This is a Validated Antibody Database (VAD) review about bovine MFN2, based on 31 published articles (read how Labome selects the articles), using MFN2 antibody in all methods. It is aimed to help Labome visitors find the most suited MFN2 antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Abcam
mouse monoclonal
  • western blot knockout validation; human; 1:500; fig s1b
In order to investigate PINK1-Mfn2-Parkin-mediated mitophagy in mouse hearts, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on human samples at 1:500 (fig s1b). Science (2015) ncbi
Abcam
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig s4
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig s4). J Cell Biol (2015) ncbi
Abcam
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Cell Metab (2015) ncbi
Abcam
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...; fig s7b
Abcam MFN2 antibody (Abcam, Ab56889) was used in western blot on mouse samples at 1:1000 (fig s7b). Cell Death Dis (2021) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...; fig 3a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples at 1:1000 (fig 3a). Front Cell Dev Biol (2021) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 5a
Abcam MFN2 antibody (Abcam, 56889) was used in western blot on mouse samples (fig 5a). Aging Cell (2021) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...; fig 2b
Abcam MFN2 antibody (AbCAM, ab56889) was used in western blot on mouse samples at 1:1000 (fig 2b). elife (2020) ncbi
mouse monoclonal
  • western blot; human; 1:1000; loading ...; fig 3c
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples at 1:1000 (fig 3c). Life Sci Alliance (2020) ncbi
mouse monoclonal
  • western blot; mouse; 1:2000; loading ...; fig 6a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples at 1:2000 (fig 6a). Antioxidants (Basel) (2020) ncbi
mouse monoclonal
  • western blot; human; loading ...; fig 2i
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples (fig 2i). Cell Death Differ (2020) ncbi
mouse monoclonal
  • western blot; mouse; 1:2000; loading ...
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples at 1:2000. Cell Death Dis (2020) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig s1e
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig s1e). Cell (2019) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...; fig 5a
Abcam MFN2 antibody (Abcam, Ab56889) was used in western blot on mouse samples at 1:1000 (fig 5a). Aging Cell (2019) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 4a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig 4a). Cell Rep (2018) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig s1c
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig s1c). Cell Rep (2018) ncbi
mouse monoclonal
  • western blot; human; 1:1000; loading ...; fig 8f
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples at 1:1000 (fig 8f). J Biol Chem (2018) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 3c
Abcam MFN2 antibody (abcam, ab56889) was used in western blot on mouse samples (fig 3c). J Mol Histol (2018) ncbi
mouse monoclonal
  • western blot; mouse; 1:1000; loading ...; fig 2a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples at 1:1000 (fig 2a). Endocrinology (2018) ncbi
mouse monoclonal
  • immunohistochemistry; mouse; loading ...; fig 5e
  • western blot; mouse; loading ...; fig 2a
  • western blot; human; loading ...; fig 5c
Abcam MFN2 antibody (Abcam, 56889) was used in immunohistochemistry on mouse samples (fig 5e), in western blot on mouse samples (fig 2a) and in western blot on human samples (fig 5c). Autophagy (2017) ncbi
mouse monoclonal
  • western blot; human; 1:5000; loading ...; fig 2a
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples at 1:5000 (fig 2a). Brain (2017) ncbi
mouse monoclonal
  • western blot; mouse; loading ...; fig 4b
In order to evaluate an approach for correcting mitochondrial fusion, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig 4b). Nature (2016) ncbi
mouse monoclonal
  • western blot; mouse; 1:2000; fig 6
In order to study affects of acute minimization of brown adipose tissue function by increased reliance on muscle based thermogenesis, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples at 1:2000 (fig 6). J Biol Chem (2016) ncbi
mouse monoclonal
  • western blot; mouse; fig s4
