This is a Validated Antibody Database (VAD) review about bovine HSPA1A, based on 51 published articles (read how Labome selects the articles), using HSPA1A antibody in all methods. It is aimed to help Labome visitors find the most suited HSPA1A antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
HSPA1A synonym: HSP70; HSP70-1; HSP70-2; HSPA1; HSPA1B; HSPA2

Knockout validation
Abcam
mouse monoclonal (C92F3A-5)
  • western blot knockout validation; human; 1:5000; loading ...; fig 1b
Abcam HSPA1A antibody (Abcam, ab47454) was used in western blot knockout validation on human samples at 1:5000 (fig 1b). elife (2020) ncbi
StressMarq Biosciences
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:3000; fig 1
  • western blot knockout validation; mouse; 1:3000; fig 2
StressMarq Biosciences HSPA1A antibody (StressMarq, SMC-100B) was used in western blot on human samples at 1:3000 (fig 1) and in western blot knockout validation on mouse samples at 1:3000 (fig 2). ASN Neuro (2015) ncbi
Abcam
mouse monoclonal (5A5)
  • western blot; mouse; 1:1000; loading ...; fig 2c
Abcam HSPA1A antibody (Abcam, ab2787) was used in western blot on mouse samples at 1:1000 (fig 2c). Acta Neuropathol Commun (2020) ncbi
mouse monoclonal (C92F3A-5)
  • western blot knockout validation; human; 1:5000; loading ...; fig 1b
Abcam HSPA1A antibody (Abcam, ab47454) was used in western blot knockout validation on human samples at 1:5000 (fig 1b). elife (2020) ncbi
mouse monoclonal (5A5)
  • western blot; rat; 1:800; loading ...; fig 4a
Abcam HSPA1A antibody (Abcam, ab2787) was used in western blot on rat samples at 1:800 (fig 4a). Aging (Albany NY) (2020) ncbi
mouse monoclonal (C92F3A-5)
  • immunohistochemistry - paraffin section; human; 1:100; loading ...; fig 1f
  • immunocytochemistry; human; 1:100; loading ...; fig 2i
Abcam HSPA1A antibody (Abcam, ab47455) was used in immunohistochemistry - paraffin section on human samples at 1:100 (fig 1f) and in immunocytochemistry on human samples at 1:100 (fig 2i). Nat Cell Biol (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1:1000; loading ...; fig 1i
Abcam HSPA1A antibody (Abcam, ab31010) was used in western blot on human samples at 1:1000 (fig 1i). Aging (Albany NY) (2019) ncbi
mouse monoclonal (3A3)
  • western blot; human; loading ...; fig s2j
Abcam HSPA1A antibody (Abcam, ab5439) was used in western blot on human samples (fig s2j). Cell (2019) ncbi
mouse monoclonal (5A5)
  • western blot; human; loading ...; fig s2
Abcam HSPA1A antibody (Abcam, ab2787) was used in western blot on human samples (fig s2). BMC Cancer (2019) ncbi
mouse monoclonal (3A3)
  • western blot; brewer's yeast; 1:1000; fig 4c
Abcam HSPA1A antibody (Abcam, ab5439) was used in western blot on brewer's yeast samples at 1:1000 (fig 4c). elife (2019) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; mouse; 1:1000; loading ...; fig 3b
Abcam HSPA1A antibody (Abcam, ab47455) was used in western blot on mouse samples at 1:1000 (fig 3b). Stress (2019) ncbi
mouse monoclonal (5A5)
  • western blot; human; 1:1000; loading ...; fig 6d
Abcam HSPA1A antibody (Abcam, ab2787) was used in western blot on human samples at 1:1000 (fig 6d). EMBO Mol Med (2019) ncbi
mouse monoclonal (3A3)
  • immunocytochemistry; human; loading ...; fig 6b
Abcam HSPA1A antibody (Abcam, ab5439) was used in immunocytochemistry on human samples (fig 6b). EMBO J (2018) ncbi
mouse monoclonal (5A5)
  • western blot; mouse; fig 5e
Abcam HSPA1A antibody (Abcam, ab2787) was used in western blot on mouse samples (fig 5e). J Lipid Res (2017) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; loading ...; fig s3b
In order to study the suppressive effect of DNAJB6 and Hsp70 on alpha-synuclein aggregation, Abcam HSPA1A antibody (Abcam, ab47455) was used in western blot on human samples (fig s3b). Sci Rep (2017) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:1000; fig 1
