This is a Validated Antibody Database (VAD) review about cow EHMT2, based on 21 published articles (read how Labome selects the articles), using EHMT2 antibody in all methods. It is aimed to help Labome visitors find the most suited EHMT2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EHMT2 synonym: BAT8; histone-lysine N-methyltransferase EHMT2; ankyrin repeat-containing protein; euchromatic histone-lysine N-methyltransferase 2; histone-lysine N-methyltransferase, H3 lysine-9 specific 3

Invitrogen
rabbit monoclonal (B.133.9)
  • western blot; human; loading ...; fig 2c
In order to study how methylation of p53 affects its interactions with other proteins such as PUMA and p300/CBP, Invitrogen EHMT2 antibody (Thermo Fisher, MA5-14880) was used in western blot on human samples (fig 2c). Oncogene (2017) ncbi
rabbit monoclonal (B.133.9)
  • western blot; human; fig 3
In order to assess the necessity of S-adenosyl methionine for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3, Invitrogen EHMT2 antibody (Thermo Fisher, MA5-14880) was used in western blot on human samples (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (C6H3)
  • immunohistochemistry - frozen section; mouse; loading ...; fig s3b
  • flow cytometry; mouse; loading ...; fig s3b
Cell Signaling Technology EHMT2 antibody (Cell Signaling, C6H3) was used in immunohistochemistry - frozen section on mouse samples (fig s3b) and in flow cytometry on mouse samples (fig s3b). J Cell Biol (2018) ncbi
rabbit monoclonal (C6H3)
  • western blot; human; 1:1000; loading ...; fig 1a
Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in western blot on human samples at 1:1000 (fig 1a). Nat Commun (2017) ncbi
rabbit monoclonal (C6H3)
  • immunohistochemistry - paraffin section; pig; 1:100; loading ...; fig s3b
In order to elucidate the inital stage of developmental events from human primordial germ cells, Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in immunohistochemistry - paraffin section on pig samples at 1:100 (fig s3b). Nature (2017) ncbi
rabbit monoclonal (C6H3)
  • immunoprecipitation; human; 1:1000; loading ...; fig 4g
  • western blot; human; 1:1000; loading ...; fig 4f
Cell Signaling Technology EHMT2 antibody (Cell Signaling Technology, 3306S) was used in immunoprecipitation on human samples at 1:1000 (fig 4g) and in western blot on human samples at 1:1000 (fig 4f). Mol Cell (2017) ncbi
rabbit monoclonal (C6H3)
  • western blot; mouse; fig 2
  • western blot; human; fig 3
Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306S) was used in western blot on mouse samples (fig 2) and in western blot on human samples (fig 3). Sci Rep (2016) ncbi
rabbit monoclonal (C6H3)
  • western blot; zebrafish ; fig 1
In order to report that ZNF644 is a co-regulator of G9a/H3K9me2-mediated gene silencing during neuronal differentiation, Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in western blot on zebrafish samples (fig 1). Stem Cell Reports (2016) ncbi
rabbit monoclonal (C6H3)
  • immunoprecipitation; mouse; fig 6
  • immunocytochemistry; mouse; fig 6
  • western blot; mouse; fig 1
Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306S) was used in immunoprecipitation on mouse samples (fig 6), in immunocytochemistry on mouse samples (fig 6) and in western blot on mouse samples (fig 1). Nucleic Acids Res (2016) ncbi
rabbit monoclonal (C6H3)
  • immunocytochemistry; mouse; 1:1000; fig 5
