This is a Validated Antibody Database (VAD) review about bovine H2AX, based on 36 published articles (read how Labome selects the articles), using H2AX antibody in all methods. It is aimed to help Labome visitors find the most suited H2AX antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
H2AX synonym: H2AFX

Abcam
mouse monoclonal (9F3)
  • immunohistochemistry - paraffin section; mouse; fig 4a
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry - paraffin section on mouse samples (fig 4a). Oncotarget (2022) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry; mouse; 1:200; loading ...; fig 2a
  • immunocytochemistry; human; 1:1000; fig 3d
  • immunohistochemistry; human; 1:200; loading ...; fig 1a
  • western blot; human; fig 3c
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry on mouse samples at 1:200 (fig 2a), in immunocytochemistry on human samples at 1:1000 (fig 3d), in immunohistochemistry on human samples at 1:200 (fig 1a) and in western blot on human samples (fig 3c). Cells (2021) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; loading ...; fig 6g
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples (fig 6g). Commun Biol (2021) ncbi
mouse monoclonal (9F3)
  • western blot; mouse; 1:1000; loading ...; fig 7a
  • western blot; human; 1:1000; loading ...; fig s6a
Abcam H2AX antibody (Abcam, ab26350) was used in western blot on mouse samples at 1:1000 (fig 7a) and in western blot on human samples at 1:1000 (fig s6a). Redox Biol (2021) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; 1:250; loading ...; fig 2a
  • immunohistochemistry; human; loading ...; fig 5c, 5d
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples at 1:250 (fig 2a) and in immunohistochemistry on human samples (fig 5c, 5d). J Exp Clin Cancer Res (2021) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; 1:200; loading ...; fig 2a, 2c
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples at 1:200 (fig 2a, 2c). Front Cell Dev Biol (2020) ncbi
mouse monoclonal (9F3)
  • western blot; human; 1:500; fig 2g
Abcam H2AX antibody (Abcam, ab26350) was used in western blot on human samples at 1:500 (fig 2g). Mol Med Rep (2021) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry; human; loading ...; fig 1c
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry on human samples (fig 1c). Oncol Rep (2020) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; fig 7a
Abcam H2AX antibody (Abcam, Ab26350) was used in immunocytochemistry on mouse samples (fig 7a). Vaccines (Basel) (2020) ncbi
mouse monoclonal (9F3)
  • western blot; mouse; loading ...; fig 3e
Abcam H2AX antibody (Abcam, ab26350) was used in western blot on mouse samples (fig 3e). elife (2020) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; loading ...; fig s8c
  • western blot; mouse; 1:5000; fig s8a
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples (fig s8c) and in western blot on mouse samples at 1:5000 (fig s8a). MBio (2020) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; 1:500; loading ...; fig 3b
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples at 1:500 (fig 3b). Nucleic Acids Res (2020) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; 1:500-1:1000; loading ...; fig 6c
  • western blot; human; 1:500-1:2000; loading ...; fig 6a
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples at 1:500-1:1000 (fig 6c) and in western blot on human samples at 1:500-1:2000 (fig 6a). Cell Rep (2019) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2c
  • immunocytochemistry; mouse; loading ...; fig 2f
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry - frozen section on mouse samples (fig 2c) and in immunocytochemistry on mouse samples (fig 2f). elife (2019) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; rat; 1:500; loading ...; fig 3a
Abcam H2AX antibody (ABCAM, ab26350) was used in immunocytochemistry on rat samples at 1:500 (fig 3a). Aging (Albany NY) (2019) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; 1:1000; loading ...; fig 7s1b
  • immunohistochemistry; mouse; 1:1000; loading ...; fig 7a
