This is a Validated Antibody Database (VAD) review about bovine NPM1, based on 37 published articles (read how Labome selects the articles), using NPM1 antibody in all methods. It is aimed to help Labome visitors find the most suited NPM1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Abcam
mouse monoclonal (FC82291)
  • western blot knockout validation; human; 1:1000; loading ...; fig 4j
Abcam NPM1 antibody (Abcam, Ab10530) was used in western blot knockout validation on human samples at 1:1000 (fig 4j). Nat Commun (2021) ncbi
Abcam
mouse monoclonal (FC82291)
  • immunocytochemistry; human; loading ...; fig 2d
Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on human samples (fig 2d). Sci Adv (2021) ncbi
mouse monoclonal (FC82291)
  • western blot; human; 1:1000; loading ...; fig 2d
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on human samples at 1:1000 (fig 2d). Nat Commun (2021) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; loading ...; fig 3a
  • western blot; human; loading ...; fig 3d
Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on human samples (fig 3a) and in western blot on human samples (fig 3d). EMBO J (2021) ncbi
mouse monoclonal (FC82291)
  • western blot knockout validation; human; 1:1000; loading ...; fig 4j
Abcam NPM1 antibody (Abcam, Ab10530) was used in western blot knockout validation on human samples at 1:1000 (fig 4j). Nat Commun (2021) ncbi
mouse monoclonal (FC82291)
  • immunohistochemistry - frozen section; human; loading ...; fig 1b, 1c
Abcam NPM1 antibody (Abcam, ab10530) was used in immunohistochemistry - frozen section on human samples (fig 1b, 1c). Science (2020) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:50; loading ...; fig 6a
Abcam NPM1 antibody (abcam, ab10530) was used in immunocytochemistry on human samples at 1:50 (fig 6a). Int J Mol Sci (2020) ncbi
mouse monoclonal (FC82291)
  • western blot; human; loading ...; fig 3e
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on human samples (fig 3e). Cell Death Discov (2020) ncbi
mouse monoclonal (FC82291)
  • western blot; mouse; 1:500; loading ...; fig 7d
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on mouse samples at 1:500 (fig 7d). J Biol Chem (2019) ncbi
mouse monoclonal (FC82291)
Abcam NPM1 antibody (abcam, ab10530) was used . Cell Death Differ (2019) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:100; loading ...; fig 1f
In order to show that macroH2A variants aid in maintaining nuclear organization and heterochromatin architecture, Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on human samples at 1:100 (fig 1f). J Cell Sci (2017) ncbi
mouse monoclonal (FC82291)
  • western blot; human; loading ...; fig 1a
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on human samples (fig 1a). Oncotarget (2016) ncbi
mouse monoclonal (FC82291)
  • western blot; human; fig s1
Abcam NPM1 antibody (Abcam, Ab10530) was used in western blot on human samples (fig s1). PLoS ONE (2016) ncbi
mouse monoclonal (FC82291)
  • immunohistochemistry - paraffin section; human; 1:5000; loading ...; fig 6a
  • immunocytochemistry; human; 1:500; loading ...; fig 1a
  • western blot; human; loading ...; fig 1c
Abcam NPM1 antibody (Abcam, ab10530) was used in immunohistochemistry - paraffin section on human samples at 1:5000 (fig 6a), in immunocytochemistry on human samples at 1:500 (fig 1a) and in western blot on human samples (fig 1c). Oncotarget (2016) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:200; fig s2
  • western blot; human; fig 5
  • western blot; mouse; fig 4
Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on human samples at 1:200 (fig s2), in western blot on human samples (fig 5) and in western blot on mouse samples (fig 4). BMC Cell Biol (2015) ncbi
