This is a Validated Antibody Database (VAD) review about bovine PXN, based on 25 published articles (read how Labome selects the articles), using PXN antibody in all methods. It is aimed to help Labome visitors find the most suited PXN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Abcam
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; mouse; 1:250; loading ...; fig s2a
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on mouse samples at 1:250 (fig s2a). Nat Commun (2021) ncbi
domestic rabbit monoclonal (Y113)
  • western blot; human; loading ...; fig 2g
Abcam PXN antibody (Abcam, ab32084) was used in western blot on human samples (fig 2g). Mol Ther Nucleic Acids (2021) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; loading ...; fig 2s1b
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples (fig 2s1b). elife (2019) ncbi
domestic rabbit monoclonal (Y113)
  • western blot; human; 1:3000; loading ...; fig 6c
Abcam PXN antibody (Abcam, ab32084) was used in western blot on human samples at 1:3000 (fig 6c). Nat Commun (2019) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:800; loading ...; fig 3a
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:800 (fig 3a). Nat Cell Biol (2019) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; fig 1f
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples (fig 1f). J Exp Med (2019) ncbi
domestic rabbit monoclonal (Y113)
  • immunohistochemistry; mouse; 1:200; loading ...; fig s1a
Abcam PXN antibody (Abcam, Y113) was used in immunohistochemistry on mouse samples at 1:200 (fig s1a). Development (2017) ncbi
domestic rabbit monoclonal (Y113)
  • western blot; human; 1:1000; loading ...; fig 3i, 6c
Abcam PXN antibody (Abcam, ab32084) was used in western blot on human samples at 1:1000 (fig 3i, 6c). Sci Signal (2017) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:200; loading ...; fig 7A
In order to establish that Smurf1 controls Kindlin-2 protein levels in cells and hinders integrin activation, Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:200 (fig 7A). J Cell Biol (2017) ncbi
domestic rabbit monoclonal (Y113)
  • immunohistochemistry; human; loading ...; fig 8d
  • western blot; human; loading ...; fig s8a
In order to study factors that allow tumor cells to metastasize, Abcam PXN antibody (Abcam, 32084) was used in immunohistochemistry on human samples (fig 8d) and in western blot on human samples (fig s8a). Mol Biol Cell (2017) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; mouse; loading ...; fig 3e
  • western blot; mouse; 1:300; loading ...; fig 3a
Abcam PXN antibody (Abcam, Y118) was used in immunocytochemistry on mouse samples (fig 3e) and in western blot on mouse samples at 1:300 (fig 3a). Cell Commun Signal (2017) ncbi
domestic rabbit monoclonal (Y113)
  • western blot; human; loading ...; fig 3c
In order to assess the effects of thrombomodulin on monocyte differentiation, Abcam PXN antibody (Abcam, ab32084) was used in western blot on human samples (fig 3c). Sci Rep (2016) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; loading ...; fig s6b
  • western blot; human; loading ...; fig s7b
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples (fig s6b) and in western blot on human samples (fig s7b). Oncotarget (2016) ncbi
domestic rabbit monoclonal (Y113)
  • western blot; human; 1:200; fig st1
In order to identify and characterize alterations in signal transduction that occur during the development Lapatinib resistance, Abcam PXN antibody (abcam (Epitomics), ab32084 (1500-1)) was used in western blot on human samples at 1:200 (fig st1). Nat Commun (2016) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:100; loading ...; fig 6d
In order to test if prolactin phosphorylates NEK3 to promote migration of breast cancer cells, Abcam PXN antibody (Abcam, 32084) was used in immunocytochemistry on human samples at 1:100 (fig 6d). J Biol Chem (2016) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; fig 3a
