This is a Validated Antibody Database (VAD) review about cow EGR1, based on 20 published articles (read how Labome selects the articles), using EGR1 antibody in all methods. It is aimed to help Labome visitors find the most suited EGR1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EGR1 synonym: early growth response protein 1; EGR-1

Knockout validation
Cell Signaling Technology
rabbit monoclonal (44D5)
  • western blot knockout validation; mouse; loading ...; fig 9b
  • immunocytochemistry; human; 1:400; loading ...; fig 7b
  • western blot; human; loading ...; fig 9d
In order to study the effect of venezuelan equine encephalitis virus on apoptosis and its mechanism, Cell Signaling Technology EGR1 antibody (Cell Signaling, 4154) was used in western blot knockout validation on mouse samples (fig 9b), in immunocytochemistry on human samples at 1:400 (fig 7b) and in western blot on human samples (fig 9d). J Virol (2016) ncbi
Invitrogen
rabbit monoclonal (T.126.1)
  • immunohistochemistry; mouse; loading ...; fig s16b
  • western blot; mouse; loading ...; fig 4e
In order to study the roll of a mammalian circular RNA locus in miRNA homeostasis and brain function, Invitrogen EGR1 antibody (Thermo Fisher, MA5-15009) was used in immunohistochemistry on mouse samples (fig s16b) and in western blot on mouse samples (fig 4e). Science (2017) ncbi
rabbit monoclonal (T.160.5)
  • immunohistochemistry - paraffin section; human; 1:50
Invitrogen EGR1 antibody (ThermoFisher Scientific, T.160.5) was used in immunohistochemistry - paraffin section on human samples at 1:50. Diagn Pathol (2014) ncbi
Cell Signaling Technology
rabbit monoclonal (44D5)
  • immunohistochemistry; mouse; 1:800; loading ...; fig s11d
Cell Signaling Technology EGR1 antibody (Cell Signaling, 4154) was used in immunohistochemistry on mouse samples at 1:800 (fig s11d). Science (2018) ncbi
rabbit monoclonal (44D5)
  • western blot; mouse; 1:1000; loading ...; fig 3g
Cell Signaling Technology EGR1 antibody (Cell Signaling, 4154) was used in western blot on mouse samples at 1:1000 (fig 3g). J Biol Chem (2018) ncbi
rabbit monoclonal (15F7)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 4b
Cell Signaling Technology EGR1 antibody (Cell Signaling Technology, 4153) was used in immunohistochemistry on mouse samples at 1:100 (fig 4b). Proc Natl Acad Sci U S A (2017) ncbi
rabbit monoclonal (15F7)
  • western blot; human; 1:1000; loading ...; fig 2d
Cell Signaling Technology EGR1 antibody (Cell Signaling, 4153S) was used in western blot on human samples at 1:1000 (fig 2d). PLoS ONE (2017) ncbi
rabbit monoclonal (15F7)
  • western blot; human; 1:1000; loading ...; fig s6c
Cell Signaling Technology EGR1 antibody (CST, 4153) was used in western blot on human samples at 1:1000 (fig s6c). Oncogene (2017) ncbi
rabbit monoclonal (15F7)
  • western blot; human; loading ...; fig 6g
Cell Signaling Technology EGR1 antibody (Cell Signaling, 4153) was used in western blot on human samples (fig 6g). Oncotarget (2016) ncbi
rabbit monoclonal (15F7)
  • chromatin immunoprecipitation; human; fig s2
  • western blot; human; 1:1000; fig s2
Cell Signaling Technology EGR1 antibody (Cell Signaling Tech, 4153) was used in chromatin immunoprecipitation on human samples (fig s2) and in western blot on human samples at 1:1000 (fig s2). Nat Commun (2016) ncbi
rabbit monoclonal (15F7)
  • chromatin immunoprecipitation; human; 1:40; fig 3
Cell Signaling Technology EGR1 antibody (Cell Signaling Tech, 15F7) was used in chromatin immunoprecipitation on human samples at 1:40 (fig 3). Nat Commun (2016) ncbi
