This is a Validated Antibody Database (VAD) review about chicken TLN1, based on 29 published articles (read how Labome selects the articles), using TLN1 antibody in all methods. It is aimed to help Labome visitors find the most suited TLN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
TLN1 synonym: talin-1; talin-1

Knockout validation
Sigma-Aldrich
mouse monoclonal (8d4)
  • western blot knockout validation; mouse; 1:1000; fig 1
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, Sigma-Aldrich TLN1 antibody (Sigma, 8D4) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). elife (2016) ncbi
Bio-Rad
mouse monoclonal (TA205)
  • immunoprecipitation; human
Bio-Rad TLN1 antibody (Serotec, TA205) was used in immunoprecipitation on human samples . PLoS ONE (2013) ncbi
mouse monoclonal (TA205)
  • immunocytochemistry; mouse
Bio-Rad TLN1 antibody (Serotec, TA205) was used in immunocytochemistry on mouse samples . Neurobiol Dis (2013) ncbi
Sigma-Aldrich
mouse monoclonal (8d4)
  • western blot; human; 1:1000; loading ...; fig 1a
Sigma-Aldrich TLN1 antibody (Sigma, 8d4) was used in western blot on human samples at 1:1000 (fig 1a). J Cell Biol (2018) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; loading ...; fig 4f
Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on mouse samples at 1:1000 (fig 4f). Arterioscler Thromb Vasc Biol (2017) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; loading ...; fig 4k
Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, 8d4) was used in western blot on mouse samples (fig 4k). J Cell Biol (2017) ncbi
mouse monoclonal (8d4)
  • immunohistochemistry; mouse; 1:250; loading ...; fig st14
  • immunohistochemistry; chicken; 1:250; loading ...; fig st14
  • immunohistochemistry; human; 1:250; loading ...; fig st14
  • immunohistochemistry; rat; 1:250; loading ...; fig st14
In order to outline the protocols for antibodies used for immunohistochemical studies, Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, T3287) was used in immunohistochemistry on mouse samples at 1:250 (fig st14), in immunohistochemistry on chicken samples at 1:250 (fig st14), in immunohistochemistry on human samples at 1:250 (fig st14) and in immunohistochemistry on rat samples at 1:250 (fig st14). J Toxicol Pathol (2017) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; loading ...; fig s1d
In order to measure the adhesion of alphaV-class and/or alpha5beta1 integrins expressing fibroblasts initiating attachment to fibronectin, Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on mouse samples at 1:1000 (fig s1d). Nat Commun (2017) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; 1:500; fig 3b
Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in immunocytochemistry on human samples at 1:500 (fig 3b). Mol Med Rep (2016) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; loading ...; fig 1e
  • western blot; human; loading ...; fig 9a
In order to examine the role of myocardin-related transcription factor overexpression in cell migration, Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig 1e) and in western blot on human samples (fig 9a). Oncotarget (2016) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; fig 1
Sigma-Aldrich TLN1 antibody (Sigma, 8d4) was used in immunocytochemistry on human samples (fig 1). elife (2016) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; Domestic guinea pig; 1:100; fig 1
  • western blot; Domestic guinea pig; 1:1000; fig 1
Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in immunocytochemistry on Domestic guinea pig samples at 1:100 (fig 1) and in western blot on Domestic guinea pig samples at 1:1000 (fig 1). Biol Open (2016) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; fig 4
Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in immunocytochemistry on human samples (fig 4). Oncogene (2016) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; fig s4
In order to study the response to matrix rigidity by mechanical regulation of a molecular clutch that defines force transmission and transduction, Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on mouse samples at 1:1000 (fig s4). Nat Cell Biol (2016) ncbi
mouse monoclonal (8d4)
  • immunohistochemistry; human; 1:100; loading ...; fig 6d
  • western blot; human; 1:1000; loading ...; fig 6a
In order to investigate how recycled integrins assemble into new adhesions, Sigma-Aldrich TLN1 antibody (SigmaAldrich, 8d4) was used in immunohistochemistry on human samples at 1:100 (fig 6d) and in western blot on human samples at 1:1000 (fig 6a). Nat Cell Biol (2016) ncbi
mouse monoclonal (8d4)
  • western blot knockout validation; mouse; 1:1000; fig 1
In order to determine the role of Kindlin-2 tp activate integrins by cooperating with talin and induction of cell spreading by directly binding paxillin, Sigma-Aldrich TLN1 antibody (Sigma, 8D4) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). elife (2016) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; fig s1
Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig s1). Nat Commun (2015) ncbi
mouse monoclonal (8d4)
  • western blot; rat; 1:800; fig 2
In order to report that Thy-1 is a regulator of fibroblast rigidity sensing, Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, 8d4) was used in western blot on rat samples at 1:800 (fig 2). J Cell Biol (2015) ncbi
mouse monoclonal (8d4)
  • western blot; human; fig 1
Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on human samples (fig 1). PLoS ONE (2015) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; fig 4
Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in immunocytochemistry on human samples (fig 4). Nat Commun (2015) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human; 1:100
  • western blot; human; 1:500
Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, clone 8d4) was used in immunocytochemistry on human samples at 1:100 and in western blot on human samples at 1:500. Integr Biol (Camb) (2015) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; 1:1000; fig 1
In order to study phosphorylation events at sites of adhesion, Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, T3287) was used in western blot on mouse samples at 1:1000 (fig 1). Nat Commun (2015) ncbi
mouse monoclonal (8d4)
  • western blot; human
In order to examine the regulation of integrin-containing adhesion complexes by the microtubule network, Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, 8D4) was used in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human
In order to study leukocyte function-associated antigen 1 signaling with a focus on integrins, Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, 8d4) was used in immunocytochemistry on human samples . J Biol Chem (2014) ncbi
mouse monoclonal (8d4)
  • immunocytochemistry; human
  • western blot; human
Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, 8d4) was used in immunocytochemistry on human samples and in western blot on human samples . PLoS ONE (2014) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; fig 1
In order to study sorting nexin (SNX) family members interactions with beta integrins, Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig 1). J Mol Biol (2014) ncbi
mouse monoclonal (8d4)
  • western blot; mouse; fig 5, 7
In order to test if loss of Vcl and ZO-1 interactions destabilizes gap junctions in cardiomyocytes, Sigma-Aldrich TLN1 antibody (Sigma, T3287) was used in western blot on mouse samples (fig 5, 7). J Cell Sci (2014) ncbi
mouse monoclonal (8d4)
  • western blot; hamsters; 1:2,000
Sigma-Aldrich TLN1 antibody (Sigma-Aldrich, 8D4) was used in western blot on hamsters samples at 1:2,000. PLoS ONE (2013) ncbi
EMD Millipore
mouse monoclonal (TD77)
  • western blot; Domestic guinea pig; 1:500; fig 7
In order to assess the independence of secretion of beta-1 integrins in airway wall and focal adhesions proteins down regulation from airway hyperresponsiveness in asthma model, EMD Millipore TLN1 antibody (Millipore, 05-1144) was used in western blot on Domestic guinea pig samples at 1:500 (fig 7). J Cell Biochem (2016) ncbi
mouse monoclonal (TD77)
  • immunocytochemistry; human
  • western blot; human
In order to study the role of metastasis suppressor BRMS1 in cell-matrix adhesion in breast cancer cells, EMD Millipore TLN1 antibody (Millipore, 05-1144) was used in immunocytochemistry on human samples and in western blot on human samples . Mol Carcinog (2014) ncbi
Articles Reviewed
  1. Sun H, Lagarrigue F, Gingras A, Fan Z, Ley K, Ginsberg M. Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development. J Cell Biol. 2018;217:1453-1465 pubmed publisher
  2. Margraf A, Nussbaum C, Rohwedder I, Klapproth S, Kurz A, Florian A, et al. Maturation of Platelet Function During Murine Fetal Development In Vivo. Arterioscler Thromb Vasc Biol. 2017;37:1076-1086 pubmed publisher
  3. Georgiadou M, Lilja J, Jacquemet G, Guzmán C, Rafaeva M, Alibert C, et al. AMPK negatively regulates tensin-dependent integrin activity. J Cell Biol. 2017;216:1107-1121 pubmed publisher
  4. Furukawa S, Nagaike M, Ozaki K. Databases for technical aspects of immunohistochemistry. J Toxicol Pathol. 2017;30:79-107 pubmed publisher
  5. Bharadwaj M, Strohmeyer N, Colo G, Helenius J, Beerenwinkel N, Schiller H, et al. αV-class integrins exert dual roles on α5β1 integrins to strengthen adhesion to fibronectin. Nat Commun. 2017;8:14348 pubmed publisher
  6. Zeng F, Xie Y, Liao L, Li L, Chen B, Xie J, et al. Biological characterization of three immortalized esophageal epithelial cell lines. Mol Med Rep. 2016;14:4802-4810 pubmed publisher
  7. Kishi T, Mayanagi T, Iwabuchi S, Akasaka T, Sobue K. Myocardin-related transcription factor A (MRTF-A) activity-dependent cell adhesion is correlated to focal adhesion kinase (FAK) activity. Oncotarget. 2016;7:72113-72130 pubmed publisher
