This is a Validated Antibody Database (VAD) review about chicken NEFM, based on 25 published articles (read how Labome selects the articles), using NEFM antibody in all methods. It is aimed to help Labome visitors find the most suited NEFM antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NEFM synonym: NEF3; neurofilament medium polypeptide; 160 kDa neurofilament protein; NF-M c-terminus; NF-M protein; neurofilament 3 (150kDa medium); neurofilament triplet M protein; neurofilament, medium polypeptide 150kDa

Invitrogen
mouse monoclonal (RMO 14.9)
  • immunohistochemistry - frozen section; pig; 1:750; loading ...; fig 3a
In order to characterize gyrencephalic swine brains exposed to true open field blasts, Invitrogen NEFM antibody (Invitrogen, 34-1000) was used in immunohistochemistry - frozen section on pig samples at 1:750 (fig 3a). PLoS ONE (2017) ncbi
mouse monoclonal (RMO 14.9)
  • immunohistochemistry - paraffin section; mouse; 1:2000; fig 5
In order to study the cerebellar cortex of the mouse for the temporal sequence of autolysis, Invitrogen NEFM antibody (Invitrogen, 34-1000) was used in immunohistochemistry - paraffin section on mouse samples at 1:2000 (fig 5). J Comp Pathol (2016) ncbi
mouse monoclonal (RMO 14.9)
  • immunohistochemistry; rat; 1 ug/ml
In order to perform a time course to study impaired axoplasmic transport and neurofilament compaction, Invitrogen NEFM antibody (Zymed, 34-1000) was used in immunohistochemistry on rat samples at 1 ug/ml. Brain Res (2012) ncbi
mouse monoclonal (RMO 14.9)
  • immunohistochemistry - paraffin section; sheep; fig 3
In order to describe the clinicopathological changes in the second occurrence of neuroaxonal dystrophy reported in Merino lambs, Invitrogen NEFM antibody (Invitrogen, clone RMO 14.9) was used in immunohistochemistry - paraffin section on sheep samples (fig 3). J Comp Pathol (2012) ncbi
mouse monoclonal (RMO 14.9)
  • western blot; mouse; 1:1000; fig 2
In order to treat RGC-5 cells with known differentiating agents and determine which RGC or neuronal markers are expressed, Invitrogen NEFM antibody (Zymed, 34-1000) was used in western blot on mouse samples at 1:1000 (fig 2). Invest Ophthalmol Vis Sci (2010) ncbi
mouse monoclonal (RMO 14.9)
  • immunohistochemistry - paraffin section; rat; 1:500
In order to develop a new weight-drop head-injury apparatus and assess the treated rats, Invitrogen NEFM antibody (Zymed, 34-1000) was used in immunohistochemistry - paraffin section on rat samples at 1:500. J Neurosci Methods (2006) ncbi
mouse monoclonal (RMO 14.9)
  • immunohistochemistry - frozen section; mouse; 1:200
  • immunocytochemistry; mouse; 1:200
In order to examine the differentiation of embryonic stem cells after their transplantation into the eyes of mice with hereditary retinal degeneration, Invitrogen NEFM antibody (Zymed, 34-1000) was used in immunohistochemistry - frozen section on mouse samples at 1:200 and in immunocytochemistry on mouse samples at 1:200. Brain Res (2004) ncbi
Enzo Life Sciences
mouse monoclonal (3H11)
  • other; rat; loading ...; fig 5
  • immunohistochemistry; human; loading ...; fig 2c
Enzo Life Sciences NEFM antibody (Enzo Life Sciences, ADI-NBA-140-E) was used in other on rat samples (fig 5) and in immunohistochemistry on human samples (fig 2c). PLoS ONE (2015) ncbi
EMD Millipore
rabbit polyclonal
  • immunocytochemistry; rat; loading ...; fig 7
In order to generate an atoxic derivative of Botulism neurotoxin/C1 and assess whether it retains neuron-specific targeting without concomitant toxic host responses, EMD Millipore NEFM antibody (Millipore, ab1987) was used in immunocytochemistry on rat samples (fig 7). Sci Rep (2017) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; 1:200; fig 2c
  • western blot; mouse; loading ...; fig 5f
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry on mouse samples at 1:200 (fig 2c) and in western blot on mouse samples (fig 5f). Nat Commun (2017) ncbi
mouse monoclonal (NN18)
  • immunocytochemistry; mouse; 1:500; fig 6g
  • immunohistochemistry; mouse; 1:500; loading ...; fig 3a
In order to develop a novel method for obtaining perivascular-resident macrophage-like melanocytes, pericytes, and endothelial cells primary cells to study the vestibular blood-labyrinth barrier, EMD Millipore NEFM antibody (EMDmillipore, MAB5254) was used in immunocytochemistry on mouse samples at 1:500 (fig 6g) and in immunohistochemistry on mouse samples at 1:500 (fig 3a). Hear Res (2017) ncbi
