This is a Validated Antibody Database (VAD) review about bovine ACAN, based on 22 published articles (read how Labome selects the articles), using ACAN antibody in all methods. It is aimed to help Labome visitors find the most suited ACAN antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
ACAN synonym: AGC1

Abcam
mouse monoclonal (6-B-4)
  • western blot; human; 1:1000; loading ...; fig 2c
Abcam ACAN antibody (Abcam, ab3778) was used in western blot on human samples at 1:1000 (fig 2c). Aging (Albany NY) (2021) ncbi
mouse monoclonal (6-B-4)
  • western blot; human; loading ...; fig 1e
Abcam ACAN antibody (Abcam, ab3778) was used in western blot on human samples (fig 1e). Cell Death Dis (2021) ncbi
mouse monoclonal (6-B-4)
  • immunohistochemistry; rat; 1:400; loading ...; fig 2f
  • immunocytochemistry; mouse; loading ...; fig 3b
Abcam ACAN antibody (Abcam, ab3778) was used in immunohistochemistry on rat samples at 1:400 (fig 2f) and in immunocytochemistry on mouse samples (fig 3b). Arthritis Res Ther (2021) ncbi
mouse monoclonal (6-B-4)
  • western blot; mouse; loading ...; fig 3a
Abcam ACAN antibody (Abcam, ab3778) was used in western blot on mouse samples (fig 3a). Cell Death Dis (2021) ncbi
mouse monoclonal (6-B-4)
  • western blot; human; 1:1000; fig 7
Abcam ACAN antibody (Abcam, ab3778) was used in western blot on human samples at 1:1000 (fig 7). Aging (Albany NY) (2020) ncbi
mouse monoclonal (6-B-4)
  • immunohistochemistry; human; 1:300; fig 6
Abcam ACAN antibody (Abcam, ab3778) was used in immunohistochemistry on human samples at 1:300 (fig 6). BMC Biotechnol (2020) ncbi
mouse monoclonal (6-B-4)
  • western blot; mouse; 1:500; loading ...; fig 4b
Abcam ACAN antibody (Abcam, ab3778) was used in western blot on mouse samples at 1:500 (fig 4b). Aging (Albany NY) (2020) ncbi
mouse monoclonal (6-B-4)
  • western blot; human; 1:100; fig 5
Abcam ACAN antibody (Abcam, ab3778) was used in western blot on human samples at 1:100 (fig 5). Sci Rep (2016) ncbi
mouse monoclonal (6-B-4)
  • immunocytochemistry; bovine; 1:50; loading ...; fig 2a
Abcam ACAN antibody (Abcam, ab3778) was used in immunocytochemistry on bovine samples at 1:50 (fig 2a). Nat Commun (2016) ncbi
mouse monoclonal (6-B-4)
  • immunocytochemistry; human; 1:100; loading ...; fig 3c
Abcam ACAN antibody (Abcam, ab3778) was used in immunocytochemistry on human samples at 1:100 (fig 3c). Int J Mol Med (2015) ncbi
mouse monoclonal (6-B-4)
  • immunohistochemistry; mouse
Abcam ACAN antibody (Abcam, ab3778) was used in immunohistochemistry on mouse samples . PLoS ONE (2014) ncbi
Invitrogen
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:50; loading ...; fig 6a
Invitrogen ACAN antibody (Thermo Scientific, PA1-1746) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 6a). Arthritis Rheumatol (2022) ncbi
mouse monoclonal (BC-3)
  • immunocytochemistry; human; 1:200; loading ...; fig 1g
Invitrogen ACAN antibody (Thermo Fisher Scientific, MA3-16888) was used in immunocytochemistry on human samples at 1:200 (fig 1g). Cell Res (2020) ncbi
domestic rabbit polyclonal
  • western blot; human; 1 ug/ml; loading ...; fig s3b
Invitrogen ACAN antibody (Thermo Fisher, PA1-1746) was used in western blot on human samples at 1 ug/ml (fig s3b). Arterioscler Thromb Vasc Biol (2019) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry; horses; 1:30; loading ...; tbl 1
In order to compare 3D co-culture constructs of primary equine mesenchymal stem cells and meniscus cells for their qualification to be applied for meniscus tissue engineering, Invitrogen ACAN antibody (Affinity Bioreagents, MA3- 16888) was used in immunohistochemistry on horses samples at 1:30 (tbl 1). Tissue Eng Part A (2017) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:500; fig 3
