This is a Validated Antibody Database (VAD) review about bovine RPS27A, based on 50 published articles (read how Labome selects the articles), using RPS27A antibody in all methods. It is aimed to help Labome visitors find the most suited RPS27A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Invitrogen
domestic rabbit polyclonal |
| Invitrogen RPS27A antibody (Fisher Scientific, PA1-10023) was used in immunohistochemistry - free floating section on mouse samples at 1:2000 (fig s7b). Mol Neurodegener (2022) ncbi |
mouse monoclonal (Ubi-1) |
| Invitrogen RPS27A antibody (Invitrogen, 13-1600) was used in western blot on human samples at 1:2000 (fig 6b). EMBO Mol Med (2019) ncbi |
domestic rabbit polyclonal |
| In order to explore the contribution of STEP61 to the therapeutic effects of D-serine in patients with schizophrenia, Invitrogen RPS27A antibody (Thermo Scientific, PA1-10023) was used in western blot on rat samples at 1:5000 (fig 6b). Mol Neurobiol (2018) ncbi |
mouse monoclonal (Ubi-1) |
| In order to investigate the role of Ser25 phosphorylation in subcellular localization of Annexin A2 and its interaction with mRNP complexes, Invitrogen RPS27A antibody (Invitrogen, 131600) was used in western blot on rat samples (fig 4d). FEBS Open Bio (2017) ncbi |
mouse monoclonal (Ubi-1) | In order to find the E3 ubiquitin ligase Mule is essential for cardiac homeostasis by regulating mitochondrial function via maintenance of Pgc-1alpha and Pink1 expression and persistent negative regulation of c-Myc, Invitrogen RPS27A antibody (Thermo Fisher, 13-1600) was used . Sci Rep (2017) ncbi | |
mouse monoclonal (Ubi-1) |
| In order to describe a function for NQO1 in stabilizing HIF-1alpha, Invitrogen RPS27A antibody (Invitrogen, 131600) was used in western blot on human samples at 1:500 (fig s7a). Nat Commun (2016) ncbi |
mouse monoclonal (Ubi-1) |
| In order to study LRRK2 polymorphisms associated with Parkinson's disease, Invitrogen RPS27A antibody (Invitrogen, 13-1600) was used in western blot on human samples (fig 5). Neurobiol Dis (2017) ncbi |
mouse monoclonal (Ubi-1) |
| In order to propose that neonatal splice variant of CaV1.2 channels contribute to heart disease, Invitrogen RPS27A antibody (Invitrogen, 13-1600) was used in western blot on human samples at 1:1000 (fig 3f). Sci Rep (2016) ncbi |
mouse monoclonal (Ubi-1) |
| In order to utilize a Huntington's disease mouse model to show specific binding profiles and steady-state ubiquitin levels with disease development when comparing mHTT antibodies, Invitrogen RPS27A antibody (Invitrogen, 13.1600) was used in immunohistochemistry - frozen section on mouse samples at 1:1000 (fig 2). PLoS ONE (2016) ncbi |
mouse monoclonal (Ubi-1) |
| In order to analyze regulation of V-ATPase and the lysosomal-autophagic pathway by Drosophila Mitf, Invitrogen RPS27A antibody (ThermoFisher Scientific, 13-1600) was used in immunocytochemistry on fruit fly |
mouse monoclonal (Ubi-1) |
| In order to assess an increase in sensitivity to Salmonella but no affect to proinflammatory NF-kappaB signaling by optineurin deficiency in mice, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunohistochemistry - paraffin section on mouse samples (fig s1). Eur J Immunol (2016) ncbi |
mouse monoclonal (Ubi-1) |
| In order to study the role of 14-3-3sigma in tumor metabolism, Invitrogen RPS27A antibody (Life Technologies, 13-1600) was used in western blot on human samples (fig 5e). Nat Commun (2015) ncbi |
mouse monoclonal (Ubi-1) |
| In order to report that Tat-activating regulatory DNA-binding protein-43 regulates the synthesis of the interleukin-6 and -10 splicing activating compartment, Invitrogen RPS27A antibody (Life Technologies, 13-160) was used in immunohistochemistry - paraffin section on human samples at 1:100 and in immunocytochemistry on human samples . Nat Commun (2015) ncbi |
mouse monoclonal (Ubi-1) |
| In order to identify the mechanistic link between Cullin neddylation and Myc ubiquitination/degradation, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on human samples . Nat Commun (2014) ncbi |