In order to protect preimplantation mouse embryos from endogenous retrotransposons by histone chaperone CAF-1 mediating repressive histone modifications, Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in immunocytochemistry on mouse samples at 1:1000 (fig 5). Proc Natl Acad Sci U S A (2015) ncbi
rabbit monoclonal (C6H3)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 1
Cell Signaling Technology EHMT2 antibody (Cell Signaling Technology, 3306) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 1). PLoS ONE (2015) ncbi
rabbit monoclonal (C6H3)
  • chromatin immunoprecipitation; mouse; fig 6
Cell Signaling Technology EHMT2 antibody (cell Signaling Tech, 3306) was used in chromatin immunoprecipitation on mouse samples (fig 6). Nucleic Acids Res (2016) ncbi
rabbit monoclonal (C6H3)
  • western blot; human
In order to study the relationship between nuclear LASP-1 and the epigenetic machinery in breast cancer, Cell Signaling Technology EHMT2 antibody (cst, 3306S) was used in western blot on human samples . Oncogene (2016) ncbi
rabbit monoclonal (C6H3)
  • immunocytochemistry; human; 1:200
  • western blot; human; 1:1000
Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in immunocytochemistry on human samples at 1:200 and in western blot on human samples at 1:1000. Biochem Biophys Res Commun (2015) ncbi
rabbit monoclonal (C6H3)
  • western blot; human; fig 3
Cell Signaling Technology EHMT2 antibody (Cell signaling, 3306) was used in western blot on human samples (fig 3). Ann Surg Oncol (2015) ncbi
rabbit monoclonal (C6H3)
  • western blot; rat; 1:500; fig 2
Cell Signaling Technology EHMT2 antibody (Cell signalling technology, 3306) was used in western blot on rat samples at 1:500 (fig 2). J Neurosci (2015) ncbi
rabbit monoclonal (C6H3)
  • immunocytochemistry; human; 1:100; loading ...; fig 3a
In order to use the chromatin in situ proximity technique to identify chromosome 11-specific hubs enriched for H3K9me2, Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in immunocytochemistry on human samples at 1:100 (fig 3a). Epigenetics (2014) ncbi
rabbit monoclonal (C6H3)
  • immunocytochemistry; mouse
Cell Signaling Technology EHMT2 antibody (Cell Signaling Technology, 3306) was used in immunocytochemistry on mouse samples . Blood (2014) ncbi
rabbit monoclonal (C6H3)
  • western blot; mouse; 1:1000
Cell Signaling Technology EHMT2 antibody (Cell Signaling Technology, 3306) was used in western blot on mouse samples at 1:1000. Neuroscience (2014) ncbi
rabbit monoclonal (C6H3)
  • western blot; mouse; 1:1000
Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in western blot on mouse samples at 1:1000. Mol Endocrinol (2013) ncbi
rabbit monoclonal (C6H3)
  • western blot; mouse; 1:1000
Cell Signaling Technology EHMT2 antibody (Cell Signaling, 3306) was used in western blot on mouse samples at 1:1000. Neurobiol Dis (2013) ncbi
Articles Reviewed
  1. Casey A, Sinha A, Singhania R, Livingstone J, Waterhouse P, Tharmapalan P, et al. Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities. J Cell Biol. 2018;217:2951-2974 pubmed publisher
  2. Wang Y, Zhang J, Su Y, Shen Y, Jiang D, Hou Y, et al. G9a regulates breast cancer growth by modulating iron homeostasis through the repression of ferroxidase hephaestin. Nat Commun. 2017;8:274 pubmed publisher
  3. Kobayashi T, Zhang H, Tang W, Irie N, Withey S, Klisch D, et al. Principles of early human development and germ cell program from conserved model systems. Nature. 2017;546:416-420 pubmed publisher
  4. Sakamaki J, Wilkinson S, Hahn M, Tasdemir N, O Prey J, Clark W, et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell. 2017;66:517-532.e9 pubmed publisher
