This is a Validated Antibody Database (VAD) review about chicken NKX2-2, based on 35 published articles (read how Labome selects the articles), using NKX2-2 antibody in all methods. It is aimed to help Labome visitors find the most suited NKX2-2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NKX2-2 synonym: NKX-2.2; Nkx2.2

Developmental Studies Hybridoma Bank
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; loading ...; fig s5i
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry on mouse samples (fig s5i). Dev Cell (2022) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; human; 1:50; loading ...; fig 1b, s2a
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunocytochemistry on human samples at 1:50 (fig 1b, s2a). J Neural Transm (Vienna) (2021) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:10; loading ...; fig s2-1a
Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank, 74.5A5) was used in immunohistochemistry on mouse samples at 1:10 (fig s2-1a). elife (2020) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:5; loading ...; fig 4a
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry on mouse samples at 1:5 (fig 4a). elife (2020) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; rat; 1:200; loading ...; fig 3f
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry - frozen section on rat samples at 1:200 (fig 3f). Cell Stem Cell (2019) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; chicken; 1:100; loading ...; fig 2a
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 8s1c
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry - frozen section on chicken samples at 1:100 (fig 2a) and in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 8s1c). elife (2019) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; mouse; 1:50; loading ...; fig s1a
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunocytochemistry on mouse samples at 1:50 (fig s1a). Nat Commun (2018) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; human; 1:250; fig e1c
In order to compare the gene expression in individual cells isolated from human brain organoids, Developmental Studies Hybridoma Bank NKX2-2 antibody (DSBS, 74.5A5) was used in immunocytochemistry on human samples at 1:250 (fig e1c). Nature (2017) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; mouse; 1:100; loading ...; fig 1r
In order to identify a novel mechanism of neuro-vascular communication to control vascular development in the spinal cord, Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry - frozen section on mouse samples at 1:100 (fig 1r). Nat Commun (2017) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; mouse; 1:100; loading ...; fig 4i
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunocytochemistry on mouse samples at 1:100 (fig 4i). Nat Commun (2017) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; human; 1:50; loading ...; fig 4H
  • immunocytochemistry; African green monkey; 1:50; loading ...; fig 1G
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunocytochemistry on human samples at 1:50 (fig 4H) and in immunocytochemistry on African green monkey samples at 1:50 (fig 1G). PLoS ONE (2017) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:5; loading ...; fig 4
Developmental Studies Hybridoma Bank NKX2-2 antibody (hybridoma bank, 74.5A5) was used in immunohistochemistry on mouse samples at 1:5 (fig 4). Neural Regen Res (2016) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; mouse; 1:10; loading ...; fig 5c
In order to study regulation of Notch1 in ventral neural stem/progenitor cells of the developing spinal cord, Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry - frozen section on mouse samples at 1:10 (fig 5c). Sci Rep (2016) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - free floating section; Xenopus laevis; 1:500; loading ...; tbl 2
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry - free floating section on Xenopus laevis samples at 1:500 (tbl 2). J Comp Neurol (2017) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; fig s1
Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank, 74.5A5) was used in immunohistochemistry on mouse samples (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; human; 1:100; fig s1
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunocytochemistry on human samples at 1:100 (fig s1). Nat Neurosci (2016) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; human; 1:50; fig 1
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunocytochemistry on human samples at 1:50 (fig 1). Int J Mol Sci (2016) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; mouse; 1:50; fig st4
Developmental Studies Hybridoma Bank NKX2-2 antibody (Hybridoma Bank, 74.5A5-s) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig st4). J Clin Invest (2016) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:100; fig 1
In order to analyze regulation of formation of the columnar neural epithelium by the tumor suppressor PTEN and the PDK1 kinase, Developmental Studies Hybridoma Bank NKX2-2 antibody (Hybridoma Bank, 74.5A5) was used in immunohistochemistry on mouse samples at 1:100 (fig 1). elife (2016) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; mouse; 5 ug/ml; fig 2
Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank, 74.5A5) was used in immunohistochemistry - frozen section on mouse samples at 5 ug/ml (fig 2). Neoplasia (2016) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; chicken; 1:1000; loading ...; tbl 1
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry - frozen section on chicken samples at 1:1000 (tbl 1). PLoS ONE (2015) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; chicken; 1:80; fig 1
In order to study how signaling through the glucocorticoid receptor influences neuron survival, Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank (DSHB), 74.5A5) was used in immunohistochemistry on chicken samples at 1:80 (fig 1). Exp Neurol (2015) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; mouse; 1:200; fig s4
In order to characterize the timing of neural tube closure, embryonic viability, and neural differentiation by the required miR-302, Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5-S) was used in immunohistochemistry - frozen section on mouse samples at 1:200 (fig s4). Cell Rep (2015) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:50
In order to investigate the role of Pax6 in neuronal development, Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank developed under the auspices, 74.5A5) was used in immunohistochemistry on mouse samples at 1:50. Dev Biol (2016) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:250
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, #74.5A5-c) was used in immunohistochemistry on mouse samples at 1:250. PLoS ONE (2015) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:1000
In order to study the effect of Nkx2.2 deletion in the nervous system, Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry on mouse samples at 1:1000. Differentiation (2015) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; fig s5
In order to study Shh signaling during oncogenesis and hindbrain development due to Eya1 phosphate promotion, Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank, 74.5A5) was used in immunohistochemistry on mouse samples (fig s5). Dev Cell (2015) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:50; fig s5
In order to test if extracellular signals orient the mitotic spindle of cells in the spinal cord, Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank, 74.5A5) was used in immunohistochemistry on mouse samples at 1:50 (fig s5). Nat Commun (2015) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; fig s12
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5-s) was used in immunohistochemistry on mouse samples (fig s12). Nat Commun (2014) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; fig 3a
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry on mouse samples (fig 3a). Development (2014) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig 6j
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5a5) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig 6j). Dev Dyn (2015) ncbi
mouse monoclonal (74.5A5)
  • immunocytochemistry; mouse; 1:10; fig 3
Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 745-A5) was used in immunocytochemistry on mouse samples at 1:10 (fig 3). Development (2014) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry; mouse; 1:10
Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Hybridoma, 74.5A5-s) was used in immunohistochemistry on mouse samples at 1:10. BMC Dev Biol (2014) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - free floating section; Xenopus laevis; 1:500
Developmental Studies Hybridoma Bank NKX2-2 antibody (Developmental Studies Hybridoma Bank, 74.5A5) was used in immunohistochemistry - free floating section on Xenopus laevis samples at 1:500. J Comp Neurol (2014) ncbi
mouse monoclonal (74.5A5)
  • immunohistochemistry - paraffin section; mouse; 1:25
In order to study the roles of GATA4 and GATA6 in mouse pancreas organogenesis, Developmental Studies Hybridoma Bank NKX2-2 antibody (DSHB, 74.5A5) was used in immunohistochemistry - paraffin section on mouse samples at 1:25. J Clin Invest (2012) ncbi
Articles Reviewed
  1. Miguel Escalada I, Maestro M, Balboa D, Elek A, Bernal A, Bernardo E, et al. Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster. Dev Cell. 2022;57:1922-1936.e9 pubmed publisher
  2. Jansch C, Ziegler G, Forero A, Gredy S, W xe4 ldchen S, Vitale M, et al. Serotonin-specific neurons differentiated from human iPSCs form distinct subtypes with synaptic protein assembly. J Neural Transm (Vienna). 2021;128:225-241 pubmed publisher
  3. Yoshida S, Aoki K, Fujiwara K, Nakakura T, Kawamura A, Yamada K, et al. The novel ciliogenesis regulator DYRK2 governs Hedgehog signaling during mouse embryogenesis. elife. 2020;9: pubmed publisher
  4. Gigante E, Taylor M, Ivanova A, Kahn R, Caspary T. ARL13B regulates Sonic hedgehog signaling from outside primary cilia. elife. 2020;9: pubmed publisher
  5. Neumann B, Baror R, Zhao C, SEGEL M, Dietmann S, Rawji K, et al. Metformin Restores CNS Remyelination Capacity by Rejuvenating Aged Stem Cells. Cell Stem Cell. 2019;25:473-485.e8 pubmed publisher
  6. Nam H, Jeon S, An H, Yoo J, Lee H, Lee S, et al. Critical roles of ARHGAP36 as a signal transduction mediator of Shh pathway in lateral motor columnar specification. elife. 2019;8: pubmed publisher
  7. Luisier R, Tyzack G, Hall C, Mitchell J, Devine H, Taha D, et al. Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS. Nat Commun. 2018;9:2010 pubmed publisher