mouse monoclonal (FC82291)
  • western blot; mouse
Abcam NPM1 antibody (Abcam, FC82291) was used in western blot on mouse samples . Breast Cancer Res (2015) ncbi
mouse monoclonal (FC82291)
  • other; human; fig 2
In order to characterize capabilities and potentials of extracellular vesicle (EV) array, Abcam NPM1 antibody (Abcam, ab10530) was used in other on human samples (fig 2). J Extracell Vesicles (2015) ncbi
mouse monoclonal (FC82291)
  • immunoprecipitation; human; loading ...; fig 1a
  • immunocytochemistry; human; loading ...; fig s1
  • western blot; human; loading ...; fig 6a
Abcam NPM1 antibody (Abcam, ab10530) was used in immunoprecipitation on human samples (fig 1a), in immunocytochemistry on human samples (fig s1) and in western blot on human samples (fig 6a). Sci Rep (2015) ncbi
mouse monoclonal (FC82291)
  • immunoprecipitation; human; fig 6c
  • western blot; human; 1:3000; fig 7g
Abcam NPM1 antibody (Abcam, ab10530) was used in immunoprecipitation on human samples (fig 6c) and in western blot on human samples at 1:3000 (fig 7g). Nat Commun (2015) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human
Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on human samples . J Neurochem (2014) ncbi
mouse monoclonal (FC82291)
  • western blot; human; fig 2
Abcam NPM1 antibody (Abcam, ab10530) was used in western blot on human samples (fig 2). Oncotarget (2014) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; mouse
Abcam NPM1 antibody (Abcam, ab10530) was used in immunocytochemistry on mouse samples . Stem Cells Dev (2014) ncbi
Santa Cruz Biotechnology
mouse monoclonal (FC82291)
  • immunocytochemistry; mouse; 1:200; fig s1
Santa Cruz Biotechnology NPM1 antibody (Santa Cruz, SC56622) was used in immunocytochemistry on mouse samples at 1:200 (fig s1). EMBO J (2016) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (D19C1)
  • western blot; human; 1:1000; loading ...; fig 4d
Cell Signaling Technology NPM1 antibody (Cell Signalling Technology, 3520) was used in western blot on human samples at 1:1000 (fig 4d). EMBO Mol Med (2019) ncbi
domestic rabbit monoclonal (D19C1)
  • western blot; human; loading ...; fig 3
In order to report that PPM1D modulates nucleolar formation by regulating NPM phosphorylation, Cell Signaling Technology NPM1 antibody (Cell Signaling, D19C1) was used in western blot on human samples (fig 3). Sci Rep (2016) ncbi
MilliporeSigma
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:1000; loading ...; fig s3c
MilliporeSigma NPM1 antibody (Sigma, B0556) was used in immunocytochemistry on human samples at 1:1000 (fig s3c). Sci Adv (2020) ncbi
mouse monoclonal (FC82291)
  • western blot; mouse; 1:500; loading ...; fig 4c
MilliporeSigma NPM1 antibody (Sigma-Aldrich, B0556) was used in western blot on mouse samples at 1:500 (fig 4c). PLoS Biol (2019) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; mouse; loading ...; fig 2s1b
MilliporeSigma NPM1 antibody (Sigma, B0556) was used in immunocytochemistry on mouse samples (fig 2s1b). elife (2019) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:600; loading ...; fig s7m
MilliporeSigma NPM1 antibody (Sigma-Aldrich, B0556) was used in immunocytochemistry on human samples at 1:600 (fig s7m). Nature (2018) ncbi
mouse monoclonal (FC82291)
  • western blot; mouse; 1:1000; loading ...; fig st3
In order to find the E3 ubiquitin ligase Mule is essential for cardiac homeostasis by regulating mitochondrial function via maintenance of Pgc-1alpha and Pink1 expression and persistent negative regulation of c-Myc, MilliporeSigma NPM1 antibody (Sigma, B0556) was used in western blot on mouse samples at 1:1000 (fig st3). Sci Rep (2017) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:1000; loading ...; fig 2h
