This is a Validated Antibody Database (VAD) review about cow AP2A1, based on 15 published articles (read how Labome selects the articles), using AP2A1 antibody in all methods. It is aimed to help Labome visitors find the most suited AP2A1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
AP2A1 synonym: AP-2 complex subunit alpha-1; adaptor related protein complex 2 alpha 1 subunit

Invitrogen
mouse monoclonal (AC1-M11)
  • western blot; mouse; loading ...; fig 3a
Invitrogen AP2A1 antibody (Dianova, MA3-061) was used in western blot on mouse samples (fig 3a). Neuroscience (2018) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; loading ...; fig 3d
In order to investigate the adhesion structures in 3-D cell migration, Invitrogen AP2A1 antibody (ThermoFisher, AP6) was used in immunocytochemistry on human samples (fig 3d). Science (2017) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; fig 2b
  • western blot; human; fig 1b
  • immunocytochemistry; pig; loading ...; fig 5d
  • western blot; pig; loading ...; fig 5a
In order to study amyloid precursor protein Tyr phosphorylation in mouse model of Alzheimer's disease, Invitrogen AP2A1 antibody (Thermo Fisher, MA1-064) was used in immunocytochemistry on human samples (fig 2b), in western blot on human samples (fig 1b), in immunocytochemistry on pig samples (fig 5d) and in western blot on pig samples (fig 5a). Front Mol Neurosci (2017) ncbi
mouse monoclonal (AC1-M11)
  • immunoprecipitation; human; fig s1a
  • western blot; human; fig s1a
In order to elucidate how adaptor protein 2 complexes regulate clathrin-coated pit assembly, Invitrogen AP2A1 antibody (Thermo Fisher, AC1-M11) was used in immunoprecipitation on human samples (fig s1a) and in western blot on human samples (fig s1a). J Cell Biol (2017) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; loading ...; fig 2d
In order to assess the effect of three alpha-tubulin acetyltransferase 1-specific shRNAs on proliferation and morphology in HeLa and A549 cell lines, Invitrogen AP2A1 antibody (Thermo Fisher, MA1-064) was used in immunocytochemistry on human samples (fig 2d). Cell Death Discov (2016) ncbi
mouse monoclonal (AC1-M11)
  • immunocytochemistry; human; loading ...; fig 5a
  • western blot; human; loading ...; fig 4b
In order to propose that ikarugamycin is a useful reagent to study routes of endocytic trafficking, Invitrogen AP2A1 antibody (Thermo Scientific, MA3-061) was used in immunocytochemistry on human samples (fig 5a) and in western blot on human samples (fig 4b). Traffic (2016) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; fig s5
In order to monitor the interaction beteween integrin alpha chain and AP2 and how selective integrin endocytosis drives the interaction, Invitrogen AP2A1 antibody (Pierce, MA1-064) was used in immunocytochemistry on human samples (fig s5). Nat Struct Mol Biol (2016) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; rat; fig 4
In order to characterize inhibition of clathrin-mediated and bulk endocytosis by synaptotagmin-11, Invitrogen AP2A1 antibody (Thermo Scientific, MA1-064) was used in immunocytochemistry on rat samples (fig 4). EMBO Rep (2016) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; African green monkey; fig s2d
  • western blot; human; fig s1f
In order to identify and characterize a new endocytic route that is called fast endophilin-mediated endocytosis, Invitrogen AP2A1 antibody (Thermo, MA1-064) was used in immunocytochemistry on African green monkey samples (fig s2d) and in western blot on human samples (fig s1f). Nature (2015) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; mouse; fig 2
In order to study the off-target activity of dynamin inhibitors using cells deleted for dynamins 1-3, Invitrogen AP2A1 antibody (Affinity Bioreagents, AP6) was used in immunocytochemistry on mouse samples (fig 2). J Cell Sci (2013) ncbi
mouse monoclonal (AP6)
  • immunocytochemistry; human; fig 3
In order to develop an array-based multiplexed analysis of signaling pathways, Invitrogen AP2A1 antibody (Affinity BioReagents, MA1-064) was used in immunocytochemistry on human samples (fig 3). Mol Cell Proteomics (2007) ncbi