In order to propose that ColXVII-actinin4 complexes at the lamella of a moving keratinocyte regulate actin dynamics and regulate the direction of cell movement, Abcam PXN antibody (Abcam, Y113) was used in immunocytochemistry on human samples (fig 3a). FASEB J (2016) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:200; fig 3
In order to study regulation of adhesion and cell migration by recruitment to the leading edge by human phosphatase CDC14A, Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:200 (fig 3). Proc Natl Acad Sci U S A (2016) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; mouse; loading ...; fig 5
  • immunocytochemistry; human; 1:2000; loading ...; fig 1b, 5
  • western blot; human; 1:5000; loading ...; fig 1d
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on mouse samples (fig 5), in immunocytochemistry on human samples at 1:2000 (fig 1b, 5) and in western blot on human samples at 1:5000 (fig 1d). Biophys J (2015) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human; 1:2000
  • immunocytochemistry; mouse; 1:2000
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples at 1:2000 and in immunocytochemistry on mouse samples at 1:2000. PLoS ONE (2014) ncbi
domestic rabbit monoclonal (Y113)
  • immunocytochemistry; human
Abcam PXN antibody (Abcam, ab32084) was used in immunocytochemistry on human samples . Stem Cells (2013) ncbi
Santa Cruz Biotechnology
mouse monoclonal (177)
  • western blot; mouse; 1:200; fig 3
Santa Cruz Biotechnology PXN antibody (santa Cruz, sc-136297) was used in western blot on mouse samples at 1:200 (fig 3). Mol Cancer (2015) ncbi
mouse monoclonal (177)
  • western blot; human; 1:1000; fig 4
In order to report that Koelreuteria formosana ethanolic extract inhibits the invasion and migration of renal cell carcinoma cells, Santa Cruz Biotechnology PXN antibody (Santa Cruz Biotechnology, sc-136297) was used in western blot on human samples at 1:1000 (fig 4). Mol Med Rep (2014) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 4i
Cell Signaling Technology PXN antibody (Cell Signaling Technolog, 2542) was used in western blot on human samples at 1:1000 (fig 4i). Nat Commun (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig s9b
In order to examine whether p140Cap protein levels in ERBB2-amplified breast cancer patients correlate with survival, Cell Signaling Technology PXN antibody (Cell Signaling, 2542) was used in western blot on human samples at 1:1000 (fig s9b). Nat Commun (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; fig 6
In order to study regulation of myofiber stretch and integrin-mediated adhesion due to localized LoxL3-dependent fibronectin oxidation, Cell Signaling Technology PXN antibody (Cell Signaling, 2542) was used in western blot on human samples at 1:1000 (fig 6). Dev Cell (2016) ncbi
Articles Reviewed
  1. Kim J, Hwang K, Dang B, Eom M, Kong I, Gwack Y, et al. Insulin-activated store-operated Ca2+ entry via Orai1 induces podocyte actin remodeling and causes proteinuria. Nat Commun. 2021;12:6537 pubmed publisher
  2. Zhang L, Yao L, Zhou W, Tian J, Ruan B, Lu Z, et al. miR-497 defect contributes to gastric cancer tumorigenesis and progression via regulating CDC42/ITGB1/FAK/PXN/AKT signaling. Mol Ther Nucleic Acids. 2021;25:567-577 pubmed publisher
  3. Yin H, Wang J, Li H, Yu Y, Wang X, Lu L, et al. Extracellular matrix protein-1 secretory isoform promotes ovarian cancer through increasing alternative mRNA splicing and stemness. Nat Commun. 2021;12:4230 pubmed publisher
  4. Shi K, Yin X, Cai M, Yan Y, Jia C, Ma P, et al. PAX8 regulon in human ovarian cancer links lineage dependency with epigenetic vulnerability to HDAC inhibitors. elife. 2019;8: pubmed publisher
  5. Frank A, Ebersberger S, Fink A, Lampe S, Weigert A, Schmid T, et al. Apoptotic tumor cell-derived microRNA-375 uses CD36 to alter the tumor-associated macrophage phenotype. Nat Commun. 2019;10:1135 pubmed publisher