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples at 1:1000 (fig 7s1b) and in immunohistochemistry on mouse samples at 1:1000 (fig 7a). elife (2019) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; 1:500; loading ...; fig 4c
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples at 1:500 (fig 4c). Sci Rep (2019) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry; mouse; 1:100; loading ...; fig 6a
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry on mouse samples at 1:100 (fig 6a). Biochimie (2018) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; fig 6e
  • immunocytochemistry; human; loading ...; fig 2a, 4f
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples (fig 6e) and in immunocytochemistry on human samples (fig 2a, 4f). Nucleic Acids Res (2018) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; loading ...; fig 1a
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples (fig 1a). Nature (2017) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; 1:500; loading ...; fig 5a
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples at 1:500 (fig 5a). Redox Biol (2017) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; loading ...; fig s20
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples (fig s20). Nucleic Acids Res (2017) ncbi
mouse monoclonal (9F3)
  • western blot; dogs; 1:5000; loading ...; fig 4
In order to report the effect of enrofloxacin addition to either doxorubicin or carboplatin on osteosarcoma cell viability and examine p53 status, Abcam H2AX antibody (Abcam, 26350) was used in western blot on dogs samples at 1:5000 (fig 4). Vet Comp Oncol (2017) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; loading ...; fig 1b
  • western blot; human; loading ...; fig 1a
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples (fig 1b) and in western blot on human samples (fig 1a). Cell Death Differ (2016) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; fig 3
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on human samples (fig 3). Nat Commun (2015) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; 1:400
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples at 1:400. Neurobiol Aging (2015) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; dogs; 1:500; fig 6
  • western blot; dogs; 1:1000; fig 5
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on dogs samples at 1:500 (fig 6) and in western blot on dogs samples at 1:1000 (fig 5). BMC Cancer (2015) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; human; 1:100; loading ...; fig s1
Abcam H2AX antibody (Abcam, 26350) was used in immunocytochemistry on human samples at 1:100 (fig s1). Sci Rep (2015) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry - paraffin section; rat; 1:100
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry - paraffin section on rat samples at 1:100. Reprod Toxicol (2015) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry - paraffin section; rhesus macaque; 1:250; loading ...; fig 3a
Abcam H2AX antibody (Abcam, 9F3) was used in immunohistochemistry - paraffin section on rhesus macaque samples at 1:250 (fig 3a). J Immunol (2015) ncbi
mouse monoclonal (9F3)
  • western blot; dogs
Abcam H2AX antibody (Abcam, ab26350) was used in western blot on dogs samples . Mol Biosyst (2014) ncbi
mouse monoclonal (9F3)
  • immunocytochemistry; mouse; 1:100
Abcam H2AX antibody (Abcam, ab26350) was used in immunocytochemistry on mouse samples at 1:100. Biol Reprod (2014) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry; mouse; 1:200
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry on mouse samples at 1:200. Mol Cell (2013) ncbi
mouse monoclonal (9F3)
  • immunohistochemistry - frozen section; chicken; 1:500
Abcam H2AX antibody (Abcam, ab26350) was used in immunohistochemistry - frozen section on chicken samples at 1:500. Mol Cell Endocrinol (2013) ncbi
Novus Biologicals
mouse monoclonal (3F2)
  • western blot; human; 1:1000; loading ...; fig 1a
Novus Biologicals H2AX antibody (Noves, NB100-74435) was used in western blot on human samples at 1:1000 (fig 1a). Front Physiol (2021) ncbi
mouse monoclonal (3F2)
  • immunohistochemistry - frozen section; mouse; loading ...; fig 2b