In order to research differentiation of functional glutamatergic neurons from placenta-derived multipotent cells by knocking down of heat-shock protein 27, Abcam HSPA1A antibody (Abcam, ab47455) was used in western blot on human samples at 1:1000 (fig 1). Sci Rep (2016) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; rat; 1:1000; loading ...; fig 3b
Abcam HSPA1A antibody (Abcam, ab47455) was used in western blot on rat samples at 1:1000 (fig 3b). In Vitro Cell Dev Biol Anim (2016) ncbi
mouse monoclonal (3A3)
  • immunohistochemistry - frozen section; human; fig 5
In order to characterize dermatomyositis with or without anti-melanoma differentiation-associated gene 5 antibodies that show distinct NOS2 expression but a common interferon signature, Abcam HSPA1A antibody (Abcam, 3A3) was used in immunohistochemistry - frozen section on human samples (fig 5). Am J Pathol (2016) ncbi
mouse monoclonal (3A3)
  • immunohistochemistry; guinea pig; fig 2
Abcam HSPA1A antibody (Abcam, ab5439) was used in immunohistochemistry on guinea pig samples (fig 2). Cell Death Dis (2016) ncbi
mouse monoclonal (3A3)
  • western blot; human; fig 2
Abcam HSPA1A antibody (Abcam, ab5439) was used in western blot on human samples (fig 2). Sci Rep (2016) ncbi
mouse monoclonal (C92F3A-5)
  • immunoprecipitation; human; loading ...; fig 2a
  • immunocytochemistry; human; loading ...; fig 3d
  • western blot; human; loading ...; fig 3c
Abcam HSPA1A antibody (Abcam, ab47455) was used in immunoprecipitation on human samples (fig 2a), in immunocytochemistry on human samples (fig 3d) and in western blot on human samples (fig 3c). FASEB J (2016) ncbi
domestic rabbit polyclonal
  • immunocytochemistry; mouse; 1:100
In order to show that that both cell-mediated and humoral adaptive immune components are involved in photodynamic therapy, Abcam HSPA1A antibody (Abcam, ab31010) was used in immunocytochemistry on mouse samples at 1:100. Cell Mol Immunol (2017) ncbi
mouse monoclonal (5A5)
  • western blot; pigs ; loading ...; fig 6c
Abcam HSPA1A antibody (Abcam, ab2787) was used in western blot on pigs samples (fig 6c). PLoS ONE (2015) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; African green monkey
In order to perform a proteomic analysis of host proteins in Rift Valley fever virus virions and to study the particular significance of Hsp90, Abcam HSPA1A antibody (Abcam, ab47455) was used in western blot on African green monkey samples . PLoS ONE (2014) ncbi
mouse monoclonal (3A3)
  • immunohistochemistry - frozen section; rat; 1:50
  • western blot; rat; 1:1000
Abcam HSPA1A antibody (Abcam, ab5439) was used in immunohistochemistry - frozen section on rat samples at 1:50 and in western blot on rat samples at 1:1000. Theranostics (2013) ncbi
mouse monoclonal (5A5)
  • western blot; mouse
Abcam HSPA1A antibody (Abcam, ab2787) was used in western blot on mouse samples . Proteomics (2014) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; fig s3b
Abcam HSPA1A antibody (Abcam, ab47455) was used in western blot on human samples (fig s3b). PLoS ONE (2012) ncbi
Santa Cruz Biotechnology
mouse monoclonal (C92F3A-5)
  • western blot; human; loading ...; fig 2d, 4e
Santa Cruz Biotechnology HSPA1A antibody (Santa Cruz, sc-66048) was used in western blot on human samples (fig 2d, 4e). iScience (2021) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; mouse; loading ...; fig 4d
  • western blot; human; fig 7a
Santa Cruz Biotechnology HSPA1A antibody (Santa Cruz, sc-66048) was used in western blot on mouse samples (fig 4d) and in western blot on human samples (fig 7a). J Clin Invest (2020) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:1000; loading ...; fig ex1m
Santa Cruz Biotechnology HSPA1A antibody (Santa, sc-66048) was used in western blot on human samples at 1:1000 (fig ex1m). Nature (2018) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; mouse; 1:200; fig 3
Santa Cruz Biotechnology HSPA1A antibody (Santa Cruz, SC-66048) was used in western blot on mouse samples at 1:200 (fig 3). Front Microbiol (2016) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; mouse; 1:1000; fig 8
