This is a Validated Antibody Database (VAD) review about bovine PDHA1, based on 21 published articles (read how Labome selects the articles), using PDHA1 antibody in all methods. It is aimed to help Labome visitors find the most suited PDHA1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PDHA1 synonym: PDHA

Knockout validation
Abcam
mouse monoclonal (8D10E6)
  • western blot knockout validation; human; loading ...; fig 3a
Abcam PDHA1 antibody (Abcam, ab110334) was used in western blot knockout validation on human samples (fig 3a). Oncotarget (2016) ncbi
Abcam
mouse monoclonal (8D10E6)
  • immunohistochemistry; Drosophila melanogaster; fig 4f
Abcam PDHA1 antibody (Abcam, ab110334) was used in immunohistochemistry on Drosophila melanogaster samples (fig 4f). Nature (2019) ncbi
mouse monoclonal (9H9AF5)
  • western blot; mouse; 1:1000; loading ...; fig s2n
Abcam PDHA1 antibody (Abcam, ab110330) was used in western blot on mouse samples at 1:1000 (fig s2n). Nature (2017) ncbi
mouse monoclonal (8D10E6)
  • other; Drosophila melanogaster; 1:500; loading ...; fig 4c
  • western blot; Drosophila melanogaster; 1:1000; fig s2
Abcam PDHA1 antibody (Abcam, ab110334) was used in other on Drosophila melanogaster samples at 1:500 (fig 4c) and in western blot on Drosophila melanogaster samples at 1:1000 (fig s2). Sci Rep (2017) ncbi
mouse monoclonal (9H9AF5)
  • western blot; mouse; loading ...; fig 7f
In order to identify posttranscriptional mechanisms that regulate mitochondrial protein expression, Abcam PDHA1 antibody (Abcam, ab110330) was used in western blot on mouse samples (fig 7f). J Cell Biol (2017) ncbi
mouse monoclonal (8D10E6)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 3k
In order to demonstrate that some mitochondrial enzymes associated with the tricarboxylic acid cycle are essential for epigenetic remodeling and transiently localize to the nucleus, Abcam PDHA1 antibody (Abcam, ab110334) was used in immunohistochemistry on mouse samples at 1:100 (fig 3k). Cell (2017) ncbi
mouse monoclonal (8D10E6)
  • western blot knockout validation; human; loading ...; fig 3a
Abcam PDHA1 antibody (Abcam, ab110334) was used in western blot knockout validation on human samples (fig 3a). Oncotarget (2016) ncbi
mouse monoclonal (8D10E6)
  • western blot; human; loading ...; fig 3k
Abcam PDHA1 antibody (Abcam, Ab110334) was used in western blot on human samples (fig 3k). Mol Metab (2016) ncbi
mouse monoclonal (9H9AF5)
  • western blot; mouse; 1:1000; loading ...; fig 1b
Abcam PDHA1 antibody (Abcam, ab110330) was used in western blot on mouse samples at 1:1000 (fig 1b). PLoS ONE (2016) ncbi
mouse monoclonal (8D10E6)
  • immunohistochemistry; Drosophila melanogaster
Abcam PDHA1 antibody (Abcam, ab110334) was used in immunohistochemistry on Drosophila melanogaster samples . Nat Cell Biol (2015) ncbi
mouse monoclonal (8D10E6)
  • western blot; mouse
Abcam PDHA1 antibody (Abcam, ab110334) was used in western blot on mouse samples . J Immunol (2014) ncbi
Invitrogen
mouse monoclonal (9H9AF5)
  • western blot; human; loading ...; fig 4b
Invitrogen PDHA1 antibody (Thermo Fisher Scientific, 459400) was used in western blot on human samples (fig 4b). Cell Rep (2019) ncbi
mouse monoclonal (9H9AF5)
  • western blot; human; 1:500; loading ...; fig 6c
In order to examine whether proteins of energy metabolism can be used as biomarkers of inflammatory myopathies, Invitrogen PDHA1 antibody (Invitrogen, 459400) was used in western blot on human samples at 1:500 (fig 6c). J Transl Med (2017) ncbi
mouse monoclonal (9H9AF5)
  • western blot; mouse; loading ...; fig 4a
In order to report the effects of taurine administration on glycogen repletion and carbohydrate metabolism in the tibialis anterior muscle after endurance exercise, Invitrogen PDHA1 antibody (Invitrogen, 459400) was used in western blot on mouse samples (fig 4a). J Nutr Sci Vitaminol (Tokyo) (2016) ncbi
mouse monoclonal (9H9AF5)
  • western blot; mouse; loading ...
