This is a Validated Antibody Database (VAD) review about cow DNMT3A, based on 13 published articles (read how Labome selects the articles), using DNMT3A antibody in all methods. It is aimed to help Labome visitors find the most suited DNMT3A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
DNMT3A synonym: DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha

Knockout validation
Cell Signaling Technology
rabbit monoclonal (D23G1)
  • western blot; human; 1:2000; loading ...; fig 1b
  • immunocytochemistry knockout validation; mouse; 1:400; loading ...; fig 6c
Cell Signaling Technology DNMT3A antibody (Cell Signaling, D23G1/3598) was used in western blot on human samples at 1:2000 (fig 1b) and in immunocytochemistry knockout validation on mouse samples at 1:400 (fig 6c). J Clin Invest (2017) ncbi
Cell Signaling Technology
rabbit polyclonal
  • western blot; human; 1:2000; fig 4b
  • western blot knockout validation; mouse; 1:2000; fig s1a
In order to study how DNMT3A is involved in the function of PML-RARA, RUNX1-RUNX1T1, and MLL-AF9, Cell Signaling Technology DNMT3A antibody (Cell Signaling, 2160) was used in western blot on human samples at 1:2000 (fig 4b) and in western blot knockout validation on mouse samples at 1:2000 (fig s1a). J Clin Invest (2016) ncbi
Cell Signaling Technology
rabbit monoclonal (D23G1)
  • western blot; mouse; 1:1000; fig 3e
Cell Signaling Technology DNMT3A antibody (Cell Signaling, D23G1) was used in western blot on mouse samples at 1:1000 (fig 3e). Exp Hematol (2018) ncbi
rabbit monoclonal (D23G1)
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig 2a
Cell Signaling Technology DNMT3A antibody (CST, 3598) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 2a). Sci Rep (2018) ncbi
rabbit monoclonal (D23G1)
  • western blot; human; 1:2000; loading ...; fig 1b
  • immunocytochemistry knockout validation; mouse; 1:400; loading ...; fig 6c
Cell Signaling Technology DNMT3A antibody (Cell Signaling, D23G1/3598) was used in western blot on human samples at 1:2000 (fig 1b) and in immunocytochemistry knockout validation on mouse samples at 1:400 (fig 6c). J Clin Invest (2017) ncbi
rabbit monoclonal (D23G1)
  • western blot; human; fig 4g
In order to understand the role of nicotinamide metabolism in the maintenance of glioblastoma stem cells, Cell Signaling Technology DNMT3A antibody (Cell signaling, 3598) was used in western blot on human samples (fig 4g). JCI Insight (2017) ncbi
rabbit monoclonal (D23G1)
  • western blot; human; fig 4a
Cell Signaling Technology DNMT3A antibody (Cell Signaling, 3598) was used in western blot on human samples (fig 4a). Biochem Biophys Res Commun (2017) ncbi
rabbit monoclonal (D23G1)
  • immunoprecipitation; human; 1:50; fig 6b
In order to assess the ability of TGF-beta to alter DNA methyltransferase expression in primary lung fibroblasts, Cell Signaling Technology DNMT3A antibody (Cell Signaling, 3598) was used in immunoprecipitation on human samples at 1:50 (fig 6b). J Biol Chem (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:2000; fig 4b
  • western blot knockout validation; mouse; 1:2000; fig s1a
In order to study how DNMT3A is involved in the function of PML-RARA, RUNX1-RUNX1T1, and MLL-AF9, Cell Signaling Technology DNMT3A antibody (Cell Signaling, 2160) was used in western blot on human samples at 1:2000 (fig 4b) and in western blot knockout validation on mouse samples at 1:2000 (fig s1a). J Clin Invest (2016) ncbi
rabbit monoclonal (D23G1)
  • western blot; human; 1:500; loading ...; fig 1a, 2a
Cell Signaling Technology DNMT3A antibody (Active Motif, 3598) was used in western blot on human samples at 1:500 (fig 1a, 2a). Oncotarget (2015) ncbi
rabbit polyclonal
  • western blot; mouse