Santa Cruz Biotechnology HSPA1A antibody (Santa Cruz Biotechnologies, C92F3A-5) was used in western blot on mouse samples at 1:1000 (fig 8). Sci Rep (2016) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; mouse; fig 1, 2
In order to demonstrate that p38MAPK activation elevates mitochondrial ROS levels, Santa Cruz Biotechnology HSPA1A antibody (Santa Cruz Biotechnology, sc-66048) was used in western blot on mouse samples (fig 1, 2). Cell Commun Signal (2014) ncbi
StressMarq Biosciences
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:1000; loading ...; fig 6h, 2g, 6s1e
StressMarq Biosciences HSPA1A antibody (StressMarq, C92F3A-5) was used in western blot on human samples at 1:1000 (fig 6h, 2g, 6s1e). elife (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6a
StressMarq Biosciences HSPA1A antibody (StressMarq Biosciences, SPC-103D) was used in western blot on human samples (fig 6a). Autophagy (2017) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:3000; fig 1
  • western blot knockout validation; mouse; 1:3000; fig 2
StressMarq Biosciences HSPA1A antibody (StressMarq, SMC-100B) was used in western blot on human samples at 1:3000 (fig 1) and in western blot knockout validation on mouse samples at 1:3000 (fig 2). ASN Neuro (2015) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; rat
StressMarq Biosciences HSPA1A antibody (StressMarq Biosciences Inc, SMC-100B) was used in western blot on rat samples . PLoS ONE (2014) ncbi
mouse monoclonal (C92F3A-5)
  • western blot; human; 1:1000; fig 6
StressMarq Biosciences HSPA1A antibody (StressMarq, SMC-100) was used in western blot on human samples at 1:1000 (fig 6). Cell Death Dis (2013) ncbi
Invitrogen
mouse monoclonal (W27)
  • western blot; human; fig 2
In order to investigate ANP32C-Hsp90 interactions, Invitrogen HSPA1A antibody (Thermo Scientific, MA1-90504) was used in western blot on human samples (fig 2). Biochim Biophys Acta (2015) ncbi
mouse monoclonal (W27)
  • western blot; human; fig 3
In order to use a cell culture model for aromatase inhibitor-resistant breast cancer to show how fulvestrant treatment and cross-resistance between exemestane and letrozole shows sensitivity, Invitrogen HSPA1A antibody (Neomarkers, MS-482-PO) was used in western blot on human samples (fig 3). Int J Oncol (2015) ncbi
Rockland Immunochemicals
mouse monoclonal (C92F3A-5)
  • western blot; human; fig 4
Rockland Immunochemicals HSPA1A antibody (Rockland, 200-301-A27) was used in western blot on human samples (fig 4). ASN Neuro (2015) ncbi
Cell Signaling Technology
domestic rabbit polyclonal
  • western blot; human; 1:500; loading ...; fig s3b
Cell Signaling Technology HSPA1A antibody (CST, 4872) was used in western blot on human samples at 1:500 (fig s3b). Nat Commun (2020) ncbi
domestic rabbit polyclonal
  • western blot; mouse; loading ...; fig s1c
Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872) was used in western blot on mouse samples (fig s1c). Sci Adv (2019) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 6g
Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872) was used in western blot on human samples (fig 6g). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; fig s5e
Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872) was used in western blot on human samples (fig s5e). Cell (2018) ncbi
domestic rabbit polyclonal
  • other; human; loading ...; fig 4c
Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872) was used in other on human samples (fig 4c). Cancer Cell (2018) ncbi
domestic rabbit polyclonal
  • western blot; rat; loading ...; fig 4b
Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872) was used in western blot on rat samples (fig 4b). J Biol Chem (2018) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig s2b
  • immunohistochemistry; mouse; loading ...; fig s3c
  • western blot; mouse; loading ...; fig s3a