  • western blot; human; 1:500; loading ...; fig 2g
MilliporeSigma NPM1 antibody (Sigma-Aldrich, B0556) was used in immunocytochemistry on human samples at 1:1000 (fig 2h) and in western blot on human samples at 1:500 (fig 2g). Proc Natl Acad Sci U S A (2017) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:200; fig 4
In order to investigate the connection between calorie restriction and magnesium, MilliporeSigma NPM1 antibody (Sigma, B0556) was used in immunocytochemistry on human samples at 1:200 (fig 4). Nucleic Acids Res (2016) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; mouse; loading ...; fig 2e
  • western blot; mouse; loading ...; fig 7a
In order to elucidate the function of nucleophosmin 1 in the brain, MilliporeSigma NPM1 antibody (Sigma, B0556) was used in immunocytochemistry on mouse samples (fig 2e) and in western blot on mouse samples (fig 7a). J Biol Chem (2016) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; 1:100; fig 2
MilliporeSigma NPM1 antibody (Sigma-Aldrich, B0556) was used in immunocytochemistry on human samples at 1:100 (fig 2). Oncol Lett (2016) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; mouse; 1:1000; fig 6
MilliporeSigma NPM1 antibody (Sigma, B0556) was used in immunocytochemistry on mouse samples at 1:1000 (fig 6). BMC Dev Biol (2015) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; loading ...; fig 3a
  • immunohistochemistry; human; fig 1a
  • western blot; human; loading ...; fig 6c
  • immunocytochemistry; mouse; loading ...; fig 2e
  • western blot; mouse; loading ...; fig 2d
MilliporeSigma NPM1 antibody (Sigma, FC82291) was used in immunocytochemistry on human samples (fig 3a), in immunohistochemistry on human samples (fig 1a), in western blot on human samples (fig 6c), in immunocytochemistry on mouse samples (fig 2e) and in western blot on mouse samples (fig 2d). Sci Rep (2015) ncbi
mouse monoclonal (FC82291)
  • immunocytochemistry; human; fig 7a
MilliporeSigma NPM1 antibody (Sigma, B0556) was used in immunocytochemistry on human samples (fig 7a). Mol Cell Biol (2015) ncbi
mouse monoclonal (FC82291)
  • western blot; mouse; fig s2c
MilliporeSigma NPM1 antibody (Sigma, FC82291) was used in western blot on mouse samples (fig s2c). Mol Cell Proteomics (2012) ncbi
Articles Reviewed
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  3. Repenning A, Happel D, Bouchard C, Meixner M, Verel Yilmaz Y, Raifer H, et al. PRMT1 promotes the tumor suppressor function of p14ARF and is indicative for pancreatic cancer prognosis. EMBO J. 2021;40:e106777 pubmed publisher
  4. Shi L, Magee P, Fassan M, Sahoo S, Leong H, LEE D, et al. A KRAS-responsive long non-coding RNA controls microRNA processing. Nat Commun. 2021;12:2038 pubmed publisher
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  6. Klein I, Boija A, Afeyan L, Hawken S, Fan M, Dall Agnese A, et al. Partitioning of cancer therapeutics in nuclear condensates. Science. 2020;368:1386-1392 pubmed publisher
  7. Gil L, Niño S, Chi Ahumada E, Rodríguez Leyva I, Guerrero C, Rebolledo A, et al. Perinuclear Lamin A and Nucleoplasmic Lamin B2 Characterize Two Types of Hippocampal Neurons through Alzheimer's Disease Progression. Int J Mol Sci. 2020;21: pubmed publisher
  8. Saini N, Sarin A. Nucleolar localization of the Notch4 intracellular domain underpins its regulation of the cellular response to genotoxic stressors. Cell Death Discov. 2020;6:7 pubmed publisher
  9. Gkatza N, Castro C, Harvey R, Heiß M, Popis M, Blanco S, et al. Cytosine-5 RNA methylation links protein synthesis to cell metabolism. PLoS Biol. 2019;17:e3000297 pubmed publisher
  10. Singh R, Peng S, Viswanath P, Sambandam V, Shen L, Rao X, et al. Non-canonical cMet regulation by vimentin mediates Plk1 inhibitor-induced apoptosis. EMBO Mol Med. 2019;: pubmed publisher