  5. Ow J, Palanichamy Kala M, Rao V, Choi M, Bharathy N, Taneja R. G9a inhibits MEF2C activity to control sarcomere assembly. Sci Rep. 2016;6:34163 pubmed publisher
  6. Olsen J, Wong L, Deimling S, Miles A, Guo H, Li Y, et al. G9a and ZNF644 Physically Associate to Suppress Progenitor Gene Expression during Neurogenesis. Stem Cell Reports. 2016;7:454-470 pubmed publisher
  7. Rada M, Vasileva E, Lezina L, Marouco D, Antonov A, Macip S, et al. Human EHMT2/G9a activates p53 through methylation-independent mechanism. Oncogene. 2017;36:922-932 pubmed publisher
  8. Rao V, Ow J, Shankar S, Bharathy N, Manikandan J, Wang Y, et al. G9a promotes proliferation and inhibits cell cycle exit during myogenic differentiation. Nucleic Acids Res. 2016;44:8129-43 pubmed publisher
  9. Jayaram H, Hoelper D, Jain S, Cantone N, Lundgren S, Poy F, et al. S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3. Proc Natl Acad Sci U S A. 2016;113:6182-7 pubmed publisher
  10. Hatanaka Y, Inoue K, Oikawa M, Kamimura S, Ogonuki N, Kodama E, et al. Histone chaperone CAF-1 mediates repressive histone modifications to protect preimplantation mouse embryos from endogenous retrotransposons. Proc Natl Acad Sci U S A. 2015;112:14641-6 pubmed publisher
  11. Lee J, Lee S, Heo S, Kim K, Kim C, Kim D, et al. Novel Function of Lysine Methyltransferase G9a in the Regulation of Sox2 Protein Stability. PLoS ONE. 2015;10:e0141118 pubmed publisher
  12. Akhade V, Dighe S, Kataruka S, Rao M. Mechanism of Wnt signaling induced down regulation of mrhl long non-coding RNA in mouse spermatogonial cells. Nucleic Acids Res. 2016;44:387-401 pubmed publisher
  13. Duvall Noelle N, Karwandyar A, Richmond A, Raman D. LASP-1: a nuclear hub for the UHRF1-DNMT1-G9a-Snail1 complex. Oncogene. 2016;35:1122-33 pubmed publisher
  14. Ren A, Qiu Y, Cui H, Fu G. Inhibition of H3K9 methyltransferase G9a induces autophagy and apoptosis in oral squamous cell carcinoma. Biochem Biophys Res Commun. 2015;459:10-7 pubmed publisher
  15. Hsiao S, Chen M, Chen C, Chien M, Hua K, Hsiao M, et al. The H3K9 Methyltransferase G9a Represses E-cadherin and is Associated with Myometrial Invasion in Endometrial Cancer. Ann Surg Oncol. 2015;22 Suppl 3:S1556-65 pubmed publisher
  16. Liu J, Magri L, Zhang F, Marsh N, Albrecht S, Huynh J, et al. Chromatin landscape defined by repressive histone methylation during oligodendrocyte differentiation. J Neurosci. 2015;35:352-65 pubmed publisher
  17. Chen X, Yammine S, Shi C, Tark Dame M, Göndör A, Ohlsson R. The visualization of large organized chromatin domains enriched in the H3K9me2 mark within a single chromosome in a single cell. Epigenetics. 2014;9:1439-45 pubmed publisher
  18. Hattangadi S, Martinez Morilla S, Patterson H, Shi J, Burke K, Avila Figueroa A, et al. Histones to the cytosol: exportin 7 is essential for normal terminal erythroid nuclear maturation. Blood. 2014;124:1931-40 pubmed
  19. Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  20. Jefferson W, Chevalier D, Phelps J, Cantor A, Padilla Banks E, Newbold R, et al. Persistently altered epigenetic marks in the mouse uterus after neonatal estrogen exposure. Mol Endocrinol. 2013;27:1666-77 pubmed publisher
  21. Subbanna S, Shivakumar M, Umapathy N, Saito M, Mohan P, Kumar A, et al. G9a-mediated histone methylation regulates ethanol-induced neurodegeneration in the neonatal mouse brain. Neurobiol Dis. 2013;54:475-85 pubmed publisher