rabbit monoclonal (15F7)
  • western blot; mouse; 1:1000; tbl 1
In order to study novelty-induced and brain-specific basal alternations in PI3K-Akt and MAPK/ERK signaling in a middle-aged A-betaPP/PS1 mouse model of Alzheimer's disease, Cell Signaling Technology EGR1 antibody (CST, 4153) was used in western blot on mouse samples at 1:1000 (tbl 1). J Alzheimers Dis (2016) ncbi
rabbit monoclonal (44D5)
  • western blot knockout validation; mouse; loading ...; fig 9b
  • immunocytochemistry; human; 1:400; loading ...; fig 7b
  • western blot; human; loading ...; fig 9d
In order to study the effect of venezuelan equine encephalitis virus on apoptosis and its mechanism, Cell Signaling Technology EGR1 antibody (Cell Signaling, 4154) was used in western blot knockout validation on mouse samples (fig 9b), in immunocytochemistry on human samples at 1:400 (fig 7b) and in western blot on human samples (fig 9d). J Virol (2016) ncbi
rabbit monoclonal (15F7)
  • chromatin immunoprecipitation; human; fig s3
Cell Signaling Technology EGR1 antibody (Cell Signaling Techn, 4153) was used in chromatin immunoprecipitation on human samples (fig s3). BMC Cell Biol (2015) ncbi
rabbit monoclonal (15F7)
  • western blot; human; fig 8a
Cell Signaling Technology EGR1 antibody (Cell signaling, 4153) was used in western blot on human samples (fig 8a). Biomolecules (2015) ncbi
rabbit monoclonal (15F7)
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 7
In order to elucidate role of androgen signaling in beta-catenin-initiated prostate tumorigenesis, Cell Signaling Technology EGR1 antibody (Cell Signaling, 4153) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 7). Oncogene (2016) ncbi
rabbit monoclonal (15F7)
  • immunohistochemistry - paraffin section; rat; 1:200
Cell Signaling Technology EGR1 antibody (Cell Signaling, #4153) was used in immunohistochemistry - paraffin section on rat samples at 1:200. Neuroscience (2015) ncbi
rabbit monoclonal (15F7)
  • western blot; human; fig 5
Cell Signaling Technology EGR1 antibody (Cell Signaling, 4153) was used in western blot on human samples (fig 5). Sci Rep (2015) ncbi
rabbit monoclonal (15F7)
  • western blot; human; fig  3
Cell Signaling Technology EGR1 antibody (Cell Signaling, 4153) was used in western blot on human samples (fig  3). J Biol Chem (2015) ncbi
rabbit monoclonal (15F7)
  • immunohistochemistry; rat; 1:250
Cell Signaling Technology EGR1 antibody (Cell Signalling Technology, 15F7) was used in immunohistochemistry on rat samples at 1:250. J Chem Neuroanat (2014) ncbi
rabbit monoclonal (15F7)
  • immunohistochemistry - frozen section; human; 1:50; fig 4
In order to analyze developing cerebral cortex and low-coverage single-cell mRNA sequencing that reveals activated signaling pathways and cellular heterogeneity, Cell Signaling Technology EGR1 antibody (Cell signaling, 41535) was used in immunohistochemistry - frozen section on human samples at 1:50 (fig 4). Nat Biotechnol (2014) ncbi
Articles Reviewed
  1. Bugaj L, Sabnis A, Mitchell A, Garbarino J, Toettcher J, Bivona T, et al. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science. 2018;361: pubmed publisher
  2. Choi S, Neequaye P, French S, Gonzalez F, Gyamfi M. Pregnane X receptor promotes ethanol-induced hepatosteatosis in mice. J Biol Chem. 2018;293:1-17 pubmed publisher
  3. Piwecka M, Glažar P, Hernández Miranda L, Memczak S, Wolf S, Rybak Wolf A, et al. Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function. Science. 2017;357: pubmed publisher
