This is a Validated Antibody Database (VAD) review about cow ACAN, based on 12 published articles (read how Labome selects the articles), using ACAN antibody in all methods. It is aimed to help Labome visitors find the most suited ACAN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ACAN synonym: AGC1; aggrecan core protein; CSPCP; aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122); aggrecan epidermal growth factor-like domain-1; cartilage-specific proteoglycan core protein

Invitrogen
mouse monoclonal (BC-3)
  • immunohistochemistry; horses; 1:30; loading ...; tbl 1
In order to compare 3D co-culture constructs of primary equine mesenchymal stem cells and meniscus cells for their qualification to be applied for meniscus tissue engineering, Invitrogen ACAN antibody (Affinity Bioreagents, MA3- 16888) was used in immunohistochemistry on horses samples at 1:30 (tbl 1). Tissue Eng Part A (2017) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 3
In order to determine the effects of hydroxytyrosol and procyanidin treatment on osteoarthritis using rabbits, Invitrogen ACAN antibody (Thermo Fisher Scientific, PA1-1746) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 3). Sci Rep (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - paraffin section; pig; 1:100; fig 7
  • immunohistochemistry - paraffin section; rat; 1:100; fig 7
In order to utilize a decellularized cartilage-based hydrogel to help with the compressive modulus of articular cartilage, Invitrogen ACAN antibody (ThermoFisher Scientific, MA3-16888) was used in immunohistochemistry - paraffin section on pig samples at 1:100 (fig 7) and in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 7). Acta Biomater (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - frozen section; pig; 1:100; fig 8
In order to compare devitalized and decellularized cartilage encapsulated in hydrogel pastes as chondroinduction from naturally derived cartilage matrix, Invitrogen ACAN antibody (ThermoFisher, MA3-16888) was used in immunohistochemistry - frozen section on pig samples at 1:100 (fig 8). Tissue Eng Part A (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - paraffin section; zebrafish ; fig 5
In order to analyze how Cx43-dependent skeletal phenotypes are mediated by interactions between Sema3d and HapIn1a-ECM during regeneration of fins, Invitrogen ACAN antibody (Thermo Scientific, MA3-16888) was used in immunohistochemistry - paraffin section on zebrafish samples (fig 5). PLoS ONE (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - frozen section; pig; 1:100; fig 9
  • immunohistochemistry - frozen section; rat; 1:100; fig 9
In order to research naturally derived devitalized cartilage used as chondroinductive hydrogel pastes, Invitrogen ACAN antibody (ThermoFisher, MA3-16888) was used in immunohistochemistry - frozen section on pig samples at 1:100 (fig 9) and in immunohistochemistry - frozen section on rat samples at 1:100 (fig 9). Ann Biomed Eng (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 2
In order to analyze development of experimental osteoarthritis and aggrecan degradation in murine articular cartilage by JNK-2, Invitrogen ACAN antibody (Thermo Scientific, PA1-1746) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 2). Arthritis Rheumatol (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - frozen section; zebrafish ; fig 1c
In order to analyze extracellular matrix protein expression during caudal fin regeneration in zebrafish, Invitrogen ACAN antibody (Thermo Scientific, MA3-16888) was used in immunohistochemistry - frozen section on zebrafish samples (fig 1c). Gene Expr Patterns (2015) ncbi
Abcam
mouse monoclonal (6-B-4)
  • western blot; human; 1:100; fig 5
Abcam ACAN antibody (Abcam, ab3778) was used in western blot on human samples at 1:100 (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (6-B-4)
  • immunocytochemistry; cow; 1:50; loading ...; fig 2a
Abcam ACAN antibody (Abcam, ab3778) was used in immunocytochemistry on cow samples at 1:50 (fig 2a). Nat Commun (2016) ncbi
mouse monoclonal (6-B-4)
  • immunocytochemistry; human; 1:100; loading ...; fig 3c
Abcam ACAN antibody (Abcam, ab3778) was used in immunocytochemistry on human samples at 1:100 (fig 3c). Int J Mol Med (2015) ncbi
mouse monoclonal (6-B-4)
  • immunohistochemistry; mouse
Abcam ACAN antibody (Abcam, ab3778) was used in immunohistochemistry on mouse samples . PLoS ONE (2014) ncbi
Articles Reviewed
  1. Kremer A, Ribitsch I, Reboredo J, Dürr J, Egerbacher M, Jenner F, et al. Three-Dimensional Coculture of Meniscal Cells and Mesenchymal Stem Cells in Collagen Type I Hydrogel on a Small Intestinal Matrix-A Pilot Study Toward Equine Meniscus Tissue Engineering. Tissue Eng Part A. 2017;23:390-402 pubmed publisher
  2. Mével E, Merceron C, Vinatier C, Krisa S, Richard T, Masson M, et al. Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1? activities before and after oral consumption. Sci Rep. 2016;6:33527 pubmed publisher
  3. Wu X, Liu W, Duan Z, Gao Y, Li S, Wang K, et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep. 2016;6:30563 pubmed publisher
  4. Beck E, Barragan M, Tadros M, Gehrke S, Detamore M. Approaching the compressive modulus of articular cartilage with a decellularized cartilage-based hydrogel. Acta Biomater. 2016;38:94-105 pubmed publisher
  5. Beck E, Barragan M, Libeer T, Kieweg S, Converse G, Hopkins R, et al. Chondroinduction from Naturally Derived Cartilage Matrix: A Comparison Between Devitalized and Decellularized Cartilage Encapsulated in Hydrogel Pastes. Tissue Eng Part A. 2016;22:665-79 pubmed publisher
  6. Cote A, McLeod C, Farrell M, McClanahan P, Dunagin M, Raj A, et al. Single-cell differences in matrix gene expression do not predict matrix deposition. Nat Commun. 2016;7:10865 pubmed publisher
  7. Govindan J, Tun K, Iovine M. Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration. PLoS ONE. 2016;11:e0148202 pubmed publisher
  8. Beck E, Barragan M, Tadros M, Kiyotake E, Acosta F, Kieweg S, et al. Chondroinductive Hydrogel Pastes Composed of Naturally Derived Devitalized Cartilage. Ann Biomed Eng. 2016;44:1863-80 pubmed publisher
  9. Ismail H, Miotla Zarebska J, Troeberg L, Tang X, Stott B, Yamamoto K, et al. Brief Report: JNK-2 Controls Aggrecan Degradation in Murine Articular Cartilage and the Development of Experimental Osteoarthritis. Arthritis Rheumatol. 2016;68:1165-71 pubmed publisher
  10. Govindan J, Iovine M. Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration. Gene Expr Patterns. 2015;19:21-9 pubmed publisher
  11. Ma X, Lin Y, Yang K, Yue B, Xiang H, Chen B. Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro. Int J Mol Med. 2015;36:186-94 pubmed publisher
  12. Winkler T, Mahoney E, Sinner D, Wylie C, Dahia C. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS ONE. 2014;9:e98444 pubmed publisher