This is a Validated Antibody Database (VAD) review about cow FN1, based on 5 published articles (read how Labome selects the articles), using FN1 antibody in all methods. It is aimed to help Labome visitors find the most suited FN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FN1 synonym: fibronectin; cumulus cell-specific fibronectin 1 transcript; embryo-specific fibronectin 1 transcript; fibronectin ED-A- region; fibronectin variable region

Invitrogen
mouse monoclonal (A32)
  • immunohistochemistry - frozen section; human; 1:100; loading ...; fig 3a
In order to study peroxynitrous acid-mediated fibronectin modifications and how they contribute to cellular dysfunction, Invitrogen FN1 antibody (Thermo, A32) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 3a). Free Radic Biol Med (2016) ncbi
mouse monoclonal (A32)
  • immunohistochemistry; human; 1:200
  • ELISA; human
In order to describe an antibody-based method for evaluating the conformation of the heparin 2 binding domain in fibronectin, and use it to determine the relative contributions of heparin and mechanical strain to the regulation of fibronectin conformation, Invitrogen FN1 antibody (Pierce, CSI 005-32-02) was used in immunohistochemistry on human samples at 1:200 and in ELISA on human samples . Matrix Biol (2014) ncbi
EMD Millipore
rabbit polyclonal
  • flow cytometry; human; 5 ug/ml; loading ...; fig 3
In order to evaluate the methodology of microsphere cytometry for studying cellular microenvironment, EMD Millipore FN1 antibody (Millipore, AB2047) was used in flow cytometry on human samples at 5 ug/ml (fig 3). Integr Biol (Camb) (2017) ncbi
rabbit polyclonal
  • western blot; human; fig 4
EMD Millipore FN1 antibody (Chemicon, AB2047) was used in western blot on human samples (fig 4). Int J Nanomedicine (2015) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human; 1:200
EMD Millipore FN1 antibody (Millipore, AB2047) was used in immunohistochemistry - paraffin section on human samples at 1:200. PLoS ONE (2013) ncbi
Articles Reviewed
  1. Ertsås H, Nolan G, Labarge M, Lorens J. Microsphere cytometry to interrogate microenvironment-dependent cell signaling. Integr Biol (Camb). 2017;9:123-134 pubmed publisher
  2. Degendorfer G, Chuang C, Kawasaki H, Hammer A, Malle E, Yamakura F, et al. Peroxynitrite-mediated oxidation of plasma fibronectin. Free Radic Biol Med. 2016;97:602-615 pubmed publisher
  3. Asiri A, Marwani H, Khan S, Webster T. Understanding greater cardiomyocyte functions on aligned compared to random carbon nanofibers in PLGA. Int J Nanomedicine. 2015;10:89-96 pubmed publisher
  4. Hubbard B, Buczek Thomas J, Nugent M, Smith M. Heparin-dependent regulation of fibronectin matrix conformation. Matrix Biol. 2014;34:124-31 pubmed publisher
  5. Franck D, Gil E, Adam R, Kaplan D, Chung Y, Estrada C, et al. Evaluation of silk biomaterials in combination with extracellular matrix coatings for bladder tissue engineering with primary and pluripotent cells. PLoS ONE. 2013;8:e56237 pubmed publisher