mouse monoclonal (Ubi-1) |
| In order to demonstrate a tumor-suppressive role for SLAP in colorectal cancer, Invitrogen RPS27A antibody (life technologies, 13-1600) was used in western blot on human samples at 1:1000 (fig 5). Nat Commun (2014) ncbi |
mouse monoclonal (Ubi-1) |
| In order to test if targeting the ectodomain of E-cadherin is an effective treatment of HER2-positive breast cancers, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on human samples (fig 5). Clin Cancer Res (2013) ncbi |
mouse monoclonal (Ubi-1) |
| In order to elucidate mechanisms that control stability of p53 mutants, Invitrogen RPS27A antibody (Zymed, monoclonal mix made of P4D1, SCBT and 13-1600) was used in western blot on human samples (fig 1). Cell Cycle (2013) ncbi |
mouse monoclonal (Ubi-1) |
| In order to investigate how p300 affects protein aggregation, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunocytochemistry on African green monkey samples , in western blot on African green monkey samples , in immunocytochemistry on human samples , in immunohistochemistry on human samples and in western blot on human samples . PLoS ONE (2012) ncbi |
mouse monoclonal (Ubi-1) |
| In order to elucidate NEMO binding to linear ubiquitin chains of different lengths, Invitrogen RPS27A antibody (Invitrogen, 13-1600) was used in western blot on human samples (fig 3). J Biol Chem (2012) ncbi |
mouse monoclonal (Ubi-1) |
| In order to show that optineurin colocalizes with ubiquitin in Marinesco bodies, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunohistochemistry on human samples at 1:500 (fig 1). Acta Neuropathol (2012) ncbi |
mouse monoclonal (Ubi-1) |
| In order to evaluate molecular signatures of an individual cell types in patients with of Alzheimer's disease, Invitrogen RPS27A antibody (Invitrogen, 13-1600) was used in immunohistochemistry on human samples (fig 1). Neurobiol Dis (2012) ncbi |
mouse monoclonal (Ubi-1) |
| In order to test if small molecules can restore p27 expression in cancer cells, Invitrogen RPS27A antibody (Zymed Laboratories, 13-1600) was used in western blot on human samples at 1:2000 (fig 5g). BMC Biol (2010) ncbi |
mouse monoclonal (Ubi-1) |
| In order to test if phosphorylation of ATXN1 at Ser776 is important for pathogenesis of SCA1, Invitrogen RPS27A antibody (Invitrogen, 13?C1600) was used in immunohistochemistry on mouse samples at 1:250 (fig 4). Neuron (2010) ncbi |
mouse monoclonal (Ubi-1) |
| In order to identify amyloid precursor-like protein 2 as a partner protein for ataxin-7, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunocytochemistry on human samples at 1:50 (fig 4) and in immunohistochemistry on human samples at 1:50 (fig 4). Neurobiol Dis (2011) ncbi |
mouse monoclonal (Ubi-1) |
| In order to explore the role of autophaghy in a Drosophila model of Dentatorubral-pallidoluysian Atrophy, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunohistochemistry on fruit fly |
mouse monoclonal (Ubi-1) |
| In order to identify proteins that bind phosphorylated and unphosphorylated LRP1, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on rat samples (fig 6). Proteomics (2009) ncbi |
mouse monoclonal (Ubi-1) |
| In order to identify TRP32 as a subunit of the 26 S proteasome, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on mouse samples (fig 6). J Biol Chem (2009) ncbi |
mouse monoclonal (Ubi-1) |
| In order to discuss methods to assess the contribution of cardiomyocyte autophagy to ventricular remodeling and disease pathogenesis, Invitrogen RPS27A antibody (Zymed, 131600) was used in immunohistochemistry on mouse samples at 1:500. Methods Enzymol (2009) ncbi |
mouse monoclonal (Ubi-1) |
| In order to study the relationship between Rad33 and the Rad4-Rad23 during DNA repair, Invitrogen RPS27A antibody (Zymed laboratories, 13-1600) was used in western blot on Saccharomycetales samples . DNA Repair (Amst) (2008) ncbi |
mouse monoclonal (Ubi-1) |
| In order to identify markers for cellular differentiation, Invitrogen RPS27A antibody (Zymed Laboratories, 13-1600) was used in immunocytochemistry on human samples at 4,000 ug/ml. Rapid Commun Mass Spectrom (2008) ncbi |
