This is a Validated Antibody Database (VAD) review about bovine NKX6-1, based on 13 published articles (read how Labome selects the articles), using NKX6-1 antibody in all methods. It is aimed to help Labome visitors find the most suited NKX6-1 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Developmental Studies Hybridoma Bank
mouse monoclonal (F55A12)
  • immunohistochemistry - frozen section; human; 1:50; loading ...; fig e1a
  • flow cytometry; human; 1:50; loading ...; fig 1a, s3c
Developmental Studies Hybridoma Bank NKX6-1 antibody (DHSB, F55A12) was used in immunohistochemistry - frozen section on human samples at 1:50 (fig e1a) and in flow cytometry on human samples at 1:50 (fig 1a, s3c). Nature (2019) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; mouse; loading ...; fig s6j
Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12) was used in immunohistochemistry - paraffin section on mouse samples (fig s6j). Cell (2019) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; mouse; loading ...; fig 3c
Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12) was used in immunocytochemistry on mouse samples (fig 3c). J Clin Invest (2019) ncbi
mouse monoclonal (F55A12)
  • flow cytometry; human; 1:250; fig 4b
  • immunocytochemistry; human; 1:500; fig 4e
Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12-c) was used in flow cytometry on human samples at 1:250 (fig 4b) and in immunocytochemistry on human samples at 1:500 (fig 4e). Cell Stem Cell (2017) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry; mouse; 1:25; loading ...; fig s2c
In order to study regulation of Notch1 in ventral neural stem/progenitor cells of the developing spinal cord, Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12) was used in immunohistochemistry on mouse samples at 1:25 (fig s2c). Sci Rep (2016) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; human; 1:50; loading ...; tbl 1
In order to describe a method to produce human embryonic stem cell-derived Beta-like cells, Developmental Studies Hybridoma Bank NKX6-1 antibody (Hybridoma Bank, F55A12) was used in immunocytochemistry on human samples at 1:50 (tbl 1). PLoS ONE (2016) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; mouse; fig 6
In order to use a blinded study to examine the effects of ANGPTL8 upon beta-cell proliferation, Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12-c) was used in immunohistochemistry - paraffin section on mouse samples (fig 6). PLoS ONE (2016) ncbi
mouse monoclonal (F55A12)
  • flow cytometry; human; 1:200; fig s19
Developmental Studies Hybridoma Bank NKX6-1 antibody (Developmental Studies Hybridoma Bank, F55A12-s) was used in flow cytometry on human samples at 1:200 (fig s19). Nat Commun (2016) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; human; fig 3
Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12) was used in immunohistochemistry - paraffin section on human samples (fig 3). Nat Med (2016) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - frozen section; zebrafish ; 1:500; fig 3
Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12) was used in immunohistochemistry - frozen section on zebrafish samples at 1:500 (fig 3). Genes Dev (2015) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; mouse
  • western blot; mouse
Developmental Studies Hybridoma Bank NKX6-1 antibody (DHSB, F55A12-c) was used in immunocytochemistry on mouse samples and in western blot on mouse samples . Nat Biotechnol (2014) ncbi
mouse monoclonal (F55A12)
  • immunocytochemistry; human; 1:100; fig 5
In order to describe a scalable, automated approach to test the ability of small molecules to promote human pluripotent stem cell differentiation, Developmental Studies Hybridoma Bank NKX6-1 antibody (DSHB, F55A12) was used in immunocytochemistry on human samples at 1:100 (fig 5). Nat Biotechnol (2015) ncbi
mouse monoclonal (F55A12)
  • immunohistochemistry - paraffin section; mouse; 1:50
In order to investigate the function of islet-1 in the pancreas, Developmental Studies Hybridoma Bank NKX6-1 antibody (Hybridoma Bank, F55A12-C) was used in immunohistochemistry - paraffin section on mouse samples at 1:50. Diabetes (2014) ncbi
Articles Reviewed
  1. Veres A, Faust A, Bushnell H, Engquist E, Kenty J, Harb G, et al. Charting cellular identity during human in vitro β-cell differentiation. Nature. 2019;569:368-373 pubmed publisher
  2. Sharon N, Chawla R, Mueller J, Vanderhooft J, Whitehorn L, Rosenthal B, et al. A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. Cell. 2019;176:790-804.e13 pubmed publisher
  3. Bartolomé A, Zhu C, Sussel L, Pajvani U. Notch signaling dynamically regulates adult β cell proliferation and maturity. J Clin Invest. 2019;129:268-280 pubmed publisher
  4. Shi Z, Lee K, Yang D, Amin S, Verma N, Li Q, et al. Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development. Cell Stem Cell. 2017;20:675-688.e6 pubmed publisher
  5. Li Y, Tzatzalos E, Kwan K, Grumet M, Cai L. Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development. Sci Rep. 2016;6:38665 pubmed publisher
  6. Massumi M, Pourasgari F, Nalla A, Batchuluun B, Nagy K, Neely E, et al. An Abbreviated Protocol for In Vitro Generation of Functional Human Embryonic Stem Cell-Derived Beta-Like Cells. PLoS ONE. 2016;11:e0164457 pubmed publisher
  7. Cox A, Barrandon O, Cai E, Rios J, Chavez J, Bonnyman C, et al. Resolving Discrepant Findings on ANGPTL8 in ?-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversy. PLoS ONE. 2016;11:e0159276 pubmed publisher
  8. Saxena P, Heng B, Bai P, Folcher M, Zulewski H, Fussenegger M. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells. Nat Commun. 2016;7:11247 pubmed publisher
  9. Vegas A, Veiseh O, Gürtler M, Millman J, Pagliuca F, Bader A, et al. Long-term glycemic control using polymer-encapsulated human stem cell-derived beta cells in immune-competent mice. Nat Med. 2016;22:306-11 pubmed publisher
  10. Ravanelli A, Appel B. Motor neurons and oligodendrocytes arise from distinct cell lineages by progenitor recruitment. Genes Dev. 2015;29:2504-15 pubmed publisher
  11. Li W, Cavelti Weder C, Zhang Y, Zhang Y, Clement K, Donovan S, et al. Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells. Nat Biotechnol. 2014;32:1223-30 pubmed publisher
  12. Maury Y, Côme J, Piskorowski R, Salah Mohellibi N, Chevaleyre V, Peschanski M, et al. Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol. 2015;33:89-96 pubmed publisher
  13. Ediger B, Du A, Liu J, Hunter C, Walp E, Schug J, et al. Islet-1 Is essential for pancreatic ?-cell function. Diabetes. 2014;63:4206-17 pubmed publisher