In order to study inhibition of mitochondrial fragmentation and attenuation of kainic acid-induced hippocampal cell death by a mitochondrial division inhibitor, Mdivi-1, Abcam MFN2 antibody (Abcam, 56889) was used in western blot on mouse samples (fig s4). BMC Neurosci (2016) ncbi
mouse monoclonal
  • western blot; human; fig s1
Abcam MFN2 antibody (Abcam, 56889) was used in western blot on human samples (fig s1). J Immunol (2016) ncbi
mouse monoclonal
  • immunocytochemistry; mouse; fig 2
  • western blot; mouse; fig 4
Abcam MFN2 antibody (Abcam, ab56889) was used in immunocytochemistry on mouse samples (fig 2) and in western blot on mouse samples (fig 4). Nature (2016) ncbi
mouse monoclonal
  • western blot knockout validation; human; 1:500; fig s1b
In order to investigate PINK1-Mfn2-Parkin-mediated mitophagy in mouse hearts, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on human samples at 1:500 (fig s1b). Science (2015) ncbi
mouse monoclonal
  • flow cytometry; mouse; fig 1h
In order to develop the MitoTimer protein to study individual mitochondria, Abcam MFN2 antibody (Abcam, ab56889) was used in flow cytometry on mouse samples (fig 1h). J Mol Cell Cardiol (2016) ncbi
mouse monoclonal
  • other; mouse; 1:500; fig s4
Abcam MFN2 antibody (Abcam, ab56889) was used in other on mouse samples at 1:500 (fig s4). Nat Commun (2015) ncbi
mouse monoclonal
  • western blot; rat; fig 6d
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on rat samples (fig 6d). Cell Res (2015) ncbi
mouse monoclonal
  • western blot; human; fig s1
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples (fig s1). Nat Neurosci (2015) ncbi
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig s4
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig s4). J Cell Biol (2015) ncbi
mouse monoclonal
  • western blot knockout validation; mouse; 1:1000; fig 1
Abcam MFN2 antibody (Abcam, ab56889) was used in western blot knockout validation on mouse samples at 1:1000 (fig 1). Cell Metab (2015) ncbi
mouse monoclonal
  • western blot; mouse; fig 3
In order to study how obesity alters mitochondria-associated ER membranes, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on mouse samples (fig 3). Nat Med (2014) ncbi
mouse monoclonal
  • western blot; human; 1:500
In order to report and characterize the interaction between BECN1 and PARK2, Abcam MFN2 antibody (Abcam, ab56889) was used in western blot on human samples at 1:500. Autophagy (2014) ncbi
Articles Reviewed
  1. Park G, Lee J, Han H, An H, Jin Z, Jeong E, et al. Ablation of dynamin-related protein 1 promotes diabetes-induced synaptic injury in the hippocampus. Cell Death Dis. 2021;12:445 pubmed publisher
  2. Tian F, Zhang Y. Overexpression of SERCA2a Alleviates Cardiac Microvascular Ischemic Injury by Suppressing Mfn2-Mediated ER/Mitochondrial Calcium Tethering. Front Cell Dev Biol. 2021;9:636553 pubmed publisher
  3. Niu M, Zhao F, Bondelid K, Siedlak S, Torres S, Fujioka H, et al. VPS35 D620N knockin mice recapitulate cardinal features of Parkinson's disease. Aging Cell. 2021;20:e13347 pubmed publisher
  4. Franco A, Dang X, Walton E, Ho J, Zablocka B, Ly C, et al. Burst mitofusin activation reverses neuromuscular dysfunction in murine CMT2A. elife. 2020;9: pubmed publisher
  5. Rusilowicz Jones E, Jardine J, Kallinos A, Pinto Fernandez A, Guenther F, Giurrandino M, et al. USP30 sets a trigger threshold for PINK1-PARKIN amplification of mitochondrial ubiquitylation. Life Sci Alliance. 2020;3: pubmed publisher
  6. Meza Torres C, Hernández Camacho J, Cortés Rodríguez A, Fang L, Bui Thanh T, Rodríguez Bies E, et al. Resveratrol Regulates the Expression of Genes Involved in CoQ Synthesis in Liver in Mice Fed with High Fat Diet. Antioxidants (Basel). 2020;9: pubmed publisher
  7. Simula L, Corrado M, Accordi B, Di Rita A, Nazio F, Antonucci Y, et al. JNK1 and ERK1/2 modulate lymphocyte homeostasis via BIM and DRP1 upon AICD induction. Cell Death Differ. 2020;: pubmed publisher
  8. Edwards G, Perkins G, Kim K, Kong Y, Lee Y, Choi S, et al. Loss of AKAP1 triggers Drp1 dephosphorylation-mediated mitochondrial fission and loss in retinal ganglion cells. Cell Death Dis. 2020;11:254 pubmed publisher
  9. Hernández Alvarez M, Sebastian D, Vives S, Ivanova S, Bartoccioni P, Kakimoto P, et al. Deficient Endoplasmic Reticulum-Mitochondrial Phosphatidylserine Transfer Causes Liver Disease. Cell. 2019;177:881-895.e17 pubmed publisher
  10. Song C, Zhang J, Qi S, Liu Z, Zhang X, Zheng Y, et al. Cardiolipin remodeling by ALCAT1 links mitochondrial dysfunction to Parkinson's diseases. Aging Cell. 2019;18:e12941 pubmed publisher
