This is a Validated Antibody Database (VAD) review about Xenopus la.. plk1.S, based on 22 published articles (read how Labome selects the articles), using plk1.S antibody in all methods. It is aimed to help Labome visitors find the most suited plk1.S antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
plk1.S synonym: plk; plk1

Invitrogen
mouse monoclonal (PL6/PL2)
  • western blot; human; 1:500; loading ...; fig 3d
Invitrogen plk1.S antibody (Thermo Fisher, 33-1700) was used in western blot on human samples at 1:500 (fig 3d). EMBO J (2022) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; 1:1000; loading ...; fig 4d
In order to study the effect of environmental and endogenous Toxins on BRCA2 haploinsufficiency and genome instability, Invitrogen plk1.S antibody (ThermoFisher, 33-1700) was used in western blot on human samples at 1:1000 (fig 4d). Cell (2017) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; 1:500; fig s1
In order to investigate stabilization of XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma via USP9X, Invitrogen plk1.S antibody (Invitrogen, 33-1700) was used in western blot on human samples at 1:500 (fig s1). EMBO Mol Med (2016) ncbi
mouse monoclonal (PL6/PL2)
  • immunoprecipitation; human; fig 4
  • western blot; human; fig 4
In order to investigate the role of topoisomerase IIbeta-binding protein 1 in DNA repair and its contribution to cancer, Invitrogen plk1.S antibody (Zymed, 331700) was used in immunoprecipitation on human samples (fig 4) and in western blot on human samples (fig 4). J Cell Biol (2016) ncbi
mouse monoclonal (PL6/PL2)
In order to analyze G2 checkpoint regulators for hypoxia-induced alterations, Invitrogen plk1.S antibody (Zymed, Zy-1700) was used . Mol Oncol (2016) ncbi
mouse monoclonal (PL6/PL2)
  • other; human; loading ...; fig st1
In order to use size exclusion chromatography-microsphere-based affinity proteomics to study clinical samples obtained from pediatric acute leukemia patients, Invitrogen plk1.S antibody (INVITROGEN, PL6 and PL2) was used in other on human samples (fig st1). Mol Cell Proteomics (2016) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; 1:500; fig 2
In order to assess the regulation of PLK1 and PCNT cleavage and mitotic exit via centriole separation, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in western blot on human samples at 1:500 (fig 2). Nat Commun (2015) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; mouse; fig 1
In order to elucidate how PLK1 regulates cell survival during mTORC1 hyperactivation, Invitrogen plk1.S antibody (Invitrogen, 33-1700) was used in western blot on mouse samples (fig 1). Cell Cycle (2015) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human
In order to demonstrate that SNW1 and PRPF8 are essential for sister chromatid cohesion in human cells, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in western blot on human samples . EMBO J (2014) ncbi
mouse monoclonal (PL6/PL2)
  • immunoprecipitation; human; fig 2
  • western blot; human; fig 2
In order to report how NFBD1 and PLK1 regulate the G2/M transition, Invitrogen plk1.S antibody (Zymed, PL2) was used in immunoprecipitation on human samples (fig 2) and in western blot on human samples (fig 2). PLoS ONE (2013) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; 1:500; fig 5
In order to study the effect of human papillomavirus type 16 E7 oncoprotein on the anaphase promoting complex/cyclosome and its mechanism, Invitrogen plk1.S antibody (Invitrogen, 33-1700) was used in western blot on human samples at 1:500 (fig 5). Virology (2013) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; fig 7
In order to study the mechanism by which human Cdc14A regulates the stability of Wee1, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in western blot on human samples (fig 7). Mol Biol Cell (2012) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; 1:300