  6. Moro A, Driscoll T, Boraas L, Armero W, Kasper D, Baeyens N, et al. MicroRNA-dependent regulation of biomechanical genes establishes tissue stiffness homeostasis. Nat Cell Biol. 2019;21:348-358 pubmed publisher
  7. Gao Q, Yang Z, Xu S, Li X, Yang X, Jin P, et al. Heterotypic CAF-tumor spheroids promote early peritoneal metastatis of ovarian cancer. J Exp Med. 2019;216:688-703 pubmed publisher
  8. Rashid M, Belmont J, Carpenter D, Turner C, Olson E. Neural-specific deletion of the focal adhesion adaptor protein paxillin slows migration speed and delays cortical layer formation. Development. 2017;144:4002-4014 pubmed publisher
  9. Amin E, Liu Y, Deng S, Tan K, Chudgar N, Mayo M, et al. The RNA-editing enzyme ADAR promotes lung adenocarcinoma migration and invasion by stabilizing FAK. Sci Signal. 2017;10: pubmed publisher
  10. Wei X, Wang X, Zhan J, Chen Y, Fang W, Zhang L, et al. Smurf1 inhibits integrin activation by controlling Kindlin-2 ubiquitination and degradation. J Cell Biol. 2017;216:1455-1471 pubmed publisher
  11. Mekhdjian A, Kai F, Rubashkin M, Prahl L, Przybyla L, McGregor A, et al. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. Mol Biol Cell. 2017;28:1467-1488 pubmed publisher
  12. Grasso S, Chapelle J, Salemme V, Aramu S, Russo I, Vitale N, et al. The scaffold protein p140Cap limits ERBB2-mediated breast cancer progression interfering with Rac GTPase-controlled circuitries. Nat Commun. 2017;8:14797 pubmed publisher
  13. Shen X, Jia Z, D Alonzo D, Wang X, Bruder E, Emch F, et al. HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures. Cell Commun Signal. 2017;15:2 pubmed publisher
  14. Tsai C, Lin Y, Huang C, Shih C, Tsai Y, Tsao N, et al. Thrombomodulin regulates monocye differentiation via PKC? and ERK1/2 pathway in vitro and in atherosclerotic artery. Sci Rep. 2016;6:38421 pubmed publisher
  15. Ross Adams H, Ball S, Lawrenson K, Halim S, Russell R, Wells C, et al. HNF1B variants associate with promoter methylation and regulate gene networks activated in prostate and ovarian cancer. Oncotarget. 2016;7:74734-74746 pubmed publisher
  16. Treindl F, Ruprecht B, Beiter Y, Schultz S, Döttinger A, Staebler A, et al. A bead-based western for high-throughput cellular signal transduction analyses. Nat Commun. 2016;7:12852 pubmed publisher
  17. Harrington K, Clevenger C. Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration. J Biol Chem. 2016;291:21388-21406 pubmed
  18. Kraft Sheleg O, Zaffryar Eilot S, Genin O, Yaseen W, Soueid Baumgarten S, Kessler O, et al. Localized LoxL3-Dependent Fibronectin Oxidation Regulates Myofiber Stretch and Integrin-Mediated Adhesion. Dev Cell. 2016;36:550-61 pubmed publisher
  19. Hiroyasu S, Colburn Z, Jones J. A hemidesmosomal protein regulates actin dynamics and traction forces in motile keratinocytes. FASEB J. 2016;30:2298-310 pubmed publisher
  20. Chen N, Uddin B, Voit R, Schiebel E. Human phosphatase CDC14A is recruited to the cell leading edge to regulate cell migration and adhesion. Proc Natl Acad Sci U S A. 2016;113:990-5 pubmed publisher
  21. Darr J, Klochendler A, Isaac S, Geiger T, Geiger T, Eden A. Phosphoproteomic analysis reveals Smarcb1 dependent EGFR signaling in Malignant Rhabdoid tumor cells. Mol Cancer. 2015;14:167 pubmed publisher
  22. Fuhrmann A, Engler A. The cytoskeleton regulates cell attachment strength. Biophys J. 2015;109:57-65 pubmed publisher
  23. Lin C, Chen P, Hsu L, Kuo D, Chu S, Hsieh Y. Inhibition of the invasion and migration of renal carcinoma 786‑o‑si3 cells in vitro and in vivo by Koelreuteria formosana extract. Mol Med Rep. 2014;10:3334-42 pubmed publisher
  24. Fuhrmann A, Li J, Chien S, Engler A. Cation type specific cell remodeling regulates attachment strength. PLoS ONE. 2014;9:e102424 pubmed publisher
  25. Holle A, Tang X, Vijayraghavan D, Vincent L, Fuhrmann A, Choi Y, et al. In situ mechanotransduction via vinculin regulates stem cell differentiation. Stem Cells. 2013;31:2467-77 pubmed publisher