In order to study the role of cell adhesion molecule BT-IgSF in the blood-testis barrier and male fertility, Novus Biologicals H2AX antibody (Novus Biologicals, NB100-74435) was used in immunohistochemistry - frozen section on mouse samples (fig 2b). J Biol Chem (2017) ncbi
Articles Reviewed
  1. Sahm V, Maurer C, Baumeister T, Anand A, Strangmann J, Schmid R, et al. Telomere shortening accelerates tumor initiation in the L2-IL1B mouse model of Barrett esophagus and emerges as a possible biomarker. Oncotarget. 2022;13:347-359 pubmed publisher
  2. Cao C, Tian B, Geng X, Zhou H, Xu Z, Lai T, et al. IL-17-Mediated Inflammation Promotes Cigarette Smoke-Induced Genomic Instability. Cells. 2021;10: pubmed publisher
  3. Kildey K, Gandhi N, Sahin K, Shah E, Boittier E, Duijf P, et al. Elevating CDCA3 levels in non-small cell lung cancer enhances sensitivity to platinum-based chemotherapy. Commun Biol. 2021;4:638 pubmed publisher
  4. Pramanick A, Chakraborti S, Mahata T, Basak M, Das K, Verma S, et al. G protein β5-ATM complexes drive acetaminophen-induced hepatotoxicity. Redox Biol. 2021;43:101965 pubmed publisher
  5. Jia Y, Kang X, Tan L, Ren Y, Qu L, Tang J, et al. Nicotinamide Mononucleotide Attenuates Renal Interstitial Fibrosis After AKI by Suppressing Tubular DNA Damage and Senescence. Front Physiol. 2021;12:649547 pubmed publisher
  6. Zhu X, Chen L, Huang B, Li X, Yang L, Hu X, et al. Efficacy and mechanism of the combination of PARP and CDK4/6 inhibitors in the treatment of triple-negative breast cancer. J Exp Clin Cancer Res. 2021;40:122 pubmed publisher
  7. Wu Y, Dong J, Feng S, Zhao Q, Duan P, Xiong M, et al. Maternal UHRF1 Is Essential for Transcription Landscapes and Repression of Repetitive Elements During the Maternal-to-Zygotic Transition. Front Cell Dev Biol. 2020;8:610773 pubmed publisher
  8. He S, Ma X, Zheng N, Wang G, Wang M, Xia W, et al. PRDM14 mediates chemosensitivity and glycolysis in drug‑resistant A549/cisplatin cells and their progenitor A549 human lung adenocarcinoma cells. Mol Med Rep. 2021;23: pubmed publisher
  9. Fu E, Liu T, Yu S, Chen X, Song L, Lou H, et al. M2 macrophages reduce the radiosensitivity of head and neck cancer by releasing HB‑EGF. Oncol Rep. 2020;44:698-710 pubmed publisher
  10. Jansons J, Bayurova E, Skrastina D, Kurlanda A, Fridrihsone I, Kostyushev D, et al. Expression of the Reverse Transcriptase Domain of Telomerase Reverse Transcriptase Induces Lytic Cellular Response in DNA-Immunized Mice and Limits Tumorigenic and Metastatic Potential of Murine Adenocarcinoma 4T1 Cells. Vaccines (Basel). 2020;8: pubmed publisher
  11. Hastings J, González Rajal A, Latham S, Han J, McCloy R, O Donnell Y, et al. Analysis of pulsed cisplatin signalling dynamics identifies effectors of resistance in lung adenocarcinoma. elife. 2020;9: pubmed publisher
  12. Ma H, Qian W, Bambousková M, Collins P, Porter S, Byrum A, et al. Barrier-to-Autointegration Factor 1 Protects against a Basal cGAS-STING Response. MBio. 2020;11: pubmed publisher
  13. Liu H, Liu Y, Yang F, Zhang L, Zhang F, Hu X, et al. Acetylation of MORC2 by NAT10 regulates cell-cycle checkpoint control and resistance to DNA-damaging chemotherapy and radiotherapy in breast cancer. Nucleic Acids Res. 2020;48:3638-3656 pubmed publisher
  14. Wall C, Rose C, Adrian M, Zeng Y, Kirkpatrick D, Bingol B. PPEF2 Opposes PINK1-Mediated Mitochondrial Quality Control by Dephosphorylating Ubiquitin. Cell Rep. 2019;29:3280-3292.e7 pubmed publisher
  15. Varuzhanyan G, Rojansky R, Sweredoski M, Graham R, Hess S, Ladinsky M, et al. Mitochondrial fusion is required for spermatogonial differentiation and meiosis. elife. 2019;8: pubmed publisher
  16. Moreno Blas D, Gorostieta Salas E, Pommer Alba A, Muciño Hernández G, Gerónimo Olvera C, Maciel Barón L, et al. Cortical neurons develop a senescence-like phenotype promoted by dysfunctional autophagy. Aging (Albany NY). 2019;11:6175-6198 pubmed publisher
  17. Jia Z, Nie Y, Yue F, Kong Y, Gu L, Gavin T, et al. A requirement of Polo-like kinase 1 in murine embryonic myogenesis and adult muscle regeneration. elife. 2019;8: pubmed publisher