In order to show that E4F1 regulates pyruvate metabolism to maintain skin homeostasis, Invitrogen PDHA1 antibody (Life technologies, 459400) was used in western blot on mouse samples . Proc Natl Acad Sci U S A (2016) ncbi
mouse monoclonal (9H9AF5)
  • western blot; human; loading ...; fig 3e
In order to determine the role of reductive stress in mitochondrial pathogenesis, Invitrogen PDHA1 antibody (Invitrogen, 459400) was used in western blot on human samples (fig 3e). Science (2016) ncbi
mouse monoclonal (9H9AF5)
  • western blot; human; fig s2
In order to study the support of redox homeostasis during anchorage-independent growth by reductive carboxylation, Invitrogen PDHA1 antibody (Thermo Scientific, 459400) was used in western blot on human samples (fig s2). Nature (2016) ncbi
mouse monoclonal (9H9AF5)
  • western blot; mouse
In order to elucidate the mechanisms by which KRAS mutations contribute to lung cancer, Invitrogen PDHA1 antibody (Life Technologies, 459400) was used in western blot on mouse samples . Nature (2016) ncbi
mouse monoclonal (9H9AF5)
  • western blot; human
In order to discuss the metabolic programs that drive tumorigenesis, Invitrogen PDHA1 antibody (Invitrogen, 459400) was used in western blot on human samples . Neuro Oncol (2015) ncbi
mouse monoclonal (8D10E6)
  • western blot; mouse; 1:750; fig 2
In order to test if drugs that alter mitochondrial metabolism also modulate amyotrophic lateral sclerosis progression, Invitrogen PDHA1 antibody (Invitrogen, 456600) was used in western blot on mouse samples at 1:750 (fig 2). PLoS ONE (2012) ncbi
mouse monoclonal (9H9AF5)
  • western blot; human; 1:500; fig 6
In order to test if switching the metabolism from glycolysis towards mitochondrial respiration reduces the growth of colorectal cancer cells and explore the mechanism, Invitrogen PDHA1 antibody (Invitrogen, 459400) was used in western blot on human samples at 1:500 (fig 6). Br J Cancer (2010) ncbi
EMD Millipore
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig 2a
In order to report that E4F1 is a master regulator of the pyruvate dehydrogenase complex, EMD Millipore PDHA1 antibody (Millipore, ABS 204) was used in western blot on mouse samples (fig 2a). Proc Natl Acad Sci U S A (2016) ncbi
Articles Reviewed
  1. Park S, Safi R, Liu X, Baldi R, Liu W, Liu J, et al. Inhibition of ERRα Prevents Mitochondrial Pyruvate Uptake Exposing NADPH-Generating Pathways as Targetable Vulnerabilities in Breast Cancer. Cell Rep. 2019;27:3587-3601.e4 pubmed publisher
  2. Lieber T, Jeedigunta S, Palozzi J, Lehmann R, Hurd T. Mitochondrial fragmentation drives selective removal of deleterious mtDNA in the germline. Nature. 2019;: pubmed publisher
  3. Luongo T, Lambert J, Gross P, Nwokedi M, Lombardi A, Shanmughapriya S, et al. The mitochondrial Na+/Ca2+ exchanger is essential for Ca2+ homeostasis and viability. Nature. 2017;545:93-97 pubmed publisher
  4. Jiang Y, Lin S, Chen J, Tsai H, Hsieh T, Fu C. Electron tomographic analysis reveals ultrastructural features of mitochondrial cristae architecture which reflect energetic state and aging. Sci Rep. 2017;7:45474 pubmed publisher
  5. Schatton D, Pla Martín D, Marx M, Hansen H, Mourier A, Nemazanyy I, et al. CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol. 2017;216:675-693 pubmed publisher
  6. Santacatterina F, Sánchez Aragó M, Catalán García M, Garrabou G, de Arenas C, Grau J, et al. Pyruvate kinase M2 and the mitochondrial ATPase Inhibitory Factor 1 provide novel biomarkers of dermatomyositis: a metabolic link to oncogenesis. J Transl Med. 2017;15:29 pubmed publisher
