This is a Validated Antibody Database (VAD) review about chicken NEFL, based on 6 published articles (read how Labome selects the articles), using NEFL antibody in all methods. It is aimed to help Labome visitors find the most suited NEFL antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
NEFL synonym: neurofilament light polypeptide; neurofilament, light polypeptide 68kDa

Knockout validation
Sigma-Aldrich
mouse monoclonal (NR4)
  • western blot knockout validation; mouse; loading ...; fig 1b
Sigma-Aldrich NEFL antibody (Sigma-Aldrich, N5139) was used in western blot knockout validation on mouse samples (fig 1b). Neurosci Lett (2017) ncbi
Sigma-Aldrich
mouse monoclonal (NR4)
  • western blot knockout validation; mouse; loading ...; fig 1b
Sigma-Aldrich NEFL antibody (Sigma-Aldrich, N5139) was used in western blot knockout validation on mouse samples (fig 1b). Neurosci Lett (2017) ncbi
mouse monoclonal (NR4)
  • immunohistochemistry; rat; 1:1000; loading ...; tbl 2
  • western blot; rat; 1:1000; loading ...; tbl 2
In order to test if interleukin-1b derived from microglia affects myelination and axon development in lipopolysaccharide-treated cells, Sigma-Aldrich NEFL antibody (Sigma, N5139) was used in immunohistochemistry on rat samples at 1:1000 (tbl 2) and in western blot on rat samples at 1:1000 (tbl 2). J Neuroinflammation (2017) ncbi
mouse monoclonal (NR4)
  • western blot; human; 1:500; fig ev3
Sigma-Aldrich NEFL antibody (Sigma, 5139) was used in western blot on human samples at 1:500 (fig ev3). EMBO Mol Med (2015) ncbi
mouse monoclonal (NR4)
  • immunohistochemistry; mouse
  • western blot; mouse
Sigma-Aldrich NEFL antibody (Sigma, NR-4) was used in immunohistochemistry on mouse samples and in western blot on mouse samples . J Neurosci (2014) ncbi
mouse monoclonal (NR4)
  • immunohistochemistry - frozen section; rat
  • immunohistochemistry - frozen section; mouse; 1:400
In order to study the properties of the intrnsically photo-sensitive retinal ganglion cells of a diurnal rodent species, Sigma-Aldrich NEFL antibody (Sigma-Aldrich, N5139) was used in immunohistochemistry - frozen section on rat samples and in immunohistochemistry - frozen section on mouse samples at 1:400. PLoS ONE (2013) ncbi
mouse monoclonal (NR4)
  • immunohistochemistry - frozen section; mouse; 1:400
In order to study the heterogeneity of intrinsically photosensitive retinal ganglion cells in the mouse, Sigma-Aldrich NEFL antibody (Sigma-Aldrich, N5139) was used in immunohistochemistry - frozen section on mouse samples at 1:400. J Comp Neurol (2013) ncbi
Articles Reviewed
  1. Rutherford N, Brooks M, Riffe C, Gorion K, Howard J, Dhillon J, et al. Prion-like transmission of ?-synuclein pathology in the context of an NFL null background. Neurosci Lett. 2017;661:114-120 pubmed publisher
  2. Han Q, Lin Q, Huang P, Chen M, Hu X, Fu H, et al. Microglia-derived IL-1? contributes to axon development disorders and synaptic deficit through p38-MAPK signal pathway in septic neonatal rats. J Neuroinflammation. 2017;14:52 pubmed publisher
  3. Fernández Santiago R, Carballo Carbajal I, Castellano G, Torrent R, Richaud Y, Sánchez Danés A, et al. Aberrant epigenome in iPSC-derived dopaminergic neurons from Parkinson's disease patients. EMBO Mol Med. 2015;7:1529-46 pubmed publisher
  4. Rao M, McBrayer M, Campbell J, Kumar A, Hashim A, Sershen H, et al. Specific calpain inhibition by calpastatin prevents tauopathy and neurodegeneration and restores normal lifespan in tau P301L mice. J Neurosci. 2014;34:9222-34 pubmed publisher
  5. Karnas D, Hicks D, Mordel J, Pevet P, Meissl H. Intrinsic photosensitive retinal ganglion cells in the diurnal rodent, Arvicanthis ansorgei. PLoS ONE. 2013;8:e73343 pubmed publisher
  6. Karnas D, Mordel J, Bonnet D, Pevet P, Hicks D, Meissl H. Heterogeneity of intrinsically photosensitive retinal ganglion cells in the mouse revealed by molecular phenotyping. J Comp Neurol. 2013;521:912-32 pubmed publisher