  8. Quadrato G, Nguyen T, Macosko E, Sherwood J, Min Yang S, Berger D, et al. Cell diversity and network dynamics in photosensitive human brain organoids. Nature. 2017;545:48-53 pubmed publisher
  9. Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti H, et al. Motor neurons control blood vessel patterning in the developing spinal cord. Nat Commun. 2017;8:14583 pubmed publisher
  10. Fujii K, Shi Z, Zhulyn O, Denans N, Barna M. Pervasive translational regulation of the cell signalling circuitry underlies mammalian development. Nat Commun. 2017;8:14443 pubmed publisher
  11. Yamashita T, Miyamoto Y, Bando Y, Ono T, Kobayashi S, Doi A, et al. Differentiation of oligodendrocyte progenitor cells from dissociated monolayer and feeder-free cultured pluripotent stem cells. PLoS ONE. 2017;12:e0171947 pubmed publisher
  12. Weng C, Ding M, Chang L, Ren M, Zhang H, Lu Z, et al. Ankfy1 is dispensable for neural stem/precursor cell development. Neural Regen Res. 2016;11:1804-1809 pubmed publisher
  13. Li Y, Tzatzalos E, Kwan K, Grumet M, Cai L. Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development. Sci Rep. 2016;6:38665 pubmed publisher
  14. Morona R, Ferran J, Puelles L, González A. Gene expression analysis of developing cell groups in the pretectal region of Xenopus laevis. J Comp Neurol. 2017;525:715-752 pubmed publisher
  15. Airik R, Schueler M, Airik M, Cho J, Ulanowicz K, Porath J, et al. SDCCAG8 Interacts with RAB Effector Proteins RABEP2 and ERC1 and Is Required for Hedgehog Signaling. PLoS ONE. 2016;11:e0156081 pubmed publisher
  16. Xue Y, Qian H, Hu J, Zhou B, Zhou Y, Hu X, et al. Sequential regulatory loops as key gatekeepers for neuronal reprogramming in human cells. Nat Neurosci. 2016;19:807-15 pubmed publisher
  17. Douvaras P, Rusielewicz T, Kim K, Haines J, CASACCIA P, Fossati V. Epigenetic Modulation of Human Induced Pluripotent Stem Cell Differentiation to Oligodendrocytes. Int J Mol Sci. 2016;17: pubmed publisher
  18. Dooves S, Bugiani M, Postma N, Polder E, Land N, Horan S, et al. Astrocytes are central in the pathomechanisms of vanishing white matter. J Clin Invest. 2016;126:1512-24 pubmed publisher
  19. Grego Bessa J, Bloomekatz J, Castel P, Omelchenko T, Baselga J, Anderson K. The tumor suppressor PTEN and the PDK1 kinase regulate formation of the columnar neural epithelium. elife. 2016;5:e12034 pubmed publisher
  20. Misuraca K, Hu G, Barton K, Chung A, Becher O. A Novel Mouse Model of Diffuse Intrinsic Pontine Glioma Initiated in Pax3-Expressing Cells. Neoplasia. 2016;18:60-70 pubmed publisher
  21. Pirson M, Debrulle S, Clippe A, Clotman F, Knoops B. Thioredoxin-2 Modulates Neuronal Programmed Cell Death in the Embryonic Chick Spinal Cord in Basal and Target-Deprived Conditions. PLoS ONE. 2015;10:e0142280 pubmed publisher
  22. Gallina D, Zelinka C, Cebulla C, Fischer A. Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity. Exp Neurol. 2015;273:114-25 pubmed publisher
  23. Parchem R, Moore N, Fish J, Parchem J, Braga T, Shenoy A, et al. miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability. Cell Rep. 2015;12:760-73 pubmed publisher
  24. Huettl R, Eckstein S, Stahl T, Petricca S, Ninkovic J, Götz M, et al. Functional dissection of the Pax6 paired domain: Roles in neural tube patterning and peripheral nervous system development. Dev Biol. 2016;413:86-103 pubmed publisher
  25. Wende C, Zoubaa S, Blak A, Echevarria D, Martinez S, Guillemot F, et al. Hairy/Enhancer-of-Split MEGANE and Proneural MASH1 Factors Cooperate Synergistically in Midbrain GABAergic Neurogenesis. PLoS ONE. 2015;10:e0127681 pubmed publisher
  26. Jarrar W, Vauti F, Arnold H, Holz A. Generation of a Nkx2.2(Cre) knock-in mouse line: Analysis of cell lineages in the central nervous system. Differentiation. 2015;89:70-6 pubmed publisher
  27. Eisner A, Pazyra Murphy M, Durresi E, Zhou P, Zhao X, Chadwick E, et al. The Eya1 phosphatase promotes Shh signaling during hindbrain development and oncogenesis. Dev Cell. 2015;33:22-35 pubmed publisher
  28. Arbeille E, Reynaud F, Sanyas I, Bozon M, Kindbeiter K, Causeret F, et al. Cerebrospinal fluid-derived Semaphorin3B orients neuroepithelial cell divisions in the apicobasal axis. Nat Commun. 2015;6:6366 pubmed publisher
  29. Shi X, Zhang Z, Zhan X, Cao M, Satoh T, Akira S, et al. An epigenetic switch induced by Shh signalling regulates gene activation during development and medulloblastoma growth. Nat Commun. 2014;5:5425 pubmed publisher
  30. Goggolidou P, Stevens J, Agueci F, Keynton J, Wheway G, Grimes D, et al. ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis. Development. 2014;141:3966-77 pubmed publisher
  31. Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher
  32. Alfaro A, Roberts B, Kwong L, Bijlsma M, Roelink H. Ptch2 mediates the Shh response in Ptch1-/- cells. Development. 2014;141:3331-9 pubmed publisher
  33. Tiwari S, Dharmarajan S, Shivanna M, Otteson D, Belecky Adams T. Histone deacetylase expression patterns in developing murine optic nerve. BMC Dev Biol. 2014;14:30 pubmed publisher
  34. Dominguez L, González A, Moreno N. Characterization of the hypothalamus of Xenopus laevis during development. II. The basal regions. J Comp Neurol. 2014;522:1102-31 pubmed publisher
  35. Carrasco M, Delgado I, Soria B, Martin F, Rojas A. GATA4 and GATA6 control mouse pancreas organogenesis. J Clin Invest. 2012;122:3504-15 pubmed publisher