This is a Validated Antibody Database (VAD) review about cow ATP1A1, based on 14 published articles (read how Labome selects the articles), using ATP1A1 antibody in all methods. It is aimed to help Labome visitors find the most suited ATP1A1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ATP1A1 synonym: sodium/potassium-transporting ATPase subunit alpha-1; ATPase, Na+/K+ transporting, alpha 1 polypeptide; Na(+)/K(+) ATPase alpha-1 subunit; sodium pump subunit alpha-1

Novus Biologicals
mouse monoclonal (464.6)
  • immunohistochemistry; human; 1:100; fig 5A
In order to establish an application to generate retinal pigmented epithelium from induced pluripotent stem cells, Novus Biologicals ATP1A1 antibody (Novus, NB300-146) was used in immunohistochemistry on human samples at 1:100 (fig 5A). PLoS ONE (2017) ncbi
mouse monoclonal (464.6)
  • immunocytochemistry; human; loading ...; fig 2c
In order to show that apical polarization of the sodium, potassium-ATPase in retinal pigment epithelium cells depends on the expression of the beta2 subunit, Novus Biologicals ATP1A1 antibody (Novus, NB300-146) was used in immunocytochemistry on human samples (fig 2c). Front Physiol (2016) ncbi
mouse monoclonal (464.6)
  • western blot; cow; 1:5000; loading ...; tbl 2
In order to study the transport of cholesterol and phospholipids in mammary epithelial cells, Novus Biologicals ATP1A1 antibody (Novus Biologicals, NB300-146) was used in western blot on cow samples at 1:5000 (tbl 2). Mol Cell Endocrinol (2017) ncbi
mouse monoclonal (464.6)
  • immunocytochemistry; mouse; 1:1000; fig 1
In order to study a reconstituted choroid plexus epithelium to show T-lymphocytes traffic into the brain across the blood-CSF barrier, Novus Biologicals ATP1A1 antibody (Novus Biologicals, NB300-146) was used in immunocytochemistry on mouse samples at 1:1000 (fig 1). PLoS ONE (2016) ncbi
mouse monoclonal (464.6)
  • immunohistochemistry - frozen section; human; 1:100; fig 9
In order to assess the lateral wall of the cochlea and connexin26/30 proteins and separate expression in man by using super-resolution structured illumination fluorescence microscopy, Novus Biologicals ATP1A1 antibody (Novus, NB300-146) was used in immunohistochemistry - frozen section on human samples at 1:100 (fig 9). Cell Tissue Res (2016) ncbi
mouse monoclonal (464.6)
  • western blot; human
In order to study retinal degeneration caused by disruption of the microtubule-binding and membrane-binding domains of CEP290, Novus Biologicals ATP1A1 antibody (Novus, NB300-146) was used in western blot on human samples . J Clin Invest (2013) ncbi
Invitrogen
mouse monoclonal (M7-PB-E9)
  • western blot; human; loading ...; fig 3c
In order to show that PHB1 is highly expressed in non-small cell lung cancers patients and correlates with poor survival, Invitrogen ATP1A1 antibody (Thermo Fisher, MA3-928) was used in western blot on human samples (fig 3c). Oncogene (2017) ncbi
mouse monoclonal (M7-PB-E9)
  • western blot; human; 1:500; fig 1, 2
In order to study seizure management by subcellular localization of GABA transporters, Invitrogen ATP1A1 antibody (Affinity Bio reagents, MA3-928) was used in western blot on human samples at 1:500 (fig 1, 2). Neurochem Res (2015) ncbi
mouse monoclonal (464.6)
  • immunocytochemistry; mouse; 1:200
In order to analyze regulation of Rab27a localisation on melanosomes by Na+,K+-ATPase alpha1, Invitrogen ATP1A1 antibody (Thermo Fisher Scientific, MA1-16731) was used in immunocytochemistry on mouse samples at 1:200. PLoS ONE (2014) ncbi
mouse monoclonal (M7-PB-E9)
  • immunocytochemistry; human; 1:50; tbl 2
In order to study of Flotillin-2 expression from a cell model to human tissue in health and inflammatory bowel disease, Invitrogen ATP1A1 antibody (ABR Affinity BioReagents, MA3-928) was used in immunocytochemistry on human samples at 1:50 (tbl 2). Int J Med Sci (2013) ncbi
EMD Millipore
rabbit polyclonal
  • western blot; mouse; fig 6