rabbit polyclonal
  • immunocytochemistry; mouse; loading ...; fig 2a
  • western blot; mouse; loading ...; fig 2d
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunocytochemistry on mouse samples (fig 2a) and in western blot on mouse samples (fig 2d). J Cell Biol (2017) ncbi
mouse monoclonal (NN18)
  • immunohistochemistry - frozen section; rat; 1:200; loading ...; fig 6
In order to investigate 2-methylcitrate-induced brain toxicity, EMD Millipore NEFM antibody (Millipore, MAB5254) was used in immunohistochemistry - frozen section on rat samples at 1:200 (fig 6). Mol Genet Metab (2016) ncbi
mouse monoclonal (NN18)
  • immunohistochemistry - paraffin section; human; fig 2
EMD Millipore NEFM antibody (millipore, MAB5254) was used in immunohistochemistry - paraffin section on human samples (fig 2). PLoS ONE (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse; 1:1000; fig s3
In order to learn about induction of selective motor neuron degeneration through toxic gain of function due to ALS-associated mutant FUS, EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig s3). Nat Commun (2016) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - frozen section on mouse samples . Science (2015) ncbi
rabbit polyclonal
  • immunohistochemistry; human; 1:200; loading ...; fig s1d
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry on human samples at 1:200 (fig s1d). Stem Cells (2016) ncbi
mouse monoclonal (NN18)
  • immunohistochemistry; pig; 1:5000
EMD Millipore NEFM antibody (Millipore, MAB5254) was used in immunohistochemistry on pig samples at 1:5000. Ann Neurol (2015) ncbi
rabbit polyclonal
  • western blot; mouse; fig 6
EMD Millipore NEFM antibody (EMD Millipore, AB1987) was used in western blot on mouse samples (fig 6). Nat Neurosci (2014) ncbi
rabbit polyclonal
  • western blot; mouse; fig s4
In order to elucidate the molecular mechanisms underlying paranodal junction assembly, EMD Millipore NEFM antibody (Millipore, AB1987) was used in western blot on mouse samples (fig s4). Nat Neurosci (2014) ncbi
rabbit polyclonal
  • immunohistochemistry; mouse; fig 5
EMD Millipore NEFM antibody (Chemicon, AB1987) was used in immunohistochemistry on mouse samples (fig 5). Biol Open (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse
EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - paraffin section on mouse samples . J Neurosci (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; chicken
In order to localize and characterize cholesterol-rich membrane microdomains in the inner ear, EMD Millipore NEFM antibody (Chemicon, AB1987) was used in immunohistochemistry - frozen section on chicken samples . J Proteomics (2014) ncbi
rabbit polyclonal
  • immunohistochemistry - frozen section; mouse
In order to study the role of neuregulin-1/glial growth factor in Schwann cell migration and its mechanism, EMD Millipore NEFM antibody (Millipore, AB1987) was used in immunohistochemistry - frozen section on mouse samples . Genes Cells (2014) ncbi
Developmental Studies Hybridoma Bank
mouse monoclonal (4H6)
  • immunohistochemistry; chicken; 1:10; loading ...; fig 4h
In order to elucidate the influence of inductive interactions between epithelium and mesenchyme on enteric nervous system development, Developmental Studies Hybridoma Bank NEFM antibody (DSHB, 4H6) was used in immunohistochemistry on chicken samples at 1:10 (fig 4h). Development (2016) ncbi
Articles Reviewed
  1. Vazquez Cintron E, Beske P, Tenezaca L, Tran B, Oyler J, Glotfelty E, et al. Engineering Botulinum Neurotoxin C1 as a Molecular Vehicle for Intra-Neuronal Drug Delivery. Sci Rep. 2017;7:42923 pubmed publisher
  2. Brügger V, Duman M, Bochud M, Münger E, Heller M, Ruff S, et al. Delaying histone deacetylase response to injury accelerates conversion into repair Schwann cells and nerve regeneration. Nat Commun. 2017;8:14272 pubmed publisher
  3. Kallakuri S, Desai A, Feng K, Tummala S, Saif T, Chen C, et al. Neuronal Injury and Glial Changes Are Hallmarks of Open Field Blast Exposure in Swine Frontal Lobe. PLoS ONE. 2017;12:e0169239 pubmed publisher
  4. Zhang J, Chen S, Cai J, Hou Z, Wang X, Kachelmeier A, et al. Culture media-based selection of endothelial cells, pericytes, and perivascular-resident macrophage-like melanocytes from the young mouse vestibular system. Hear Res. 2017;345:10-22 pubmed publisher