  • western blot; human
Cell Signaling Technology DNMT3A antibody (cell signaling, 2160S) was used in western blot on mouse samples and in western blot on human samples . Nat Genet (2015) ncbi
rabbit polyclonal
  • western blot; mouse; fig s9
In order to elucidate the function of miR-29a in hematopoietic stem and progenitor cells, Cell Signaling Technology DNMT3A antibody (Cell Signaling, 2160S) was used in western blot on mouse samples (fig s9). Blood (2015) ncbi
rabbit monoclonal (D23G1)
  • western blot; human
Cell Signaling Technology DNMT3A antibody (Cell signaling, D23G1) was used in western blot on human samples . Nucleic Acids Res (2014) ncbi
rabbit monoclonal (D23G1)
  • western blot; mouse; 1:1000
Cell Signaling Technology DNMT3A antibody (Cell Signaling Technology, 3598) was used in western blot on mouse samples at 1:1000. Neuroscience (2014) ncbi
rabbit monoclonal (D23G1)
  • western blot; human; 1:3000
Cell Signaling Technology DNMT3A antibody (Cell Signaling, 3598) was used in western blot on human samples at 1:3000. Exp Hematol (2012) ncbi
Articles Reviewed
  1. Shi X, Kitano A, Jiang Y, Luu V, Hoegenauer K, Nakada D. Clonal expansion and myeloid leukemia progression modeled by multiplex gene editing of murine hematopoietic progenitor cells. Exp Hematol. 2018;64:33-44.e5 pubmed publisher
  2. Gong Z, Zhou Q. Dnmt3a in the dorsal dentate gyrus is a key regulator of fear renewal. Sci Rep. 2018;8:5093 pubmed publisher
  3. Cole C, Russler Germain D, Ketkar S, Verdoni A, Smith A, Bangert C, et al. Haploinsufficiency for DNA methyltransferase 3A predisposes hematopoietic cells to myeloid malignancies. J Clin Invest. 2017;127:3657-3674 pubmed publisher
  4. Jung J, Kim L, Wang X, Wu Q, Sanvoranart T, Hubert C, et al. Nicotinamide metabolism regulates glioblastoma stem cell maintenance. JCI Insight. 2017;2: pubmed publisher
  5. Du M, Zhang Y, Mao Y, Mou J, Zhao J, Xue Q, et al. MiR-33a suppresses proliferation of NSCLC cells via targeting METTL3 mRNA. Biochem Biophys Res Commun. 2017;482:582-589 pubmed publisher
  6. Koh H, Scruggs A, Huang S. Transforming Growth Factor-β1 Increases DNA Methyltransferase 1 and 3a Expression through Distinct Post-transcriptional Mechanisms in Lung Fibroblasts. J Biol Chem. 2016;291:19287-98 pubmed publisher
  7. Cole C, Verdoni A, Ketkar S, Leight E, Russler Germain D, Lamprecht T, et al. PML-RARA requires DNA methyltransferase 3A to initiate acute promyelocytic leukemia. J Clin Invest. 2016;126:85-98 pubmed publisher
  8. Vispé S, Deroide A, Davoine E, Desjobert C, Lestienne F, Fournier L, et al. Consequences of combining siRNA-mediated DNA methyltransferase 1 depletion with 5-aza-2'-deoxycytidine in human leukemic KG1 cells. Oncotarget. 2015;6:15265-82 pubmed
  9. Liao J, Karnik R, Gu H, Ziller M, Clement K, Tsankov A, et al. Targeted disruption of DNMT1, DNMT3A and DNMT3B in human embryonic stem cells. Nat Genet. 2015;47:469-78 pubmed publisher
  10. Hu W, Dooley J, Chung S, Chandramohan D, Cimmino L, Mukherjee S, et al. miR-29a maintains mouse hematopoietic stem cell self-renewal by regulating Dnmt3a. Blood. 2015;125:2206-16 pubmed publisher
  11. Cho S, Park J, Kang Y. AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the androgen receptor gene. Nucleic Acids Res. 2014;42:13545-56 pubmed publisher
  12. Subbanna S, Nagre N, Shivakumar M, Umapathy N, Psychoyos D, Basavarajappa B. Ethanol induced acetylation of histone at G9a exon1 and G9a-mediated histone H3 dimethylation leads to neurodegeneration in neonatal mice. Neuroscience. 2014;258:422-32 pubmed publisher
  13. Valdez B, Nieto Y, Murray D, Li Y, Wang G, Champlin R, et al. Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines. Exp Hematol. 2012;40:800-10 pubmed publisher