mouse monoclonal (Ubi-1) |
| In order to identify deubiquitinating enzymes participate in regulating mitochondrial dynamics in mammalian cells, Invitrogen RPS27A antibody (Zymed Laboratories, Ubi-1) was used in western blot on human samples . Mol Biol Cell (2008) ncbi |
mouse monoclonal (Ubi-1) | In order to characterize human glucokinase isoforms, Invitrogen RPS27A antibody (Zymed, 13-1600) was used . J Biol Chem (2007) ncbi | |
mouse monoclonal (Ubi-1) |
| In order to show that PDLIM2 negatively regulates NF-kappaB activity, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on mouse samples (fig 1). Nat Immunol (2007) ncbi |
mouse monoclonal (Ubi-1) |
| In order to assess geldanamycin-induced cell cycle regulation in glioblastoma cells, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on human samples at 1:1000 (fig 7). Biochem Pharmacol (2007) ncbi |
mouse monoclonal (Ubi-1) |
| In order to show that MEKK1 is required for thymus-dependent antigen-induced B cell proliferation and antibody production, Invitrogen RPS27A antibody (Zymed, 13- 1600) was used in western blot on mouse samples (fig 4d). Nat Immunol (2007) ncbi |
mouse monoclonal (Ubi-1) |
| In order to discuss methods to study protein ubiquitylation, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on human samples . Methods Enzymol (2005) ncbi |
mouse monoclonal (Ubi-1) |
| In order to investigate the mechanisms of Chk1 regulation in glioblastoma cells using geldanamycin, Invitrogen RPS27A antibody (ZYMED, 13-1600) was used in western blot on human samples at 1:1000 (fig 5). Biochem Biophys Res Commun (2005) ncbi |
mouse monoclonal (Ubi-1) |
| In order to assess if acquired Fanconi anemia protein dysfunction contributes to cytogenetic instability in secondary acute myelogenous leukemia cells, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunoprecipitation on human samples . Blood (2003) ncbi |
mouse monoclonal (Ubi-1) |
| In order to report that BAG-1 is a substrate of the CHIP ubiquitin ligase in vitro and in vivo, Invitrogen RPS27A antibody (Zymed, 131600) was used in immunoprecipitation on human samples (fig 2). J Biol Chem (2002) ncbi |
mouse monoclonal (Ubi-1) |
| In order to investigate cellular changes when T47D cells are treated with epidermal growth factor, Invitrogen RPS27A antibody (Zymed, 13?C1600) was used in western blot on human samples (fig 8). J Biol Chem (2002) ncbi |
mouse monoclonal (Ubi-1) |
| In order to identify proteins induced by lithium-pilocarpine-mediated status epilepticus, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunohistochemistry on rat samples at 1:200 (tbl 1). Brain Res (2002) ncbi |
mouse monoclonal (Ubi-1) |
| In order to show that IFNgamma treatment results in increased targeting of PPARgamma to the ubiquitin-proteasome system, Invitrogen RPS27A antibody (Zymed Laboratories, 13-1600) was used in western blot on mouse samples (fig 2). J Biol Chem (2002) ncbi |
mouse monoclonal (Ubi-1) |
| In order to report that BRCA1 has ubiquitin protein ligase activity and that cancer-predisposing mutations within the BRCA1 RING domain abolish its ubiquitin ligase activity, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on human samples (fig 2). Proc Natl Acad Sci U S A (2001) ncbi |
mouse monoclonal (Ubi-1) |
| In order to study the relationship between ubiquitinated Cis and the erythropoietin receptor, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in western blot on rhesus macaque samples (fig 5). J Biol Chem (1998) ncbi |
mouse monoclonal (Ubi-1) |
| In order to characterize mouse msos1 and msos2, Invitrogen RPS27A antibody (Zymed, 13-1600) was used in immunoprecipitation on domestic rabbit samples (fig 5d). Mol Cell Biol (1997) ncbi |
Novus Biologicals
mouse monoclonal (Ubi-1) |
| Novus Biologicals RPS27A antibody (Novus, NB300-130) was used in western blot on mouse samples (fig 2d). Cells (2021) ncbi |
mouse monoclonal (Ubi-1) |
| Novus Biologicals RPS27A antibody (Novus, NB300-130) was used in western blot on human samples at 1:1000 (fig 4e). Nature (2019) ncbi |