In order to determine the effects of hydroxytyrosol and procyanidin treatment on osteoarthritis using rabbits, Invitrogen ACAN antibody (Thermo Fisher Scientific, PA1-1746) was used in immunohistochemistry - paraffin section on mouse samples at 1:500 (fig 3). Sci Rep (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - paraffin section; rat; 1:100; fig 7
  • immunohistochemistry - paraffin section; pigs ; 1:100; fig 7
In order to utilize a decellularized cartilage-based hydrogel to help with the compressive modulus of articular cartilage, Invitrogen ACAN antibody (ThermoFisher Scientific, MA3-16888) was used in immunohistochemistry - paraffin section on rat samples at 1:100 (fig 7) and in immunohistochemistry - paraffin section on pigs samples at 1:100 (fig 7). Acta Biomater (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - frozen section; pigs ; 1:100; fig 8
In order to compare devitalized and decellularized cartilage encapsulated in hydrogel pastes as chondroinduction from naturally derived cartilage matrix, Invitrogen ACAN antibody (ThermoFisher, MA3-16888) was used in immunohistochemistry - frozen section on pigs samples at 1:100 (fig 8). Tissue Eng Part A (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - paraffin section; zebrafish ; fig 5
In order to analyze how Cx43-dependent skeletal phenotypes are mediated by interactions between Sema3d and HapIn1a-ECM during regeneration of fins, Invitrogen ACAN antibody (Thermo Scientific, MA3-16888) was used in immunohistochemistry - paraffin section on zebrafish samples (fig 5). PLoS ONE (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - frozen section; pigs ; 1:100; fig 9
  • immunohistochemistry - frozen section; rat; 1:100; fig 9
In order to research naturally derived devitalized cartilage used as chondroinductive hydrogel pastes, Invitrogen ACAN antibody (ThermoFisher, MA3-16888) was used in immunohistochemistry - frozen section on pigs samples at 1:100 (fig 9) and in immunohistochemistry - frozen section on rat samples at 1:100 (fig 9). Ann Biomed Eng (2016) ncbi
domestic rabbit polyclonal
  • immunohistochemistry - paraffin section; mouse; 1:50; fig 2
In order to analyze development of experimental osteoarthritis and aggrecan degradation in murine articular cartilage by JNK-2, Invitrogen ACAN antibody (Thermo Scientific, PA1-1746) was used in immunohistochemistry - paraffin section on mouse samples at 1:50 (fig 2). Arthritis Rheumatol (2016) ncbi
mouse monoclonal (BC-3)
  • immunohistochemistry - frozen section; zebrafish ; fig 1c
In order to analyze extracellular matrix protein expression during caudal fin regeneration in zebrafish, Invitrogen ACAN antibody (Thermo Scientific, MA3-16888) was used in immunohistochemistry - frozen section on zebrafish samples (fig 1c). Gene Expr Patterns (2015) ncbi
Articles Reviewed
  1. Liu Y, Zhang Y. Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction. Aging (Albany NY). 2021;13:20383-20394 pubmed publisher
  2. Coveney C, Zhu L, Miotla Zarebska J, Stott B, Parisi I, Batchelor V, et al. Role of Ciliary Protein Intraflagellar Transport Protein 88 in the Regulation of Cartilage Thickness and Osteoarthritis Development in Mice. Arthritis Rheumatol. 2022;74:49-59 pubmed publisher
  3. Hou M, Zhang Y, Zhou X, Liu T, Yang H, Chen X, et al. Kartogenin prevents cartilage degradation and alleviates osteoarthritis progression in mice via the miR-146a/NRF2 axis. Cell Death Dis. 2021;12:483 pubmed publisher
  4. Wang W, Zhu Y, Sun Z, Jin C, Wang X. Positive feedback regulation between USP15 and ERK2 inhibits osteoarthritis progression through TGF-β/SMAD2 signaling. Arthritis Res Ther. 2021;23:84 pubmed publisher
  5. Guo Q, Chen X, Chen J, Zheng G, Xie C, Wu H, et al. STING promotes senescence, apoptosis, and extracellular matrix degradation in osteoarthritis via the NF-κB signaling pathway. Cell Death Dis. 2021;12:13 pubmed publisher
