expression of endoplasmic reticulum chaperone grp94 is a novel molecular hallmark of malignant plasma cells in multiple myeloma. J Hematol Oncol. 2015;8:77 pubmed publisher
  15. Alkhateeb A, Buckett P, Gardeck A, Kim J, Byrne S, Fraenkel P, et al. The small molecule ferristatin II induces hepatic hepcidin expression in vivo and in vitro. Am J Physiol Gastrointest Liver Physiol. 2015;308:G1019-26 pubmed publisher
  16. Vashist S, Ureña L, Gonzalez Hernandez M, Choi J, de Rougemont A, Rocha Pereira J, et al. Molecular chaperone Hsp90 is a therapeutic target for noroviruses. J Virol. 2015;89:6352-63 pubmed publisher
  17. Zhang Y, Wu B, Metelli A, Thaxton J, Hong F, Rachidi S, et al. GP96 is a GARP chaperone and controls regulatory T cell functions. J Clin Invest. 2015;125:859-69 pubmed publisher
  18. Rachidi S, Sun S, Wu B, Jones E, Drake R, Ogretmen B, et al. Endoplasmic reticulum heat shock protein gp96 maintains liver homeostasis and promotes hepatocellular carcinogenesis. J Hepatol. 2015;62:879-88 pubmed publisher
  19. Maruyama A, Mimura J, Itoh K. Non-coding RNA derived from the region adjacent to the human HO-1 E2 enhancer selectively regulates HO-1 gene induction by modulating Pol II binding. Nucleic Acids Res. 2014;42:13599-614 pubmed publisher
  20. Ronzitti G, Bucci G, Emanuele M, Leo D, Sotnikova T, Mus L, et al. Exogenous ?-synuclein decreases raft partitioning of Cav2.2 channels inducing dopamine release. J Neurosci. 2014;34:10603-15 pubmed publisher
  21. Bejarano E, Yuste A, Patel B, Stout R, Spray D, Cuervo A. Connexins modulate autophagosome biogenesis. Nat Cell Biol. 2014;16:401-14 pubmed publisher
  22. Hong F, Liu B, Chiosis G, Gewirth D, Li Z. ?7 helix region of ?I domain is crucial for integrin binding to endoplasmic reticulum chaperone gp96: a potential therapeutic target for cancer metastasis. J Biol Chem. 2013;288:18243-8 pubmed publisher
  23. Barton E, Park S, James J, Makarewich C, Philippou A, Eletto D, et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J. 2012;26:3691-702 pubmed publisher
  24. Wu S, Hong F, Gewirth D, Guo B, Liu B, Li Z. The molecular chaperone gp96/GRP94 interacts with Toll-like receptors and integrins via its C-terminal hydrophobic domain. J Biol Chem. 2012;287:6735-42 pubmed publisher