This is a Validated Antibody Database (VAD) review about chicken SHH, based on 9 published articles (read how Labome selects the articles), using SHH antibody in all methods. It is aimed to help Labome visitors find the most suited SHH antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
SHH synonym: ShhNC; sonic hedgehog protein; shh unprocessed N-terminal signaling and C-terminal autoprocessing domains; sonic hedgehog homolog

Knockout validation
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; mouse; 1:20; loading ...; fig s8b
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 6b
Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on mouse samples at 1:20 (fig s8b) and in immunohistochemistry - paraffin section on mouse samples at 1:20 (fig 6b). PLoS Genet (2017) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; common quail; loading ...; fig 6c
Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on common quail samples (fig 6c). Front Cell Dev Biol (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; rat; 1:1000; loading ...; fig 5a
Abcam SHH antibody (Abcam, ab19897) was used in western blot on rat samples at 1:1000 (fig 5a). Neurochem Res (2019) ncbi
rabbit polyclonal
  • western blot; rat; 1:500
Abcam SHH antibody (Abcam, ab19897) was used in western blot on rat samples at 1:500. Int Orthop (2013) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; human
Abcam SHH antibody (Abcam, ab19897) was used in immunohistochemistry - paraffin section on human samples . J Exp Clin Cancer Res (2013) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; mouse; 1:20; loading ...; fig s8b
  • immunohistochemistry - paraffin section; mouse; 1:20; loading ...; fig 6b
Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on mouse samples at 1:20 (fig s8b) and in immunohistochemistry - paraffin section on mouse samples at 1:20 (fig 6b). PLoS Genet (2017) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry knockout validation; common quail; loading ...; fig 6c
Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in immunohistochemistry knockout validation on common quail samples (fig 6c). Front Cell Dev Biol (2016) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry; chicken; 1:200; fig s2
In order to determine regulation of neural tube closure by modulating tight junctions by cell-cycle-dependent TGFbeta-BMP antagonism, Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in immunohistochemistry on chicken samples at 1:200 (fig s2). J Cell Sci (2017) ncbi
mouse monoclonal (5E1)
  • western blot; human; 1:500; fig 4b
In order to study human carcinoma-associated mesenchymal stem cells and ovarian cancer chemotherapy through a BMP4/HH signaling loop, Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in western blot on human samples at 1:500 (fig 4b). Oncotarget (2016) ncbi
mouse monoclonal (5E1)
  • immunocytochemistry; mouse; 1:10; fig 3
Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in immunocytochemistry on mouse samples at 1:10 (fig 3). Development (2014) ncbi
mouse monoclonal (5E1)
  • immunohistochemistry - paraffin section; Gallotia galloti; 1:2
In order to study the histogenesis of the lizard visual system, Developmental Studies Hybridoma Bank SHH antibody (DSHB, 5E1) was used in immunohistochemistry - paraffin section on Gallotia galloti samples at 1:2. J Comp Neurol (2012) ncbi
Articles Reviewed
  1. Gong P, Li M, Zou C, Tian Q, Xu Z. Tissue Plasminogen Activator Causes Brain Microvascular Endothelial Cell Injury After Oxygen Glucose Deprivation by Inhibiting Sonic Hedgehog Signaling. Neurochem Res. 2019;44:441-449 pubmed publisher
  2. He H, Huang M, Sun S, Wu Y, Lin X. Epithelial heparan sulfate regulates Sonic Hedgehog signaling in lung development. PLoS Genet. 2017;13:e1006992 pubmed publisher
  3. Matsubara Y, Nakano M, Kawamura K, Tsudzuki M, Funahashi J, Agata K, et al. Inactivation of Sonic Hedgehog Signaling and Polydactyly in Limbs of Hereditary Multiple Malformation, a Novel Type of Talpid Mutant. Front Cell Dev Biol. 2016;4:149 pubmed publisher
  4. Amarnath S, Agarwala S. Cell-cycle-dependent TGF?-BMP antagonism regulates neural tube closure by modulating tight junctions. J Cell Sci. 2017;130:119-131 pubmed publisher
  5. Coffman L, Choi Y, McLean K, Allen B, di Magliano M, Buckanovich R. Human carcinoma-associated mesenchymal stem cells promote ovarian cancer chemotherapy resistance via a BMP4/HH signaling loop. Oncotarget. 2016;7:6916-32 pubmed publisher
  6. Alfaro A, Roberts B, Kwong L, Bijlsma M, Roelink H. Ptch2 mediates the Shh response in Ptch1-/- cells. Development. 2014;141:3331-9 pubmed publisher
  7. Ma X, Zhang X, Jia Y, Zu S, Han S, Xiao D, et al. Dexamethasone induces osteogenesis via regulation of hedgehog signalling molecules in rat mesenchymal stem cells. Int Orthop. 2013;37:1399-404 pubmed publisher
  8. Zhang Y, He J, Zhang F, Li H, Yue D, Wang C, et al. SMO expression level correlates with overall survival in patients with malignant pleural mesothelioma. J Exp Clin Cancer Res. 2013;32:7 pubmed publisher
  9. Romero Alemán M, Monzon Mayor M, Santos E, Lang D, Yanes C. Neuronal and glial differentiation during lizard (Gallotia galloti) visual system ontogeny. J Comp Neurol. 2012;520:2163-84 pubmed publisher