This is a Validated Antibody Database (VAD) review about cow FERMT2, based on 8 published articles (read how Labome selects the articles), using FERMT2 antibody in all methods. It is aimed to help Labome visitors find the most suited FERMT2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
FERMT2 synonym: fermitin family homolog 2

Knockout validation
EMD Millipore
mouse monoclonal (3A3)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • immunocytochemistry; mouse; 1:500; fig 3
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1) and in immunocytochemistry on mouse samples at 1:500 (fig 3). elife (2016) ncbi
EMD Millipore
mouse monoclonal (3A3)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 8B
  • immunocytochemistry; human; 1:200; loading ...; fig 5E
  • western blot; human; 1:1000; loading ...; fig 2A
In order to establish that Smurf1 controls Kindlin-2 protein levels in cells and hinders integrin activation, EMD Millipore FERMT2 antibody (EMD Millipore, Mab2617) was used in immunohistochemistry on mouse samples at 1:200 (fig 8B), in immunocytochemistry on human samples at 1:200 (fig 5E) and in western blot on human samples at 1:1000 (fig 2A). J Cell Biol (2017) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; 1:1000; loading ...; fig s1d
In order to measure the adhesion of alphaV-class and/or alpha5beta1 integrins expressing fibroblasts initiating attachment to fibronectin, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot on mouse samples at 1:1000 (fig s1d). Nat Commun (2017) ncbi
mouse monoclonal (3A3)
  • immunocytochemistry; human; 1:200; fig 3a
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunocytochemistry on human samples at 1:200 (fig 3a). Mol Med Rep (2016) ncbi
mouse monoclonal (3A3)
  • western blot knockout validation; mouse; 1:1000; fig 1
  • immunocytochemistry; mouse; 1:500; fig 3
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1) and in immunocytochemistry on mouse samples at 1:500 (fig 3). elife (2016) ncbi
mouse monoclonal (3A3)
  • immunocytochemistry; human; 1:400; fig 4
In order to describe a role for talin in sensing tissue stiffness, EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunocytochemistry on human samples at 1:400 (fig 4). Nat Cell Biol (2015) ncbi
mouse monoclonal (3A3)
  • immunoprecipitation; rat
  • immunocytochemistry; rat
  • western blot; rat
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in immunoprecipitation on rat samples , in immunocytochemistry on rat samples and in western blot on rat samples . FEBS Lett (2015) ncbi
mouse monoclonal (3A3)
  • western blot; mouse; fig 9
EMD Millipore FERMT2 antibody (Millipore, MAB2617) was used in western blot on mouse samples (fig 9). Mol Biol Cell (2015) ncbi
mouse monoclonal (3A3)
  • immunocytochemistry; human; fig 5a
EMD Millipore FERMT2 antibody (EMD Millipore, MAB2617) was used in immunocytochemistry on human samples (fig 5a). J Cell Biol (2015) ncbi
Articles Reviewed
  1. Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
  2. Bharadwaj M, Strohmeyer N, Colo G, Helenius J, Beerenwinkel N, Schiller H, et al. ?V-class integrins exert dual roles on ?5?1 integrins to strengthen adhesion to fibronectin. Nat Commun. 2017;8:14348 pubmed publisher
  3. Zeng F, Xie Y, Liao L, Li L, Chen B, Xie J, et al. Biological characterization of three immortalized esophageal epithelial cell lines. Mol Med Rep. 2016;14:4802-4810 pubmed publisher
  4. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  5. Austen K, Ringer P, Mehlich A, Chrostek Grashoff A, Kluger C, Klingner C, et al. Extracellular rigidity sensing by talin isoform-specific mechanical linkages. Nat Cell Biol. 2015;17:1597-606 pubmed publisher
  6. Qi L, Yu Y, Chi X, Xu W, Lu D, Song Y, et al. Kindlin-2 interacts with α-actinin-2 and β1 integrin to maintain the integrity of the Z-disc in cardiac muscles. FEBS Lett. 2015;589:2155-62 pubmed publisher
  7. Yazlovitskaya E, Tseng H, Viquez O, Tu T, Mernaugh G, McKee K, et al. Integrin α3β1 regulates kidney collecting duct development via TRAF6-dependent K63-linked polyubiquitination of Akt. Mol Biol Cell. 2015;26:1857-74 pubmed publisher
  8. Artym V, Swatkoski S, Matsumoto K, Campbell C, Petrie R, Dimitriadis E, et al. Dense fibrillar collagen is a potent inducer of invadopodia via a specific signaling network. J Cell Biol. 2015;208:331-50 pubmed publisher