  4. Oh J, Wang Y, Chen S, Li P, Du N, Yu Z, et al. Genetic background-dependent role of Egr1 for eyelid development. Proc Natl Acad Sci U S A. 2017;114:E7131-E7139 pubmed publisher
  5. Laporte A, Barrott J, Yao R, Poulin N, Brodin B, Jones K, et al. HDAC and Proteasome Inhibitors Synergize to Activate Pro-Apoptotic Factors in Synovial Sarcoma. PLoS ONE. 2017;12:e0169407 pubmed publisher
  6. Kesarwani A, Ramirez O, Gupta A, Yang X, Murthy T, Minella A, et al. Cancer-associated SF3B1 mutants recognize otherwise inaccessible cryptic 3' splice sites within RNA secondary structures. Oncogene. 2017;36:1123-1133 pubmed publisher
  7. Laporte A, Ji J, Ma L, Nielsen T, Brodin B. Identification of cytotoxic agents disrupting synovial sarcoma oncoprotein interactions by proximity ligation assay. Oncotarget. 2016;7:34384-94 pubmed publisher
  8. Wu S, Rupaimoole R, Shen F, Pradeep S, Pecot C, Ivan C, et al. A miR-192-EGR1-HOXB9 regulatory network controls the angiogenic switch in cancer. Nat Commun. 2016;7:11169 pubmed publisher
  9. Weigel C, Veldwijk M, Oakes C, Seibold P, Slynko A, Liesenfeld D, et al. Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis. Nat Commun. 2016;7:10893 pubmed publisher
  10. Guillot F, Kemppainen S, Lavasseur G, Miettinen P, Laroche S, Tanila H, et al. Brain-Specific Basal and Novelty-Induced Alternations in PI3K-Akt and MAPK/ERK Signaling in a Middle-Aged AβPP/PS1 Mouse Model of Alzheimer's Disease. J Alzheimers Dis. 2016;51:1157-73 pubmed publisher
  11. Baer A, Lundberg L, Swales D, Waybright N, Pinkham C, Dinman J, et al. Venezuelan Equine Encephalitis Virus Induces Apoptosis through the Unfolded Protein Response Activation of EGR1. J Virol. 2016;90:3558-72 pubmed publisher
  12. Ponti D, Bastianelli D, Rosa P, Pacini L, Ibrahim M, Rendina E, et al. The expression of B23 and EGR1 proteins is functionally linked in tumor cells under stress conditions. BMC Cell Biol. 2015;16:27 pubmed publisher
  13. Sobolewski C, Sanduja S, Blanco F, Hu L, Dixon D. Histone Deacetylase Inhibitors Activate Tristetraprolin Expression through Induction of Early Growth Response Protein 1 (EGR1) in Colorectal Cancer Cells. Biomolecules. 2015;5:2035-55 pubmed publisher
  14. Lee S, Luong R, Johnson D, Cunha G, Rivina L, Gonzalgo M, et al. Androgen signaling is a confounding factor for β-catenin-mediated prostate tumorigenesis. Oncogene. 2016;35:702-14 pubmed publisher
  15. Liu W, Crews F. Adolescent intermittent ethanol exposure enhances ethanol activation of the nucleus accumbens while blunting the prefrontal cortex responses in adult rat. Neuroscience. 2015;293:92-108 pubmed publisher
  16. Kobayashi K, Sakurai K, Hiramatsu H, Inada K, Shiogama K, Nakamura S, et al. The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines. Sci Rep. 2015;5:8428 pubmed publisher
  17. Dong A, Wodziak D, Lowe A. Epidermal growth factor receptor (EGFR) signaling requires a specific endoplasmic reticulum thioredoxin for the post-translational control of receptor presentation to the cell surface. J Biol Chem. 2015;290:8016-27 pubmed publisher
  18. Vogels R, Vlenterie M, Versleijen Jonkers Y, Ruijter E, Bekers E, Verdijk M, et al. Solitary fibrous tumor - clinicopathologic, immunohistochemical and molecular analysis of 28 cases. Diagn Pathol. 2014;9:224 pubmed publisher
  19. Hoefflin S, Carter D. Neuronal expression of SOX2 is enriched in specific hypothalamic cell groups. J Chem Neuroanat. 2014;61-62:153-60 pubmed publisher
  20. Pollen A, Nowakowski T, Shuga J, Wang X, Leyrat A, Lui J, et al. Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex. Nat Biotechnol. 2014;32:1053-8 pubmed publisher