In order to determine the major role for alveolar epithelial type 1 cells in alveolar fluid clearance revealed by knockout mice, EMD Millipore ATP1A1 antibody (Millipore, 06-520) was used in western blot on mouse samples (fig 6). Am J Respir Cell Mol Biol (2016) ncbi
rabbit polyclonal
  • western blot; human; 1:10,000; fig 1
In order to study epithelial cells with stable expression of SLC26A9 Cl-channels by generation and functional characterization, EMD Millipore ATP1A1 antibody (Millipore, 06-520) was used in western blot on human samples at 1:10,000 (fig 1). Am J Physiol Lung Cell Mol Physiol (2016) ncbi
rabbit polyclonal
  • immunocytochemistry; human; fig 3
EMD Millipore ATP1A1 antibody (Upstate, 06-520) was used in immunocytochemistry on human samples (fig 3). Biochem Cell Biol (2014) ncbi
rabbit polyclonal
  • western blot; mouse; 1:800; loading ...; fig 4a
EMD Millipore ATP1A1 antibody (Millipore, 06-520) was used in western blot on mouse samples at 1:800 (fig 4a). Eur J Neurosci (2014) ncbi
Articles Reviewed
  1. Yurugi H, Marini F, Weber C, David K, Zhao Q, Binder H, et al. Targeting prohibitins with chemical ligands inhibits KRAS-mediated lung tumours. Oncogene. 2017;36:4778-4789 pubmed publisher
  2. Geng Z, Walsh P, Truong V, Hill C, Ebeling M, Kapphahn R, et al. Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration. PLoS ONE. 2017;12:e0173575 pubmed publisher
  3. Lobato Álvarez J, Roldán M, López Murillo T, González Ramírez R, Bonilla Delgado J, Shoshani L. The Apical Localization of Na+, K+-ATPase in Cultured Human Retinal Pigment Epithelial Cells Depends on Expression of the ?2 Subunit. Front Physiol. 2016;7:450 pubmed
  4. Ontsouka C, Huang X, Aliyev E, Albrecht C. In vitro characterization and endocrine regulation of cholesterol and phospholipid transport in the mammary gland. Mol Cell Endocrinol. 2017;439:35-45 pubmed publisher
  5. Flodby P, Kim Y, Beard L, Gao D, Ji Y, Kage H, et al. Knockout Mice Reveal a Major Role for Alveolar Epithelial Type I Cells in Alveolar Fluid Clearance. Am J Respir Cell Mol Biol. 2016;55:395-406 pubmed publisher
  6. Strazielle N, Creidy R, Malcus C, Boucraut J, Ghersi Egea J. T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium. PLoS ONE. 2016;11:e0150945 pubmed publisher
  7. Liu W, Edin F, Blom H, Magnusson P, Schrott Fischer A, Glueckert R, et al. Super-resolution structured illumination fluorescence microscopy of the lateral wall of the cochlea: the Connexin26/30 proteins are separately expressed in man. Cell Tissue Res. 2016;365:13-27 pubmed publisher
  8. Salomon J, Spahn S, Wang X, Füllekrug J, Bertrand C, Mall M. Generation and functional characterization of epithelial cells with stable expression of SLC26A9 Cl- channels. Am J Physiol Lung Cell Mol Physiol. 2016;310:L593-602 pubmed publisher
  9. Madsen K, Hansen G, Danielsen E, Schousboe A. The subcellular localization of GABA transporters and its implication for seizure management. Neurochem Res. 2015;40:410-9 pubmed publisher
  10. Hu Q, Wu Y, Tang J, Zheng W, Wang Q, Nahirney D, et al. Expression of polycystins and fibrocystin on primary cilia of lung cells. Biochem Cell Biol. 2014;92:547-54 pubmed publisher
  11. Booth A, Tarafder A, Hume A, Recchi C, Seabra M. A role for Na+,K+-ATPase ?1 in regulating Rab27a localisation on melanosomes. PLoS ONE. 2014;9:e102851 pubmed publisher
  12. Zhao C, Zhang J, Li K, Yang J, Yu H, Duan S, et al. ?-Catenin regulates membrane potential in muscle cells by regulating the ?2 subunit of Na,K-ATPase. Eur J Neurosci. 2014;40:2216-24 pubmed publisher
  13. Drivas T, Holzbaur E, Bennett J. Disruption of CEP290 microtubule/membrane-binding domains causes retinal degeneration. J Clin Invest. 2013;123:4525-39 pubmed publisher
  14. Gauss A, Buchholz I, Zahn A, Schmitz G, Stremmel W, Fuellekrug J, et al. Flotillin-2 expression in the human gut: from a cell model to human tissue in health and inflammatory bowel diseases. Int J Med Sci. 2013;10:1259-70 pubmed publisher