This is a Validated Antibody Database (VAD) review about cow CYLD, based on 7 published articles (read how Labome selects the articles), using CYLD antibody in all methods. It is aimed to help Labome visitors find the most suited CYLD antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
CYLD synonym: ubiquitin carboxyl-terminal hydrolase CYLD; deubiquitinating enzyme CYLD; ubiquitin thioesterase CYLD; ubiquitin thiolesterase CYLD; ubiquitin-specific-processing protease CYLD

Novus Biologicals
rabbit polyclonal
  • immunocytochemistry; human; 10 ug/ml; fig 4c
  • western blot; human; fig 4d
Novus Biologicals CYLD antibody (Novus, NB110-95574) was used in immunocytochemistry on human samples at 10 ug/ml (fig 4c) and in western blot on human samples (fig 4d). J Biol Chem (2017) ncbi
Cell Signaling Technology
rabbit monoclonal (D1A10)
  • western blot; human; 1:1000; fig s15
Cell Signaling Technology CYLD antibody (Cell Signaling, 8462) was used in western blot on human samples at 1:1000 (fig s15). Nat Commun (2016) ncbi
rabbit monoclonal (D1A10)
  • western blot; human; fig 5
Cell Signaling Technology CYLD antibody (Cell Signaling, 8462) was used in western blot on human samples (fig 5). EMBO J (2016) ncbi
rabbit monoclonal (D1A10)
  • western blot; human; 1:1000; fig s11
Cell Signaling Technology CYLD antibody (Cell Signaling, 8462S) was used in western blot on human samples at 1:1000 (fig s11). Nat Commun (2016) ncbi
rabbit monoclonal (D1A10)
  • western blot; human
Cell Signaling Technology CYLD antibody (Cell Signaling, 8462) was used in western blot on human samples . J Biol Chem (2016) ncbi
rabbit monoclonal (D1A10)
  • western blot; human; fig 3, 4, 5
In order to analyze SUMOylation in neuroblastoma cells as it impairs deubiquitinating activity of CYLD, Cell Signaling Technology CYLD antibody (Cell Signaling, D1A10) was used in western blot on human samples (fig 3, 4, 5). Oncogene (2015) ncbi
rabbit monoclonal (D1A10)
  • western blot; human; 1:1000; fig 2b
Cell Signaling Technology CYLD antibody (Cell Signaling, 8462S) was used in western blot on human samples at 1:1000 (fig 2b). Nat Chem Biol (2014) ncbi
Articles Reviewed
  1. Wu G, Mu T, Gao Z, Wang J, Sy M, Li C. Prion protein is required for tumor necrosis factor α (TNFα)-triggered nuclear factor κB (NF-κB) signaling and cytokine production. J Biol Chem. 2017;292:18747-18759 pubmed publisher
  2. Nakazawa S, Oikawa D, Ishii R, Ayaki T, Takahashi H, Takeda H, et al. Linear ubiquitination is involved in the pathogenesis of optineurin-associated amyotrophic lateral sclerosis. Nat Commun. 2016;7:12547 pubmed publisher
  3. Wagner S, Satpathy S, Beli P, Choudhary C. SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes. EMBO J. 2016;35:1868-84 pubmed publisher
  4. Lin W, Zhang J, Lin H, Li Z, Sun X, Xin D, et al. Syndecan-4 negatively regulates antiviral signalling by mediating RIG-I deubiquitination via CYLD. Nat Commun. 2016;7:11848 pubmed publisher
  5. Monian P, Jiang X. The Cellular Apoptosis Susceptibility Protein (CAS) Promotes Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL)-induced Apoptosis and Cell Proliferation. J Biol Chem. 2016;291:2379-88 pubmed publisher
  6. Kobayashi T, Masoumi K, Massoumi R. Deubiquitinating activity of CYLD is impaired by SUMOylation in neuroblastoma cells. Oncogene. 2015;34:2251-60 pubmed publisher
  7. Liang Q, Dexheimer T, Zhang P, Rosenthal A, Villamil M, You C, et al. A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses. Nat Chem Biol. 2014;10:298-304 pubmed publisher