  7. Nagaraj R, Sharpley M, Chi F, Braas D, Zhou Y, Kim R, et al. Nuclear Localization of Mitochondrial TCA Cycle Enzymes as a Critical Step in Mammalian Zygotic Genome Activation. Cell. 2017;168:210-223.e11 pubmed publisher
  8. Takahashi Y, Tamura Y, Matsunaga Y, Kitaoka Y, Terada S, Hatta H. Effects of Taurine Administration on Carbohydrate Metabolism in Skeletal Muscle during the Post-Exercise Phase. J Nutr Sci Vitaminol (Tokyo). 2016;62:257-264 pubmed
  9. Lacroix M, Rodier G, Kirsh O, Houles T, Delpech H, Seyran B, et al. E4F1 controls a transcriptional program essential for pyruvate dehydrogenase activity. Proc Natl Acad Sci U S A. 2016;113:10998-1003 pubmed publisher
  10. Goguet Rubio P, Seyran B, Gayte L, Bernex F, Sutter A, Delpech H, et al. E4F1-mediated control of pyruvate dehydrogenase activity is essential for skin homeostasis. Proc Natl Acad Sci U S A. 2016;113:11004-9 pubmed publisher
  11. Li Y, Li X, Li X, Zhong Y, Ji Y, Yu D, et al. PDHA1 gene knockout in prostate cancer cells results in metabolic reprogramming towards greater glutamine dependence. Oncotarget. 2016;7:53837-53852 pubmed publisher
  12. Titov D, Cracan V, Goodman R, Peng J, Grabarek Z, Mootha V. Complementation of mitochondrial electron transport chain by manipulation of the NAD+/NADH ratio. Science. 2016;352:231-5 pubmed publisher
  13. Barquissau V, Beuzelin D, Pisani D, Beranger G, Mairal A, Montagner A, et al. White-to-brite conversion in human adipocytes promotes metabolic reprogramming towards fatty acid anabolic and catabolic pathways. Mol Metab. 2016;5:352-365 pubmed publisher
  14. Jiang L, Shestov A, Swain P, Yang C, Parker S, Wang Q, et al. Reductive carboxylation supports redox homeostasis during anchorage-independent growth. Nature. 2016;532:255-8 pubmed publisher
  15. Zhang Y, Zhao Z, Ke B, Wan L, Wang H, Ye J. Induction of Posttranslational Modifications of Mitochondrial Proteins by ATP Contributes to Negative Regulation of Mitochondrial Function. PLoS ONE. 2016;11:e0150454 pubmed publisher
  16. Kerr E, Gaude E, Turrell F, Frezza C, Martins C. Mutant Kras copy number defines metabolic reprogramming and therapeutic susceptibilities. Nature. 2016;531:110-3 pubmed publisher
  17. Teixeira F, Sanchez C, Hurd T, Seifert J, Czech B, Preall J, et al. ATP synthase promotes germ cell differentiation independent of oxidative phosphorylation. Nat Cell Biol. 2015;17:689-96 pubmed publisher
  18. Prabhu A, Sarcar B, Miller C, Kim S, Nakano I, Forsyth P, et al. Ras-mediated modulation of pyruvate dehydrogenase activity regulates mitochondrial reserve capacity and contributes to glioblastoma tumorigenesis. Neuro Oncol. 2015;17:1220-30 pubmed publisher
  19. Caro Maldonado A, Wang R, Nichols A, Kuraoka M, Milasta S, Sun L, et al. Metabolic reprogramming is required for antibody production that is suppressed in anergic but exaggerated in chronically BAFF-exposed B cells. J Immunol. 2014;192:3626-36 pubmed publisher
  20. Miquel E, Cassina A, Martinez Palma L, Bolatto C, Trias E, Gandelman M, et al. Modulation of astrocytic mitochondrial function by dichloroacetate improves survival and motor performance in inherited amyotrophic lateral sclerosis. PLoS ONE. 2012;7:e34776 pubmed publisher
  21. Madhok B, Yeluri S, Perry S, Hughes T, Jayne D. Dichloroacetate induces apoptosis and cell-cycle arrest in colorectal cancer cells. Br J Cancer. 2010;102:1746-52 pubmed publisher