  11. Brody M, Vanhoutte D, Bakshi C, Liu R, Correll R, Sargent M, et al. Disruption of valosin-containing protein activity causes cardiomyopathy and reveals pleiotropic functions in cardiac homeostasis. J Biol Chem. 2019;294:8918-8929 pubmed publisher
  12. Del Rosario B, Kriz A, Del Rosario A, Anselmo A, Fry C, White F, et al. Exploration of CTCF post-translation modifications uncovers Serine-224 phosphorylation by PLK1 at pericentric regions during the G2/M transition. elife. 2019;8: pubmed publisher
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  14. Rai A, Chen J, Selbach M, Pelkmans L. Kinase-controlled phase transition of membraneless organelles in mitosis. Nature. 2018;559:211-216 pubmed publisher
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  16. Dadson K, Hauck L, Hao Z, Grothe D, Rao V, Mak T, et al. The E3 ligase Mule protects the heart against oxidative stress and mitochondrial dysfunction through Myc-dependent inactivation of Pgc-1α and Pink1. Sci Rep. 2017;7:41490 pubmed publisher
  17. Coccia M, Rossi A, Riccio A, Trotta E, Santoro M. Human NF-κB repressing factor acts as a stress-regulated switch for ribosomal RNA processing and nucleolar homeostasis surveillance. Proc Natl Acad Sci U S A. 2017;114:1045-1050 pubmed publisher
  18. Huang M, Garcia J, Thomas D, Zhu L, Nguyen L, Chan S, et al. Autophagy mediates proteolysis of NPM1 and HEXIM1 and sensitivity to BET inhibition in AML cells. Oncotarget. 2016;7:74917-74930 pubmed publisher
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  20. Abraham K, Chan J, Salvi J, Ho B, Hall A, Vidya E, et al. Intersection of calorie restriction and magnesium in the suppression of genome-destabilizing RNA-DNA hybrids. Nucleic Acids Res. 2016;44:8870-8884 pubmed
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  29. Wandrey F, Montellese C, Koos K, Badertscher L, Bammert L, Cook A, et al. The NF45/NF90 Heterodimer Contributes to the Biogenesis of 60S Ribosomal Subunits and Influences Nucleolar Morphology. Mol Cell Biol. 2015;35:3491-503 pubmed publisher
  30. Tancioni I, Miller N, Uryu S, Lawson C, Jean C, Chen X, et al. FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth. Breast Cancer Res. 2015;17:47 pubmed publisher
  31. Jørgensen M, Bæk R, Varming K. Potentials and capabilities of the Extracellular Vesicle (EV) Array. J Extracell Vesicles. 2015;4:26048 pubmed publisher
  32. Arnoldo L, Sgarra R, Chiefari E, Iiritano S, Arcidiacono B, Pegoraro S, et al. A novel mechanism of post-translational modulation of HMGA functions by the histone chaperone nucleophosmin. Sci Rep. 2015;5:8552 pubmed publisher
  33. Wang L, Liu R, Ye P, Wong C, Chen G, Zhou P, et al. Intracellular CD24 disrupts the ARF-NPM interaction and enables mutational and viral oncogene-mediated p53 inactivation. Nat Commun. 2015;6:5909 pubmed publisher
  34. Khalouei S, Chow A, Brown I. Localization of heat shock protein HSPA6 (HSP70B') to sites of transcription in cultured differentiated human neuronal cells following thermal stress. J Neurochem. 2014;131:743-54 pubmed publisher
  35. Hamilton G, Abraham A, Morton J, Sampson O, Pefani D, Khoronenkova S, et al. AKT regulates NPM dependent ARF localization and p53mut stability in tumors. Oncotarget. 2014;5:6142-67 pubmed
  36. Lührig S, Siamishi I, Tesmer Wolf M, Zechner U, Engel W, Nolte J. Lrrc34, a novel nucleolar protein, interacts with npm1 and ncl and has an impact on pluripotent stem cells. Stem Cells Dev. 2014;23:2862-74 pubmed publisher
  37. Tondeleir D, Lambrechts A, Muller M, Jonckheere V, Doll T, Vandamme D, et al. Cells lacking ?-actin are genetically reprogrammed and maintain conditional migratory capacity. Mol Cell Proteomics. 2012;11:255-71 pubmed publisher