  6. Wang L, Yang M, Zhang C, Huang F. The protective effects of dehydrocostus lactone against TNF-α-induced degeneration of extracellular matrix (ECM) in SW1353 cells. Aging (Albany NY). 2020;12:17137-17149 pubmed publisher
  7. Huang Y, Seitz D, Chevalier Y, Müller P, Jansson V, Klar R. Synergistic interaction of hTGF-β3 with hBMP-6 promotes articular cartilage formation in chitosan scaffolds with hADSCs: implications for regenerative medicine. BMC Biotechnol. 2020;20:48 pubmed publisher
  8. Xu Z, Ke T, Zhang Y, Fu C, He W. Agonism of GPR120 prevented IL-1β-induced reduction of extracellular matrix through SOX-9. Aging (Albany NY). 2020;12:12074-12085 pubmed publisher
  9. Wu J, Song D, Li Z, Guo B, Xiao Y, Liu W, et al. Immunity-and-matrix-regulatory cells derived from human embryonic stem cells safely and effectively treat mouse lung injury and fibrosis. Cell Res. 2020;30:794-809 pubmed publisher
  10. Yin 殷晓科 X, Wanga S, Fellows A, Barallobre Barreiro J, Lu R, Davaapil H, et al. Glycoproteomic Analysis of the Aortic Extracellular Matrix in Marfan Patients. Arterioscler Thromb Vasc Biol. 2019;39:1859-1873 pubmed publisher
  11. Kremer A, Ribitsch I, Reboredo J, Dürr J, Egerbacher M, Jenner F, et al. Three-Dimensional Coculture of Meniscal Cells and Mesenchymal Stem Cells in Collagen Type I Hydrogel on a Small Intestinal Matrix-A Pilot Study Toward Equine Meniscus Tissue Engineering. Tissue Eng Part A. 2017;23:390-402 pubmed publisher
  12. Mével E, Merceron C, Vinatier C, Krisa S, Richard T, Masson M, et al. Olive and grape seed extract prevents post-traumatic osteoarthritis damages and exhibits in vitro anti IL-1? activities before and after oral consumption. Sci Rep. 2016;6:33527 pubmed publisher
  13. Wu X, Liu W, Duan Z, Gao Y, Li S, Wang K, et al. The Involvement of Protease Nexin-1 (PN1) in the Pathogenesis of Intervertebral Disc (IVD) Degeneration. Sci Rep. 2016;6:30563 pubmed publisher
  14. Beck E, Barragan M, Tadros M, Gehrke S, Detamore M. Approaching the compressive modulus of articular cartilage with a decellularized cartilage-based hydrogel. Acta Biomater. 2016;38:94-105 pubmed publisher
  15. Beck E, Barragan M, Libeer T, Kieweg S, Converse G, Hopkins R, et al. Chondroinduction from Naturally Derived Cartilage Matrix: A Comparison Between Devitalized and Decellularized Cartilage Encapsulated in Hydrogel Pastes. Tissue Eng Part A. 2016;22:665-79 pubmed publisher
  16. Cote A, McLeod C, Farrell M, McClanahan P, Dunagin M, Raj A, et al. Single-cell differences in matrix gene expression do not predict matrix deposition. Nat Commun. 2016;7:10865 pubmed publisher
  17. Govindan J, Tun K, Iovine M. Cx43-Dependent Skeletal Phenotypes Are Mediated by Interactions between the Hapln1a-ECM and Sema3d during Fin Regeneration. PLoS ONE. 2016;11:e0148202 pubmed publisher
  18. Beck E, Barragan M, Tadros M, Kiyotake E, Acosta F, Kieweg S, et al. Chondroinductive Hydrogel Pastes Composed of Naturally Derived Devitalized Cartilage. Ann Biomed Eng. 2016;44:1863-80 pubmed publisher
  19. Ismail H, Miotla Zarebska J, Troeberg L, Tang X, Stott B, Yamamoto K, et al. Brief Report: JNK-2 Controls Aggrecan Degradation in Murine Articular Cartilage and the Development of Experimental Osteoarthritis. Arthritis Rheumatol. 2016;68:1165-71 pubmed publisher
  20. Govindan J, Iovine M. Dynamic remodeling of the extra cellular matrix during zebrafish fin regeneration. Gene Expr Patterns. 2015;19:21-9 pubmed publisher
  21. Ma X, Lin Y, Yang K, Yue B, Xiang H, Chen B. Effect of lentivirus-mediated survivin transfection on the morphology and apoptosis of nucleus pulposus cells derived from degenerative human disc in vitro. Int J Mol Med. 2015;36:186-94 pubmed publisher
  22. Winkler T, Mahoney E, Sinner D, Wylie C, Dahia C. Wnt signaling activates Shh signaling in early postnatal intervertebral discs, and re-activates Shh signaling in old discs in the mouse. PLoS ONE. 2014;9:e98444 pubmed publisher