This is a Validated Antibody Database (VAD) review about chicken RAF1, based on 8 published articles (read how Labome selects the articles), using RAF1 antibody in all methods. It is aimed to help Labome visitors find the most suited RAF1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
RAF1 synonym: RAF proto-oncogene serine/threonine-protein kinase; C-RAF; MIL proto-oncogene serine/threonine-protein kinase; c-mil protein; v-raf-1 murine leukemia viral oncogene homolog 1

Knockout validation
BD Biosciences
mouse monoclonal (53/c-Raf-1)
  • western blot knockout validation; mouse; 1:500; loading ...; fig 3b
BD Biosciences RAF1 antibody (BD Biosciences, 610152) was used in western blot knockout validation on mouse samples at 1:500 (fig 3b). Nat Commun (2016) ncbi
BD Biosciences
mouse monoclonal (53/c-Raf-1)
  • western blot knockout validation; mouse; 1:500; loading ...; fig 3b
BD Biosciences RAF1 antibody (BD Biosciences, 610152) was used in western blot knockout validation on mouse samples at 1:500 (fig 3b). Nat Commun (2016) ncbi
mouse monoclonal (53/c-Raf-1)
  • immunoprecipitation; human; loading ...; fig 6b
  • western blot; human; loading ...; fig 6b
BD Biosciences RAF1 antibody (BD, 610151) was used in immunoprecipitation on human samples (fig 6b) and in western blot on human samples (fig 6b). Nat Chem Biol (2017) ncbi
mouse monoclonal (53/c-Raf-1)
  • immunoprecipitation; baker's yeast; fig 2b
  • western blot; baker's yeast; 1:5000; loading ...; fig 1h
  • immunoprecipitation; human; loading ...; fig 1l
  • western blot; human; 1:5000; loading ...; fig 1a
In order to demonstrate the bipartite role of Hsp90 in chaperoning CRAF kinase, BD Biosciences RAF1 antibody (BD Biosciences, 610151) was used in immunoprecipitation on baker's yeast samples (fig 2b), in western blot on baker's yeast samples at 1:5000 (fig 1h), in immunoprecipitation on human samples (fig 1l) and in western blot on human samples at 1:5000 (fig 1a). J Biol Chem (2016) ncbi
mouse monoclonal (53/c-Raf-1)
  • western blot; mouse; fig 5
In order to analyze regulation of NLRP3 inflammasome in adipose tissue by phosphodiesterase 3B (PDE3B), BD Biosciences RAF1 antibody (BD Biosciences, 610152) was used in western blot on mouse samples (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (53/c-Raf-1)
  • immunoprecipitation; human; tbl 3
  • western blot; human; 1:1000; tbl 2
BD Biosciences RAF1 antibody (BD, 610151) was used in immunoprecipitation on human samples (tbl 3) and in western blot on human samples at 1:1000 (tbl 2). elife (2016) ncbi
mouse monoclonal (53/c-Raf-1)
  • western blot; human
  • western blot; dog
In order to determine the contribution of protein kinase C iota to normal-polarized mammalian morphogenesis, BD Biosciences RAF1 antibody (BD Biosciences, 610151) was used in western blot on human samples and in western blot on dog samples . Carcinogenesis (2014) ncbi
mouse monoclonal (53/c-Raf-1)
  • western blot; human
BD Biosciences RAF1 antibody (BD Transduction Laboratories, 610152) was used in western blot on human samples . J Cell Sci (2013) ncbi
mouse monoclonal (53/c-Raf-1)
  • western blot; hamsters
BD Biosciences RAF1 antibody (BD Transduction Laboratories, 610152) was used in western blot on hamsters samples . Mol Cell Biol (2013) ncbi
Articles Reviewed
  1. Jerić I, Maurer G, Cavallo A, Raguz J, Desideri E, Tarkowski B, et al. A cell-autonomous tumour suppressor role of RAF1 in hepatocarcinogenesis. Nat Commun. 2016;7:13781 pubmed publisher
  2. Spencer Smith R, Koide A, Zhou Y, Eguchi R, Sha F, Gajwani P, et al. Inhibition of RAS function through targeting an allosteric regulatory site. Nat Chem Biol. 2017;13:62-68 pubmed publisher
  3. Mitra S, Ghosh B, Gayen N, Roy J, Mandal A. Bipartite Role of Heat Shock Protein 90 (Hsp90) Keeps CRAF Kinase Poised for Activation. J Biol Chem. 2016;291:24579-24593 pubmed
  4. Ahmad F, Chung Y, Tang Y, Hockman S, Liu S, Khan Y, et al. Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue. Sci Rep. 2016;6:28056 pubmed publisher
  5. Bhargava A, Pelech S, Woodard B, Kerwin J, Maherali N. Registered report: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. elife. 2016;5: pubmed publisher
  6. Linch M, Sanz Garcia M, Rosse C, Riou P, Peel N, Madsen C, et al. Regulation of polarized morphogenesis by protein kinase C iota in oncogenic epithelial spheroids. Carcinogenesis. 2014;35:396-406 pubmed publisher
  7. Barceló C, Paco N, Beckett A, Alvarez Moya B, Garrido E, Gelabert M, et al. Oncogenic K-ras segregates at spatially distinct plasma membrane signaling platforms according to its phosphorylation status. J Cell Sci. 2013;126:4553-9 pubmed publisher
  8. van der Hoeven D, Cho K, Ma X, Chigurupati S, Parton R, Hancock J. Fendiline inhibits K-Ras plasma membrane localization and blocks K-Ras signal transmission. Mol Cell Biol. 2013;33:237-51 pubmed publisher