  18. Walton C, Zhang W, Patiño Parrado I, Barrio Alonso E, Garrido J, Frade J. Primary neurons can enter M-phase. Sci Rep. 2019;9:4594 pubmed publisher
  19. Bartova E, Lochmanová G, Legartova S, Suchankova J, Fedr R, Krejci J, et al. Irradiation by γ-rays reduces the level of H3S10 phosphorylation and weakens the G2 phase-dependent interaction between H3S10 phosphorylation and γH2AX. Biochimie. 2018;154:86-98 pubmed publisher
  20. Kannan A, Bhatia K, Branzei D, Gangwani L. Combined deficiency of Senataxin and DNA-PKcs causes DNA damage accumulation and neurodegeneration in spinal muscular atrophy. Nucleic Acids Res. 2018;46:8326-8346 pubmed publisher
  21. Pelz L, Purfürst B, Rathjen F. The cell adhesion molecule BT-IgSF is essential for a functional blood-testis barrier and male fertility in mice. J Biol Chem. 2017;292:21490-21503 pubmed publisher
  22. Xiang Y, Laurent B, Hsu C, Nachtergaele S, Lu Z, Sheng W, et al. RNA m6A methylation regulates the ultraviolet-induced DNA damage response. Nature. 2017;543:573-576 pubmed publisher
  23. Song X, Narzt M, Nagelreiter I, Hohensinner P, Terlecki Zaniewicz L, Tschachler E, et al. Autophagy deficient keratinocytes display increased DNA damage, senescence and aberrant lipid composition after oxidative stress in vitro and in vivo. Redox Biol. 2017;11:219-230 pubmed publisher
  24. Hu M, Chen S, Wang B, Ou T, Gu L, Tan J, et al. Specific targeting of telomeric multimeric G-quadruplexes by a new triaryl-substituted imidazole. Nucleic Acids Res. 2017;45:1606-1618 pubmed publisher
  25. York D, Withers S, Watson K, Seo K, Rebhun R. Enrofloxacin enhances the effects of chemotherapy in canine osteosarcoma cells with mutant and wild-type p53. Vet Comp Oncol. 2017;15:1087-1100 pubmed publisher
  26. Yang Q, Pan Q, Li C, Xu Y, Wen C, Sun F. NRAGE is involved in homologous recombination repair to resist the DNA-damaging chemotherapy and composes a ternary complex with RNF8-BARD1 to promote cell survival in squamous esophageal tumorigenesis. Cell Death Differ. 2016;23:1406-16 pubmed publisher
  27. Bunch H, Lawney B, Lin Y, Asaithamby A, Murshid A, Wang Y, et al. Transcriptional elongation requires DNA break-induced signalling. Nat Commun. 2015;6:10191 pubmed publisher
  28. Ouyang S, Song Y, Tian Y, Chen Y, Yu X, Wang D. RNF8 deficiency results in neurodegeneration in mice. Neurobiol Aging. 2015;36:2850-2860 pubmed publisher
  29. Nadeau M, Rico C, Tsoi M, Vivancos M, Filimon S, Paquet M, et al. Pharmacological targeting of valosin containing protein (VCP) induces DNA damage and selectively kills canine lymphoma cells. BMC Cancer. 2015;15:479 pubmed publisher
  30. Yang K, Kohler R, Landon M, Giedt R, Weissleder R. Single cell resolution in vivo imaging of DNA damage following PARP inhibition. Sci Rep. 2015;5:10129 pubmed publisher
  31. Huang S, Cui Y, Guo X, Wang L, Li S, Lu Y, et al. 2,2',4,4'-Tetrabromodiphenyl ether disrupts spermatogenesis, impairs mitochondrial function and induces apoptosis of early leptotene spermatocytes in rats. Reprod Toxicol. 2015;51:114-24 pubmed publisher
  32. Mohan M, Kumar V, Lackner A, Alvarez X. Dysregulated miR-34a-SIRT1-acetyl p65 axis is a potential mediator of immune activation in the colon during chronic simian immunodeficiency virus infection of rhesus macaques. J Immunol. 2015;194:291-306 pubmed publisher
  33. Flassig R, Maubach G, Täger C, Sundmacher K, Naumann M. Experimental design, validation and computational modeling uncover DNA damage sensing by DNA-PK and ATM. Mol Biosyst. 2014;10:1978-86 pubmed publisher
  34. Sutherland J, Fraser B, Sobinoff A, Pye V, Davidson T, Siddall N, et al. Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression. Biol Reprod. 2014;90:92 pubmed publisher
  35. Di Giacomo M, Comazzetto S, Saini H, De Fazio S, Carrieri C, Morgan M, et al. Multiple epigenetic mechanisms and the piRNA pathway enforce LINE1 silencing during adult spermatogenesis. Mol Cell. 2013;50:601-8 pubmed publisher
  36. He B, Mi Y, Zhang C. Gonadotropins regulate ovarian germ cell mitosis/meiosis decision in the embryonic chicken. Mol Cell Endocrinol. 2013;370:32-41 pubmed publisher