This is a Validated Antibody Database (VAD) review about chicken MAPT, based on 17 published articles (read how Labome selects the articles), using MAPT antibody in all methods. It is aimed to help Labome visitors find the most suited MAPT antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Invitrogen
mouse monoclonal (T46)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1b
In order to show that the tau family members MAP2 and MAP4 are subject to reversible acetylation, Invitrogen MAPT antibody (Thermofisher, T46) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1b). PLoS ONE (2016) ncbi
Invitrogen
mouse monoclonal (T46)
  • western blot; fruit fly ; 1:1000; loading ...; fig 3g
Invitrogen MAPT antibody (Invitrogen, 13-6400) was used in western blot on fruit fly samples at 1:1000 (fig 3g). Nat Commun (2021) ncbi
mouse monoclonal (T46)
  • western blot; mouse; loading ...; fig 4a
Invitrogen MAPT antibody (Thermo Fisher Scientific, 13-6400) was used in western blot on mouse samples (fig 4a). Cell (2021) ncbi
mouse monoclonal (T46)
  • immunoprecipitation; human
  • western blot; human; 1:1000; fig 1b
In order to study the mechanisms of pathogenesis via tau acetylation in Alzheimer's disease, Invitrogen MAPT antibody (Thermo Fisher, 13-6400) was used in immunoprecipitation on human samples and in western blot on human samples at 1:1000 (fig 1b). Sci Rep (2017) ncbi
mouse monoclonal (T46)
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1b
In order to show that the tau family members MAP2 and MAP4 are subject to reversible acetylation, Invitrogen MAPT antibody (Thermofisher, T46) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1b). PLoS ONE (2016) ncbi
mouse monoclonal (T46)
  • immunocytochemistry; rat; 1:100; loading ...; fig 4b
In order to report the effects of okadaic acid-induced tau hyperphosphorylation on secreted and cellular levels of brain-derived neurotrophic factor in primary cortical neurons, Invitrogen MAPT antibody (Thermofisher, 13-6400) was used in immunocytochemistry on rat samples at 1:100 (fig 4b). J Chem Neuroanat (2017) ncbi
mouse monoclonal (T46)
  • immunocytochemistry; human; 1:200; fig 2c
  • western blot; human; 1:1000; fig 2e
In order to discuss the contribution of leucine-rich repeat kinase 2 mutations to Parkinson's disease, Invitrogen MAPT antibody (Invitrogen, 136400) was used in immunocytochemistry on human samples at 1:200 (fig 2c) and in western blot on human samples at 1:1000 (fig 2e). J Neuroinflammation (2016) ncbi
mouse monoclonal (T46)
  • western blot; mouse; 1:1000; fig 7
In order to assess rescue of Alzheimer's disease in the mouse by improved proteostasis in the secretory pathway, Invitrogen MAPT antibody (Invitrogen, T46) was used in western blot on mouse samples at 1:1000 (fig 7). Brain (2016) ncbi
mouse monoclonal (T46)
  • immunohistochemistry; human; 1:500; fig 6
  • western blot; human; fig 2
In order to assess using the biochemical properties of tau to classify tauopathies, Invitrogen MAPT antibody (Thermo Scientific, T46) was used in immunohistochemistry on human samples at 1:500 (fig 6) and in western blot on human samples (fig 2). Acta Neuropathol (2016) ncbi
mouse monoclonal (T46)
  • western blot; human
In order to identify small-molecules that inhibit the interaction between CDK5 and p25, Invitrogen MAPT antibody (Invitrogen, 13-6400) was used in western blot on human samples . Chem Biol (2015) ncbi
mouse monoclonal (T46)
  • western blot; human; 1:1000
In order to report a case of glial mixed tau and TDP-43 proteinopathies in a Japanese patient, Invitrogen MAPT antibody (Zymed, T46) was used in western blot on human samples at 1:1000. Brain Pathol (2016) ncbi
mouse monoclonal (T46)
  • western blot; human; fig 1
In order to characterize two mouse models overexpressing different human tau protein constructs, Invitrogen MAPT antibody (Invitrogen, 13-6400) was used in western blot on human samples (fig 1). Cell Mol Life Sci (2015) ncbi
MilliporeSigma
domestic rabbit polyclonal
  • immunocytochemistry; human; loading ...; fig 3l
MilliporeSigma MAPT antibody (Sigma-Aldrich, T6402) was used in immunocytochemistry on human samples (fig 3l). Nat Med (2018) ncbi
domestic rabbit polyclonal
  • western blot; mouse; 1:1000; loading ...; fig 2c
In order to investigate the expression patterns of HA synthases in the murine central nervous system, MilliporeSigma MAPT antibody (Sigma, T6402) was used in western blot on mouse samples at 1:1000 (fig 2c). J Alzheimers Dis (2017) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; fig 7