In order to show that mitosis is a critical step in the cellular response to genotoxic agents in human cells, Invitrogen plk1.S antibody (Zymed Laboratories, 33-1700) was used in western blot on human samples at 1:300. Biochem J (2012) ncbi
mouse monoclonal (PL6/PL2)
  • immunocytochemistry; human; fig 1
  • western blot; human; fig 2
In order to study phosphorylation of RanBP1 by Plk1 during mitosis, Invitrogen plk1.S antibody (Zymed Laboratories, 33-1700) was used in immunocytochemistry on human samples (fig 1) and in western blot on human samples (fig 2). J Biol Chem (2011) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; fig 1
In order to investigate anaphase promoting complex during normal and abnormal mitotic processes, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in western blot on human samples (fig 1). Cell Cycle (2010) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; fig 1
In order to assess the role of p21(WAF1) in G2-M phase checkpoint control and in prevention of polyploidy after DNA damage, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in western blot on human samples (fig 1). Mol Biol Cell (2009) ncbi
mouse monoclonal (PL6/PL2)
  • immunoprecipitation; human; fig 2b
In order to characterize the interaction between TRF1 and Plk1, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in immunoprecipitation on human samples (fig 2b). J Biol Chem (2008) ncbi
mouse monoclonal (PL6/PL2)
  • immunoprecipitation; human
  • western blot; human
In order to report PLK1-mediated and phosphor-dependent transcriptional deactivation of TAp73, Invitrogen plk1.S antibody (Zymed Laboratories, 33-1700) was used in immunoprecipitation on human samples and in western blot on human samples . Cell Cycle (2008) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; fig 2
In order to assess if histone deacetylase inhibitors affect radiation-induced tumor cell death, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in western blot on human samples (fig 2). Radiat Oncol (2006) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; fig 1B
In order to determine the gene expression profiles of irradiated Reh and U698 cells, Invitrogen plk1.S antibody (Zymed, 33-170) was used in western blot on human samples (fig 1B). Int J Cancer (2005) ncbi
mouse monoclonal (PL6/PL2)
  • immunocytochemistry; human; 5 ug/ml; fig 6
  • western blot; human; fig 2B
In order to report that Plk1 is required for Emi1 destruction during mitosis, Invitrogen plk1.S antibody (Zymed, 33-1700) was used in immunocytochemistry on human samples at 5 ug/ml (fig 6) and in western blot on human samples (fig 2B). Mol Biol Cell (2004) ncbi
mouse monoclonal (PL6/PL2)
  • western blot; human; fig 6
In order to assess global gene expression using p53-deficient human cells to identify genes with G(2)/M-specific and DNA damage-responsive expression, Invitrogen plk1.S antibody (Zymed Laboratories, 33-1700) was used in western blot on human samples (fig 6). J Biol Chem (2001) ncbi
Articles Reviewed
  1. Paulmann C, Spallek R, Karpiuk O, Heider M, Sch xe4 ffer I, Zecha J, et al. The OTUD6B-LIN28B-MYC axis determines the proliferative state in multiple myeloma. EMBO J. 2022;41:e110871 pubmed publisher
  2. Tan S, Chadha S, Liu Y, Gabasova E, Perera D, Ahmed K, et al. A Class of Environmental and Endogenous Toxins Induces BRCA2 Haploinsufficiency and Genome Instability. Cell. 2017;169:1105-1118.e15 pubmed publisher
  3. Engel K, Rudelius M, Slawska J, Jacobs L, Ahangarian Abhari B, Altmann B, et al. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. EMBO Mol Med. 2016;8:851-62 pubmed publisher