  5. Wakatsuki S, Tokunaga S, Shibata M, Araki T. GSK3B-mediated phosphorylation of MCL1 regulates axonal autophagy to promote Wallerian degeneration. J Cell Biol. 2017;216:477-493 pubmed publisher
  6. Cudré Cung H, Zavadakova P, Do Vale Pereira S, Remacle N, Henry H, Ivanisevic J, et al. Ammonium accumulation is a primary effect of 2-methylcitrate exposure in an in vitro model for brain damage in methylmalonic aciduria. Mol Genet Metab. 2016;119:57-67 pubmed publisher
  7. Wharton K, Quigley C, Themeles M, Dunstan R, Doyle K, Cahir McFarland E, et al. JC Polyomavirus Abundance and Distribution in Progressive Multifocal Leukoencephalopathy (PML) Brain Tissue Implicates Myelin Sheath in Intracerebral Dissemination of Infection. PLoS ONE. 2016;11:e0155897 pubmed publisher
  8. Finnie J, Blumbergs P, Manavis J. Temporal Sequence of Autolysis in the Cerebellar Cortex of the Mouse. J Comp Pathol. 2016;154:323-8 pubmed publisher
  9. Sharma A, Lyashchenko A, Lu L, Nasrabady S, Elmaleh M, Mendelsohn M, et al. ALS-associated mutant FUS induces selective motor neuron degeneration through toxic gain of function. Nat Commun. 2016;7:10465 pubmed publisher
  10. Amin N, Bai G, Klug J, Bonanomi D, Pankratz M, Gifford W, et al. Loss of motoneuron-specific microRNA-218 causes systemic neuromuscular failure. Science. 2015;350:1525-9 pubmed publisher
  11. Nagy N, Barad C, Graham H, Hotta R, Cheng L, Fejszak N, et al. Sonic hedgehog controls enteric nervous system development by patterning the extracellular matrix. Development. 2016;143:264-75 pubmed publisher
  12. Uchida H, Morita T, Niizuma K, Kushida Y, Kuroda Y, Wakao S, et al. Transplantation of Unique Subpopulation of Fibroblasts, Muse Cells, Ameliorates Experimental Stroke Possibly via Robust Neuronal Differentiation. Stem Cells. 2016;34:160-73 pubmed publisher
  13. Fournier A, Hogan J, Rajbhandari L, Shrestha S, Venkatesan A, Ramesh K. Changes in Neurofilament and Microtubule Distribution following Focal Axon Compression. PLoS ONE. 2015;10:e0131617 pubmed publisher
  14. Duque S, Arnold W, Odermatt P, Li X, Porensky P, Schmelzer L, et al. A large animal model of spinal muscular atrophy and correction of phenotype. Ann Neurol. 2015;77:399-414 pubmed publisher
  15. Ho T, Zollinger D, Chang K, Xu M, Cooper E, Stankewich M, et al. A hierarchy of ankyrin-spectrin complexes clusters sodium channels at nodes of Ranvier. Nat Neurosci. 2014;17:1664-72 pubmed publisher
  16. Chang K, Zollinger D, Susuki K, Sherman D, Makara M, Brophy P, et al. Glial ankyrins facilitate paranodal axoglial junction assembly. Nat Neurosci. 2014;17:1673-81 pubmed publisher
  17. Liu C, Lin C, Gao C, May Simera H, Swaroop A, Li T. Null and hypomorph Prickle1 alleles in mice phenocopy human Robinow syndrome and disrupt signaling downstream of Wnt5a. Biol Open. 2014;3:861-70 pubmed publisher
  18. Bull S, Bin J, Beaumont E, Boutet A, Krimpenfort P, Sadikot A, et al. Progressive disorganization of paranodal junctions and compact myelin due to loss of DCC expression by oligodendrocytes. J Neurosci. 2014;34:9768-78 pubmed publisher
  19. Thomas P, Cheng A, Colby C, Liu L, Patel C, Josephs L, et al. Localization and proteomic characterization of cholesterol-rich membrane microdomains in the inner ear. J Proteomics. 2014;103:178-93 pubmed publisher
  20. Wakatsuki S, Araki T, Sehara Fujisawa A. Neuregulin-1/glial growth factor stimulates Schwann cell migration by inducing ?5 ?1 integrin-ErbB2-focal adhesion kinase complex formation. Genes Cells. 2014;19:66-77 pubmed publisher
  21. Zakaria N, Kallakuri S, Bandaru S, Cavanaugh J. Temporal assessment of traumatic axonal injury in the rat corpus callosum and optic chiasm. Brain Res. 2012;1467:81-90 pubmed publisher
  22. Kessell A, Finnie J, Blumbergs P, Manavis J, Jerrett I. Neuroaxonal dystrophy in Australian Merino lambs. J Comp Pathol. 2012;147:62-72 pubmed publisher
  23. Wood J, Chidlow G, Tran T, Crowston J, Casson R. A comparison of differentiation protocols for RGC-5 cells. Invest Ophthalmol Vis Sci. 2010;51:3774-83 pubmed publisher
  24. Pal J, Toth Z, Farkas O, Kellenyi L, Doczi T, Gallyas F. Selective induction of ultrastructural (neurofilament) compaction in axons by means of a new head-injury apparatus. J Neurosci Methods. 2006;153:283-9 pubmed
  25. Meyer J, Katz M, Maruniak J, Kirk M. Neural differentiation of mouse embryonic stem cells in vitro and after transplantation into eyes of mutant mice with rapid retinal degeneration. Brain Res. 2004;1014:131-44 pubmed