This is a Validated Antibody Database (VAD) review about chicken EN1, based on 4 published articles (read how Labome selects the articles), using EN1 antibody in all methods. It is aimed to help Labome visitors find the most suited EN1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
EN1 synonym: En-1; Gg-En-1; en-3; homeobox protein engrailed-1; Engrailed-3; engrailed protein; homeobox protein en-1

Developmental Studies Hybridoma Bank
mouse monoclonal (4G11)
  • immunohistochemistry - frozen section; mouse; 1:50; loading ...; fig s7e
In order to identify a novel mechanism of neuro-vascular communication to control vascular development in the spinal cord, Developmental Studies Hybridoma Bank EN1 antibody (DSHB, 4G11) was used in immunohistochemistry - frozen section on mouse samples at 1:50 (fig s7e). Nat Commun (2017) ncbi
mouse monoclonal (4G11)
  • immunohistochemistry - frozen section; mouse; 1:1000; loading ...; fig s6b
In order to study regulation of Notch1 in ventral neural stem/progenitor cells of the developing spinal cord, Developmental Studies Hybridoma Bank EN1 antibody (DSHB, 4G11-c) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig s6b). Sci Rep (2016) ncbi
mouse monoclonal (4G11)
  • immunohistochemistry; mouse; 1:50
In order to investigate the role of Pax6 in neuronal development, Developmental Studies Hybridoma Bank EN1 antibody (Developmental Studies Hybridoma Bank developed under the auspices, 4G11) was used in immunohistochemistry on mouse samples at 1:50. Dev Biol (2016) ncbi
mouse monoclonal (4G11)
  • immunohistochemistry - frozen section; mouse; 1:25; loading ...; fig 6k
Developmental Studies Hybridoma Bank EN1 antibody (DSHB, 4g11) was used in immunohistochemistry - frozen section on mouse samples at 1:25 (fig 6k). Dev Dyn (2015) ncbi
Articles Reviewed
  1. Himmels P, Paredes I, Adler H, Karakatsani A, Luck R, Marti H, et al. Motor neurons control blood vessel patterning in the developing spinal cord. Nat Commun. 2017;8:14583 pubmed publisher
  2. Li Y, Tzatzalos E, Kwan K, Grumet M, Cai L. Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development. Sci Rep. 2016;6:38665 pubmed publisher
  3. Huettl R, Eckstein S, Stahl T, Petricca S, Ninkovic J, Götz M, et al. Functional dissection of the Pax6 paired domain: Roles in neural tube patterning and peripheral nervous system development. Dev Biol. 2016;413:86-103 pubmed publisher
  4. Zou M, Luo H, Xiang M. Selective neuronal lineages derived from Dll4-expressing progenitors/precursors in the retina and spinal cord. Dev Dyn. 2015;244:86-97 pubmed publisher