This is a Validated Antibody Database (VAD) review about bovine ILK, based on 14 published articles (read how Labome selects the articles), using ILK antibody in all methods. It is aimed to help Labome visitors find the most suited ILK antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
Knockout validation
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a, 1b
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1c, 1d
Cell Signaling Technology ILK antibody (Cell Signaling, 3862) was used in western blot on human samples at 1:1000 (fig 1a, 1b) and in western blot knockout validation on mouse samples at 1:1000 (fig 1c, 1d). Mol Metab (2021) ncbi
Cell Signaling Technology
domestic rabbit monoclonal (4G9)
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1c
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1c
  • western blot; mouse; 1:1000; loading ...; fig 9a
Cell Signaling Technology ILK antibody (Cell Signaling, 3856) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1c), in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1c) and in western blot on mouse samples at 1:1000 (fig 9a). J Neurosci (2017) ncbi
Abcam
domestic rabbit monoclonal (EP1593Y)
  • western blot; human; 1:100; fig s7
In order to analyze organotropic metasistasis and tumour exosome integrins, Abcam ILK antibody (Abcam, ab52480) was used in western blot on human samples at 1:100 (fig s7). Nature (2015) ncbi
domestic rabbit monoclonal (EP1593Y)
  • western blot; rat; fig 1
  • western blot; mouse; fig 2a
In order to generate and characterize PINCH 1 and PINCH 2 double knockout mice, Abcam ILK antibody (abcam, ab52480) was used in western blot on rat samples (fig 1) and in western blot on mouse samples (fig 2a). PLoS ONE (2013) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1a, 1b
  • western blot knockout validation; mouse; 1:1000; loading ...; fig 1c, 1d
Cell Signaling Technology ILK antibody (Cell Signaling, 3862) was used in western blot on human samples at 1:1000 (fig 1a, 1b) and in western blot knockout validation on mouse samples at 1:1000 (fig 1c, 1d). Mol Metab (2021) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 8f
Cell Signaling Technology ILK antibody (Cell Signaling, 3862) was used in western blot on human samples (fig 8f). EMBO J (2019) ncbi
domestic rabbit monoclonal (4G9)
  • western blot; human; loading ...; fig 3i
Cell Signaling Technology ILK antibody (Cell Signaling, 3856) was used in western blot on human samples (fig 3i). Sci Rep (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; fig 6a
In order to see if tetraspanin 8 influences cell-matrix anchorage and regulates downstream molecular pathways leading to aggressive behavior of tumor progression in melanoma, Cell Signaling Technology ILK antibody (Cell Signaling, 3862) was used in western blot on human samples (fig 6a). Oncotarget (2017) ncbi
domestic rabbit monoclonal (4G9)
  • immunohistochemistry knockout validation; mouse; 1:500; loading ...; fig 1c
  • immunohistochemistry - frozen section; mouse; 1:500; loading ...; fig 1c
  • western blot; mouse; 1:1000; loading ...; fig 9a
Cell Signaling Technology ILK antibody (Cell Signaling, 3856) was used in immunohistochemistry knockout validation on mouse samples at 1:500 (fig 1c), in immunohistochemistry - frozen section on mouse samples at 1:500 (fig 1c) and in western blot on mouse samples at 1:1000 (fig 9a). J Neurosci (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 5a
In order to test if OSU-T315 inhibits vestibular schwannoma and meningioma cell growth, Cell Signaling Technology ILK antibody (Cell Signaling, 3862) was used in western blot on human samples at 1:1000 (fig 5a). Austin J Med Oncol (2016) ncbi
domestic rabbit polyclonal
  • western blot; mouse; fig 1
In order to learn about the control of differentiation in mammary epithelial cells by the integrin-mediated ILK-parvin-alpha-Pix signaling axis, Cell Signaling Technology ILK antibody (Cell Signaling, 3862) was used in western blot on mouse samples (fig 1). J Cell Physiol (2016) ncbi