mouse monoclonal (AC1-M11)
  • western blot; rat; loading ...; fig 3a
In order to find factors that interact with N-myristoylated Arf6, Invitrogen AP2A1 antibody (Affinity Bioreagents, AC1-M11) was used in western blot on rat samples (fig 3a). Methods Enzymol (2005) ncbi
mouse monoclonal (AC1-M11)
  • western blot; mouse; loading ...; fig 2c
In order to explore the effects of tensile stress on osteoblast differentiation and osteogenesis, Invitrogen AP2A1 antibody (Affinity Bioreagents, AC1-M11) was used in western blot on mouse samples (fig 2c). J Cell Physiol (2003) ncbi
Sigma-Aldrich
mouse monoclonal (100/2)
  • western blot; mouse; loading ...; fig 3a
In order to present the role of cathepsin D and its newly identified transport receptor SEZ6L2 on neurite outgrowth, Sigma-Aldrich AP2A1 antibody (Sigma, 100/2) was used in western blot on mouse samples (fig 3a). J Cell Sci (2016) ncbi
mouse monoclonal (100/2)
  • western blot; rat
Sigma-Aldrich AP2A1 antibody (Sigma, 100/2) was used in western blot on rat samples . J Comp Neurol (2007) ncbi
Articles Reviewed
  1. Richter K, Schmutz I, Darna M, Zander J, Chavan R, Albrecht U, et al. VGLUT1 Binding to Endophilin or Intersectin1 and Dynamin Phosphorylation in a Diurnal Context. Neuroscience. 2018;371:29-37 pubmed publisher
  2. Elkhatib N, Bresteau E, Baschieri F, Rioja A, van Niel G, Vassilopoulos S, et al. Tubular clathrin/AP-2 lattices pinch collagen fibers to support 3D cell migration. Science. 2017;356: pubmed publisher
  3. Poulsen E, Iannuzzi F, Rasmussen H, Maier T, Enghild J, Jørgensen A, et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci. 2017;10:59 pubmed publisher
  4. Kadlecova Z, Spielman S, Loerke D, Mohanakrishnan A, Reed D, Schmid S. Regulation of clathrin-mediated endocytosis by hierarchical allosteric activation of AP2. J Cell Biol. 2017;216:167-179 pubmed publisher
  5. Chien J, Tsen S, Chien C, Liu H, Tung C, Lin C. ?TAT1 downregulation induces mitotic catastrophe in HeLa and A549 cells. Cell Death Discov. 2016;2:16006 pubmed publisher
  6. Elkin S, Oswald N, Reed D, Mettlen M, Macmillan J, Schmid S. Ikarugamycin: A Natural Product Inhibitor of Clathrin-Mediated Endocytosis. Traffic. 2016;17:1139-49 pubmed publisher
  7. De Franceschi N, Arjonen A, Elkhatib N, Denessiouk K, Wrobel A, Wilson T, et al. Selective integrin endocytosis is driven by interactions between the integrin α-chain and AP2. Nat Struct Mol Biol. 2016;23:172-9 pubmed publisher
  8. Boonen M, Staudt C, Gilis F, Oorschot V, Klumperman J, Jadot M. Cathepsin D and its newly identified transport receptor SEZ6L2 can modulate neurite outgrowth. J Cell Sci. 2016;129:557-68 pubmed publisher
  9. Wang C, Wang Y, Hu M, Chai Z, Wu Q, Huang R, et al. Synaptotagmin-11 inhibits clathrin-mediated and bulk endocytosis. EMBO Rep. 2016;17:47-63 pubmed publisher
  10. Boucrot E, Ferreira A, Almeida Souza L, Debard S, Vallis Y, Howard G, et al. Endophilin marks and controls a clathrin-independent endocytic pathway. Nature. 2015;517:460-5 pubmed publisher
  11. Park R, Shen H, Liu L, Liu X, Ferguson S, De Camilli P. Dynamin triple knockout cells reveal off target effects of commonly used dynamin inhibitors. J Cell Sci. 2013;126:5305-12 pubmed publisher
  12. Petralia R, Yao P. AP180 and CALM in the developing hippocampus: expression at the nascent synapse and localization to trafficking organelles. J Comp Neurol. 2007;504:314-27 pubmed
  13. Zanardi A, Giorgetti L, Botrugno O, Minucci S, Milani P, Pelicci P, et al. Immunocell-array for molecular dissection of multiple signaling pathways in mammalian cells. Mol Cell Proteomics. 2007;6:939-47 pubmed
  14. Krauss M, Haucke V. Functional assay of effectors of ADP ribosylation factor 6 during clathrin/AP-2 coat recruitment to membranes. Methods Enzymol. 2005;404:388-98 pubmed
  15. Shimomura J, Ishibashi O, Ikegame M, Yoshizawa T, Ejiri S, Noda T, et al. Tensile stress induces alpha-adaptin C production in mouse calvariae in an organ culture: possible involvement of endocytosis in mechanical stress-stimulated osteoblast differentiation. J Cell Physiol. 2003;195:488-96 pubmed