  8. Bouchet B, Gough R, Ammon Y, van de Willige D, Post H, Jacquemet G, et al. Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions. elife. 2016;5: pubmed publisher
  9. Roa Espitia A, Hernández Rendón E, Baltiérrez Hoyos R, Muñoz Gotera R, Cote Vélez A, Jiménez I, et al. Focal adhesion kinase is required for actin polymerization and remodeling of the cytoskeleton during sperm capacitation. Biol Open. 2016;5:1189-99 pubmed publisher
  10. Cross A, Wilson A, Guerrero M, Thomas K, Bachir A, Kubow K, et al. Breast cancer antiestrogen resistance 3-p130Cas interactions promote adhesion disassembly and invasion in breast cancer cells. Oncogene. 2016;35:5850-5859 pubmed publisher
  11. Elosegui Artola A, Oria R, Chen Y, Kosmalska A, Pérez González C, Castro N, et al. Mechanical regulation of a molecular clutch defines force transmission and transduction in response to matrix rigidity. Nat Cell Biol. 2016;18:540-8 pubmed publisher
  12. Nader G, Ezratty E, Gundersen G. FAK, talin and PIPKI? regulate endocytosed integrin activation to polarize focal adhesion assembly. Nat Cell Biol. 2016;18:491-503 pubmed publisher
  13. Álvarez Santos M, Carbajal V, Tellez Jiménez O, Martínez Cordero E, Ruiz V, Hernández Pando R, et al. Airway Hyperresponsiveness in Asthma Model Occurs Independently of Secretion of β1 Integrins in Airway Wall and Focal Adhesions Proteins Down Regulation. J Cell Biochem. 2016;117:2385-96 pubmed publisher
  14. Theodosiou M, Widmaier M, Böttcher R, Rognoni E, Veelders M, Bharadwaj M, et al. Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin. elife. 2016;5:e10130 pubmed publisher
  15. Atherton P, Stutchbury B, Wang D, Jethwa D, Tsang R, Meiler Rodriguez E, et al. Vinculin controls talin engagement with the actomyosin machinery. Nat Commun. 2015;6:10038 pubmed publisher
  16. Fiore V, Strane P, Bryksin A, White E, Hagood J, Barker T. Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction. J Cell Biol. 2015;211:173-90 pubmed publisher
  17. Roda D, Castillo J, Telechea Fernández M, Gil A, López Rodas G, Franco L, et al. EGF-Induced Acetylation of Heterogeneous Nuclear Ribonucleoproteins Is Dependent on KRAS Mutational Status in Colorectal Cancer Cells. PLoS ONE. 2015;10:e0130543 pubmed publisher
  18. Poulter N, Pollitt A, Davies A, Malinova D, Nash G, Hannon M, et al. Platelet actin nodules are podosome-like structures dependent on Wiskott-Aldrich syndrome protein and ARP2/3 complex. Nat Commun. 2015;6:7254 pubmed publisher
  19. Kiss A, Gong X, Kowalewski J, Shafqat Abbasi H, Strömblad S, Lock J. Non-monotonic cellular responses to heterogeneity in talin protein expression-level. Integr Biol (Camb). 2015;7:1171-85 pubmed publisher
  20. Robertson J, Jacquemet G, Byron A, Jones M, Warwood S, Selley J, et al. Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling. Nat Commun. 2015;6:6265 pubmed publisher
  21. Ng D, Humphries J, Byron A, Millon Frémillon A, Humphries M. Microtubule-dependent modulation of adhesion complex composition. PLoS ONE. 2014;9:e115213 pubmed publisher
  22. Uotila L, Jahan F, Soto Hinojosa L, Melandri E, Grönholm M, Gahmberg C. Specific phosphorylations transmit signals from leukocyte β2 to β1 integrins and regulate adhesion. J Biol Chem. 2014;289:32230-42 pubmed publisher
  23. Abramowski P, Ogrodowczyk C, Martin R, Pongs O. A truncation variant of the cation channel P2RX5 is upregulated during T cell activation. PLoS ONE. 2014;9:e104692 pubmed publisher
  24. Tseng H, Thorausch N, Ziegler T, Meves A, Fassler R, Böttcher R. Sorting nexin 31 binds multiple ? integrin cytoplasmic domains and regulates ?1 integrin surface levels and stability. J Mol Biol. 2014;426:3180-3194 pubmed publisher
  25. Zemljic Harpf A, Godoy J, Platoshyn O, Asfaw E, Busija A, Domenighetti A, et al. Vinculin directly binds zonula occludens-1 and is essential for stabilizing connexin-43-containing gap junctions in cardiac myocytes. J Cell Sci. 2014;127:1104-16 pubmed publisher
  26. Honda S, Shirotani Ikejima H, Tadokoro S, Tomiyama Y, Miyata T. The integrin-linked kinase-PINCH-parvin complex supports integrin ?IIb?3 activation. PLoS ONE. 2013;8:e85498 pubmed publisher
  27. Khotskaya Y, Beck B, Hurst D, Han Z, Xia W, Hung M, et al. Expression of metastasis suppressor BRMS1 in breast cancer cells results in a marked delay in cellular adhesion to matrix. Mol Carcinog. 2014;53:1011-26 pubmed publisher
  28. Zhang C, Yang C, Wang R, Jiao Y, Ampah K, Wang X, et al. c-Abl Kinase Is a Regulator of ?v?3 Integrin Mediated Melanoma A375 Cell Migration. PLoS ONE. 2013;8:e66108 pubmed publisher
  29. Koch S, Scifo E, Rokka A, Trippner P, Lindfors M, Korhonen R, et al. Cathepsin D deficiency induces cytoskeletal changes and affects cell migration pathways in the brain. Neurobiol Dis. 2013;50:107-19 pubmed publisher