domestic rabbit monoclonal (4G9)
  • immunocytochemistry; human; loading ...; fig 2a
  • western blot; human; loading ...; fig 2c
Cell Signaling Technology ILK antibody (Cell signaling, 3856) was used in immunocytochemistry on human samples (fig 2a) and in western blot on human samples (fig 2c). Oncotarget (2015) ncbi
MilliporeSigma
mouse monoclonal (65.1)
  • western blot; mouse; 1:2000; fig s16
MilliporeSigma ILK antibody (Sigma, 65.1) was used in western blot on mouse samples at 1:2000 (fig s16). Nat Commun (2016) ncbi
mouse monoclonal (65.1)
  • western blot; mouse; 1:1000; fig 5
MilliporeSigma ILK antibody (Cell Signaling, I0783) was used in western blot on mouse samples at 1:1000 (fig 5). Nat Commun (2015) ncbi
mouse monoclonal (65.1)
  • immunocytochemistry; human; 1:800; fig 2
MilliporeSigma ILK antibody (Sigma, 65.1) was used in immunocytochemistry on human samples at 1:800 (fig 2). Sci Rep (2015) ncbi
mouse monoclonal (65.1)
  • immunohistochemistry; mouse; fig s1
MilliporeSigma ILK antibody (Sigma, I0783) was used in immunohistochemistry on mouse samples (fig s1). BMC Biol (2013) ncbi
Articles Reviewed
  1. Bugler Lamb A, Hasib A, Weng X, Hennayake C, Lin C, McCrimmon R, et al. Adipocyte integrin-linked kinase plays a key role in the development of diet-induced adipose insulin resistance in male mice. Mol Metab. 2021;49:101197 pubmed publisher
  2. Urner S, Planas Paz L, Hilger L, Henning C, Branopolski A, Kelly Goss M, et al. Identification of ILK as a critical regulator of VEGFR3 signalling and lymphatic vascular growth. EMBO J. 2019;38: pubmed publisher
  3. Merhi A, Delree P, Marini A. The metabolic waste ammonium regulates mTORC2 and mTORC1 signaling. Sci Rep. 2017;7:44602 pubmed publisher
  4. El Kharbili M, Robert C, Witkowski T, Danty Berger E, Barbollat Boutrand L, Masse I, et al. Tetraspanin 8 is a novel regulator of ILK-driven ?1 integrin adhesion and signaling in invasive melanoma cells. Oncotarget. 2017;8:17140-17155 pubmed publisher
  5. Hussain R, Macklin W. Integrin-Linked Kinase (ILK) Deletion Disrupts Oligodendrocyte Development by Altering Cell Cycle. J Neurosci. 2017;37:397-412 pubmed publisher
  6. Mercado Pimentel M, Igarashi S, Dunn A, Behbahani M, Miller C, Read C, et al. The Novel Small Molecule Inhibitor, OSU-T315, Suppresses Vestibular Schwannoma and Meningioma Growth by Inhibiting PDK2 Function in the AKT Pathway Activation. Austin J Med Oncol. 2016;3: pubmed
  7. Zhang C, Wang P, Liu D, Chen C, Zhao W, Chen X, et al. The molecular basis of the genesis of basal tone in internal anal sphincter. Nat Commun. 2016;7:11358 pubmed publisher
  8. Rooney N, Wang P, Brennan K, Gilmore A, Streuli C. The Integrin-Mediated ILK-Parvin-?Pix Signaling Axis Controls Differentiation in Mammary Epithelial Cells. J Cell Physiol. 2016;231:2408-17 pubmed publisher
  9. Hoshino A, Costa Silva B, Shen T, Rodrigues G, Hashimoto A, Tesic Mark M, et al. Tumour exosome integrins determine organotropic metastasis. Nature. 2015;527:329-35 pubmed publisher
  10. Hausmann C, Temme A, Cordes N, Eke I. ILKAP, ILK and PINCH1 control cell survival of p53-wildtype glioblastoma cells after irradiation. Oncotarget. 2015;6:34592-605 pubmed publisher
  11. Wu C, Jiao H, Lai Y, Zheng W, Chen K, Qu H, et al. Kindlin-2 controls TGF-β signalling and Sox9 expression to regulate chondrogenesis. Nat Commun. 2015;6:7531 pubmed publisher
  12. Morris E, Assi K, Salh B, Dedhar S. Integrin-linked kinase links dynactin-1/dynactin-2 with cortical integrin receptors to orient the mitotic spindle relative to the substratum. Sci Rep. 2015;5:8389 pubmed publisher
  13. Dai X, Jiang W, Zhang Q, Xu L, Geng P, Zhuang S, et al. Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis. BMC Biol. 2013;11:107 pubmed publisher
  14. Donthamsetty S, Bhave V, Mars W, Bowen W, Orr A, Haynes M, et al. Role of PINCH and its partner tumor suppressor Rsu-1 in regulating liver size and tumorigenesis. PLoS ONE. 2013;8:e74625 pubmed publisher