This is a Validated Antibody Database (VAD) review about cow EPHA2, based on 8 published articles (read how Labome selects the articles), using EPHA2 antibody in all methods. It is aimed to help Labome visitors find the most suited EPHA2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EPHA2 synonym: ephrin type-A receptor 2

Knockout validation
Sigma-Aldrich
mouse monoclonal (D7)
  • western blot knockout validation; mouse; 1:500; fig 6
Sigma-Aldrich EPHA2 antibody (Sigma, E1026) was used in western blot knockout validation on mouse samples at 1:500 (fig 6). PLoS ONE (2012) ncbi
EMD Millipore
mouse monoclonal (D7)
  • immunoprecipitation; human; loading ...; fig 1b
  • immunocytochemistry; human; 1:200; loading ...; fig 3e
  • western blot; human; 1:1000; loading ...; fig 1b
In order to elucidate the mechanisms by which Rab-coupling protein contributes to metastasis, EMD Millipore EPHA2 antibody (Millipore, 05-480) was used in immunoprecipitation on human samples (fig 1b), in immunocytochemistry on human samples at 1:200 (fig 3e) and in western blot on human samples at 1:1000 (fig 1b). Nat Commun (2017) ncbi
mouse monoclonal (D7)
  • western blot; human; loading ...; fig 1c
EMD Millipore EPHA2 antibody (EMD Millipore, D7) was used in western blot on human samples (fig 1c). J Cell Biol (2016) ncbi
mouse monoclonal (D7)
  • immunoprecipitation; human; loading ...; fig 3f
  • western blot; human; loading ...; fig 3d
In order to screen for inhibitors of EphA2 canonical signaling, EMD Millipore EPHA2 antibody (Millipore, 05-480) was used in immunoprecipitation on human samples (fig 3f) and in western blot on human samples (fig 3d). Mol Biol Cell (2016) ncbi
mouse monoclonal (D7)
  • western blot; human; 1:1000; fig s4
In order to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers, EMD Millipore EPHA2 antibody (Millipore, 05-480) was used in western blot on human samples at 1:1000 (fig s4). Cancer Res (2016) ncbi
mouse monoclonal (D7)
  • western blot; human
In order to evaluate a genomic strategy for the evaluation of transforming growth factor-beta in breast cancer, EMD Millipore EPHA2 antibody (Millipore, 05-480) was used in western blot on human samples . Breast Cancer Res (2014) ncbi
mouse monoclonal (D7)
  • western blot; human; 1:1000
In order to study the effect of EPHA2 inhibition on cell apoptosis in non-small cell lung cancers, EMD Millipore EPHA2 antibody (Millipore, D7) was used in western blot on human samples at 1:1000. J Clin Invest (2014) ncbi
mouse monoclonal (D7)
  • immunoprecipitation; human
EMD Millipore EPHA2 antibody (Millipore, 05-480) was used in immunoprecipitation on human samples . PLoS ONE (2013) ncbi
Sigma-Aldrich
mouse monoclonal (D7)
  • western blot knockout validation; mouse; 1:500; fig 6
Sigma-Aldrich EPHA2 antibody (Sigma, E1026) was used in western blot knockout validation on mouse samples at 1:500 (fig 6). PLoS ONE (2012) ncbi
Articles Reviewed
  1. Gundry C, Marco S, Rainero E, Miller B, Dornier E, Mitchell L, et al. Phosphorylation of Rab-coupling protein by LMTK3 controls Rab14-dependent EphA2 trafficking to promote cell:cell repulsion. Nat Commun. 2017;8:14646 pubmed publisher
  2. Neill T, Buraschi S, Goyal A, Sharpe C, Natkanski E, Schaefer L, et al. EphA2 is a functional receptor for the growth factor progranulin. J Cell Biol. 2016;215:687-703 pubmed
  3. Barquilla A, Lamberto I, Noberini R, Heynen Genel S, Brill L, Pasquale E. Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell. 2016;27:2757-70 pubmed publisher
  4. Amato K, Wang S, Tan L, Hastings A, Song W, Lovly C, et al. EPHA2 Blockade Overcomes Acquired Resistance to EGFR Kinase Inhibitors in Lung Cancer. Cancer Res. 2016;76:305-18 pubmed publisher
  5. Sato M, Kadota M, Tang B, Yang H, Yang Y, Shan M, et al. An integrated genomic approach identifies persistent tumor suppressive effects of transforming growth factor-? in human breast cancer. Breast Cancer Res. 2014;16:R57 pubmed publisher
  6. Amato K, Wang S, Hastings A, Youngblood V, Santapuram P, Chen H, et al. Genetic and pharmacologic inhibition of EPHA2 promotes apoptosis in NSCLC. J Clin Invest. 2014;124:2037-49 pubmed publisher
  7. Sáinz Jaspeado M, Huertas Martínez J, Lagares Tena L, Martín Liberal J, Mateo Lozano S, de Alava E, et al. EphA2-induced angiogenesis in ewing sarcoma cells works through bFGF production and is dependent on caveolin-1. PLoS ONE. 2013;8:e71449 pubmed publisher
  8. Park J, Son A, Hua R, Wang L, Zhang X, Zhou R. Human cataract mutations in EPHA2 SAM domain alter receptor stability and function. PLoS ONE. 2012;7:e36564 pubmed publisher