In order to investigate the involvement of HspBP1 in the neuronal susceptibility towards misfolded proteins, Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872S) was used in western blot on human samples (fig s2b), in immunohistochemistry on mouse samples (fig s3c) and in western blot on mouse samples (fig s3a). Proc Natl Acad Sci U S A (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 3b, 3c
Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872) was used in western blot on human samples (fig 3b, 3c). Mol Cell (2017) ncbi
domestic rabbit polyclonal
  • reverse phase protein lysate microarray; human; loading ...; fig st6
In order to characterize the molecular identity of uterine carcinosarcomas., Cell Signaling Technology HSPA1A antibody (CST, 4872) was used in reverse phase protein lysate microarray on human samples (fig st6). Cancer Cell (2017) ncbi
domestic rabbit polyclonal
  • western blot; human; loading ...; fig 2a
Cell Signaling Technology HSPA1A antibody (Cell Signalling, 4872) was used in western blot on human samples (fig 2a). Oncotarget (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; rat; loading ...; fig 4a
  • western blot; rat; 1:1000; loading ...; fig 1a
Cell Signaling Technology HSPA1A antibody (Cell Signaling, 4872S) was used in immunohistochemistry - paraffin section on rat samples (fig 4a) and in western blot on rat samples at 1:1000 (fig 1a). Mol Med Rep (2016) ncbi
domestic rabbit polyclonal
  • flow cytometry; human; 1:25; fig 1b
  • immunohistochemistry; human; loading ...; fig 1a
  • western blot; human; 1:500; fig 1d
In order to assess the effect of shepherdin, a peptido-mimetic that inhibits the heat shock protein-Survivin interaction, in retinoblastoma, Cell Signaling Technology HSPA1A antibody (Cell signaling, 4872) was used in flow cytometry on human samples at 1:25 (fig 1b), in immunohistochemistry on human samples (fig 1a) and in western blot on human samples at 1:500 (fig 1d). Chem Biol Interact (2016) ncbi
Articles Reviewed
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  25. Gupta A, Anjomani Virmouni S, Koundouros N, Dimitriadi M, Choo Wing R, Valle A, et al. PARK2 Depletion Connects Energy and Oxidative Stress to PI3K/Akt Activation via PTEN S-Nitrosylation. Mol Cell. 2017;65:999-1013.e7 pubmed publisher
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  44. Hole S, Pedersen A, Hansen S, Lundqvist J, Yde C, Lykkesfeldt A. New cell culture model for aromatase inhibitor-resistant breast cancer shows sensitivity to fulvestrant treatment and cross-resistance between letrozole and exemestane. Int J Oncol. 2015;46:1481-90 pubmed publisher
  45. Nuss J, Kehn Hall K, Benedict A, Costantino J, Ward M, Peyser B, et al. Multi-faceted proteomic characterization of host protein complement of Rift Valley fever virus virions and identification of specific heat shock proteins, including HSP90, as important viral host factors. PLoS ONE. 2014;9:e93483 pubmed publisher
  46. Akkad H, Corpeno R, Larsson L. Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model. PLoS ONE. 2014;9:e92622 pubmed publisher
  47. Ashraf M, Ebner M, Wallner C, Haller M, Khalid S, Schwelberger H, et al. A p38MAPK/MK2 signaling pathway leading to redox stress, cell death and ischemia/reperfusion injury. Cell Commun Signal. 2014;12:6 pubmed publisher
  48. Agulla J, Brea D, Campos F, Sobrino T, Argibay B, Al Soufi W, et al. In vivo theranostics at the peri-infarct region in cerebral ischemia. Theranostics. 2013;4:90-105 pubmed publisher
  49. Jockusch H, Holland A, Staunton L, Schmitt John T, Heimann P, Dowling P, et al. Pathoproteomics of testicular tissue deficient in the GARP component VPS54: the wobbler mouse model of globozoospermia. Proteomics. 2014;14:839-52 pubmed publisher
  50. Bauckman K, Haller E, Flores I, Nanjundan M. Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells. Cell Death Dis. 2013;4:e592 pubmed publisher
  51. Qi L, Zhang X, Wu J, Lin F, Wang J, DiFiglia M, et al. The role of chaperone-mediated autophagy in huntingtin degradation. PLoS ONE. 2012;7:e46834 pubmed publisher