  11. D Eletto M, Rossin F, Occhigrossi L, Farrace M, Faccenda D, Desai R, et al. Transglutaminase Type 2 Regulates ER-Mitochondria Contact Sites by Interacting with GRP75. Cell Rep. 2018;25:3573-3581.e4 pubmed publisher
  12. Simula L, Pacella I, Colamatteo A, Procaccini C, Cancila V, Bordi M, et al. Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming. Cell Rep. 2018;25:3059-3073.e10 pubmed publisher
  13. De R, Sarkar S, Mazumder S, Debsharma S, Siddiqui A, Saha S, et al. Macrophage migration inhibitory factor regulates mitochondrial dynamics and cell growth of human cancer cell lines through CD74-NF-κB signaling. J Biol Chem. 2018;293:19740-19760 pubmed publisher
  14. Zhao H, Pan W, Chen L, Luo Y, Xu R. Nur77 promotes cerebral ischemia-reperfusion injury via activating INF2-mediated mitochondrial fragmentation. J Mol Histol. 2018;49:599-613 pubmed publisher
  15. Viana Huete V, Guillen C, García G, Fernandez S, García Aguilar A, Kahn C, et al. Male Brown Fat-Specific Double Knockout of IGFIR/IR: Atrophy, Mitochondrial Fission Failure, Impaired Thermogenesis, and Obesity. Endocrinology. 2018;159:323-340 pubmed publisher
  16. Wang Q, Wu S, Zhu H, Ding Y, Dai X, Ouyang C, et al. Deletion of PRKAA triggers mitochondrial fission by inhibiting the autophagy-dependent degradation of DNM1L. Autophagy. 2017;13:404-422 pubmed publisher
  17. Puschmann A, Fiesel F, Caulfield T, Hudec R, Ando M, Truban D, et al. Heterozygous PINK1 p.G411S increases risk of Parkinson's disease via a dominant-negative mechanism. Brain. 2017;140:98-117 pubmed publisher
  18. Franco A, Kitsis R, Fleischer J, Gavathiotis E, Kornfeld O, Gong G, et al. Correcting mitochondrial fusion by manipulating mitofusin conformations. Nature. 2016;540:74-79 pubmed publisher
  19. Bal N, Maurya S, Singh S, Wehrens X, Periasamy M. Increased Reliance on Muscle-based Thermogenesis upon Acute Minimization of Brown Adipose Tissue Function. J Biol Chem. 2016;291:17247-57 pubmed publisher
  20. Kim H, Lee J, Park K, Kim W, Roh G. A mitochondrial division inhibitor, Mdivi-1, inhibits mitochondrial fragmentation and attenuates kainic acid-induced hippocampal cell death. BMC Neurosci. 2016;17:33 pubmed publisher
  21. Kobayashi K, Araya J, Minagawa S, Hara H, Saito N, Kadota T, et al. Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis. J Immunol. 2016;197:504-16 pubmed publisher
  22. Luchsinger L, de Almeida M, Corrigan D, Mumau M, Snoeck H. Mitofusin 2 maintains haematopoietic stem cells with extensive lymphoid potential. Nature. 2016;529:528-31 pubmed publisher
  23. Gong G, Song M, Csordás G, Kelly D, Matkovich S, Dorn G. Parkin-mediated mitophagy directs perinatal cardiac metabolic maturation in mice. Science. 2015;350:aad2459 pubmed publisher
  24. Stotland A, Gottlieb R. α-MHC MitoTimer mouse: In vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart. J Mol Cell Cardiol. 2016;90:53-8 pubmed publisher
  25. Wu S, Kao C, Wang L, Creighton C, Yang J, Donti T, et al. Increased COUP-TFII expression in adult hearts induces mitochondrial dysfunction resulting in heart failure. Nat Commun. 2015;6:8245 pubmed publisher
  26. Wang C, Du W, Su Q, Zhu M, Feng P, Li Y, et al. Dynamic tubulation of mitochondria drives mitochondrial network formation. Cell Res. 2015;25:1108-20 pubmed publisher
  27. Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, et al. Mitochondrial control by DRP1 in brain tumor initiating cells. Nat Neurosci. 2015;18:501-10 pubmed publisher
  28. Mourier A, Motori E, Brandt T, Lagouge M, Atanassov I, Galinier A, et al. Mitofusin 2 is required to maintain mitochondrial coenzyme Q levels. J Cell Biol. 2015;208:429-42 pubmed publisher
  29. Song M, Mihara K, Chen Y, Scorrano L, Dorn G. Mitochondrial fission and fusion factors reciprocally orchestrate mitophagic culling in mouse hearts and cultured fibroblasts. Cell Metab. 2015;21:273-85 pubmed publisher
  30. Arruda A, Pers B, Parlakgül G, Güney E, Inouye K, Hotamisligil G. Chronic enrichment of hepatic endoplasmic reticulum-mitochondria contact leads to mitochondrial dysfunction in obesity. Nat Med. 2014;20:1427-35 pubmed publisher
  31. Choubey V, Cagalinec M, Liiv J, Safiulina D, Hickey M, Kuum M, et al. BECN1 is involved in the initiation of mitophagy: it facilitates PARK2 translocation to mitochondria. Autophagy. 2014;10:1105-19 pubmed publisher