  4. Moudry P, Watanabe K, Wolanin K, Bartkova J, Wassing I, Watanabe S, et al. TOPBP1 regulates RAD51 phosphorylation and chromatin loading and determines PARP inhibitor sensitivity. J Cell Biol. 2016;212:281-8 pubmed publisher
  5. Hasvold G, Lund Andersen C, Lando M, Patzke S, Hauge S, Suo Z, et al. Hypoxia-induced alterations of G2 checkpoint regulators. Mol Oncol. 2016;10:764-73 pubmed publisher
  6. Kanderová V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, et al. High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016;15:1246-61 pubmed publisher
  7. Kim J, Lee K, Rhee K. PLK1 regulation of PCNT cleavage ensures fidelity of centriole separation during mitotic exit. Nat Commun. 2015;6:10076 pubmed publisher
  8. Valianou M, Cox A, Pichette B, Hartley S, Paladhi U, Astrinidis A. Pharmacological inhibition of Polo-like kinase 1 (PLK1) by BI-2536 decreases the viability and survival of hamartin and tuberin deficient cells via induction of apoptosis and attenuation of autophagy. Cell Cycle. 2015;14:399-407 pubmed publisher
  9. van der Lelij P, Stocsits R, Ladurner R, Petzold G, Kreidl E, Koch B, et al. SNW1 enables sister chromatid cohesion by mediating the splicing of sororin and APC2 pre-mRNAs. EMBO J. 2014;33:2643-58 pubmed publisher
  10. Ando K, Ozaki T, Hirota T, Nakagawara A. NFBD1/MDC1 is phosphorylated by PLK1 and controls G2/M transition through the regulation of a TOPOII?-mediated decatenation checkpoint. PLoS ONE. 2013;8:e82744 pubmed publisher
  11. Yu Y, Munger K. Human papillomavirus type 16 E7 oncoprotein inhibits the anaphase promoting complex/cyclosome activity by dysregulating EMI1 expression in mitosis. Virology. 2013;446:251-9 pubmed publisher
  12. Ovejero S, Ayala P, Bueno A, Sacristán M. Human Cdc14A regulates Wee1 stability by counteracting CDK-mediated phosphorylation. Mol Biol Cell. 2012;23:4515-25 pubmed publisher
  13. Kubara P, Kernéis Golsteyn S, Studény A, Lanser B, Meijer L, Golsteyn R. Human cells enter mitosis with damaged DNA after treatment with pharmacological concentrations of genotoxic agents. Biochem J. 2012;446:373-81 pubmed publisher
  14. Hwang H, Ji J, Jang Y. Phosphorylation of Ran-binding protein-1 by Polo-like kinase-1 is required for interaction with Ran and early mitotic progression. J Biol Chem. 2011;286:33012-20 pubmed publisher
  15. Lee J, Kim J, Margolis R, Fotedar R. Substrate degradation by the anaphase promoting complex occurs during mitotic slippage. Cell Cycle. 2010;9:1792-801 pubmed
  16. Lee J, Kim J, Barbier V, Fotedar A, Fotedar R. DNA damage triggers p21WAF1-dependent Emi1 down-regulation that maintains G2 arrest. Mol Biol Cell. 2009;20:1891-902 pubmed publisher
  17. Wu Z, Yang X, Weber G, Liu X. Plk1 phosphorylation of TRF1 is essential for its binding to telomeres. J Biol Chem. 2008;283:25503-13 pubmed publisher
  18. Soond S, Barry S, Melino G, Knight R, Latchman D, Stephanou A. p73-mediated transcriptional activity is negatively regulated by polo-like kinase 1. Cell Cycle. 2008;7:1214-23 pubmed
  19. Flatmark K, Nome R, Folkvord S, Bratland A, Rasmussen H, Ellefsen M, et al. Radiosensitization of colorectal carcinoma cell lines by histone deacetylase inhibition. Radiat Oncol. 2006;1:25 pubmed
  20. Lyng H, Landsverk K, Kristiansen E, DeAngelis P, Ree A, Myklebost O, et al. Response of malignant B lymphocytes to ionizing radiation: gene expression and genotype. Int J Cancer. 2005;115:935-42 pubmed
  21. Hansen D, Loktev A, Ban K, Jackson P. Plk1 regulates activation of the anaphase promoting complex by phosphorylating and triggering SCFbetaTrCP-dependent destruction of the APC Inhibitor Emi1. Mol Biol Cell. 2004;15:5623-34 pubmed
  22. Crawford D, Piwnica Worms H. The G(2) DNA damage checkpoint delays expression of genes encoding mitotic regulators. J Biol Chem. 2001;276:37166-77 pubmed