mouse monoclonal (Ubi-1) |
| Novus Biologicals RPS27A antibody (Novus Biological, NB300-130) was used in western blot on human samples (fig 7c). J Clin Invest (2018) ncbi |
mouse monoclonal (Ubi-1) |
| In order to demonstrate that OTULIN is essential for preventing TNF-associated systemic inflammation in humans and mice, Novus Biologicals RPS27A antibody (novus Biologicals, NB300-130) was used in western blot on human samples (fig 1). Cell (2016) ncbi |
mouse monoclonal (Ubi-1) |
| In order to study the effect of G2019S on neuronal subtypes, Novus Biologicals RPS27A antibody (Novus Biologicals, NB300-130) was used in immunocytochemistry on human samples (fig 3). Stem Cell Reports (2015) ncbi |
Articles Reviewed
- Marvin Guy L, Duncan P, Wagnière S, Antille N, Porta N, Affolter M, et al. Rapid identification of differentiation markers from whole epithelial cells by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry and statistical analysis. Rapid Commun Mass Spectrom. 2008;22:1099-108 pubmed publisher
- Bjørkhaug L, Molnes J, Søvik O, Njølstad P, Flatmark T. Allosteric activation of human glucokinase by free polyubiquitin chains and its ubiquitin-dependent cotranslational proteasomal degradation. J Biol Chem. 2007;282:22757-64 pubmed
- Tanaka T, Grusby M, Kaisho T. PDLIM2-mediated termination of transcription factor NF-kappaB activation by intranuclear sequestration and degradation of the p65 subunit. Nat Immunol. 2007;8:584-91 pubmed
- Nomura N, Nomura M, Newcomb E, Zagzag D. Geldanamycin induces G2 arrest in U87MG glioblastoma cells through downregulation of Cdc2 and cyclin B1. Biochem Pharmacol. 2007;73:1528-36 pubmed
- Gallagher E, Enzler T, Matsuzawa A, Anzelon Mills A, Otero D, Holzer R, et al. Kinase MEKK1 is required for CD40-dependent activation of the kinases Jnk and p38, germinal center formation, B cell proliferation and antibody production. Nat Immunol. 2007;8:57-63 pubmed
- Bloom J, Pagano M. Experimental tests to definitively determine ubiquitylation of a substrate. Methods Enzymol. 2005;399:249-66 pubmed
- Nomura M, Nomura N, Yamashita J. Geldanamycin-induced degradation of Chk1 is mediated by proteasome. Biochem Biophys Res Commun. 2005;335:900-5 pubmed
- Lensch M, Tischkowitz M, Christianson T, Reifsteck C, Speckhart S, Jakobs P, et al. Acquired FANCA dysfunction and cytogenetic instability in adult acute myelogenous leukemia. Blood. 2003;102:7-16 pubmed
- Alberti S, Demand J, Esser C, Emmerich N, Schild H, Hohfeld J. Ubiquitylation of BAG-1 suggests a novel regulatory mechanism during the sorting of chaperone substrates to the proteasome. J Biol Chem. 2002;277:45920-7 pubmed
- Kassenbrock C, Hunter S, Garl P, Johnson G, Anderson S. Inhibition of Src family kinases blocks epidermal growth factor (EGF)-induced activation of Akt, phosphorylation of c-Cbl, and ubiquitination of the EGF receptor. J Biol Chem. 2002;277:24967-75 pubmed
- Tan Z, Sankar R, Tu W, Shin D, Liu H, Wasterlain C, et al. Immunohistochemical study of p53-associated proteins in rat brain following lithium-pilocarpine status epilepticus. Brain Res. 2002;929:129-38 pubmed
- Floyd Z, Stephens J. Interferon-gamma-mediated activation and ubiquitin-proteasome-dependent degradation of PPARgamma in adipocytes. J Biol Chem. 2002;277:4062-8 pubmed
- Ruffner H, Joazeiro C, Hemmati D, Hunter T, Verma I. Cancer-predisposing mutations within the RING domain of BRCA1: loss of ubiquitin protein ligase activity and protection from radiation hypersensitivity. Proc Natl Acad Sci U S A. 2001;98:5134-9 pubmed
- Verdier F, Chretien S, Muller O, Varlet P, Yoshimura A, Gisselbrecht S, et al. Proteasomes regulate erythropoietin receptor and signal transducer and activator of transcription 5 (STAT5) activation. Possible involvement of the ubiquitinated Cis protein. J Biol Chem. 1998;273:28185-90 pubmed
- Nielsen K, Papageorge A, Vass W, Willumsen B, Lowy D. The Ras-specific exchange factors mouse Sos1 (mSos1) and mSos2 are regulated differently: mSos2 contains ubiquitination signals absent in mSos1. Mol Cell Biol. 1997;17:7132-8 pubmed
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