MilliporeSigma MAPT antibody (Sigma, T-6402) was used in immunocytochemistry on mouse samples (fig 7). Acta Neuropathol (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry; rat; 1:750; loading ...; fig 3e
In order to use electroconducting microfibers to synergistically stimulate the proliferation and migration of glial progenitor cells, MilliporeSigma MAPT antibody (Sigma, T-6402) was used in immunohistochemistry on rat samples at 1:750 (fig 3e). Acta Biomater (2016) ncbi
mouse monoclonal (Tau-2)
  • western blot; mouse; 1:1000; fig 3
In order to analyze a mouse model where human neural stem cells alleviate Alzheimer-like pathology, MilliporeSigma MAPT antibody (Sigma-Aldrich, T5530) was used in western blot on mouse samples at 1:1000 (fig 3). Mol Neurodegener (2015) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; human; 1:100
MilliporeSigma MAPT antibody (Sigma-Aldrich, T6402) was used in immunocytochemistry on human samples at 1:100. Methods Mol Biol (2016) ncbi
Articles Reviewed
  1. Subramanian M, Hyeon S, Das T, Suh Y, Kim Y, Lee J, et al. UBE4B, a microRNA-9 target gene, promotes autophagy-mediated Tau degradation. Nat Commun. 2021;12:3291 pubmed publisher
  2. Shin M, Vázquez Rosa E, Koh Y, Dhar M, Chaubey K, Cintrón Pérez C, et al. Reducing acetylated tau is neuroprotective in brain injury. Cell. 2021;184:2715-2732.e23 pubmed publisher
  3. Wang C, Najm R, Xu Q, Jeong D, Walker D, Balestra M, et al. Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector. Nat Med. 2018;24:647-657 pubmed publisher
  4. Trzeciakiewicz H, Tseng J, Wander C, Madden V, Tripathy A, Yuan C, et al. A Dual Pathogenic Mechanism Links Tau Acetylation to Sporadic Tauopathy. Sci Rep. 2017;7:44102 pubmed publisher
  5. Li Y, Li Z, Jin T, Wang Z, Zhao P. Tau Pathology Promotes the Reorganization of the Extracellular Matrix and Inhibits the Formation of Perineuronal Nets by Regulating the Expression and the Distribution of Hyaluronic Acid Synthases. J Alzheimers Dis. 2017;57:395-409 pubmed publisher
  6. Hwang A, Trzeciakiewicz H, Friedmann D, Yuan C, Marmorstein R, Lee V, et al. Conserved Lysine Acetylation within the Microtubule-Binding Domain Regulates MAP2/Tau Family Members. PLoS ONE. 2016;11:e0168913 pubmed publisher
  7. Atasoy İ, Dursun E, Gezen Ak D, Metin Armağan D, Ozturk M, Yilmazer S. Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons. J Chem Neuroanat. 2017;80:19-26 pubmed publisher
  8. López de Maturana R, Lang V, Zubiarrain A, Sousa A, Vázquez N, Gorostidi A, et al. Mutations in LRRK2 impair NF-κB pathway in iPSC-derived neurons. J Neuroinflammation. 2016;13:295 pubmed
  9. Yadav P, Selvaraj B, Bender F, Behringer M, Moradi M, Sivadasan R, et al. Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling. Acta Neuropathol. 2016;132:93-110 pubmed publisher
  10. Collazos Castro J, García Rama C, Alves Sampaio A. Glial progenitor cell migration promotes CNS axon growth on functionalized electroconducting microfibers. Acta Biomater. 2016;35:42-56 pubmed publisher
  11. Peng Y, Kim M, Hullinger R, O Riordan K, Burger C, Pehar M, et al. Improved proteostasis in the secretory pathway rescues Alzheimer's disease in the mouse. Brain. 2016;139:937-52 pubmed publisher
  12. Taniguchi Watanabe S, Arai T, Kametani F, Nonaka T, Masuda Suzukake M, Tarutani A, et al. Biochemical classification of tauopathies by immunoblot, protein sequence and mass spectrometric analyses of sarkosyl-insoluble and trypsin-resistant tau. Acta Neuropathol. 2016;131:267-280 pubmed publisher
  13. Lee I, Jung K, Kim I, Lee H, Kim M, Yun S, et al. Human neural stem cells alleviate Alzheimer-like pathology in a mouse model. Mol Neurodegener. 2015;10:38 pubmed publisher
  14. Corbel C, Zhang B, Le Parc A, Baratte B, Colas P, Couturier C, et al. Tamoxifen inhibits CDK5 kinase activity by interacting with p35/p25 and modulates the pattern of tau phosphorylation. Chem Biol. 2015;22:472-482 pubmed publisher
  15. Takeuchi R, Toyoshima Y, Tada M, Tanaka H, Shimizu H, Shiga A, et al. Globular Glial Mixed Four Repeat Tau and TDP-43 Proteinopathy with Motor Neuron Disease and Frontotemporal Dementia. Brain Pathol. 2016;26:82-94 pubmed publisher
  16. Melis V, Zabke C, Stamer K, Magbagbeolu M, Schwab K, Marschall P, et al. Different pathways of molecular pathophysiology underlie cognitive and motor tauopathy phenotypes in transgenic models for Alzheimer's disease and frontotemporal lobar degeneration. Cell Mol Life Sci. 2015;72:2199-222 pubmed publisher
  17. Denton K, Xu C, Li X. Modeling Axonal Phenotypes with Human Pluripotent Stem Cells. Methods Mol Biol. 2016;1353:309-21 pubmed publisher